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Protein

Sodium/potassium-transporting ATPase subunit beta-1

Gene

Atp1b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Involved in cell adhesion and establishing epithelial cell polarity.By similarity

GO - Molecular functioni

GO - Biological processi

  • ATP hydrolysis coupled transmembrane transport Source: MGI
  • ATP metabolic process Source: MGI
  • cardiac muscle contraction Source: BHF-UCL
  • cell adhesion Source: UniProtKB-KW
  • cellular calcium ion homeostasis Source: BHF-UCL
  • cellular potassium ion homeostasis Source: MGI
  • cellular sodium ion homeostasis Source: MGI
  • establishment or maintenance of transmembrane electrochemical gradient Source: MGI
  • membrane repolarization Source: MGI
  • mitophagy in response to mitochondrial depolarization Source: MGI
  • positive regulation of ATPase activity Source: MGI
  • positive regulation of calcium:sodium antiporter activity Source: BHF-UCL
  • positive regulation of defense response to virus by host Source: MGI
  • positive regulation of potassium ion import Source: MGI
  • positive regulation of potassium ion transmembrane transporter activity Source: MGI
  • positive regulation of sodium ion export from cell Source: MGI
  • potassium ion import Source: BHF-UCL
  • protein localization to plasma membrane Source: MGI
  • protein stabilization Source: BHF-UCL
  • regulation of calcium ion transmembrane transport Source: BHF-UCL
  • regulation of cardiac muscle contraction by calcium ion signaling Source: BHF-UCL
  • regulation of gene expression Source: BHF-UCL
  • relaxation of cardiac muscle Source: BHF-UCL
  • response to hypoxia Source: Ensembl
  • sodium ion export from cell Source: BHF-UCL
  • xenophagy Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-210991. Basigin interactions.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1
Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1
Gene namesi
Name:Atp1b1
Synonyms:Atp4b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88108. Atp1b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434CytoplasmicSequence analysisAdd
BLAST
Transmembranei35 – 6228Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini63 – 304242ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • basolateral plasma membrane Source: MGI
  • caveola Source: Ensembl
  • extracellular exosome Source: MGI
  • extracellular vesicle Source: MGI
  • intercalated disc Source: BHF-UCL
  • intracellular Source: GOC
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • plasma membrane Source: MGI
  • sarcolemma Source: BHF-UCL
  • sodium:potassium-exchanging ATPase complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Sodium/potassium-transporting ATPase subunit beta-1PRO_0000219098Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei101 – 1011PhosphotyrosineCombined sources
Disulfide bondi126 ↔ 149By similarity
Glycosylationi158 – 1581N-linked (GlcNAc...)1 Publication
Disulfide bondi159 ↔ 175By similarity
Glycosylationi163 – 1631N-linked (GlcNAc...); atypical1 Publication
Glycosylationi193 – 1931N-linked (GlcNAc...)By similarity
Disulfide bondi214 ↔ 277By similarity
Glycosylationi266 – 2661N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP14094.
PaxDbiP14094.
PRIDEiP14094.

PTM databases

iPTMnetiP14094.
PhosphoSiteiP14094.
SwissPalmiP14094.

Expressioni

Gene expression databases

BgeeiP14094.
CleanExiMM_ATP1B1.
MM_ATP4B.
ExpressionAtlasiP14094. baseline and differential.
GenevisibleiP14094. MM.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4 (By similarity). Interacts with MLC1. Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Interacts with KIRREL3 (By similarity).By similarity

GO - Molecular functioni

  • ATPase binding Source: MGI
  • MHC class II protein complex binding Source: MGI
  • protein C-terminus binding Source: MGI

Protein-protein interaction databases

BioGridi198244. 3 interactions.
IntActiP14094. 11 interactions.
MINTiMINT-4088581.
STRINGi10090.ENSMUSP00000027863.

Structurei

3D structure databases

ProteinModelPortaliP14094.
SMRiP14094. Positions 1-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 304114immunoglobulin-likeBy similarityAdd
BLAST

Domaini

The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3927. Eukaryota.
ENOG411150A. LUCA.
HOGENOMiHOG000039248.
HOVERGENiHBG050603.
InParanoidiP14094.
KOiK01540.
OMAiRGEFNNE.
OrthoDBiEOG7M6D85.
PhylomeDBiP14094.
TreeFamiTF314618.

Family and domain databases

InterProiIPR000402. Na/K_ATPase_sub_beta.
[Graphical view]
PfamiPF00287. Na_K-ATPase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITEiPS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF
60 70 80 90 100
IGTIQVMLLT ISELKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA
110 120 130 140 150
YVLNIIRFLE KYKDSAQKDD MIFEDCGNVP SEPKERGDIN HERGERKVCR
160 170 180 190 200
FKLDWLGNCS GLNDDSYGYR EGKPCIIIKL NRVLGFKPKP PKNESLETYP
210 220 230 240 250
LMMKYNPNVL PVQCTGKRDE DKDKVGNIEY FGMGGYYGFP LQYYPYYGKL
260 270 280 290 300
LQPKYLQPLL AVQFTNLTVD TEIRVECKAY GENIGYSEKD RFQGRFDVKI

EIKS
Length:304
Mass (Da):35,195
Last modified:January 1, 1990 - v1
Checksum:i8E15D8F860932680
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16646 mRNA. Translation: CAA34639.1.
BC027319 mRNA. Translation: AAH27319.1.
X61433 mRNA. Translation: CAA43675.1.
CCDSiCCDS35755.1.
PIRiS09601.
RefSeqiNP_033851.1. NM_009721.6.
UniGeneiMm.4550.

Genome annotation databases

EnsembliENSMUST00000027863; ENSMUSP00000027863; ENSMUSG00000026576.
GeneIDi11931.
KEGGimmu:11931.
UCSCiuc007dip.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16646 mRNA. Translation: CAA34639.1.
BC027319 mRNA. Translation: AAH27319.1.
X61433 mRNA. Translation: CAA43675.1.
CCDSiCCDS35755.1.
PIRiS09601.
RefSeqiNP_033851.1. NM_009721.6.
UniGeneiMm.4550.

3D structure databases

ProteinModelPortaliP14094.
SMRiP14094. Positions 1-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198244. 3 interactions.
IntActiP14094. 11 interactions.
MINTiMINT-4088581.
STRINGi10090.ENSMUSP00000027863.

PTM databases

iPTMnetiP14094.
PhosphoSiteiP14094.
SwissPalmiP14094.

Proteomic databases

EPDiP14094.
PaxDbiP14094.
PRIDEiP14094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027863; ENSMUSP00000027863; ENSMUSG00000026576.
GeneIDi11931.
KEGGimmu:11931.
UCSCiuc007dip.2. mouse.

Organism-specific databases

CTDi481.
MGIiMGI:88108. Atp1b1.

Phylogenomic databases

eggNOGiKOG3927. Eukaryota.
ENOG411150A. LUCA.
HOGENOMiHOG000039248.
HOVERGENiHBG050603.
InParanoidiP14094.
KOiK01540.
OMAiRGEFNNE.
OrthoDBiEOG7M6D85.
PhylomeDBiP14094.
TreeFamiTF314618.

Enzyme and pathway databases

ReactomeiR-MMU-210991. Basigin interactions.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiAtp1b1. mouse.
PROiP14094.
SOURCEiSearch...

Gene expression databases

BgeeiP14094.
CleanExiMM_ATP1B1.
MM_ATP4B.
ExpressionAtlasiP14094. baseline and differential.
GenevisibleiP14094. MM.

Family and domain databases

InterProiIPR000402. Na/K_ATPase_sub_beta.
[Graphical view]
PfamiPF00287. Na_K-ATPase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITEiPS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the mouse Na,K-ATPase beta-subunit."
    Gloor S.
    Nucleic Acids Res. 17:10117-10117(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary gland.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 15-21; 72-107; 171-179; 205-217; 225-249 AND 279-291, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "A collection of cDNA clones with specific expression patterns in mouse brain."
    Kato K.
    Eur. J. Neurosci. 2:704-711(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-304.
    Strain: BALB/cJ.
    Tissue: Brain.
  5. "A novel family of transmembrane proteins interacting with beta subunits of the Na,K-ATPase."
    Gorokhova S., Bibert S., Geering K., Heintz N.
    Hum. Mol. Genet. 16:2394-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NKAIN1; NKAIN2 AND NKAIN4.
    Strain: C57BL/6J.
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-158; ASN-163 AND ASN-266.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiAT1B1_MOUSE
AccessioniPrimary (citable) accession number: P14094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 8, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.