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P14100

- PDE1A_BOVIN

UniProt

P14100 - PDE1A_BOVIN

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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Gene

PDE1A

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei219 – 2191Proton donorBy similarity
Metal bindingi223 – 2231Divalent metal cation 1By similarity
Metal bindingi259 – 2591Divalent metal cation 1By similarity
Metal bindingi260 – 2601Divalent metal cation 1By similarity
Metal bindingi260 – 2601Divalent metal cation 2By similarity
Metal bindingi366 – 3661Divalent metal cation 1By similarity

GO - Molecular functioni

  1. calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
  2. calmodulin-dependent cyclic-nucleotide phosphodiesterase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. metabolic process Source: GOC
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calmodulin-binding, cAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_205403. cGMP effects.
REACT_206547. G alpha (s) signalling events.
REACT_212942. Cam-PDE 1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A (EC:3.1.4.17)
Short name:
Cam-PDE 1A
Alternative name(s):
61 kDa Cam-PDE
Gene namesi
Name:PDE1A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 530529Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1APRO_0000198784Add
BLAST

Proteomic databases

PRIDEiP14100.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HpcaP840762EBI-907809,EBI-908193From a different organism.
NCALDP616022EBI-907809,EBI-908133
Ncs1P621682EBI-907809,EBI-907774From a different organism.

Protein-protein interaction databases

BioGridi159260. 2 interactions.
IntActiP14100. 3 interactions.
MINTiMINT-1339090.

Structurei

3D structure databases

ProteinModelPortaliP14100.
SMRiP14100. Positions 142-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 4421Calmodulin-bindingAdd
BLAST
Regioni193 – 501309CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG139098.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000231888.
HOVERGENiHBG056120.
InParanoidiP14100.
KOiK13755.
OMAiQEEEMNV.
OrthoDBiEOG7X9G6J.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: P14100-1) [UniParc]FASTAAdd to Basket

Also known as: PDE1A2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSTATETEE LENTTFKYLI GEQTEKMWQR LKGILRCLVK QLEKGDVNVI
60 70 80 90 100
DLKKNIEYAA SVLEAVYIDE TRRLLDTDDE LSDIQSDSVP SEVRDWLAST
110 120 130 140 150
FTRKMGMMKK KSEEKPRFRS IVHVVQAGIF VERMYRKSYH MVGLAYPEAV
160 170 180 190 200
IVTLKDVDKW SFDVFALNEA SGEHSLKFMI YELFTRYDLI NRFKIPVSCL
210 220 230 240 250
IAFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI MHWLTELEIL
260 270 280 290 300
AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM
310 320 330 340 350
QEEEMNVLIN LSKDDWRDLR NLVIEMVLST DMSGHFQQIK NIRNSLQQPE
360 370 380 390 400
GLDKAKTMSL ILHAADISHP AKSWKLHHRW TMALMEEFFL QGDKEAELGL
410 420 430 440 450
PFSPLCDRKS TMVAQSQIGF IDFIVEPTFS LLTDSTEKII IPLIEEDSKT
460 470 480 490 500
KTPSYGASRR SNMKGTTNDG TYSPDYSLAS VDLKSFKNSL VDIIQQNKER
510 520 530
WKELAAQGEP DPHKNSDLVN AEEKHAETHS
Length:530
Mass (Da):60,843
Last modified:January 23, 2007 - v3
Checksum:i24CF83E5211AE06F
GO
Isoform 1 (identifier: P14100-2) [UniParc]FASTAAdd to Basket

Also known as: PDE1A1

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MGSTATETEELENTTFKYLIGEQTEKMWQRLKGI → MDDHVTIRRKHLQRPIFR

Show »
Length:514
Mass (Da):59,198
Checksum:i7CE7D8A1C366CCE5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti237 – 2371H → G AA sequence (PubMed:3025833)Curated
Sequence conflicti321 – 3211N → W AA sequence (PubMed:3025833)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3434MGSTA…RLKGI → MDDHVTIRRKHLQRPIFR in isoform 1. 2 PublicationsVSP_004546Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90358 mRNA. Translation: AAA74560.1.
L34069 mRNA. Translation: AAA92555.1.
BC123449 mRNA. Translation: AAI23450.1.
PIRiA45334.
RefSeqiNP_776839.1. NM_174414.3. [P14100-2]
XP_005202315.1. XM_005202258.1. [P14100-1]
UniGeneiBt.512.

Genome annotation databases

EnsembliENSBTAT00000016060; ENSBTAP00000016060; ENSBTAG00000012100. [P14100-2]
ENSBTAT00000052318; ENSBTAP00000051204; ENSBTAG00000012100. [P14100-1]
GeneIDi281969.
KEGGibta:281969.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90358 mRNA. Translation: AAA74560.1 .
L34069 mRNA. Translation: AAA92555.1 .
BC123449 mRNA. Translation: AAI23450.1 .
PIRi A45334.
RefSeqi NP_776839.1. NM_174414.3. [P14100-2 ]
XP_005202315.1. XM_005202258.1. [P14100-1 ]
UniGenei Bt.512.

3D structure databases

ProteinModelPortali P14100.
SMRi P14100. Positions 142-506.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 159260. 2 interactions.
IntActi P14100. 3 interactions.
MINTi MINT-1339090.

Chemistry

BindingDBi P14100.
ChEMBLi CHEMBL2111408.

Proteomic databases

PRIDEi P14100.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000016060 ; ENSBTAP00000016060 ; ENSBTAG00000012100 . [P14100-2 ]
ENSBTAT00000052318 ; ENSBTAP00000051204 ; ENSBTAG00000012100 . [P14100-1 ]
GeneIDi 281969.
KEGGi bta:281969.

Organism-specific databases

CTDi 5136.

Phylogenomic databases

eggNOGi NOG139098.
GeneTreei ENSGT00760000118889.
HOGENOMi HOG000231888.
HOVERGENi HBG056120.
InParanoidi P14100.
KOi K13755.
OMAi QEEEMNV.
OrthoDBi EOG7X9G6J.
TreeFami TF314638.

Enzyme and pathway databases

Reactomei REACT_205403. cGMP effects.
REACT_206547. G alpha (s) signalling events.
REACT_212942. Cam-PDE 1 activation.

Miscellaneous databases

NextBioi 20805840.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR013706. PDEase_N.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
PF08499. PDEase_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a cDNA encoding the '61-kDa' calmodulin-stimulated cyclic nucleotide phosphodiesterase. Tissue-specific expression of structurally related isoforms."
    Sonnenburg W.K., Seger D., Beavo J.A.
    J. Biol. Chem. 268:645-652(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Identification of inhibitory and calmodulin-binding domains of the PDE1A1 and PDE1A2 calmodulin-stimulated cyclic nucleotide phosphodiesterases."
    Sonnenburg W.K., Seger D., Kwak K.S., Huang J., Charbonneau H., Beavo J.A.
    J. Biol. Chem. 270:30989-31000(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Lung.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Hereford.
    Tissue: Hippocampus.
  4. "Evidence for domain organization within the 61-kDa calmodulin-dependent cyclic nucleotide phosphodiesterase from bovine brain."
    Charbonneau H., Kumar S., Novack J.P., Blumenthal D.K., Griffin P.R., Shabanowitz J., Hunt D.F., Beavo J.A., Walsh K.A.
    Biochemistry 30:7931-7940(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-530 (ISOFORM 2).
    Tissue: Brain.
  5. "Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species."
    Charbonneau H., Beier N., Walsh K.A., Beavo J.A.
    Proc. Natl. Acad. Sci. U.S.A. 83:9308-9312(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 194-427.
    Tissue: Brain.

Entry informationi

Entry nameiPDE1A_BOVIN
AccessioniPrimary (citable) accession number: P14100
Secondary accession number(s): Q08E30, Q28063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3