60S ribosomal protein L3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P14126 (RL3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 136. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L3

Alternative name(s):
Maintenance of killer protein 8
RP1
Trichodermin resistance protein
YL1

Gene names

Name:	RPL3
Synonyms:	MAK8, TCM1
Ordered Locus Names:	YOR063W
ORF Names:	YOR29-14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm Ref.9.
Disruption phenotype	A mutant confers resistance to trichodermin, a trichotecene toxin produced by plant-pathogenic fungi. Ref.1
Miscellaneous	Present with 450 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein L3P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Methylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.7. Source: SGD ribosomal large subunit assembly Inferred from mutant phenotype PubMed 3537704. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.7. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRV2	P17555	3	EBI-15364,EBI-4024

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.6

Chain

2 – 387

386

60S ribosomal protein L3

PRO_0000077251

Amino acid modifications

Modified residue

Phosphoserine Ref.8 Ref.12

Modified residue

Phosphothreonine Ref.14

Modified residue

103

Phosphothreonine Ref.11

Modified residue

156

Phosphoserine Ref.12

Modified residue

164

Phosphothreonine Ref.12

Modified residue

243

Pros-methylhistidine Ref.15

Modified residue

297

Phosphoserine Ref.12 Ref.13 Ref.14

Modified residue

355

Phosphoserine Ref.13

Experimental info

Sequence conflict

255

W → C in AAA88732. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P14126 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 986769A218E9698A
Show »

FASTA

387

43,758

        10         20         30         40         50         60 
MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL 

        70         80         90        100        110        120 
DRPGSKFHKR EVVEAVTVVD TPPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK 

       130        140        150        160        170        180 
NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV LVHTQIRKTP LAQKKAHLAE 

       190        200        210        220        230        240 
IQLNGGSISE KVDWAREHFE KTVAVDSVFE QNEMIDAIAV TKGHGFEGVT HRWGTKKLPR 

       250        260        270        280        290        300 
KTHRGLRKVA CIGAWHPAHV MWSVARAGQR GYHSRTSINH KIYRVGKGDD EANGATSFDR 

       310        320        330        340        350        360 
TKKTITPMGG FVHYGEIKND FIMVKGCIPG NRKRIVTLRK SLYTNTSRKA LEEVSLKWID 

       370        380 
TASKFGKGRF QTPAEKHAFM GTLKKDL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the tcm1 gene (ribosomal protein L3) of Saccharomyces cerevisiae." Schultz L.D., Friesen J.D. J. Bacteriol. 155:8-14(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: CLP1.
[2]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 191:519-524(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-10.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, MASS SPECTROMETRY. Strain: 2124.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-103, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-156; THR-164 AND SER-297, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297 AND SER-355, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-95 AND SER-297, MASS SPECTROMETRY.
[15]	"A novel 3-methylhistidine modification of yeast ribosomal protein Rpl3 is dependent upon the YIL110W methyltransferase." Webb K.J., Zurita-Lopez C.I., Al-Hadid Q., Laganowsky A., Young B.D., Lipson R.S., Souda P., Faull K.F., Whitelegge J.P., Clarke S.G. J. Biol. Chem. 285:37598-37606(2010) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT HIS-243.
[16]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 8-366, ELECTRON MICROSCOPY.
[17]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01351 Genomic DNA. Translation: AAA88732.1.
Z74971 Genomic DNA. Translation: CAA99256.1.
Z70678 Genomic DNA. Translation: CAA94548.1.
BK006948 Genomic DNA. Translation: DAA10842.1.

PIR

R5BY4E. S66946.

RefSeq

NP_014706.1. NM_001183482.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	C	8-366	[»]
1S1I	electron microscopy	11.70	C	2-387	[»]
3IZS	electron microscopy	-	C	1-387	[»]
3O58	X-ray	4.00	C	1-387	[»]
3O5H	X-ray	4.00	C	1-387	[»]
3U5E	X-ray	3.00	B	1-387	[»]
3U5I	X-ray	3.00	B	1-387	[»]
4B6A	electron microscopy	8.10	B	1-387	[»]

ProteinModelPortal

P14126.

SMR

P14126. Positions 2-387.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6264N.

IntAct

P14126. 99 interactions.

MINT

MINT-604187.

STRING

4932.YOR063W.

Proteomic databases

PaxDb

P14126.

PeptideAtlas

P14126.

PRIDE

P14126.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOR063W; YOR063W; YOR063W.

GeneID

854229.

KEGG

sce:YOR063W.

Organism-specific databases

SGD

S000005589. RPL3.

Phylogenomic databases

eggNOG

COG0087.

GeneTree

ENSGT00390000017606.

HOGENOM

HOG000107319.

K02925.

OMA

GFHQRTE.

OrthoDB

EOG4CG3HR.

Enzyme and pathway databases

BioCyc

YEAST:G3O-33603-MONOMER.

Gene expression databases

Genevestigator

P14126.

GermOnline

YOR063W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000597. Ribosomal_L3.
IPR019926. Ribosomal_L3_CS.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]

Pfam

PF00297. Ribosomal_L3. 1 hit.
[Graphical view]

SUPFAM

SSF50447. Translat_factor. 1 hit.

PROSITE

PS00474. RIBOSOMAL_L3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14126.

NextBio

976109.

Entry information

Entry name

RL3_YEAST

Accession

Primary (citable) accession number: P14126
Secondary accession number(s): D6W2C6, Q08459

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 136 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents