Reviewed, UniProtKB/Swiss-Prot P14137 (CP11A_RAT)

Names and origin

Protein names

Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    Cytochrome P450 11A1
    CYPXIA1
    P450(scc)
    Cholesterol desmolase

Gene names

Name:	Cyp11a1
Synonyms:	Cyp11a, Cyp11a-1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Catalytic activity	Cholesterol + reduced adrenal ferredoxin + O2 = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Expressed in ovary, adrenal and testis. Ref.4
Induction	Induced by FSH or pregnant mare's serum gonadotropin in ovaries of estrogen-treated immature rats in vivo.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cerebellum development Inferred from expression pattern. Source: RGD cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW granulosa cell differentiation Ref.2 Inferred from expression pattern. Source: RGD mating behavior Inferred from mutant phenotype. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cAMP Ref.2 Inferred from expression pattern. Source: RGD response to cadmium ion Inferred from expression pattern. Source: RGD response to estrogen stimulus Inferred from expression pattern. Source: RGD response to hydrogen peroxide Inferred from mutant phenotype. Source: RGD response to nutrient Inferred from expression pattern. Source: RGD
Cellular component	mitochondrial crista Inferred from direct assay. Source: RGD
Molecular function	cholesterol binding Inferred from mutant phenotype. Source: RGD cholesterol monooxygenase (side-chain-cleaving) activity Inferred from mutant phenotype. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion
	Chain	37 – 526	490	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000003590
Sites
	Metal binding	459	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14137-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: B2930E40018DC1EE	FASTA	526	60,586
	10 20 30 40 50 60 MLAKGLCLRS VLVKSCQPFL SPVWQGPGLA TGNGAGISST NSPRSFNEIP SPGDNGWINL 70 80 90 100 110 120 YHFLRENGTH RIHYHHMQNF QKYGPIYREK LGNMESVYIL DPKDAATLFS CEGPNPERYL 130 140 150 160 170 180 VPPWVAYHQY YQRPIGVLFK SSDAWRKDRI VLNQEVMAPD SIKNFVPLLE GVAQDFIKVL 190 200 210 220 230 240 HRRIKQQNSG KFSGDISDDL FRFAFESITS VVFGERLGML EEIVDPESQR FIDAVYQMFH 250 260 270 280 290 300 TSVPMLNMPP DLFRLFRTKT WKDHAAAWDV IFSKADEYTQ NFYWDLRQKR DFSKYPGVLY 310 320 330 340 350 360 SLLGGNKLPF KNIQANITEM LAGGVDTTSM TLQWNLYEMA HNLKVQEMLR AEVLAARRQA 370 380 390 400 410 420 QGDMAKMVQL VPLLKASIKE TLRLHPISVT LQRYIVNDLV LRNYKIPAKT LVQVASYAMG 430 440 450 460 470 480 RESSFFPNPN KFDPTRWLEK SQNTTHFRYL GFGWGVRQCL GRRIAELEMT IFLINVLENF 490 500 510 520 RIEVQSIRDV GTKFNLILMP EKPIFFNFQP LKQDLGSTMP RKGDTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. cDNA and deduced amino acid sequence of rat P-450scc." Oonk R.B., Krasnow J.S., Beattie W.G., Richards J.S. J. Biol. Chem. 264:21934-21942(1989) [PubMed: 2480959] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Rat cholesterol side-chain cleavage cytochrome P-450 (P-450scc) gene. Structure and regulation by cAMP in vitro." Oonk R.B., Parker K.L., Gibson J.L., Richards J.S. J. Biol. Chem. 265:22392-22401(1990) [PubMed: 2176216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Rat cholesterol side-chain cleavage enzyme (P-450scc): use of a cDNA probe to study the hormonal regulation of P-450scc mRNA levels in ovarian granulosa cells." McMasters K.M., Dickson L.A., Shamy R.V., Robischon K., Macdonald G.J., Moyle W.R. Gene 57:1-9(1987) [PubMed: 3123325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 344-526, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05156 mRNA. Translation: AAA40989.1. M63133 , M63125, M63126, M63127, M63128, M63129, M63130, M63131, M63132 Genomic DNA. Translation: AAA40958.1. BC089100 mRNA. Translation: AAH89100.1. M22615 mRNA. Translation: AAA62267.1.
IPI	IPI00325713.
PIR	A34164.
RefSeq	NP_058982.1.
UniGene	Rn.1401
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Protein-protein interaction databases
STRING	P14137.
Genome annotation databases
Ensembl	ENSRNOT00000010831; ENSRNOP00000010831; ENSRNOG00000008074; Rattus norvegicus. [Genome view]
GeneID	29680.
KEGG	rno:29680.
UCSC	NM_017286. rat.
Organism-specific databases
CTD	29680.
RGD	69325. Cyp11a1.
Phylogenomic databases
HOVERGEN	P14137.
Enzyme and pathway databases
BRENDA	1.14.15.6. 248.
Gene expression databases
ArrayExpress	P14137.
Genevestigator	P14137.
GermOnline	ENSRNOG00000008074. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	610031.

Entry information

Entry name

CP11A_RAT

Accession

Primary (citable) accession number: P14137
Secondary accession number(s): Q5FWY8, Q6LDR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	October 13, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)