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P14137

- CP11A_RAT

UniProt

P14137 - CP11A_RAT

Protein

Cholesterol side-chain cleavage enzyme, mitochondrial

Gene

Cyp11a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

    Catalytic activityi

    Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi459 – 4591Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. cholesterol binding Source: RGD
    2. cholesterol monooxygenase (side-chain-cleaving) activity Source: RGD
    3. heme binding Source: UniProtKB
    4. iron ion binding Source: InterPro

    GO - Biological processi

    1. biphenyl metabolic process Source: RGD
    2. C21-steroid hormone biosynthetic process Source: RGD
    3. cellular response to antibiotic Source: RGD
    4. cellular response to cadmium ion Source: RGD
    5. cellular response to cAMP Source: RGD
    6. cellular response to fibroblast growth factor stimulus Source: RGD
    7. cellular response to follicle-stimulating hormone stimulus Source: RGD
    8. cellular response to gonadotropin stimulus Source: RGD
    9. cellular response to interleukin-1 Source: RGD
    10. cellular response to lipopolysaccharide Source: RGD
    11. cellular response to peptide hormone stimulus Source: RGD
    12. cellular response to transforming growth factor beta stimulus Source: RGD
    13. cellular response to tumor necrosis factor Source: RGD
    14. cerebellum development Source: RGD
    15. cholesterol metabolic process Source: UniProtKB
    16. dibenzo-p-dioxin metabolic process Source: RGD
    17. estrogen biosynthetic process Source: RGD
    18. fractalkine metabolic process Source: RGD
    19. granulosa cell differentiation Source: RGD
    20. hippocampus development Source: RGD
    21. Leydig cell differentiation Source: RGD
    22. male gonad development Source: RGD
    23. maternal process involved in female pregnancy Source: RGD
    24. mating behavior Source: RGD
    25. organic acid metabolic process Source: RGD
    26. phenol-containing compound metabolic process Source: RGD
    27. phthalate metabolic process Source: RGD
    28. progesterone biosynthetic process Source: RGD
    29. response to acid chemical Source: RGD
    30. response to alkaloid Source: RGD
    31. response to antibiotic Source: RGD
    32. response to cadmium ion Source: RGD
    33. response to cAMP Source: RGD
    34. response to corticosterone Source: RGD
    35. response to drug Source: RGD
    36. response to estrogen Source: RGD
    37. response to fungicide Source: RGD
    38. response to gamma radiation Source: RGD
    39. response to genistein Source: RGD
    40. response to gonadotropin Source: RGD
    41. response to hydrogen peroxide Source: RGD
    42. response to insecticide Source: RGD
    43. response to ionizing radiation Source: RGD
    44. response to L-ascorbic acid Source: RGD
    45. response to nutrient Source: RGD
    46. response to organic cyclic compound Source: RGD
    47. response to organic substance Source: RGD
    48. response to peptide hormone Source: RGD
    49. response to salt stress Source: RGD
    50. response to steroid hormone Source: RGD
    51. response to vitamin E Source: RGD
    52. Schwann cell differentiation Source: RGD
    53. steroid biosynthetic process Source: RGD
    54. testosterone biosynthetic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Steroidogenesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP14137.
    UniPathwayiUPA00229.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial (EC:1.14.15.6)
    Alternative name(s):
    CYPXIA1
    Cholesterol desmolase
    Cytochrome P450 11A1
    Cytochrome P450(scc)
    Gene namesi
    Name:Cyp11a1
    Synonyms:Cyp11a, Cyp11a-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi69325. Cyp11a1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial crista Source: RGD
    2. mitochondrial inner membrane Source: RGD
    3. mitochondrion Source: RGD
    4. perikaryon Source: RGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636MitochondrionAdd
    BLAST
    Chaini37 – 526490Cholesterol side-chain cleavage enzyme, mitochondrialPRO_0000003590Add
    BLAST

    Proteomic databases

    PRIDEiP14137.

    Expressioni

    Tissue specificityi

    Expressed in ovary, adrenal and testis.1 Publication

    Inductioni

    Induced by FSH or pregnant mare's serum gonadotropin in ovaries of estrogen-treated immature rats in vivo.

    Gene expression databases

    GenevestigatoriP14137.

    Interactioni

    Subunit structurei

    Interacts with FDX1/adrenodoxin.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP14137.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117408.
    HOGENOMiHOG000013161.
    HOVERGENiHBG051098.
    InParanoidiP14137.
    KOiK00498.
    OMAiYQRPIGV.
    OrthoDBiEOG7MKW5S.
    PhylomeDBiP14137.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14137-1 [UniParc]FASTAAdd to Basket

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    MLAKGLCLRS VLVKSCQPFL SPVWQGPGLA TGNGAGISST NSPRSFNEIP    50
    SPGDNGWINL YHFLRENGTH RIHYHHMQNF QKYGPIYREK LGNMESVYIL 100
    DPKDAATLFS CEGPNPERYL VPPWVAYHQY YQRPIGVLFK SSDAWRKDRI 150
    VLNQEVMAPD SIKNFVPLLE GVAQDFIKVL HRRIKQQNSG KFSGDISDDL 200
    FRFAFESITS VVFGERLGML EEIVDPESQR FIDAVYQMFH TSVPMLNMPP 250
    DLFRLFRTKT WKDHAAAWDV IFSKADEYTQ NFYWDLRQKR DFSKYPGVLY 300
    SLLGGNKLPF KNIQANITEM LAGGVDTTSM TLQWNLYEMA HNLKVQEMLR 350
    AEVLAARRQA QGDMAKMVQL VPLLKASIKE TLRLHPISVT LQRYIVNDLV 400
    LRNYKIPAKT LVQVASYAMG RESSFFPNPN KFDPTRWLEK SQNTTHFRYL 450
    GFGWGVRQCL GRRIAELEMT IFLINVLENF RIEVQSIRDV GTKFNLILMP 500
    EKPIFFNFQP LKQDLGSTMP RKGDTV 526
    Length:526
    Mass (Da):60,586
    Last modified:January 1, 1990 - v1
    Checksum:iB2930E40018DC1EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05156 mRNA. Translation: AAA40989.1.
    M63133
    , M63125, M63126, M63127, M63128, M63129, M63130, M63131, M63132 Genomic DNA. Translation: AAA40958.1.
    BC089100 mRNA. Translation: AAH89100.1.
    M22615 mRNA. Translation: AAA62267.1.
    PIRiA34164.
    RefSeqiNP_058982.1. NM_017286.2.
    UniGeneiRn.1401.

    Genome annotation databases

    EnsembliENSRNOT00000010831; ENSRNOP00000010831; ENSRNOG00000008074.
    GeneIDi29680.
    KEGGirno:29680.
    UCSCiRGD:69325. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05156 mRNA. Translation: AAA40989.1 .
    M63133
    , M63125 , M63126 , M63127 , M63128 , M63129 , M63130 , M63131 , M63132 Genomic DNA. Translation: AAA40958.1 .
    BC089100 mRNA. Translation: AAH89100.1 .
    M22615 mRNA. Translation: AAA62267.1 .
    PIRi A34164.
    RefSeqi NP_058982.1. NM_017286.2.
    UniGenei Rn.1401.

    3D structure databases

    ProteinModelPortali P14137.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P14137.
    ChEMBLi CHEMBL5246.

    Proteomic databases

    PRIDEi P14137.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010831 ; ENSRNOP00000010831 ; ENSRNOG00000008074 .
    GeneIDi 29680.
    KEGGi rno:29680.
    UCSCi RGD:69325. rat.

    Organism-specific databases

    CTDi 1583.
    RGDi 69325. Cyp11a1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117408.
    HOGENOMi HOG000013161.
    HOVERGENi HBG051098.
    InParanoidi P14137.
    KOi K00498.
    OMAi YQRPIGV.
    OrthoDBi EOG7MKW5S.
    PhylomeDBi P14137.

    Enzyme and pathway databases

    UniPathwayi UPA00229 .
    SABIO-RK P14137.

    Miscellaneous databases

    NextBioi 610031.
    PROi P14137.

    Gene expression databases

    Genevestigatori P14137.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. cDNA and deduced amino acid sequence of rat P-450scc."
      Oonk R.B., Krasnow J.S., Beattie W.G., Richards J.S.
      J. Biol. Chem. 264:21934-21942(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Rat cholesterol side-chain cleavage cytochrome P-450 (P-450scc) gene. Structure and regulation by cAMP in vitro."
      Oonk R.B., Parker K.L., Gibson J.L., Richards J.S.
      J. Biol. Chem. 265:22392-22401(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    4. "Rat cholesterol side-chain cleavage enzyme (P-450scc): use of a cDNA probe to study the hormonal regulation of P-450scc mRNA levels in ovarian granulosa cells."
      McMasters K.M., Dickson L.A., Shamy R.V., Robischon K., Macdonald G.J., Moyle W.R.
      Gene 57:1-9(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 344-526, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Ovary.

    Entry informationi

    Entry nameiCP11A_RAT
    AccessioniPrimary (citable) accession number: P14137
    Secondary accession number(s): Q5FWY8, Q6LDR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3