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P14142 (GTR4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 2, facilitated glucose transporter member 4
Alternative name(s):
GT2
Glucose transporter type 4, insulin-responsive
Short name=GLUT-4
Gene names
Name:Slc2a4
Synonyms:Glut-4, Glut4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Insulin-regulated facilitative glucose transporter.

Subunit structure

Binds to DAXX. Interacts via its N-terminus with SRFBP1. Interacts with NDUFA9 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein By similarity. Cytoplasmperinuclear region By similarity. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration By similarity. Ref.10

Tissue specificity

Skeletal and cardiac muscles; brown and white fat.

Involvement in disease

Defects in Slc2a4 may be the cause of certain post-receptor defects in non-insulin-dependent diabetes mellitus (NIDDM).

Miscellaneous

Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation.

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]

Ontologies

Keywords
   Biological processSugar transport
Transport
   Cellular componentCell membrane
Cytoplasm
Membrane
   DiseaseDiabetes mellitus
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processamylopectin biosynthetic process

Inferred from direct assay PubMed 14562105. Source: MGI

brown fat cell differentiation

Inferred from direct assay PubMed 18492766. Source: MGI

cellular response to insulin stimulus

Inferred from direct assay Ref.10. Source: UniProtKB

cellular response to osmotic stress

Inferred from direct assay PubMed 18227281. Source: MGI

glucose homeostasis

Inferred from electronic annotation. Source: Ensembl

glucose transport

Inferred from direct assay PubMed 12097321PubMed 15857891. Source: MGI

response to ethanol

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcoated pit

Inferred from electronic annotation. Source: Ensembl

cytoplasmic vesicle membrane

Inferred from direct assay PubMed 12490950. Source: MGI

endomembrane system

Inferred from direct assay Ref.10. Source: UniProtKB

endosome

Inferred from direct assay PubMed 15247266. Source: MGI

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

extracellular vesicular exosome

Inferred from direct assay PubMed 19724054. Source: MGI

insulin-responsive compartment

Inferred from direct assay Ref.10. Source: UniProtKB

integral component of membrane

Inferred from direct assay PubMed 14562105. Source: MGI

integral component of plasma membrane

Inferred from direct assay PubMed 14562105. Source: MGI

membrane

Inferred from direct assay PubMed 16455755PubMed 23041416. Source: MGI

multivesicular body

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from direct assay PubMed 10444069PubMed 15800058. Source: MGI

plasma membrane

Inferred from direct assay Ref.10. Source: UniProtKB

sarcolemma

Inferred from direct assay PubMed 16455755. Source: MGI

trans-Golgi network transport vesicle

Inferred from direct assay PubMed 17003038. Source: MGI

   Molecular_functionD-glucose transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

glucose transmembrane transporter activity

Inferred from direct assay PubMed 12097321. Source: MGI

insulin-responsive hydrogen:glucose symporter activity

Traceable author statement PubMed 7675081. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Solute carrier family 2, facilitated glucose transporter member 4
PRO_0000050364

Regions

Topological domain1 – 2323Cytoplasmic Potential
Transmembrane24 – 4421Helical; Name=1; Potential
Topological domain45 – 8036Extracellular Potential
Transmembrane81 – 10121Helical; Name=2; Potential
Topological domain102 – 1109Cytoplasmic Potential
Transmembrane111 – 13121Helical; Name=3; Potential
Topological domain132 – 14110Extracellular Potential
Transmembrane142 – 16221Helical; Name=4; Potential
Topological domain163 – 1708Cytoplasmic Potential
Transmembrane171 – 19121Helical; Name=5; Potential
Topological domain192 – 2009Extracellular Potential
Transmembrane201 – 22121Helical; Name=6; Potential
Topological domain222 – 28665Cytoplasmic Potential
Transmembrane287 – 30721Helical; Name=7; Potential
Topological domain308 – 32215Extracellular Potential
Transmembrane323 – 34321Helical; Name=8; Potential
Topological domain344 – 3529Cytoplasmic Potential
Transmembrane353 – 37321Helical; Name=9; Potential
Topological domain374 – 38411Extracellular Potential
Transmembrane385 – 40521Helical; Name=10; Potential
Topological domain406 – 41611Cytoplasmic Potential
Transmembrane417 – 43721Helical; Name=11; Potential
Topological domain438 – 4447Extracellular Potential
Transmembrane445 – 46521Helical; Name=12; Potential
Topological domain466 – 50843Cytoplasmic Potential
Region7 – 137SRFBP1-binding By similarity
Region294 – 2963Defines substrate specificity By similarity
Motif489 – 4902Dileucine internalization motif Potential

Amino acid modifications

Modified residue2741Phosphoserine; by SGK1 By similarity
Glycosylation571N-linked (GlcNAc...) Ref.9

Experimental info

Sequence conflict111D → DVK in AAA37753. Ref.1
Sequence conflict1321A → V in AAA37753. Ref.1
Sequence conflict1771Q → R in AAA37753. Ref.1
Sequence conflict2461R → P in AAA37753. Ref.1
Sequence conflict4061Missing in AAA37753. Ref.1
Sequence conflict4441A → R in AAA37753. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14142 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: A174212455EE0390

FASTA50954,755
        10         20         30         40         50         60 
MPSGFQQIGS DDGEPPRQRV TGTLVLAVFS AVLGSLQFGY NIGVINAPQK VIEQSYNATW 

        70         80         90        100        110        120 
LGRQGPGGPD SIPQGTLTTL WALSVAIFSV GGMISSFLIG IISQWLGRKR AMLANNVLAV 

       130        140        150        160        170        180 
LGGALMGLAN AAASYEILIL GRFLIGAYSG LTSGLVPMYV GEIAPTHLRG ALGTLNQLAI 

       190        200        210        220        230        240 
VIGILVAQVL GLESMLGTAT LWPLLLALTV LPALLQLILL PFCPESPRYL YIIRNLEGPA 

       250        260        270        280        290        300 
RKSLKRLTGW ADVSDALAEL KDEKRKLERE RPMSLLQLLG SRTHRQPLII AVVLQLSQQL 

       310        320        330        340        350        360 
SGINAVFYYS TSIFESAGVG QPAYATIGAG VVNTVFTLVS VLLVERAGRR TLHLLGLAGM 

       370        380        390        400        410        420 
CGCAILMTVA LLLLERVPAM SYVSIVAIFG FVAFFEIGPG PIPWFIVAEL FSQGPRPAAM 

       430        440        450        460        470        480 
AVAGFSNWTC NFIVGMGFQY VADAMGPYVF LLFAVLLLGF FIFTFLKVPE TRGRTFDQIS 

       490        500 
AAFRRTPSLL EQEVKPSTEL EYLGPDEND 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, tissue distribution, and differential expression of mRNA for a putative insulin-responsive glucose transporter in mouse 3T3-L1 adipocytes."
Kaestner K.H., Christy R.J., McLenithan J.C., Braiterman L.T., Cornelius P., Pekala P.H., Lane M.D.
Proc. Natl. Acad. Sci. U.S.A. 86:3150-3154(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Age-dependent changes in phenotypes and candidate gene analysis in a polygenic animal model of type II diabetes; NSY mouse."
Ueda H., Ikegami H., Kawaguchi Y., Fujisawa T., Nojima K., Babaya N., Yamada K., Shibata M., Yamato E., Ogihara T.
Diabetologia 43:932-938(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H and NSY.
Tissue: Muscle.
[3]"Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains."
Farber C.R., Corva P.M., Medrano J.F.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CAST/EiJ.
Tissue: Brain.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone and Spinal ganglion.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[8]"Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation."
Sano H., Kane S., Sano E., Miinea C.P., Asara J.M., Lane W.S., Garner C.W., Lienhard G.E.
J. Biol. Chem. 278:14599-14602(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: EFFECT OF TBC1D4 ON GLUT4 TRANSLOCATION.
[9]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-57.
[10]"C2 domain-containing phosphoprotein CDP138 regulates GLUT4 insertion into the plasma membrane."
Xie X., Gong Z., Mansuy-Aubert V., Zhou Q.L., Tatulian S.A., Sehrt D., Gnad F., Brill L.M., Motamedchaboki K., Chen Y., Czech M.P., Mann M., Kruger M., Jiang Z.Y.
Cell Metab. 14:378-389(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M23383 mRNA. Translation: AAA37753.1.
AB008453 mRNA. Translation: BAB03251.1.
AY902342 Genomic DNA. Translation: AAX90627.1.
AK137607 mRNA. Translation: BAE23429.1.
AK164310 mRNA. Translation: BAE37730.1.
AL596185 Genomic DNA. Translation: CAI35157.1.
CH466596 Genomic DNA. Translation: EDL12494.1.
CH466596 Genomic DNA. Translation: EDL12496.1.
BC014282 mRNA. Translation: AAH14282.1.
PIRB30310.
RefSeqNP_033230.2. NM_009204.2.
UniGeneMm.10661.

3D structure databases

ProteinModelPortalP14142.
SMRP14142. Positions 31-473.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203307. 4 interactions.
DIPDIP-42440N.
IntActP14142. 1 interaction.
MINTMINT-1342290.

Chemistry

ChEMBLCHEMBL1250410.

PTM databases

PhosphoSiteP14142.

Proteomic databases

PaxDbP14142.
PRIDEP14142.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000018710; ENSMUSP00000018710; ENSMUSG00000018566.
GeneID20528.
KEGGmmu:20528.
UCSCuc007jsy.1. mouse.

Organism-specific databases

CTD6517.
MGIMGI:95758. Slc2a4.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00720000108560.
HOGENOMHOG000202871.
HOVERGENHBG014816.
InParanoidQ3TPK6.
KOK07191.
OMAAMLVNNI.
OrthoDBEOG7QVM2R.
TreeFamTF313762.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpressP14142.
BgeeP14142.
GenevestigatorP14142.

Family and domain databases

InterProIPR002441. Glc_transpt_4.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR01193. GLUCTRSPORT4.
PR00171. SUGRTRNSPORT.
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298775.
PROP14142.
SOURCESearch...

Entry information

Entry nameGTR4_MOUSE
AccessionPrimary (citable) accession number: P14142
Secondary accession number(s): Q3TPK6, Q9JJN9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot