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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Gene

PDE1A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei219Proton donorBy similarity1
Metal bindingi223Divalent metal cation 1By similarity1
Metal bindingi259Divalent metal cation 1By similarity1
Metal bindingi260Divalent metal cation 1By similarity1
Metal bindingi260Divalent metal cation 2By similarity1
Metal bindingi366Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calmodulin-binding, cAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-111957. Cam-PDE 1 activation.
R-BTA-418457. cGMP effects.
R-BTA-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A (EC:3.1.4.17)
Short name:
Cam-PDE 1A
Alternative name(s):
61 kDa Cam-PDE
Gene namesi
Name:PDE1A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 2

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3774.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987841 – 530Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1AAdd BLAST530

Proteomic databases

PaxDbiP14100.
PRIDEiP14100.

PTM databases

iPTMnetiP14100.

Expressioni

Gene expression databases

BgeeiENSBTAG00000012100.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HpcaP840762EBI-907809,EBI-908193From a different organism.
NCALDP616022EBI-907809,EBI-908133
Ncs1P621682EBI-907809,EBI-907774From a different organism.

Protein-protein interaction databases

BioGridi159260. 2 interactors.
IntActiP14100. 3 interactors.
MINTiMINT-1339090.
STRINGi9913.ENSBTAP00000051204.

Chemistry databases

BindingDBiP14100.

Structurei

3D structure databases

ProteinModelPortaliP14100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 44Calmodulin-bindingAdd BLAST21
Regioni193 – 501CatalyticBy similarityAdd BLAST309

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3688. Eukaryota.
ENOG410XQDD. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiP14100.
KOiK13755.
OMAiNRLRCLV.
OrthoDBiEOG091G04C4.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P14100-1) [UniParc]FASTAAdd to basket
Also known as: PDE1A2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSTATETEE LENTTFKYLI GEQTEKMWQR LKGILRCLVK QLEKGDVNVI
60 70 80 90 100
DLKKNIEYAA SVLEAVYIDE TRRLLDTDDE LSDIQSDSVP SEVRDWLAST
110 120 130 140 150
FTRKMGMMKK KSEEKPRFRS IVHVVQAGIF VERMYRKSYH MVGLAYPEAV
160 170 180 190 200
IVTLKDVDKW SFDVFALNEA SGEHSLKFMI YELFTRYDLI NRFKIPVSCL
210 220 230 240 250
IAFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI MHWLTELEIL
260 270 280 290 300
AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM
310 320 330 340 350
QEEEMNVLIN LSKDDWRDLR NLVIEMVLST DMSGHFQQIK NIRNSLQQPE
360 370 380 390 400
GLDKAKTMSL ILHAADISHP AKSWKLHHRW TMALMEEFFL QGDKEAELGL
410 420 430 440 450
PFSPLCDRKS TMVAQSQIGF IDFIVEPTFS LLTDSTEKII IPLIEEDSKT
460 470 480 490 500
KTPSYGASRR SNMKGTTNDG TYSPDYSLAS VDLKSFKNSL VDIIQQNKER
510 520 530
WKELAAQGEP DPHKNSDLVN AEEKHAETHS
Length:530
Mass (Da):60,843
Last modified:January 23, 2007 - v3
Checksum:i24CF83E5211AE06F
GO
Isoform 1 (identifier: P14100-2) [UniParc]FASTAAdd to basket
Also known as: PDE1A1

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MGSTATETEELENTTFKYLIGEQTEKMWQRLKGI → MDDHVTIRRKHLQRPIFR

Show »
Length:514
Mass (Da):59,198
Checksum:i7CE7D8A1C366CCE5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti237H → G AA sequence (PubMed:3025833).Curated1
Sequence conflicti321N → W AA sequence (PubMed:3025833).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0045461 – 34MGSTA…RLKGI → MDDHVTIRRKHLQRPIFR in isoform 1. 2 PublicationsAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90358 mRNA. Translation: AAA74560.1.
L34069 mRNA. Translation: AAA92555.1.
BC123449 mRNA. Translation: AAI23450.1.
PIRiA45334.
RefSeqiNP_776839.1. NM_174414.3. [P14100-2]
XP_010800147.1. XM_010801845.1. [P14100-1]
UniGeneiBt.512.

Genome annotation databases

EnsembliENSBTAT00000016060; ENSBTAP00000016060; ENSBTAG00000012100. [P14100-2]
ENSBTAT00000052318; ENSBTAP00000051204; ENSBTAG00000012100. [P14100-1]
GeneIDi281969.
KEGGibta:281969.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90358 mRNA. Translation: AAA74560.1.
L34069 mRNA. Translation: AAA92555.1.
BC123449 mRNA. Translation: AAI23450.1.
PIRiA45334.
RefSeqiNP_776839.1. NM_174414.3. [P14100-2]
XP_010800147.1. XM_010801845.1. [P14100-1]
UniGeneiBt.512.

3D structure databases

ProteinModelPortaliP14100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159260. 2 interactors.
IntActiP14100. 3 interactors.
MINTiMINT-1339090.
STRINGi9913.ENSBTAP00000051204.

Chemistry databases

BindingDBiP14100.
ChEMBLiCHEMBL3774.

PTM databases

iPTMnetiP14100.

Proteomic databases

PaxDbiP14100.
PRIDEiP14100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000016060; ENSBTAP00000016060; ENSBTAG00000012100. [P14100-2]
ENSBTAT00000052318; ENSBTAP00000051204; ENSBTAG00000012100. [P14100-1]
GeneIDi281969.
KEGGibta:281969.

Organism-specific databases

CTDi5136.

Phylogenomic databases

eggNOGiKOG3688. Eukaryota.
ENOG410XQDD. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiP14100.
KOiK13755.
OMAiNRLRCLV.
OrthoDBiEOG091G04C4.
TreeFamiTF314638.

Enzyme and pathway databases

ReactomeiR-BTA-111957. Cam-PDE 1 activation.
R-BTA-418457. cGMP effects.
R-BTA-418555. G alpha (s) signalling events.

Gene expression databases

BgeeiENSBTAG00000012100.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDE1A_BOVIN
AccessioniPrimary (citable) accession number: P14100
Secondary accession number(s): Q08E30, Q28063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.