P14094 (AT1B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:	Atp1b1
Synonyms:	Atp4b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4. Ref.5
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	basolateral plasma membrane Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000219098
Regions
	Topological domain	1 – 34	34	Cytoplasmic Potential
	Transmembrane	35 – 62	28	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	63 – 304	242	Extracellular Potential
Amino acid modifications
	Modified residue	101	1	Phosphotyrosine Ref.6
	Glycosylation	158	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	163	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	193	1	N-linked (GlcNAc...) By similarity
	Glycosylation	266	1	N-linked (GlcNAc...) Ref.7
	Disulfide bond	126 ↔ 149		By similarity
	Disulfide bond	159 ↔ 175		By similarity
	Disulfide bond	214 ↔ 277		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14094 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 8E15D8F860932680	FASTA	304	35,195
	10 20 30 40 50 60 MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF IGTIQVMLLT 70 80 90 100 110 120 ISELKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA YVLNIIRFLE KYKDSAQKDD 130 140 150 160 170 180 MIFEDCGNVP SEPKERGDIN HERGERKVCR FKLDWLGNCS GLNDDSYGYR EGKPCIIIKL 190 200 210 220 230 240 NRVLGFKPKP PKNESLETYP LMMKYNPNVL PVQCTGKRDE DKDKVGNIEY FGMGGYYGFP 250 260 270 280 290 300 LQYYPYYGKL LQPKYLQPLL AVQFTNLTVD TEIRVECKAY GENIGYSEKD RFQGRFDVKI EIKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the mouse Na,K-ATPase beta-subunit." Gloor S. Nucleic Acids Res. 17:10117-10117(1989) [PubMed: 2557580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: 129. Tissue: Mammary gland.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 15-21; 72-107; 171-179; 205-217; 225-249 AND 279-291, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"A collection of cDNA clones with specific expression patterns in mouse brain." Kato K. Eur. J. Neurosci. 2:704-711(1990) [PubMed: 12106288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-304. Strain: BALB/c. Tissue: Brain.
[5]	"A novel family of transmembrane proteins interacting with beta subunits of the Na,K-ATPase." Gorokhova S., Bibert S., Geering K., Heintz N. Hum. Mol. Genet. 16:2394-2410(2007) [PubMed: 17606467] [Abstract] Cited for: INTERACTION WITH NKAIN1, NKAIN2, NKAIN4. Strain: C57BL/6.
[6]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-101, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-158; ASN-163 AND ASN-266, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16646 mRNA. Translation: CAA34639.1. BC027319 mRNA. Translation: AAH27319.1. X61433 mRNA. Translation: CAA43675.1.
IPI	IPI00121550.
PIR	S09601.
RefSeq	NP_033851.1. NM_009721.5.
UniGene	Mm.4550.
3D structure databases
ProteinModelPortal	P14094.
SMR	P14094. Positions 18-304.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14094. 5 interactions.
STRING	P14094.
PTM databases
PhosphoSite	P14094.
Proteomic databases
PRIDE	P14094.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027863; ENSMUSP00000027863; ENSMUSG00000026576.
GeneID	11931.
KEGG	mmu:11931.
Organism-specific databases
CTD	481.
MGI	MGI:88108. Atp1b1.
Phylogenomic databases
eggNOG	roNOG16210.
HOGENOM	HBG446583.
HOVERGEN	HBG050603.
InParanoid	P14094.
OMA	GFKPKPP.
OrthoDB	EOG4M3998.
PhylomeDB	P14094.
Gene expression databases
ArrayExpress	P14094.
Bgee	P14094.
CleanEx	MM_ATP1B1. MM_ATP4B.
Genevestigator	P14094.
GermOnline	ENSMUSG00000026576. Mus musculus.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. IPR015565. ATPase_P-typ_Na/K-dep_b1su. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit. PTHR11523:SF10. Na/K_ATPaseBeta1. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280017.
SOURCE	Search...

Entry information

Entry name

AT1B1_MOUSE

Accession

Primary (citable) accession number: P14094

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 16, 2011
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program