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P14120

- RL30_YEAST

UniProt

P14120 - RL30_YEAST

Protein

60S ribosomal protein L30

Gene

RPL30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. pre-mRNA 5'-splice site binding Source: SGD
    2. RNA binding Source: SGD
    3. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. negative regulation of mRNA splicing, via spliceosome Source: SGD
    3. negative regulation of translation Source: SGD
    4. rRNA processing Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30546-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L30
    Alternative name(s):
    L32
    RP73
    YL38
    Gene namesi
    Name:RPL30
    Synonyms:RPL32
    Ordered Locus Names:YGL030W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    SGDiS000002998. RPL30.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 10510460S ribosomal protein L30PRO_0000146143Add
    BLAST

    Proteomic databases

    MaxQBiP14120.
    PaxDbiP14120.
    PeptideAtlasiP14120.
    PRIDEiP14120.

    Expressioni

    Gene expression databases

    GenevestigatoriP14120.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    URN1Q065251EBI-15333,EBI-35138

    Protein-protein interaction databases

    BioGridi33216. 142 interactions.
    DIPiDIP-1891N.
    IntActiP14120. 88 interactions.
    MINTiMINT-406716.
    STRINGi4932.YGL030W.

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 2014
    Beta strandi21 – 255
    Helixi26 – 3510
    Beta strandi40 – 456
    Helixi53 – 6210
    Beta strandi67 – 704
    Helixi79 – 879
    Beta strandi88 – 947
    Helixi100 – 1045

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CK2NMR-A2-105[»]
    1CN7NMR-A2-105[»]
    1NMUX-ray2.31B/D2-105[»]
    1T0KX-ray3.24B1-105[»]
    1VW8electron microscopy6.10d1-105[»]
    1VWUelectron microscopy6.10d1-105[»]
    3IZSelectron microscopy-f1-105[»]
    3J65electron microscopy8.70b1-105[»]
    3O58X-ray4.00Z1-105[»]
    3O5HX-ray4.00Z1-105[»]
    3U5EX-ray3.00c1-105[»]
    3U5IX-ray3.00c1-105[»]
    4B6Aelectron microscopy8.10c1-105[»]
    4BYNelectron microscopy4.30c2-105[»]
    4BYUelectron microscopy6.60c2-105[»]
    4CUWelectron microscopy3.70c1-105[»]
    ProteinModelPortaliP14120.
    SMRiP14120. Positions 2-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14120.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L30e family.Curated

    Phylogenomic databases

    eggNOGiCOG1911.
    GeneTreeiENSGT00390000012138.
    HOGENOMiHOG000055224.
    KOiK02908.
    OMAiMRNGKAK.
    OrthoDBiEOG76MKNV.

    Family and domain databases

    Gene3Di3.30.1330.30. 1 hit.
    InterProiIPR029064. L30e-like.
    IPR000231. Ribosomal_L30e.
    IPR022991. Ribosomal_L30e_CS.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    [Graphical view]
    PANTHERiPTHR11449. PTHR11449. 1 hit.
    PfamiPF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view]
    SUPFAMiSSF55315. SSF55315. 1 hit.
    PROSITEiPS00709. RIBOSOMAL_L30E_1. 1 hit.
    PS00993. RIBOSOMAL_L30E_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14120-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV    50
    LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI 100
    LTTLA 105
    Length:105
    Mass (Da):11,415
    Last modified:January 23, 2007 - v3
    Checksum:i963C973A02E9C8E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03457 Genomic DNA. Translation: AAA35005.1.
    Z72552 Genomic DNA. Translation: CAA96731.1.
    BK006941 Genomic DNA. Translation: DAA08069.1.
    PIRiA29779. R6BY30.
    RefSeqiNP_011485.1. NM_001180895.1.

    Genome annotation databases

    EnsemblFungiiYGL030W; YGL030W; YGL030W.
    GeneIDi852853.
    KEGGisce:YGL030W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03457 Genomic DNA. Translation: AAA35005.1 .
    Z72552 Genomic DNA. Translation: CAA96731.1 .
    BK006941 Genomic DNA. Translation: DAA08069.1 .
    PIRi A29779. R6BY30.
    RefSeqi NP_011485.1. NM_001180895.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CK2 NMR - A 2-105 [» ]
    1CN7 NMR - A 2-105 [» ]
    1NMU X-ray 2.31 B/D 2-105 [» ]
    1T0K X-ray 3.24 B 1-105 [» ]
    1VW8 electron microscopy 6.10 d 1-105 [» ]
    1VWU electron microscopy 6.10 d 1-105 [» ]
    3IZS electron microscopy - f 1-105 [» ]
    3J65 electron microscopy 8.70 b 1-105 [» ]
    3O58 X-ray 4.00 Z 1-105 [» ]
    3O5H X-ray 4.00 Z 1-105 [» ]
    3U5E X-ray 3.00 c 1-105 [» ]
    3U5I X-ray 3.00 c 1-105 [» ]
    4B6A electron microscopy 8.10 c 1-105 [» ]
    4BYN electron microscopy 4.30 c 2-105 [» ]
    4BYU electron microscopy 6.60 c 2-105 [» ]
    4CUW electron microscopy 3.70 c 1-105 [» ]
    ProteinModelPortali P14120.
    SMRi P14120. Positions 2-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33216. 142 interactions.
    DIPi DIP-1891N.
    IntActi P14120. 88 interactions.
    MINTi MINT-406716.
    STRINGi 4932.YGL030W.

    Proteomic databases

    MaxQBi P14120.
    PaxDbi P14120.
    PeptideAtlasi P14120.
    PRIDEi P14120.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL030W ; YGL030W ; YGL030W .
    GeneIDi 852853.
    KEGGi sce:YGL030W.

    Organism-specific databases

    SGDi S000002998. RPL30.

    Phylogenomic databases

    eggNOGi COG1911.
    GeneTreei ENSGT00390000012138.
    HOGENOMi HOG000055224.
    KOi K02908.
    OMAi MRNGKAK.
    OrthoDBi EOG76MKNV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30546-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P14120.
    NextBioi 972449.
    PROi P14120.

    Gene expression databases

    Genevestigatori P14120.

    Family and domain databases

    Gene3Di 3.30.1330.30. 1 hit.
    InterProi IPR029064. L30e-like.
    IPR000231. Ribosomal_L30e.
    IPR022991. Ribosomal_L30e_CS.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    [Graphical view ]
    PANTHERi PTHR11449. PTHR11449. 1 hit.
    Pfami PF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55315. SSF55315. 1 hit.
    PROSITEi PS00709. RIBOSOMAL_L30E_1. 1 hit.
    PS00993. RIBOSOMAL_L30E_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast ribosomal protein L32 and its gene."
      Dabeva M.D., Warner J.R.
      J. Biol. Chem. 262:16055-16059(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A novel loop-loop recognition motif in the yeast ribosomal protein L30 autoregulatory RNA complex."
      Mao H., White S.A., Williamson J.R.
      Nat. Struct. Biol. 6:1139-1147(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    9. "Inherent protein structural flexibility at the RNA-binding interface of L30e."
      Chao J.A., Prasad G.S., White S.A., Stout C.D., Williamson J.R.
      J. Mol. Biol. 326:999-1004(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS).
    10. "Joint X-ray and NMR refinement of the yeast L30e-mRNA complex."
      Chao J.A., Williamson J.R.
      Structure 12:1165-1176(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.24 ANGSTROMS) IN COMPLEX WITH MRNA.
    11. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL30_YEAST
    AccessioniPrimary (citable) accession number: P14120
    Secondary accession number(s): D6VUA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 141 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 59300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3