50S ribosomal protein L18P - Haloarcula marismortui

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Reviewed, UniProtKB/Swiss-Prot P14123 (RL18_HALMA)

Last modified January 19, 2010. Version 86. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L18P
Alternative name(s):
    Hmal18
    Hl12

Gene names

Name:	rpl18p
Ordered Locus Names:	rrnAC1593

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of 5 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit, where it forms part of the central protuberance and stabilizes the orientation of adjacent RNA domains. HAMAP MF_01337
Subunit structure	Part of the 50S ribosomal subunit. Interacts with proteins L5 and L21e, and attaches the 5S rRNA to the 23S rRNA. Has been cross-linked to L21e. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the ribosomal protein L18P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5S rRNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed Ref.3

Chain

186

50S ribosomal protein L18P HAMAP MF_01337

PRO_0000131400

Experimental info

Sequence conflict

1

R → A AA sequence Ref.3

Secondary structure

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187

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

P14123-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 60E9AF3CB64E7CA5
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187

20,614

        10         20         30         40         50         60 
MATGPRYKVP MRRRREARTD YHQRLRLLKS GKPRLVARKS NKHVRAQLVT LGPNGDDTLA 

        70         80         90        100        110        120 
SAHSSDLAEY GWEAPTGNMP SAYLTGLLAG LRAQEAGVEE AVLDIGLNSP TPGSKVFAIQ 

       130        140        150        160        170        180 
EGAIDAGLDI PHNDDVLADW QRTRGAHIAE YDEQLEEPLY SGDFDAADLP EHFDELRETL 


LDGDIEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and nucleotide sequence of ten ribosomal protein genes from the region equivalent to the spectinomycin operon in the archaebacterium Halobacterium marismortui." Scholzen T., Arndt E. Mol. Gen. Genet. 228:70-80(1991) [PubMed: 1832208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[3]	"Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane." Walsh M.J., McDougall J., Wittmann-Liebold B. Biochemistry 27:6867-6876(1988) [PubMed: 3196689] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-25.
[4]	"Localization of proteins HL29 and HL31 from Haloarcula marismortui within the 50 S ribosomal subunit by chemical crosslinking." Bergmann U., Wittmann-Liebold B. J. Mol. Biol. 232:693-700(1993) [PubMed: 8345527] [Abstract] Cited for: CROSS-LINKING TO L21E.
[5]	"The complete atomic structure of the large ribosomal subunit at 2.4 A resolution." Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A. Science 289:905-920(2000) [PubMed: 10937989] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[6]	"The structural basis of ribosome activity in peptide bond synthesis." Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A. Science 289:920-930(2000) [PubMed: 10937990] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[7]	"A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits." Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A. Nat. Struct. Biol. 9:225-230(2002) [PubMed: 11828326] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[8]	"The kink-turn: a new RNA secondary structure motif." Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A. EMBO J. 20:4214-4221(2001) [PubMed: 11483524] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[9]	"The structures of four macrolide antibiotics bound to the large ribosomal subunit." Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A. Mol. Cell 10:117-128(2002) [PubMed: 12150912] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[10]	"Structural insights into peptide bond formation." Hansen J.L., Schmeing T.M., Moore P.B., Steitz T.A. Proc. Natl. Acad. Sci. U.S.A. 99:11670-11675(2002) [PubMed: 12185246] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[11]	"Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit." Hansen J.L., Moore P.B., Steitz T.A. J. Mol. Biol. 330:1061-1075(2003) [PubMed: 12860128] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[12]	"Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit." Schmeing T.M., Moore P.B., Steitz T.A. RNA 9:1345-1352(2003) [PubMed: 14561884] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X58395 Genomic DNA. Translation: CAA41290.1.
AY596297 Genomic DNA. Translation: AAV46511.1.

PIR

R5HS18. S16541.

RefSeq

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FFK	X-ray	2.40	K	2-187	[»]
1JJ2	X-ray	2.40	M	2-187	[»]
1K73	X-ray	3.01	O	2-187	[»]
1K8A	X-ray	3.00	O	2-187	[»]
1K9M	X-ray	3.00	O	2-187	[»]
1KC8	X-ray	3.01	O	2-187	[»]
1KD1	X-ray	3.00	O	2-187	[»]
1KQS	X-ray	3.10	M	2-187	[»]
1M1K	X-ray	3.20	O	2-187	[»]
1M90	X-ray	2.80	O	2-187	[»]
1N8R	X-ray	3.00	O	2-187	[»]
1NJI	X-ray	3.00	O	2-187	[»]
1Q7Y	X-ray	3.20	O	2-187	[»]
1Q81	X-ray	2.95	O	2-187	[»]
1Q82	X-ray	2.98	O	2-187	[»]
1Q86	X-ray	3.00	O	2-187	[»]
1QVF	X-ray	3.10	M	2-187	[»]
1QVG	X-ray	2.90	M	2-187	[»]
1S72	X-ray	2.40	N	1-187	[»]
1VQ4	X-ray	2.70	N	1-187	[»]
1VQ5	X-ray	2.60	N	1-187	[»]
1VQ6	X-ray	2.70	N	1-187	[»]
1VQ7	X-ray	2.50	N	1-187	[»]
1VQ8	X-ray	2.20	N	1-187	[»]
1VQ9	X-ray	2.40	N	1-187	[»]
1VQK	X-ray	2.30	N	1-187	[»]
1VQL	X-ray	2.30	N	1-187	[»]
1VQM	X-ray	2.30	N	1-187	[»]
1VQN	X-ray	2.40	N	1-187	[»]
1VQO	X-ray	2.20	N	1-187	[»]
1VQP	X-ray	2.25	N	1-187	[»]
1W2B	X-ray	3.50	M	2-186	[»]
1YHQ	X-ray	2.40	N	1-187	[»]
1YI2	X-ray	2.65	N	1-187	[»]
1YIJ	X-ray	2.60	N	1-187	[»]
1YIT	X-ray	2.80	N	1-187	[»]
1YJ9	X-ray	2.90	N	1-187	[»]
1YJN	X-ray	3.00	N	1-187	[»]
1YJW	X-ray	2.90	N	1-187	[»]
2B66	X-ray	5.90	S	2-187	[»]
2B9N	X-ray	6.76	S	2-187	[»]
2B9P	X-ray	6.46	S	2-187	[»]
2OTJ	X-ray	2.90	N	1-187	[»]
2OTL	X-ray	2.70	N	1-187	[»]
2QA4	X-ray	3.00	N	1-187	[»]
2QEX	X-ray	2.90	N	1-187	[»]
3CC2	X-ray	2.40	N	1-187	[»]
3CC4	X-ray	2.70	N	1-187	[»]
3CC7	X-ray	2.70	N	1-187	[»]
3CCE	X-ray	2.75	N	1-187	[»]
3CCJ	X-ray	2.70	N	1-187	[»]
3CCL	X-ray	2.90	N	1-187	[»]
3CCM	X-ray	2.55	N	1-187	[»]
3CCQ	X-ray	2.90	N	1-187	[»]
3CCR	X-ray	3.00	N	1-187	[»]
3CCS	X-ray	2.95	N	1-187	[»]
3CCU	X-ray	2.80	N	1-187	[»]
3CCV	X-ray	2.90	N	1-187	[»]
3CD6	X-ray	2.75	N	1-187	[»]
3CMA	X-ray	2.80	N	1-187	[»]
3CME	X-ray	2.95	N	1-187	[»]
3CPW	X-ray	2.70	M	1-187	[»]
3CXC	X-ray	3.00	M	2-186	[»]
3G4S	X-ray	3.20	N	2-187	[»]
3G6E	X-ray	2.70	N	2-187	[»]
3G71	X-ray	2.85	N	2-187	[»]

ModBase

Genome annotation databases

GeneID

GenomeReviews

Gene locus rrnAC1593 in contig AY596297_GR.

KEGG

NMPDR

fig|272569.1.peg.1484.

Organism-specific databases

CMR

Phylogenomic databases

HOGENOM

OMA

Enzyme and pathway databases

BioCyc

HMAR272569:RRNAC1593-MONOMER.

Family and domain databases

HAMAP

MF_01337_A. Ribosomal_L18_A.
[Tree]

InterPro

IPR005484. Ribosomal_L18/L5.
IPR005485. Ribosomal_L5_euk.
[Graphical view]

PANTHER

PTHR23410. Ribosomal_L5euk. 1 hit.

Pfam

PF00861. Ribosomal_L18p. 1 hit.
[Graphical view]

PRINTS

PR00058. RIBOSOMALL5.

ProtoNet

Entry information

Entry name

RL18_HALMA

Accession

Primary (citable) accession number: P14123
Secondary accession number(s): Q5V1U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents