Reviewed, UniProtKB/Swiss-Prot P14121 (RL30_HALMA)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L30P
Alternative name(s):
    Hmal30
    Hl20
    Hl16

Gene names

Name:	rpl30p
Ordered Locus Names:	rrnAC1591

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of 5 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit, stabilizing the orientation of adjacent RNA domains. HAMAP MF_01371
Subunit structure	Part of the 50S ribosomal subunit. Binds 5S rRNA. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the ribosomal protein L30P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 154

154

50S ribosomal protein L30P HAMAP MF_01371

PRO_0000104622

Experimental info

Sequence conflict

83

1

W → L Ref.2

Sequence conflict

148

1

Missing Ref.2

Secondary structure

1

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154

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

15 – 23

9

Beta strand

31 – 35

5

Helix

39 – 48

10

Helix

49 – 51

3

Beta strand

52 – 55

4

Helix

59 – 69

11

Beta strand

72 – 76

5

Helix

81 – 87

7

Beta strand

88 – 92

5

Helix

93 – 101

9

Turn

107 – 111

5

Beta strand

128 – 131

4

Helix

132 – 134

3

Beta strand

136 – 140

5

Helix

143 – 152

10

Sequences

	Sequence		Length	Mass (Da)
	P14121-1 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: ED673F036E974C14	FASTA	154	17,042
	10 20 30 40 50 60 MHALVQLRGE VNMHTDIQDT LEMLNIHHVN HCTLVPETDA YRGMVAKVND FVAFGEPSQE 70 80 90 100 110 120 TLETVLATRA EPLEGDADVD DEWVAEHTDY DDISGLAFAL LSEETTLREQ GLSPTLRLHP 130 140 150 PRGGHDGVKH PVKEGGQLGK HDTEGIDDLL EAMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structures of five ribosomal proteins from the archaebacterium Halobacterium marismortui and their structural relationships to eubacterial and eukaryotic ribosomal proteins." Hatakeyama T., Kaufmann F., Schroeter B., Hatakeyama T. Eur. J. Biochem. 185:685-693(1989) [PubMed: 2591382] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Organization and nucleotide sequence of ten ribosomal protein genes from the region equivalent to the spectinomycin operon in the archaebacterium Halobacterium marismortui." Scholzen T., Arndt E. Mol. Gen. Genet. 228:70-80(1991) [PubMed: 1832208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[4]	"Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane." Walsh M.J., McDougall J., Wittmann-Liebold B. Biochemistry 27:6867-6876(1988) [PubMed: 3196689] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30.
[5]	"The complete atomic structure of the large ribosomal subunit at 2.4 A resolution." Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A. Science 289:905-920(2000) [PubMed: 10937989] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[6]	"The structural basis of ribosome activity in peptide bond synthesis." Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A. Science 289:920-930(2000) [PubMed: 10937990] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[7]	"A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits." Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A. Nat. Struct. Biol. 9:225-230(2002) [PubMed: 11828326] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[8]	"The kink-turn: a new RNA secondary structure motif." Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A. EMBO J. 20:4214-4221(2001) [PubMed: 11483524] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[9]	"The structures of four macrolide antibiotics bound to the large ribosomal subunit." Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A. Mol. Cell 10:117-128(2002) [PubMed: 12150912] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[10]	"Structural insights into peptide bond formation." Hansen J.L., Schmeing T.M., Moore P.B., Steitz T.A. Proc. Natl. Acad. Sci. U.S.A. 99:11670-11675(2002) [PubMed: 12185246] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[11]	"Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit." Hansen J.L., Moore P.B., Steitz T.A. J. Mol. Biol. 330:1061-1075(2003) [PubMed: 12860128] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[12]	"Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit." Schmeing T.M., Moore P.B., Steitz T.A. RNA 9:1345-1352(2003) [PubMed: 14561884] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X58395 Genomic DNA. Translation: CAA41292.1.
AY596297 Genomic DNA. Translation: AAV46509.1.

PIR

R5HS30. S16543.

RefSeq

YP_136215.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FFK	X-ray	2.40	T	1-154	[»]
1JJ2	X-ray	2.40	V	1-154	[»]
1K73	X-ray	3.01	X	1-154	[»]
1K8A	X-ray	3.00	X	1-154	[»]
1K9M	X-ray	3.00	X	1-154	[»]
1KC8	X-ray	3.01	X	1-154	[»]
1KD1	X-ray	3.00	X	1-154	[»]
1KQS	X-ray	3.10	V	1-154	[»]
1M1K	X-ray	3.20	X	1-154	[»]
1M90	X-ray	2.80	X	1-154	[»]
1N8R	X-ray	3.00	X	1-154	[»]
1NJI	X-ray	3.00	X	1-154	[»]
1Q7Y	X-ray	3.20	X	1-154	[»]
1Q81	X-ray	2.95	X	1-154	[»]
1Q82	X-ray	2.98	X	1-154	[»]
1Q86	X-ray	3.00	X	1-154	[»]
1QVF	X-ray	3.10	V	1-154	[»]
1QVG	X-ray	2.90	V	1-154	[»]
1S72	X-ray	2.40	W	1-154	[»]
1VQ4	X-ray	2.70	W	1-154	[»]
1VQ5	X-ray	2.60	W	1-154	[»]
1VQ6	X-ray	2.70	W	1-154	[»]
1VQ7	X-ray	2.50	W	1-154	[»]
1VQ8	X-ray	2.20	W	1-154	[»]
1VQ9	X-ray	2.40	W	1-154	[»]
1VQK	X-ray	2.30	W	1-154	[»]
1VQL	X-ray	2.30	W	1-154	[»]
1VQM	X-ray	2.30	W	1-154	[»]
1VQN	X-ray	2.40	W	1-154	[»]
1VQO	X-ray	2.20	W	1-154	[»]
1VQP	X-ray	2.25	W	1-154	[»]
1W2B	X-ray	3.50	V	1-154	[»]
1YHQ	X-ray	2.40	W	1-154	[»]
1YI2	X-ray	2.65	W	1-154	[»]
1YIJ	X-ray	2.60	W	1-154	[»]
1YIT	X-ray	2.80	W	1-154	[»]
1YJ9	X-ray	2.90	W	1-154	[»]
1YJN	X-ray	3.00	W	1-154	[»]
1YJW	X-ray	2.90	W	1-154	[»]
2OTJ	X-ray	2.90	W	1-154	[»]
2OTL	X-ray	2.70	W	1-154	[»]
2QA4	X-ray	3.00	W	1-154	[»]
2QEX	X-ray	2.90	W	1-154	[»]
3CC2	X-ray	2.40	W	1-154	[»]
3CC4	X-ray	2.70	W	1-154	[»]
3CC7	X-ray	2.70	W	1-154	[»]
3CCE	X-ray	2.75	W	1-154	[»]
3CCJ	X-ray	2.70	W	1-154	[»]
3CCL	X-ray	2.90	W	1-154	[»]
3CCM	X-ray	2.55	W	1-154	[»]
3CCQ	X-ray	2.90	W	1-154	[»]
3CCR	X-ray	3.00	W	1-154	[»]
3CCS	X-ray	2.95	W	1-154	[»]
3CCU	X-ray	2.80	W	1-154	[»]
3CCV	X-ray	2.90	W	1-154	[»]
3CD6	X-ray	2.75	W	1-154	[»]
3CMA	X-ray	2.80	W	1-154	[»]
3CME	X-ray	2.95	W	1-154	[»]
3CPW	X-ray	2.70	V	1-154	[»]
3CXC	X-ray	3.00	V	1-154	[»]
3G4S	X-ray	3.20	W	1-154	[»]
3G6E	X-ray	2.70	W	1-154	[»]
3G71	X-ray	2.85	W	1-154	[»]

ModBase

Search...

Genome annotation databases

GeneID

3127531.

GenomeReviews

Gene locus rrnAC1591 in contig AY596297_GR.

KEGG

hma:rrnAC1591.

NMPDR

fig|272569.1.peg.1482.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P14121.

OMA

DYITWGE.

Enzyme and pathway databases

BioCyc

HMAR272569:RRNAC1591-MON.

Family and domain databases

HAMAP

MF_01371.
[Tree]

InterPro

IPR005997. Ribosomal_L30_arc.
IPR018038. Ribosomal_L30_CS.
IPR000517. Ribosomal_L30p/L7e_cons-reg.
[Graphical view]

Pfam

PF00327. Ribosomal_L30. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01309. L30P_arch. 1 hit.

PROSITE

PS00634. RIBOSOMAL_L30. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL30_HALMA

Accession

Primary (citable) accession number: P14121
Secondary accession number(s): Q5V1U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	August 1, 1992
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)