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P14077

- GAG_HTL1M

UniProt

P14077 - GAG_HTL1M

Protein

Gag polyprotein

Gene

gag

Organism
Human T-cell leukemia virus 1 (strain Japan MT-2 subtype A) (HTLV-1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Matrix protein p19 targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.By similarity
    Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.By similarity
    Nucleocapsid protein p15 is involved in the packaging and encapsidation of two copies of the genome.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei130 – 1312Cleavage; by viral proteaseBy similarity
    Sitei344 – 3452Cleavage; by viral proteaseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri355 – 37218CCHC-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri378 – 39518CCHC-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. structural molecule activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    Metal-binding, Viral nucleoprotein, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gag polyprotein
    Alternative name(s):
    Pr53Gag
    Cleaved into the following 3 chains:
    Matrix protein p19
    Short name:
    MA
    Capsid protein p24
    Short name:
    CA
    Gene namesi
    Name:gag
    OrganismiHuman T-cell leukemia virus 1 (strain Japan MT-2 subtype A) (HTLV-1)
    Taxonomic identifieri11928 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeDeltaretrovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    GO - Cellular componenti

    1. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by host
    Chaini2 – 429428Gag polyproteinPRO_0000259773Add
    BLAST
    Chaini2 – 130129Matrix protein p19By similarityPRO_0000038817Add
    BLAST
    Chaini131 – 344214Capsid protein p24By similarityPRO_0000038818Add
    BLAST
    Chaini345 – 42985Nucleocapsid protein p15-gagBy similarityPRO_0000038819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by host1 Publication
    Modified residuei105 – 1051Phosphoserine; by host MAPK1By similarity

    Post-translational modificationi

    Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.By similarity
    Phosphorylation of the matrix protein p19 by MAPK1 seems to play a role in budding.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with human TSG101 and NEDD4. These interactions are essential for budding and release of viral particles By similarity.By similarity

    Structurei

    Secondary structure

    1
    429
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi136 – 1383
    Helixi148 – 15912
    Beta strandi160 – 1634
    Helixi166 – 17712
    Helixi182 – 1909
    Helixi195 – 21622
    Beta strandi217 – 2193
    Helixi227 – 2315
    Helixi238 – 25215
    Turni254 – 2574
    Turni261 – 2633

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G03NMR-A131-264[»]
    ProteinModelPortaliP14077.
    SMRiP14077. Positions 1-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14077.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi118 – 1214PPXY motif
    Motifi124 – 1274PTAP/PSAP motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi95 – 14450Pro-richAdd
    BLAST

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Matrix protein p19 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of HECT (homologous to E6-AP C-terminus) E3 ubiquitin ligases, like NEDD4 By similarity.By similarity
    The capsid protein N-terminus seems to be involved in Gag-Gag interactions.By similarity

    Sequence similaritiesi

    Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri355 – 37218CCHC-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri378 – 39518CCHC-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Family and domain databases

    Gene3Di1.10.1200.30. 1 hit.
    1.10.185.10. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProiIPR003139. D_retro_matrix_N.
    IPR000721. Gag_p24.
    IPR008916. Retrov_capsid_C.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF02228. Gag_p19. 1 hit.
    PF00607. Gag_p24. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 2 hits.
    [Graphical view]
    SUPFAMiSSF47353. SSF47353. 1 hit.
    SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEiPS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by ribosomal frameshifting. Align

    Note: This strategy of translation probably allows the virus to modulate the quantity of each viral protein.

    Isoform Gag polyprotein (identifier: P14077-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGQIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL    50
    KIALETPVWI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAP 100
    PPPSSPTHDP PDSDPQIPPP YVEPTAPQVL PVMHPHGAPP NHRPWQMKDL 150
    QAIKQEVSQA APGSPQFMQT IRLAVQQFDP TAKDLQDLLQ YLCSSLVASL 200
    HHQQLDSLIS EAETRGITSY NPLAGPLRVQ ANNPQQQGLR REYQQLWLAA 250
    FAALPGSAKD PSWASILQGL EEPYHAFVER LNIALDNGLP EGTPKDPILR 300
    SLAYSNANKE CQKLLQARGH TNSPLGDMLR ACQTWTPKDK TKVLVVQPKK 350
    PPPNQPCFRC GKAGHWSRDC TQPRPPPGPC PLCQDPTHWK RDCPRLKPTI 400
    PEPEPEEDAL LLDLPADIPH PKNSIGGEV 429

    Note: Produced by conventional translation.

    Length:429
    Mass (Da):47,585
    Last modified:January 23, 2007 - v3
    Checksum:iEF5201C934EF0291
    GO
    Isoform Gag-Pro polyprotein (identifier: P14077-2)

    Sequence is not available

    Note: Produced by -1 ribosomal frameshifting at the gag-pro genes boundary.

    Length:
    Mass (Da):
    Isoform Gag-Pol polyprotein (identifier: P14077-3)

    Sequence is not available

    Note: Produced by -1 ribosomal frameshifting at the gag-pol genes boundary.

    Length:
    Mass (Da):

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15951 Genomic RNA. Translation: CAA34075.1.
    PIRiS06073.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15951 Genomic RNA. Translation: CAA34075.1 .
    PIRi S06073.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G03 NMR - A 131-264 [» ]
    ProteinModelPortali P14077.
    SMRi P14077. Positions 1-344.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P14077.

    Family and domain databases

    Gene3Di 1.10.1200.30. 1 hit.
    1.10.185.10. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProi IPR003139. D_retro_matrix_N.
    IPR000721. Gag_p24.
    IPR008916. Retrov_capsid_C.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF02228. Gag_p19. 1 hit.
    PF00607. Gag_p24. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47353. SSF47353. 1 hit.
    SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEi PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the core (gag) gene from HTLV-1 isolate MT-2."
      Gray G.S., Bartman T., White M.
      Nucleic Acids Res. 17:7998-7998(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Antibodies to an NH2-terminal myristoyl glycine moiety can detect NH2-terminal myristoylated proteins in the retrovirus-infected cells."
      Shoji S., Tashiro A., Furuishi K., Takenaka O., Kida Y., Horiuchi S., Funakoshi T., Kubota Y.
      Biochem. Biophys. Res. Commun. 162:724-732(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: MYRISTOYLATION AT GLY-2.
    3. "Structural analysis of the N-terminal domain of the human T-cell leukemia virus capsid protein."
      Cornilescu C.C., Bouamr F., Yao X., Carter C., Tjandra N.
      J. Mol. Biol. 306:783-797(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 131-264.

    Entry informationi

    Entry nameiGAG_HTL1M
    AccessioniPrimary (citable) accession number: P14077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    HTLV-1 lineages are divided in four clades, A (Cosmopolitan), B (Central African group), C (Melanesian group) and D (New Central African group).

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3