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Protein

60S ribosomal protein L30

Gene

RPL30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • pre-mRNA 5'-splice site binding Source: SGD
  • RNA binding Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • negative regulation of mRNA splicing, via spliceosome Source: SGD
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30546-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L30
Alternative name(s):
L32
RP73
YL38
Gene namesi
Name:RPL30
Synonyms:RPL32
Ordered Locus Names:YGL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL030W.
SGDiS000002998. RPL30.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001461432 – 10560S ribosomal protein L30Add BLAST104

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki83Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP14120.
PRIDEiP14120.
TopDownProteomicsiP14120.

PTM databases

iPTMnetiP14120.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

Protein-protein interaction databases

BioGridi33216. 78 interactors.
DIPiDIP-1891N.
IntActiP14120. 88 interactors.
MINTiMINT-406716.

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Beta strandi21 – 25Combined sources5
Helixi26 – 35Combined sources10
Beta strandi40 – 45Combined sources6
Helixi53 – 62Combined sources10
Beta strandi67 – 70Combined sources4
Helixi79 – 87Combined sources9
Beta strandi88 – 94Combined sources7
Helixi100 – 104Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CK2NMR-A2-105[»]
1CN7NMR-A2-105[»]
1NMUX-ray2.31B/D2-105[»]
1T0KX-ray3.24B1-105[»]
3J6Xelectron microscopy6.10701-105[»]
3J6Yelectron microscopy6.10701-105[»]
3J77electron microscopy6.20801-105[»]
3J78electron microscopy6.30801-105[»]
3JCTelectron microscopy3.08c1-105[»]
4U3MX-ray3.00O0/o02-105[»]
4U3NX-ray3.20O0/o02-105[»]
4U3UX-ray2.90O0/o02-105[»]
4U4NX-ray3.10O0/o02-105[»]
4U4OX-ray3.60O0/o02-105[»]
4U4QX-ray3.00O0/o02-105[»]
4U4RX-ray2.80O0/o02-105[»]
4U4UX-ray3.00O0/o02-105[»]
4U4YX-ray3.20O0/o02-105[»]
4U4ZX-ray3.10O0/o02-105[»]
4U50X-ray3.20O0/o02-105[»]
4U51X-ray3.20O0/o02-105[»]
4U52X-ray3.00O0/o02-105[»]
4U53X-ray3.30O0/o02-105[»]
4U55X-ray3.20O0/o02-105[»]
4U56X-ray3.45O0/o02-105[»]
4U6FX-ray3.10O0/o02-105[»]
4V6Ielectron microscopy8.80Bf1-105[»]
4V7Felectron microscopy8.70b1-105[»]
4V7RX-ray4.00BZ/DZ1-105[»]
4V88X-ray3.00Bc/Dc1-105[»]
4V8Telectron microscopy8.10c1-105[»]
4V8Yelectron microscopy4.30Bc2-105[»]
4V8Zelectron microscopy6.60Bc2-105[»]
4V91electron microscopy3.70c1-105[»]
5APNelectron microscopy3.91c1-105[»]
5APOelectron microscopy3.41c1-105[»]
5DATX-ray3.15O0/o02-105[»]
5DC3X-ray3.25O0/o02-105[»]
5FCIX-ray3.40O0/o02-105[»]
5FCJX-ray3.10O0/o02-105[»]
5FL8electron microscopy9.50c1-105[»]
5GAKelectron microscopy3.88e1-105[»]
5I4LX-ray3.10O0/o06-105[»]
5JUOelectron microscopy4.00HA1-105[»]
5JUPelectron microscopy3.50HA1-105[»]
5JUSelectron microscopy4.20HA1-105[»]
5JUTelectron microscopy4.00HA1-105[»]
5JUUelectron microscopy4.00HA1-105[»]
ProteinModelPortaliP14120.
SMRiP14120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14120.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L30e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000012138.
HOGENOMiHOG000055224.
InParanoidiP14120.
KOiK02908.
OMAiAMLSKTK.
OrthoDBiEOG092C5LQM.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR000231. Ribosomal_L30e.
IPR022991. Ribosomal_L30e_CS.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR11449. PTHR11449. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS00709. RIBOSOMAL_L30E_1. 1 hit.
PS00993. RIBOSOMAL_L30E_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV
60 70 80 90 100
LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI

LTTLA
Length:105
Mass (Da):11,415
Last modified:January 23, 2007 - v3
Checksum:i963C973A02E9C8E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03457 Genomic DNA. Translation: AAA35005.1.
Z72552 Genomic DNA. Translation: CAA96731.1.
BK006941 Genomic DNA. Translation: DAA08069.1.
PIRiA29779. R6BY30.
RefSeqiNP_011485.1. NM_001180895.1.

Genome annotation databases

EnsemblFungiiYGL030W; YGL030W; YGL030W.
GeneIDi852853.
KEGGisce:YGL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03457 Genomic DNA. Translation: AAA35005.1.
Z72552 Genomic DNA. Translation: CAA96731.1.
BK006941 Genomic DNA. Translation: DAA08069.1.
PIRiA29779. R6BY30.
RefSeqiNP_011485.1. NM_001180895.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CK2NMR-A2-105[»]
1CN7NMR-A2-105[»]
1NMUX-ray2.31B/D2-105[»]
1T0KX-ray3.24B1-105[»]
3J6Xelectron microscopy6.10701-105[»]
3J6Yelectron microscopy6.10701-105[»]
3J77electron microscopy6.20801-105[»]
3J78electron microscopy6.30801-105[»]
3JCTelectron microscopy3.08c1-105[»]
4U3MX-ray3.00O0/o02-105[»]
4U3NX-ray3.20O0/o02-105[»]
4U3UX-ray2.90O0/o02-105[»]
4U4NX-ray3.10O0/o02-105[»]
4U4OX-ray3.60O0/o02-105[»]
4U4QX-ray3.00O0/o02-105[»]
4U4RX-ray2.80O0/o02-105[»]
4U4UX-ray3.00O0/o02-105[»]
4U4YX-ray3.20O0/o02-105[»]
4U4ZX-ray3.10O0/o02-105[»]
4U50X-ray3.20O0/o02-105[»]
4U51X-ray3.20O0/o02-105[»]
4U52X-ray3.00O0/o02-105[»]
4U53X-ray3.30O0/o02-105[»]
4U55X-ray3.20O0/o02-105[»]
4U56X-ray3.45O0/o02-105[»]
4U6FX-ray3.10O0/o02-105[»]
4V6Ielectron microscopy8.80Bf1-105[»]
4V7Felectron microscopy8.70b1-105[»]
4V7RX-ray4.00BZ/DZ1-105[»]
4V88X-ray3.00Bc/Dc1-105[»]
4V8Telectron microscopy8.10c1-105[»]
4V8Yelectron microscopy4.30Bc2-105[»]
4V8Zelectron microscopy6.60Bc2-105[»]
4V91electron microscopy3.70c1-105[»]
5APNelectron microscopy3.91c1-105[»]
5APOelectron microscopy3.41c1-105[»]
5DATX-ray3.15O0/o02-105[»]
5DC3X-ray3.25O0/o02-105[»]
5FCIX-ray3.40O0/o02-105[»]
5FCJX-ray3.10O0/o02-105[»]
5FL8electron microscopy9.50c1-105[»]
5GAKelectron microscopy3.88e1-105[»]
5I4LX-ray3.10O0/o06-105[»]
5JUOelectron microscopy4.00HA1-105[»]
5JUPelectron microscopy3.50HA1-105[»]
5JUSelectron microscopy4.20HA1-105[»]
5JUTelectron microscopy4.00HA1-105[»]
5JUUelectron microscopy4.00HA1-105[»]
ProteinModelPortaliP14120.
SMRiP14120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33216. 78 interactors.
DIPiDIP-1891N.
IntActiP14120. 88 interactors.
MINTiMINT-406716.

PTM databases

iPTMnetiP14120.

Proteomic databases

MaxQBiP14120.
PRIDEiP14120.
TopDownProteomicsiP14120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL030W; YGL030W; YGL030W.
GeneIDi852853.
KEGGisce:YGL030W.

Organism-specific databases

EuPathDBiFungiDB:YGL030W.
SGDiS000002998. RPL30.

Phylogenomic databases

GeneTreeiENSGT00390000012138.
HOGENOMiHOG000055224.
InParanoidiP14120.
KOiK02908.
OMAiAMLSKTK.
OrthoDBiEOG092C5LQM.

Enzyme and pathway databases

BioCyciYEAST:G3O-30546-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP14120.
PROiP14120.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR000231. Ribosomal_L30e.
IPR022991. Ribosomal_L30e_CS.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR11449. PTHR11449. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS00709. RIBOSOMAL_L30E_1. 1 hit.
PS00993. RIBOSOMAL_L30E_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRL30_YEAST
AccessioniPrimary (citable) accession number: P14120
Secondary accession number(s): D6VUA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 165 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 59300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.