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Reviewed, UniProtKB/Swiss-Prot P14137 (CP11A_RAT)

Last modified October 13, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    Cytochrome P450 11A1
    CYPXIA1
    P450(scc)
    Cholesterol desmolase
Gene names
Name: Cyp11a1
Synonyms: Cyp11a, Cyp11a-1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Catalytic activity

Cholesterol + reduced adrenal ferredoxin + O2 = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H2O.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; C21-steroid hormone metabolism.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Expressed in ovary, adrenal and testis. Ref.4

Induction

Induced by FSH or pregnant mare's serum gonadotropin in ovaries of estrogen-treated immature rats in vivo.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Mitochondrion
Chain37 – 526490Cholesterol side-chain cleavage enzyme, mitochondrial
PRO_0000003590

Sites

Metal binding4591Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P14137-1 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: B2930E40018DC1EE

FASTA52660,586
        10         20         30         40         50         60 
MLAKGLCLRS VLVKSCQPFL SPVWQGPGLA TGNGAGISST NSPRSFNEIP SPGDNGWINL 

        70         80         90        100        110        120 
YHFLRENGTH RIHYHHMQNF QKYGPIYREK LGNMESVYIL DPKDAATLFS CEGPNPERYL 

       130        140        150        160        170        180 
VPPWVAYHQY YQRPIGVLFK SSDAWRKDRI VLNQEVMAPD SIKNFVPLLE GVAQDFIKVL 

       190        200        210        220        230        240 
HRRIKQQNSG KFSGDISDDL FRFAFESITS VVFGERLGML EEIVDPESQR FIDAVYQMFH 

       250        260        270        280        290        300 
TSVPMLNMPP DLFRLFRTKT WKDHAAAWDV IFSKADEYTQ NFYWDLRQKR DFSKYPGVLY 

       310        320        330        340        350        360 
SLLGGNKLPF KNIQANITEM LAGGVDTTSM TLQWNLYEMA HNLKVQEMLR AEVLAARRQA 

       370        380        390        400        410        420 
QGDMAKMVQL VPLLKASIKE TLRLHPISVT LQRYIVNDLV LRNYKIPAKT LVQVASYAMG 

       430        440        450        460        470        480 
RESSFFPNPN KFDPTRWLEK SQNTTHFRYL GFGWGVRQCL GRRIAELEMT IFLINVLENF 

       490        500        510        520 
RIEVQSIRDV GTKFNLILMP EKPIFFNFQP LKQDLGSTMP RKGDTV 

« Hide

References

« Hide 'large scale' references
[1]"Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. cDNA and deduced amino acid sequence of rat P-450scc."
Oonk R.B., Krasnow J.S., Beattie W.G., Richards J.S.
J. Biol. Chem. 264:21934-21942(1989) [PubMed: 2480959] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Rat cholesterol side-chain cleavage cytochrome P-450 (P-450scc) gene. Structure and regulation by cAMP in vitro."
Oonk R.B., Parker K.L., Gibson J.L., Richards J.S.
J. Biol. Chem. 265:22392-22401(1990) [PubMed: 2176216] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[4]"Rat cholesterol side-chain cleavage enzyme (P-450scc): use of a cDNA probe to study the hormonal regulation of P-450scc mRNA levels in ovarian granulosa cells."
McMasters K.M., Dickson L.A., Shamy R.V., Robischon K., Macdonald G.J., Moyle W.R.
Gene 57:1-9(1987) [PubMed: 3123325] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 344-526, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

J05156 mRNA. Translation: AAA40989.1.
M63133 expand/collapse EMBL AC list , M63125, M63126, M63127, M63128, M63129, M63130, M63131, M63132 Genomic DNA. Translation: AAA40958.1.
BC089100 mRNA. Translation: AAH89100.1.
M22615 mRNA. Translation: AAA62267.1.
IPIIPI00325713.
PIRA34164.
RefSeqNP_058982.1.
UniGeneRn.1401

3D structure databases

HSSPHSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBaseSearch...

Protein-protein interaction databases

STRINGP14137.

Genome annotation databases

EnsemblENSRNOT00000010831; ENSRNOP00000010831; ENSRNOG00000008074; Rattus norvegicus. [Genome view]
GeneID29680.
KEGGrno:29680.
UCSCNM_017286. rat.

Organism-specific databases

CTD29680.
RGD69325. Cyp11a1.

Phylogenomic databases

HOVERGENP14137.

Enzyme and pathway databases

BRENDA1.14.15.6. 248.

Gene expression databases

ArrayExpressP14137.
GenevestigatorP14137.
GermOnlineENSRNOG00000008074. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio610031.

Entry information

Entry nameCP11A_RAT
AccessionPrimary (citable) accession number: P14137
Secondary accession number(s): Q5FWY8, Q6LDR9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 13, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents