60S ribosomal protein L30 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P14120 (RL30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L30

Alternative name(s):
L32
RP73
YL38

Gene names

Name:	RPL30
Synonyms:	RPL32
Ordered Locus Names:	YGL030W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Present with 59300 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the ribosomal protein L30e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Traceable author statement Ref.4. Source: SGD negative regulation of nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype. Source: SGD negative regulation of translation Traceable author statement. Source: SGD rRNA processing Inferred from mutant phenotype. Source: SGD
Cellular component	cytosolic large ribosomal subunit Traceable author statement Ref.4. Source: SGD
Molecular function	pre-mRNA 5'-splice site binding Inferred from mutant phenotype. Source: SGD structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
URN1	Q06525	1	EBI-15333,EBI-35138

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1 Ref.5

Chain

2 – 105

104

60S ribosomal protein L30

PRO_0000146143

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Secondary structure

105

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P14120 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 963C973A02E9C8E5
Show »

FASTA

105

11,415

        10         20         30         40         50         60 
MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA 

        70         80         90        100 
MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI LTTLA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast ribosomal protein L32 and its gene." Dabeva M.D., Warner J.R. J. Biol. Chem. 262:16055-16059(1987) [PubMed: 3316213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, MASS SPECTROMETRY.
[8]	"A novel loop-loop recognition motif in the yeast ribosomal protein L30 autoregulatory RNA complex." Mao H., White S.A., Williamson J.R. Nat. Struct. Biol. 6:1139-1147(1999) [PubMed: 10581556] [Abstract] Cited for: STRUCTURE BY NMR.
[9]	"Inherent protein structural flexibility at the RNA-binding interface of L30e." Chao J.A., Prasad G.S., White S.A., Stout C.D., Williamson J.R. J. Mol. Biol. 326:999-1004(2003) [PubMed: 12589748] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS).
[10]	"Joint X-ray and NMR refinement of the yeast L30e-mRNA complex." Chao J.A., Williamson J.R. Structure 12:1165-1176(2004) [PubMed: 15242593] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.24 ANGSTROMS) IN COMPLEX WITH MRNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J03457 Genomic DNA. Translation: AAA35005.1.
Z72552 Genomic DNA. Translation: CAA96731.1.
BK006941 Genomic DNA. Translation: DAA08069.1.

PIR

R6BY30. A29779.

RefSeq

NP_011485.1. NM_001180895.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CK2	NMR	-	A	2-104	[»]
1CN7	NMR	-	A	2-105	[»]
1NMU	X-ray	2.31	B/D	2-105	[»]
1T0K	X-ray	3.24	B	1-105	[»]
3IZS	electron microscopy	-	f	1-105	[»]
3O58	X-ray	4.00	Z	1-105	[»]
3O5H	X-ray	4.00	Z	1-105	[»]

ProteinModelPortal

P14120.

SMR

P14120. Positions 2-105.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1891N.

IntAct

P14120. 85 interactions.

MINT

MINT-406716.

STRING

P14120.

Proteomic databases

PeptideAtlas

P14120.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL030W; YGL030W; YGL030W.

GeneID

852853.

KEGG

sce:YGL030W.

NMPDR

fig|4932.3.peg.2592.

Organism-specific databases

SGD

S000002998. RPL30.

Phylogenomic databases

eggNOG

fuNOG11834.

GeneTree

EFGT00050000006702.

HOGENOM

HBG553631.

OMA

ILISTNC.

OrthoDB

EOG49GPS7.

Gene expression databases

ArrayExpress

P14120.

Genevestigator

P14120.

GermOnline

YGL030W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000231. Ribosomal_L30e.
IPR022991. Ribosomal_L30e_CS.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]

K02908.

PANTHER

PTHR11449. Ribosomal_L30e. 1 hit.

Pfam

PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]

PROSITE

PS00709. RIBOSOMAL_L30E_1. 1 hit.
PS00993. RIBOSOMAL_L30E_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

972449.

Entry information

Entry name

RL30_YEAST

Accession

Primary (citable) accession number: P14120
Secondary accession number(s): D6VUA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 120 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents