Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P14100 (PDE1A_BOVIN)

Last modified September 2, 2008. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
      Short name=Cam-PDE 1A
    EC=3.1.4.17
Alternative name(s):
    61 kDa Cam-PDE
Gene names
Name: PDE1A
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate.

Enzyme regulation

Type I PDE are activated by the binding of calmodulin in the presence of Ca(2+).

Subunit structure

Homodimer.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords

   Coding sequence diversityAlternative splicing
   LigandCalmodulin-binding
cAMP
cGMP
   Molecular functionHydrolase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FreqP621681EBI-907809,EBI-907774From a different organism.
HpcaP840761EBI-907809,EBI-908193From a different organism.
NCALDP616021EBI-907809,EBI-908133

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: P14100-1)

Also known as: PDE1A2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P14100-2)

Also known as: PDE1A1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MGSTATETEELENTTFKYLIGEQTEKMWQRLKGI → MDDHVTIRRKHLQRPIFR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Initiator methionine11Removed
Chain2 – 530529Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Regions

Region24 – 4421Calmodulin-binding
Region193 – 501309Catalytic By similarity

Natural variations

Alternative sequence1 – 3434MGSTA…RLKGI → MDDHVTIRRKHLQRPIFR in isoform 1.

Experimental info

Sequence conflict2371H → G AA sequence Ref.5
Sequence conflict3211N → W AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (PDE1A2) [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 24CF83E5211AE06F

FASTA53060,843
        10         20         30         40         50         60 
MGSTATETEE LENTTFKYLI GEQTEKMWQR LKGILRCLVK QLEKGDVNVI DLKKNIEYAA 

        70         80         90        100        110        120 
SVLEAVYIDE TRRLLDTDDE LSDIQSDSVP SEVRDWLAST FTRKMGMMKK KSEEKPRFRS 

       130        140        150        160        170        180 
IVHVVQAGIF VERMYRKSYH MVGLAYPEAV IVTLKDVDKW SFDVFALNEA SGEHSLKFMI 

       190        200        210        220        230        240 
YELFTRYDLI NRFKIPVSCL IAFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI 

       250        260        270        280        290        300 
MHWLTELEIL AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM 

       310        320        330        340        350        360 
QEEEMNVLIN LSKDDWRDLR NLVIEMVLST DMSGHFQQIK NIRNSLQQPE GLDKAKTMSL 

       370        380        390        400        410        420 
ILHAADISHP AKSWKLHHRW TMALMEEFFL QGDKEAELGL PFSPLCDRKS TMVAQSQIGF 

       430        440        450        460        470        480 
IDFIVEPTFS LLTDSTEKII IPLIEEDSKT KTPSYGASRR SNMKGTTNDG TYSPDYSLAS 

       490        500        510        520        530 
VDLKSFKNSL VDIIQQNKER WKELAAQGEP DPHKNSDLVN AEEKHAETHS 

« Hide

Isoform 1 (PDE1A1) [UniParc].

Checksum: 7CE7D8A1C366CCE5
Show »

51459,198

References

« Hide 'large scale' references
[1]"Molecular cloning of a cDNA encoding the '61-kDa' calmodulin-stimulated cyclic nucleotide phosphodiesterase. Tissue-specific expression of structurally related isoforms."
Sonnenburg W.K., Seger D., Beavo J.A.
J. Biol. Chem. 268:645-652(1993) [PubMed: 7678006] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Identification of inhibitory and calmodulin-binding domains of the PDE1A1 and PDE1A2 calmodulin-stimulated cyclic nucleotide phosphodiesterases."
Sonnenburg W.K., Seger D., Kwak K.S., Huang J., Charbonneau H., Beavo J.A.
J. Biol. Chem. 270:30989-31000(1995) [PubMed: 8537356] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Lung.
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Hereford.
Tissue: Hippocampus.
[4]"Evidence for domain organization within the 61-kDa calmodulin-dependent cyclic nucleotide phosphodiesterase from bovine brain."
Charbonneau H., Kumar S., Novack J.P., Blumenthal D.K., Griffin P.R., Shabanowitz J., Hunt D.F., Beavo J.A., Walsh K.A.
Biochemistry 30:7931-7940(1991) [PubMed: 1651111] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-530 (ISOFORM 2).
Tissue: Brain.
[5]"Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species."
Charbonneau H., Beier N., Walsh K.A., Beavo J.A.
Proc. Natl. Acad. Sci. U.S.A. 83:9308-9312(1986) [PubMed: 3025833] [Abstract]
Cited for: PROTEIN SEQUENCE OF 194-427.
Tissue: Brain.

Cross-references

Sequence databases

M90358 mRNA. Translation: AAA74560.1.
L34069 mRNA. Translation: AAA92555.1.
BC123449 mRNA. Translation: AAI23450.1.
PIRA45334.
RefSeqNP_776839.1.
UniGeneBt.512

3D structure databases

HSSPHSSP built from PDB template 1OYN based on UniProtKB Q08499.
SMRP14100. Positions 142-471.
ModBaseSearch...

Protein-protein interaction databases

IntActP14100.

Genome annotation databases

EnsemblENSBTAG00000012100. Bos taurus. [Contig view]
GeneID281969.
KEGGbta:281969.

Phylogenomic databases

HOVERGENP14100.

Family and domain databases

InterProIPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
IPR013706. PDEase_N.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProDomP14100.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

Entry information

Entry namePDE1A_BOVIN
AccessionPrimary (citable) accession number: P14100
Secondary accession number(s): Q08E30, Q28063
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: September 2, 2008
This is version 70 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents