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Protein

60S ribosomal protein L30

Gene

RPL30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. pre-mRNA 5'-splice site binding Source: SGD
  2. RNA binding Source: SGD
  3. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. negative regulation of mRNA splicing, via spliceosome Source: SGD
  3. rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30546-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L30
Alternative name(s):
L32
RP73
YL38
Gene namesi
Name:RPL30
Synonyms:RPL32
Ordered Locus Names:YGL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL030W.
SGDiS000002998. RPL30.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 10510460S ribosomal protein L30PRO_0000146143Add
BLAST

Proteomic databases

MaxQBiP14120.
PaxDbiP14120.
PeptideAtlasiP14120.
PRIDEiP14120.

Expressioni

Gene expression databases

GenevestigatoriP14120.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
URN1Q065251EBI-15333,EBI-35138

Protein-protein interaction databases

BioGridi33216. 144 interactions.
DIPiDIP-1891N.
IntActiP14120. 88 interactions.
MINTiMINT-406716.
STRINGi4932.YGL030W.

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2014Combined sources
Beta strandi21 – 255Combined sources
Helixi26 – 3510Combined sources
Beta strandi40 – 456Combined sources
Helixi53 – 6210Combined sources
Beta strandi67 – 704Combined sources
Helixi79 – 879Combined sources
Beta strandi88 – 947Combined sources
Helixi100 – 1045Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CK2NMR-A2-105[»]
1CN7NMR-A2-105[»]
1NMUX-ray2.31B/D2-105[»]
1T0KX-ray3.24B1-105[»]
3J6Xelectron microscopy6.10701-105[»]
3J6Yelectron microscopy6.10701-105[»]
3J77electron microscopy6.20801-105[»]
3J78electron microscopy6.30801-105[»]
4U3MX-ray3.00O0/o02-105[»]
4U3NX-ray3.20O0/o02-105[»]
4U3UX-ray2.90O0/o02-105[»]
4U4NX-ray3.10O0/o02-105[»]
4U4OX-ray3.60O0/o02-105[»]
4U4QX-ray3.00O0/o02-105[»]
4U4RX-ray2.80O0/o02-105[»]
4U4UX-ray3.00O0/o02-105[»]
4U4YX-ray3.20O0/o02-105[»]
4U4ZX-ray3.10O0/o02-105[»]
4U50X-ray3.20O0/o02-105[»]
4U51X-ray3.20O0/o02-105[»]
4U52X-ray3.00O0/o02-105[»]
4U53X-ray3.30O0/o02-105[»]
4U55X-ray3.20O0/o02-105[»]
4U56X-ray3.45O0/o02-105[»]
4U6FX-ray3.10O0/o02-105[»]
4V6Ielectron microscopy8.80Bf1-105[»]
4V7Felectron microscopy8.70b1-105[»]
4V7RX-ray4.00BZ/DZ1-105[»]
4V88X-ray3.00Bc/Dc1-105[»]
4V8Telectron microscopy8.10c1-105[»]
4V8Yelectron microscopy4.30Bc2-105[»]
4V8Zelectron microscopy6.60Bc2-105[»]
4V91electron microscopy3.70c1-105[»]
SMRiP14120. Positions 2-105.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14120.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L30e family.Curated

Phylogenomic databases

eggNOGiCOG1911.
GeneTreeiENSGT00390000012138.
HOGENOMiHOG000055224.
InParanoidiP14120.
KOiK02908.
OMAiRCSTMAV.
OrthoDBiEOG76MKNV.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR000231. Ribosomal_L30e.
IPR022991. Ribosomal_L30e_CS.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR11449. PTHR11449. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS00709. RIBOSOMAL_L30E_1. 1 hit.
PS00993. RIBOSOMAL_L30E_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV
60 70 80 90 100
LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI

LTTLA
Length:105
Mass (Da):11,415
Last modified:January 23, 2007 - v3
Checksum:i963C973A02E9C8E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03457 Genomic DNA. Translation: AAA35005.1.
Z72552 Genomic DNA. Translation: CAA96731.1.
BK006941 Genomic DNA. Translation: DAA08069.1.
PIRiA29779. R6BY30.
RefSeqiNP_011485.1. NM_001180895.1.

Genome annotation databases

EnsemblFungiiYGL030W; YGL030W; YGL030W.
GeneIDi852853.
KEGGisce:YGL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03457 Genomic DNA. Translation: AAA35005.1.
Z72552 Genomic DNA. Translation: CAA96731.1.
BK006941 Genomic DNA. Translation: DAA08069.1.
PIRiA29779. R6BY30.
RefSeqiNP_011485.1. NM_001180895.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CK2NMR-A2-105[»]
1CN7NMR-A2-105[»]
1NMUX-ray2.31B/D2-105[»]
1T0KX-ray3.24B1-105[»]
3J6Xelectron microscopy6.10701-105[»]
3J6Yelectron microscopy6.10701-105[»]
3J77electron microscopy6.20801-105[»]
3J78electron microscopy6.30801-105[»]
4U3MX-ray3.00O0/o02-105[»]
4U3NX-ray3.20O0/o02-105[»]
4U3UX-ray2.90O0/o02-105[»]
4U4NX-ray3.10O0/o02-105[»]
4U4OX-ray3.60O0/o02-105[»]
4U4QX-ray3.00O0/o02-105[»]
4U4RX-ray2.80O0/o02-105[»]
4U4UX-ray3.00O0/o02-105[»]
4U4YX-ray3.20O0/o02-105[»]
4U4ZX-ray3.10O0/o02-105[»]
4U50X-ray3.20O0/o02-105[»]
4U51X-ray3.20O0/o02-105[»]
4U52X-ray3.00O0/o02-105[»]
4U53X-ray3.30O0/o02-105[»]
4U55X-ray3.20O0/o02-105[»]
4U56X-ray3.45O0/o02-105[»]
4U6FX-ray3.10O0/o02-105[»]
4V6Ielectron microscopy8.80Bf1-105[»]
4V7Felectron microscopy8.70b1-105[»]
4V7RX-ray4.00BZ/DZ1-105[»]
4V88X-ray3.00Bc/Dc1-105[»]
4V8Telectron microscopy8.10c1-105[»]
4V8Yelectron microscopy4.30Bc2-105[»]
4V8Zelectron microscopy6.60Bc2-105[»]
4V91electron microscopy3.70c1-105[»]
SMRiP14120. Positions 2-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33216. 144 interactions.
DIPiDIP-1891N.
IntActiP14120. 88 interactions.
MINTiMINT-406716.
STRINGi4932.YGL030W.

Proteomic databases

MaxQBiP14120.
PaxDbiP14120.
PeptideAtlasiP14120.
PRIDEiP14120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL030W; YGL030W; YGL030W.
GeneIDi852853.
KEGGisce:YGL030W.

Organism-specific databases

EuPathDBiFungiDB:YGL030W.
SGDiS000002998. RPL30.

Phylogenomic databases

eggNOGiCOG1911.
GeneTreeiENSGT00390000012138.
HOGENOMiHOG000055224.
InParanoidiP14120.
KOiK02908.
OMAiRCSTMAV.
OrthoDBiEOG76MKNV.

Enzyme and pathway databases

BioCyciYEAST:G3O-30546-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

EvolutionaryTraceiP14120.
NextBioi972449.
PROiP14120.

Gene expression databases

GenevestigatoriP14120.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR000231. Ribosomal_L30e.
IPR022991. Ribosomal_L30e_CS.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR11449. PTHR11449. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS00709. RIBOSOMAL_L30E_1. 1 hit.
PS00993. RIBOSOMAL_L30E_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast ribosomal protein L32 and its gene."
    Dabeva M.D., Warner J.R.
    J. Biol. Chem. 262:16055-16059(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A novel loop-loop recognition motif in the yeast ribosomal protein L30 autoregulatory RNA complex."
    Mao H., White S.A., Williamson J.R.
    Nat. Struct. Biol. 6:1139-1147(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  9. "Inherent protein structural flexibility at the RNA-binding interface of L30e."
    Chao J.A., Prasad G.S., White S.A., Stout C.D., Williamson J.R.
    J. Mol. Biol. 326:999-1004(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS).
  10. "Joint X-ray and NMR refinement of the yeast L30e-mRNA complex."
    Chao J.A., Williamson J.R.
    Structure 12:1165-1176(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.24 ANGSTROMS) IN COMPLEX WITH MRNA.
  11. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL30_YEAST
AccessioniPrimary (citable) accession number: P14120
Secondary accession number(s): D6VUA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 59300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.