P14080 (PAPA2_CARPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Chymopapain EC=3.4.22.6 Alternative name(s): Papaya proteinase II Short name=PPII
Organism	Carica papaya (Papaya)
Taxonomic identifier	3649 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Caricaceae › Carica

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Specificity similar to that of papain.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Zymogen
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Potential

Propeptide

19 – 134

116

Activation peptide

PRO_0000026408

Chain

135 – 352

218

Chymopapain

PRO_0000026409

Sites

Active site

159

1

Active site

293

1

Active site

313

1

Amino acid modifications

Disulfide bond

156 ↔ 197

Disulfide bond

190 ↔ 229

Disulfide bond

287 ↔ 338

Secondary structure

1

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352

Helix Strand Turn

Details...

Turn

141 – 145

5

Beta strand

155 – 157

3

Helix

159 – 176

18

Helix

184 – 190

7

Helix

202 – 212

11

Turn

217 – 219

3

Helix

231 – 233

3

Beta strand

242 – 246

5

Beta strand

249 – 251

3

Helix

252 – 259

8

Beta strand

264 – 268

5

Helix

273 – 276

4

Beta strand

280 – 283

4

Beta strand

293 – 303

11

Beta strand

306 – 312

7

Beta strand

324 – 328

5

Beta strand

332 – 334

3

Helix

337 – 339

3

Beta strand

345 – 348

4

Sequences

	Sequence		Length	Mass (Da)
	P14080 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 50EA31EBFCF0AF9F	FASTA	352	39,415
	10 20 30 40 50 60 MATMSSISKI IFLATCLIIH MGLSSADFYT VGYSQDDLTS IERLIQLFDS WMLKHNKIYE 70 80 90 100 110 120 SIDEKIYRFE IFRDNLMYID ETNKKNNSYW LGLNGFADLS NDEFKKKYVG FVAEDFTGLE 130 140 150 160 170 180 HFDNEDFTYK HVTNYPQSID WRAKGAVTPV KNQGACGSCW AFSTIATVEG INKIVTGNLL 190 200 210 220 230 240 ELSEQELVDC DKHSYGCKGG YQTTSLQYVA NNGVHTSKVY PYQAKQYKCR ATDKPGPKVK 250 260 270 280 290 300 ITGYKRVPSN CETSFLGALA NQPLSVLVEA GGKPFQLYKS GVFDGPCGTK LDHAVTAVGY 310 320 330 340 350 GTSDGKNYII IKNSWGPNWG EKGYMRLKRQ SGNSQGTCGV YKSSYYPFKG FA

References

[1]	Connerton I.F. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"The amino acid sequence of chymopapain from Carica papaya." Watson D.C., Yaguchi M., Lynn K.R. Biochem. J. 266:75-81(1990) [PubMed: 2106878] [Abstract] Cited for: PROTEIN SEQUENCE OF 135-352.
[3]	"The thiol proteinases from the latex of Carica papaya L. III. The primary structure of chymopapain." Jacquet A., Kleinschmidt T., Schnek A.G., Looze Y., Braunitzer G. Biol. Chem. Hoppe-Seyler 370:425-434(1989) [PubMed: 2500950] [Abstract] Cited for: PROTEIN SEQUENCE OF 135-352.
[4]	"Structure of chymopapain at 1.7-A resolution." Maes D., Bouckaert J., Poortmans F., Wyns L., Looze Y. Biochemistry 35:16292-16298(1996) [PubMed: 8973203] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X97789 mRNA. Translation: CAA66378.1.

PIR

T09760.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YAL	X-ray	1.70	A	135-352	[»]

ProteinModelPortal

P14080.

SMR

P14080. Positions 38-352.

ModBase

Search...

Protein family/group databases

Allergome

1540. Car p Chymopapain.

MEROPS

C01.002.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000169. Pept_cys_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]

PANTHER

PTHR12411. Peptidase_C1A. 1 hit.

Pfam

PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

SMART

SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PAPA2_CARPA

Accession

Primary (citable) accession number: P14080

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program