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P14137 (CP11A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholesterol side-chain cleavage enzyme, mitochondrial

EC=1.14.15.6
Alternative name(s):
CYPXIA1
Cholesterol desmolase
Cytochrome P450 11A1
Cytochrome P450(scc)
Gene names
Name:Cyp11a1
Synonyms:Cyp11a, Cyp11a-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Catalytic activity

Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; C21-steroid hormone metabolism.

Subunit structure

Interacts with FDX1/adrenodoxin By similarity.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Expressed in ovary, adrenal and testis. Ref.4

Induction

Induced by FSH or pregnant mare's serum gonadotropin in ovaries of estrogen-treated immature rats in vivo.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processCholesterol metabolism
Lipid metabolism
Steroid metabolism
Steroidogenesis
Sterol metabolism
   Cellular componentMembrane
Mitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processC21-steroid hormone biosynthetic process

Inferred from direct assay PubMed 19665544. Source: RGD

Leydig cell differentiation

Inferred from expression pattern PubMed 18502897. Source: RGD

Schwann cell differentiation

Inferred from expression pattern PubMed 18373277. Source: RGD

biphenyl metabolic process

Inferred from expression pattern PubMed 19071209PubMed 20356859. Source: RGD

cellular response to antibiotic

Inferred from expression pattern PubMed 18655822. Source: RGD

cellular response to cAMP

Inferred from expression pattern PubMed 17719163. Source: RGD

cellular response to cadmium ion

Inferred from expression pattern PubMed 17719163. Source: RGD

cellular response to fibroblast growth factor stimulus

Inferred from expression pattern PubMed 17494997. Source: RGD

cellular response to follicle-stimulating hormone stimulus

Inferred from expression pattern PubMed 20810564. Source: RGD

cellular response to gonadotropin stimulus

Inferred from expression pattern PubMed 9397943. Source: RGD

cellular response to interleukin-1

Inferred from expression pattern PubMed 9397943PubMed 9582502. Source: RGD

cellular response to lipopolysaccharide

Inferred from expression pattern PubMed 20665543. Source: RGD

cellular response to peptide hormone stimulus

Inferred from expression pattern PubMed 19416745. Source: RGD

cellular response to transforming growth factor beta stimulus

Inferred from expression pattern PubMed 18511507. Source: RGD

cellular response to tumor necrosis factor

Inferred from expression pattern PubMed 9397943. Source: RGD

cerebellum development

Inferred from expression pattern PubMed 16329132. Source: RGD

cholesterol metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

dibenzo-p-dioxin metabolic process

Inferred from expression pattern PubMed 18637154. Source: RGD

estrogen biosynthetic process

Inferred from expression pattern PubMed 20810564. Source: RGD

fractalkine metabolic process

Inferred from expression pattern PubMed 18292196. Source: RGD

granulosa cell differentiation

Inferred from expression pattern Ref.2. Source: RGD

hippocampus development

Inferred from expression pattern PubMed 21047951. Source: RGD

male gonad development

Inferred from expression pattern PubMed 18772241. Source: RGD

maternal process involved in female pregnancy

Inferred from expression pattern PubMed 17726144. Source: RGD

mating behavior

Inferred from mutant phenotype PubMed 16101892. Source: RGD

organic acid metabolic process

Inferred from expression pattern PubMed 17400581. Source: RGD

phenol-containing compound metabolic process

Inferred from expression pattern PubMed 18191889. Source: RGD

phthalate metabolic process

Inferred from expression pattern PubMed 18923996. Source: RGD

progesterone biosynthetic process

Inferred from expression pattern PubMed 20810564. Source: RGD

response to L-ascorbic acid

Inferred from expression pattern PubMed 17280759. Source: RGD

response to acid

Inferred from expression pattern PubMed 18040670. Source: RGD

response to alkaloid

Inferred from expression pattern PubMed 18772241. Source: RGD

response to antibiotic

Inferred from expression pattern PubMed 19118620. Source: RGD

response to cAMP

Inferred from expression pattern Ref.2. Source: RGD

response to cadmium ion

Inferred from expression pattern PubMed 15223132. Source: RGD

response to corticosterone

Inferred from expression pattern PubMed 18335507. Source: RGD

response to drug

Inferred from expression pattern PubMed 20144211. Source: RGD

response to estrogen

Inferred from expression pattern PubMed 12715917PubMed 19734697. Source: RGD

response to fungicide

Inferred from expression pattern PubMed 14967917. Source: RGD

response to gamma radiation

Inferred from expression pattern PubMed 18046538. Source: RGD

response to genistein

Inferred from expression pattern PubMed 16214947. Source: RGD

response to gonadotropin

Inferred from expression pattern PubMed 19929576. Source: RGD

response to hydrogen peroxide

Inferred from mutant phenotype PubMed 14635199. Source: RGD

response to insecticide

Inferred from expression pattern PubMed 19892000. Source: RGD

response to ionizing radiation

Inferred from expression pattern PubMed 18821396. Source: RGD

response to nutrient

Inferred from expression pattern PubMed 16844298. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 18606229. Source: RGD

response to organic substance

Inferred from expression pattern PubMed 16226009PubMed 18804513. Source: RGD

response to peptide hormone

Inferred from expression pattern PubMed 18609294. Source: RGD

response to salt stress

Inferred from expression pattern PubMed 7678819. Source: RGD

response to steroid hormone

Inferred from expression pattern PubMed 19961867. Source: RGD

response to vitamin E

Inferred from expression pattern PubMed 17280759. Source: RGD

steroid biosynthetic process

Inferred from mutant phenotype PubMed 16226009PubMed 17218406. Source: RGD

testosterone biosynthetic process

Inferred from expression pattern PubMed 19883722. Source: RGD

   Cellular_componentmitochondrial crista

Inferred from direct assay PubMed 11691658. Source: RGD

mitochondrial inner membrane

Inferred from direct assay PubMed 21075169. Source: RGD

mitochondrion

Inferred from direct assay PubMed 8674848. Source: RGD

perikaryon

Inferred from direct assay PubMed 16632873. Source: RGD

   Molecular_functioncholesterol binding

Inferred from mutant phenotype PubMed 16226009. Source: RGD

cholesterol monooxygenase (side-chain-cleaving) activity

Inferred from mutant phenotype PubMed 16226009. Source: RGD

heme binding

Inferred from sequence or structural similarity. Source: UniProtKB

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Mitochondrion
Chain37 – 526490Cholesterol side-chain cleavage enzyme, mitochondrial
PRO_0000003590

Sites

Metal binding4591Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P14137 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: B2930E40018DC1EE

FASTA52660,586
        10         20         30         40         50         60 
MLAKGLCLRS VLVKSCQPFL SPVWQGPGLA TGNGAGISST NSPRSFNEIP SPGDNGWINL 

        70         80         90        100        110        120 
YHFLRENGTH RIHYHHMQNF QKYGPIYREK LGNMESVYIL DPKDAATLFS CEGPNPERYL 

       130        140        150        160        170        180 
VPPWVAYHQY YQRPIGVLFK SSDAWRKDRI VLNQEVMAPD SIKNFVPLLE GVAQDFIKVL 

       190        200        210        220        230        240 
HRRIKQQNSG KFSGDISDDL FRFAFESITS VVFGERLGML EEIVDPESQR FIDAVYQMFH 

       250        260        270        280        290        300 
TSVPMLNMPP DLFRLFRTKT WKDHAAAWDV IFSKADEYTQ NFYWDLRQKR DFSKYPGVLY 

       310        320        330        340        350        360 
SLLGGNKLPF KNIQANITEM LAGGVDTTSM TLQWNLYEMA HNLKVQEMLR AEVLAARRQA 

       370        380        390        400        410        420 
QGDMAKMVQL VPLLKASIKE TLRLHPISVT LQRYIVNDLV LRNYKIPAKT LVQVASYAMG 

       430        440        450        460        470        480 
RESSFFPNPN KFDPTRWLEK SQNTTHFRYL GFGWGVRQCL GRRIAELEMT IFLINVLENF 

       490        500        510        520 
RIEVQSIRDV GTKFNLILMP EKPIFFNFQP LKQDLGSTMP RKGDTV 

« Hide

References

« Hide 'large scale' references
[1]"Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. cDNA and deduced amino acid sequence of rat P-450scc."
Oonk R.B., Krasnow J.S., Beattie W.G., Richards J.S.
J. Biol. Chem. 264:21934-21942(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Rat cholesterol side-chain cleavage cytochrome P-450 (P-450scc) gene. Structure and regulation by cAMP in vitro."
Oonk R.B., Parker K.L., Gibson J.L., Richards J.S.
J. Biol. Chem. 265:22392-22401(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[4]"Rat cholesterol side-chain cleavage enzyme (P-450scc): use of a cDNA probe to study the hormonal regulation of P-450scc mRNA levels in ovarian granulosa cells."
McMasters K.M., Dickson L.A., Shamy R.V., Robischon K., Macdonald G.J., Moyle W.R.
Gene 57:1-9(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 344-526, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05156 mRNA. Translation: AAA40989.1.
M63133 expand/collapse EMBL AC list , M63125, M63126, M63127, M63128, M63129, M63130, M63131, M63132 Genomic DNA. Translation: AAA40958.1.
BC089100 mRNA. Translation: AAH89100.1.
M22615 mRNA. Translation: AAA62267.1.
PIRA34164.
RefSeqNP_058982.1. NM_017286.2.
UniGeneRn.1401.

3D structure databases

ProteinModelPortalP14137.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP14137.
ChEMBLCHEMBL5246.

Proteomic databases

PRIDEP14137.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010831; ENSRNOP00000010831; ENSRNOG00000008074.
GeneID29680.
KEGGrno:29680.
UCSCRGD:69325. rat.

Organism-specific databases

CTD1583.
RGD69325. Cyp11a1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117408.
HOGENOMHOG000013161.
HOVERGENHBG051098.
InParanoidP14137.
KOK00498.
OMAYQRPIGV.
OrthoDBEOG7MKW5S.
PhylomeDBP14137.

Enzyme and pathway databases

SABIO-RKP14137.
UniPathwayUPA00229.

Gene expression databases

GenevestigatorP14137.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610031.
PROP14137.

Entry information

Entry nameCP11A_RAT
AccessionPrimary (citable) accession number: P14137
Secondary accession number(s): Q5FWY8, Q6LDR9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways