P14095 (GROA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Growth-regulated alpha protein

Alternative name(s):
C-X-C motif chemokine 1
Cytokine-induced neutrophil chemoattractant 1
Short name=CINC-1
Platelet-derived growth factor-inducible protein KC

Gene names

Name:	Cxcl1
Synonyms:	Cinc1, Gro, Scyb1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Subunit structure	Monomer and homodimer.
Subcellular location	Secreted.
Tissue specificity	At least expressed in the lung and trachea.
Induction	By lipopolysaccharides (LPS) and inflammation.
Sequence similarities	Belongs to the intercrine alpha (chemokine CxC) family.

Ontologies

Keywords
Biological process	Inflammatory response
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acute inflammatory response Inferred from direct assay PubMed 19497959. Source: RGD elevation of cytosolic calcium ion concentration Inferred from direct assay PubMed 10975996. Source: RGD immune response Inferred from electronic annotation. Source: InterPro neutrophil chemotaxis Inferred from direct assay PubMed 12709409. Source: RGD positive regulation of potassium ion transport Inferred from direct assay PubMed 19476648. Source: RGD positive regulation of sodium ion transport Inferred from direct assay PubMed 18816377. Source: RGD response to amphetamine Inferred from expression pattern PubMed 19582783. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 20709317. Source: RGD response to gamma radiation Inferred from expression pattern PubMed 20185578. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 20728373. Source: RGD response to heat Inferred from expression pattern PubMed 19582783. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 19390478. Source: RGD
Cellular_component	extracellular space Inferred from direct assay PubMed 21442011. Source: RGD
Molecular_function	chemokine activity Inferred from direct assay PubMed 10975996. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.3 Ref.4
	Chain	25 – 96	72	Growth-regulated alpha protein		PRO_0000005055
Amino acid modifications
	Disulfide bond	33 ↔ 59		By similarity
	Disulfide bond	35 ↔ 75		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14095 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 6046C9E2C2582652	FASTA	96	10,249
	10 20 30 40 50 60 MVSATRSLLC AALPVLATSR QATGAPVANE LRCQCLQTVA GIHFKNIQSL KVMPPGPHCT 70 80 90 QTEVIATLKN GREACLDPEA PMVQKIVQKM LKGVPK

References

[1]	"Structure of the gene encoding rat neutrophil chemo-attractant Gro." Konishi K., Tanaka Y., Yamamoto M., Yomogida K., Watanabe K., Tsurufuji S., Fujioka M. Gene 126:285-286(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts." Huang S., Paulauskis J.D., Kobzik L. Biochem. Biophys. Res. Commun. 184:922-929(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The neutrophil chemoattractant produced by the rat kidney epithelioid cell line NRK-52E is a protein related to the KC/gro protein." Watanabe K., Konishi K., Fujioka M., Kinoshita S., Nakagawa H. J. Biol. Chem. 264:19559-19563(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-96.
[4]	"Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture." Nakagawa H., Shiota S., Takano K., Shibata F., Kato H. Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-56. Strain: Wistar.
[5]	"Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary inflammation." Huang S., Paulauskis J.D., Godleski J.J., Kobzik L. Am. J. Pathol. 141:981-988(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-88. Tissue: Lung.
[6]	"The three dimensional structure of rat cytokine CINC/Gro in solution by homonuclear 3D NMR." Hanzawa H., Haruyama H., Watanabe K., Tsurufuji S. FEBS Lett. 354:207-212(1994) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[7]	"Subunit association and monomer structure of CINC/Gro revealed by 1H-NMR." Hanzawa H., Haruyama H., Konishi K., Watanabe K., Tsurufuji S. J. Biochem. 121:835-841(1997) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[8]	"Solution structure of CINC/Gro investigated by heteronuclear NMR." Hanzawa H., Haruyama H., Konishi K., Watanabe K., Tsurufuji S. J. Biochem. 123:62-70(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11445 Genomic DNA. Translation: BAA02009.1. D11444 mRNA. Translation: BAA02008.1. M86536 mRNA. Translation: AAA42053.1. S45856 mRNA. No translation available.
IPI	IPI00201415.
PIR	JN0572.
RefSeq	NP_110472.1. NM_030845.1.
UniGene	Rn.10907.
3D structure databases
ProteinModelPortal	P14095.
SMR	P14095. Positions 30-92.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000003778.
Proteomic databases
PaxDb	P14095.
PRIDE	P14095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81503.
KEGG	rno:81503.
UCSC	RGD:619869. rat.
Organism-specific databases
CTD	2919.
RGD	619869. Cxcl1.
Phylogenomic databases
eggNOG	NOG83801.
HOGENOM	HOG000220915.
HOVERGEN	HBG107789.
InParanoid	P14095.
KO	K05505.
OrthoDB	EOG4Z8XZ6.
Gene expression databases
Genevestigator	P14095.
GermOnline	ENSRNOG00000002802. Rattus norvegicus.
Family and domain databases
InterPro	IPR001089. Chemokine_CXC. IPR018048. Chemokine_CXC_CS. IPR001811. Chemokine_IL8-like_dom. [Graphical view]
PANTHER	PTHR10179. PTHR10179. 1 hit.
Pfam	PF00048. IL8. 1 hit. [Graphical view]
PRINTS	PR00437. SMALLCYTKCXC.
SMART	SM00199. SCY. 1 hit. [Graphical view]
SUPFAM	SSF54117. Chemokine_IL8. 1 hit.
PROSITE	PS00471. SMALL_CYTOKINES_CXC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	614955.

Entry information

Entry name

GROA_RAT

Accession

Primary (citable) accession number: P14095

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program