P14090 (GUNC_CELFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase C
EC=3.2.1.4

Alternative name(s):
Cellulase C
Endo-1,4-beta-glucanase C

Gene names

Name:	cenC
Ordered Locus Names:	Celf_1537

Organism

Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) [Complete proteome] [HAMAP]

Taxonomic identifier

590998 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Cellulomonadaceae › Cellulomonas › Cellulomonas fimi

Protein attributes

Sequence length	1101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 2 CBM-cenC (cenC-type cellulose-binding) domains. Contains 2 Ig-like (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Immunoglobulin domain Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Ref.1

Chain

33 – 1101

1069

Endoglucanase C

PRO_0000007946

Regions

Domain

64 – 173

110

CBM-cenC 1

Domain

212 – 318

107

CBM-cenC 2

Domain

918 – 1006

89

Ig-like 1

Domain

1008 – 1097

90

Ig-like 2

Region

329 – 880

552

Catalytic

Sites

Active site

831

1

By similarity

Active site

882

1

By similarity

Active site

891

1

By similarity

Secondary structure

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1101

Helix Strand Turn

Details...

Beta strand

42 – 44

3

Beta strand

49 – 52

4

Beta strand

62 – 68

7

Beta strand

76 – 84

9

Beta strand

91 – 102

12

Beta strand

107 – 114

8

Beta strand

119 – 123

5

Beta strand

132 – 139

8

Beta strand

155 – 160

6

Beta strand

169 – 180

12

Beta strand

184 – 186

3

Turn

190 – 192

3

Beta strand

198 – 203

6

Beta strand

212 – 215

4

Beta strand

226 – 230

5

Beta strand

239 – 251

13

Beta strand

253 – 260

8

Beta strand

262 – 265

4

Beta strand

272 – 277

6

Beta strand

282 – 289

8

Beta strand

296 – 299

4

Beta strand

303 – 307

5

Beta strand

315 – 324

10

Sequences

	Sequence		Length	Mass (Da)
	P14090 [UniParc]. Last modified March 1, 1992. Version 2. Checksum: 1FBAD189CC5F8B5D	FASTA	1,101	115,216
	10 20 30 40 50 60 MVSRRSSQAR GALTAVVATL ALALAGSGTA LAASPIGEGT FDDGPEGWVA YGTDGPLDTS 70 80 90 100 110 120 TGALCVAVPA GSAQYGVGVV LNGVAIEEGT TYTLRYTATA STDVTVRALV GQNGAPYGTV 130 140 150 160 170 180 LDTSPALTSE PRQVTETFTA SATYPATPAA DDPEGQIAFQ LGGFSADAWT FCLDDVALDS 190 200 210 220 230 240 EVELLPHTSF AESLGPWSLY GTSEPVFADG RMCVDLPGGQ GNPWDAGLVY NGVPVGEGES 250 260 270 280 290 300 YVLSFTASAT PDMPVRVLVG EGGGAYRTAF EQGSAPLTGE PATREYAFTS NLTFPPDGDA 310 320 330 340 350 360 PGQVAFHLGK AGAYEFCISQ VSLTTSATPP PGYEPDTGPR VRVNQVGYLP FGPKRATLVT 370 380 390 400 410 420 DAAEPVAWEL RDADGVVVAD GTSEPRGVEP SAAQAVHVLD FSDVTTQGAG YTLVADGETS 430 440 450 460 470 480 RPFDIDGDLY QQLRYDALNY FYLARSGTEI EADVVGEEYA REAGHVGVAP NQGDTDVPCI 490 500 510 520 530 540 GPRDYYDGWT CDYRLDVSGG WYDAGDHGKY VVNGGIAVGQ LLQTYERALH AGTADALADG 550 560 570 580 590 600 TLDVPEHGND VPDVLDEARW ELEWMLSMIV PEGEYAGMVH HKVHDEGWTG LPLLPADDPQ 610 620 630 640 650 660 ARSLHRPSTA ATLNLSAVAA QGARLLEPYD PQLAQTLLEA ARTTWAAAQE HPALYAPGEA 670 680 690 700 710 720 GADGGGAYND SQVADEFYWA AAELYLTTGE DAFATAVTTS PLHTADVFTA DGFGWGSVAA 730 740 750 760 770 780 LGRLDLATVP NELPGLDAVQ SSVVEGAQEY LAAQAGQGFG SLYSPPGGEY VWGSSSQVAN 790 800 810 820 830 840 NLVVVATAYD LTGDERFRAA TLEGLDYLFG RNALNQSYVT GWGEVASHQQ HSRWFAHQLD 850 860 870 880 890 900 PSLPSPPPGS LAGGPNSQAA TWDPTTKAAF PDGCAPSACY VDEIQAWSTN ELTVNWNSAL 910 920 930 940 950 960 SWVASWVADQ GSAEPVPTAP VVTRQPVDAT VALGADATFT AEASGVPAPT VRWQVRAGRG 970 980 990 1000 1010 1020 WKDVAGATGT TLTVRATART DGTRYRAVFT NAAGSVESAV VRLTVERAAP VVTQHPADVR 1030 1040 1050 1060 1070 1080 ARVGTRAVFR AAADGYPTPC VVWQVRWGGG SWRPIPWATS TTLSVPVTVL AAGTEYRAVF 1090 1100 TNAVGTAATE PAELAVQRPR S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products." Coutinho J.B., Moser B., Kilburn D.G., Warren R.A.J., Miller R.C. Jr. Mol. Microbiol. 5:1221-1233(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 33-42. Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[2]	"Complete sequence of Cellulomonas fimi ATCC 484." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T. Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[3]	"Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene." Moser B., Gilkes N.R., Kilburn D.G., Warren R.A.J., Miller R.C. Jr. Appl. Environ. Microbiol. 55:2480-2487(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64, PROTEIN SEQUENCE OF 625-641. Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[4]	"The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats." Coutinho J.B., Gilkes N.R., Warren R.A.J., Kilburn D.G., Miller R.C. Jr. Mol. Microbiol. 6:1243-1252(1992) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAINS CELLULOSE BINDING.
[5]	"Members of the immunoglobulin superfamily in bacteria." Bateman A., Eddy S.R., Chothia C. Protein Sci. 5:1939-1942(1996) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAINS IG-LIKE.
[6]	"Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by nuclear magnetic resonance spectroscopy." Johnson P.E., Joshi M.D., Tomme P., Kilburn D.G., McIntosh L.P. Biochemistry 35:14381-14394(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 33-184.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X57858 Genomic DNA. Translation: CAA40993.1.
CP002666 Genomic DNA. Translation: AEE45671.1.
M29707 Genomic DNA. Translation: AAA23087.1. Different termination.
M29708 Genomic DNA. Translation: AAA23088.1. Sequence problems.

PIR

S15271.

RefSeq

YP_004453058.1. NC_015514.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CX1	NMR	-	A	178-328	[»]
1GU3	X-ray	2.30	A	33-181	[»]
1ULO	NMR	-	A	33-184	[»]
1ULP	NMR	-	A	33-184	[»]

ProteinModelPortal

P14090.

SMR

P14090. Positions 33-328.

ModBase

Search...

Protein family/group databases

CAZy

CBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AEE45671; AEE45671; Celf_1537.

GeneID

10592314.

KEGG

cfi:Celf_1537.

PATRIC

54613973. VBICelFim18861_1533.

Organism-specific databases

CMR

Search...

Enzyme and pathway databases

BioCyc

CFIM590998:GJFK-1554-MONOMER.

Family and domain databases

Gene3D

1.50.10.10. 1 hit.
2.60.40.10. 3 hits.

InterPro

IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR003305. CenC_carb-bd.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view]

Pfam

PF02018. CBM_4_9. 2 hits.
PF02927. CelD_N. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 2 hits.
SSF48208. Glyco_trans_6hp. 1 hit.
SSF81296. Ig_E-set. 1 hit.

PROSITE

PS00592. GLYCOSYL_HYDROL_F9_1. False negative.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14090.

Entry information

Entry name

GUNC_CELFA

Accession

Primary (citable) accession number: P14090
Secondary accession number(s): F4H737

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program