P14120 (RL30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L30

Alternative name(s):
L32
RP73
YL38

Gene names

Name:	RPL30
Synonyms:	RPL32
Ordered Locus Names:	YGL030W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Present with 59300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ribosomal protein L30e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.4. Source: SGD negative regulation of mRNA splicing, via spliceosome Inferred from mutant phenotype PubMed 18570876PubMed 9121443. Source: SGD negative regulation of translation Traceable author statement PubMed 8643676PubMed 9121443. Source: SGD rRNA processing Inferred from mutant phenotype PubMed 9121443. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.4. Source: SGD
Molecular_function	pre-mRNA 5'-splice site binding Inferred from mutant phenotype PubMed 18570876. Source: SGD structural constituent of ribosome Inferred from direct assay PubMed 9121443. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
URN1	Q06525	1	EBI-15333,EBI-35138

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.5

Chain

2 – 105

104

60S ribosomal protein L30

PRO_0000146143

Amino acid modifications

Modified residue

6

1

Phosphoserine Ref.7

Secondary structure

1

.

.

.

.

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.

.

.

.

.

.

.

.

.

.

.

105

Helix Strand Turn

Details...

Helix

7 – 20

14

Beta strand

21 – 25

5

Helix

26 – 35

10

Beta strand

40 – 45

6

Helix

53 – 62

10

Beta strand

67 – 70

4

Helix

79 – 87

9

Beta strand

88 – 94

7

Helix

100 – 104

5

Sequences

	Sequence		Length	Mass (Da)
	P14120 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 963C973A02E9C8E5	FASTA	105	11,415
	10 20 30 40 50 60 MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA 70 80 90 100 MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI LTTLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast ribosomal protein L32 and its gene." Dabeva M.D., Warner J.R. J. Biol. Chem. 262:16055-16059(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, MASS SPECTROMETRY.
[8]	"A novel loop-loop recognition motif in the yeast ribosomal protein L30 autoregulatory RNA complex." Mao H., White S.A., Williamson J.R. Nat. Struct. Biol. 6:1139-1147(1999) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[9]	"Inherent protein structural flexibility at the RNA-binding interface of L30e." Chao J.A., Prasad G.S., White S.A., Stout C.D., Williamson J.R. J. Mol. Biol. 326:999-1004(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS).
[10]	"Joint X-ray and NMR refinement of the yeast L30e-mRNA complex." Chao J.A., Williamson J.R. Structure 12:1165-1176(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.24 ANGSTROMS) IN COMPLEX WITH MRNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J03457 Genomic DNA. Translation: AAA35005.1.
Z72552 Genomic DNA. Translation: CAA96731.1.
BK006941 Genomic DNA. Translation: DAA08069.1.

PIR

R6BY30. A29779.

RefSeq

NP_011485.1. NM_001180895.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CK2	NMR	-	A	2-104	[»]
1CN7	NMR	-	A	2-105	[»]
1NMU	X-ray	2.31	B/D	2-105	[»]
1T0K	X-ray	3.24	B	1-105	[»]
3IZS	electron microscopy	-	f	1-105	[»]
3O58	X-ray	4.00	Z	1-105	[»]
3O5H	X-ray	4.00	Z	1-105	[»]
3U5E	X-ray	3.00	c	1-105	[»]
3U5I	X-ray	3.00	c	1-105	[»]
4B6A	electron microscopy	8.10	c	1-105	[»]

ProteinModelPortal

P14120.

SMR

P14120. Positions 2-105.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1891N.

IntAct

P14120. 85 interactions.

MINT

MINT-406716.

STRING

4932.YGL030W.

Proteomic databases

PaxDb

P14120.

PeptideAtlas

P14120.

PRIDE

P14120.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL030W; YGL030W; YGL030W.

GeneID

852853.

KEGG

sce:YGL030W.

Organism-specific databases

SGD

S000002998. RPL30.

Phylogenomic databases

eggNOG

COG1911.

GeneTree

ENSGT00390000012138.

HOGENOM

HOG000055224.

KO

K02908.

OMA

KYFRVCT.

OrthoDB

EOG49GPS7.

Gene expression databases

Genevestigator

P14120.

GermOnline

YGL030W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000231. Ribosomal_L30e.
IPR022991. Ribosomal_L30e_CS.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]

PANTHER

PTHR11449. PTHR11449. 1 hit.

Pfam

PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]

PROSITE

PS00709. RIBOSOMAL_L30E_1. 1 hit.
PS00993. RIBOSOMAL_L30E_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14120.

NextBio

972449.

Entry information

Entry name

RL30_YEAST

Accession

Primary (citable) accession number: P14120
Secondary accession number(s): D6VUA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program