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P14069

- S10A6_MOUSE

UniProt

P14069 - S10A6_MOUSE

Protein

Protein S100-A6

Gene

S100a6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi20 – 33141PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi61 – 72122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. ion transmembrane transporter activity Source: Ensembl
    3. protein binding Source: IntAct
    4. zinc ion binding Source: UniProtKB

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein S100-A6
    Alternative name(s):
    5B10
    Calcyclin
    Prolactin receptor-associated protein
    S100 calcium-binding protein A6
    Gene namesi
    Name:S100a6
    Synonyms:Cacy
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1339467. S100a6.

    Subcellular locationi

    Nucleus envelope By similarity. Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
    3. nuclear envelope Source: UniProtKB-SubCell
    4. perinuclear region of cytoplasm Source: Ensembl
    5. ruffle Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8989Protein S100-A6PRO_0000143985Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401N6-acetyllysineBy similarity
    Modified residuei47 – 471N6-acetyllysine; alternate1 Publication
    Modified residuei47 – 471N6-succinyllysine; alternate1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP14069.
    PaxDbiP14069.
    PRIDEiP14069.

    2D gel databases

    DOSAC-COBS-2DPAGEP14069.

    PTM databases

    PhosphoSiteiP14069.

    Expressioni

    Gene expression databases

    ArrayExpressiP14069.
    BgeeiP14069.
    CleanExiMM_S100A6.
    GenevestigatoriP14069.

    Interactioni

    Subunit structurei

    Homodimer; head to tail assembly of 2 subunits. Interacts with CACYBP in a calcium-dependent manner. Interacts with ANXA2 and ANXA11 (via N-terminus). Interacts with SUGT1. Interacts with TP53; has higher affinity for TP53 that is phosphorylated on its N-terminal domain, and lower affinity for TP53 that is phosphorylated on its C-terminal domain. Interacts with tropomyosin. Interacts with FKBP4. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CacybpQ9CXW34EBI-6478740,EBI-767146

    Protein-protein interaction databases

    BioGridi203055. 2 interactions.
    IntActiP14069. 4 interactions.
    MINTiMINT-239145.
    STRINGi10090.ENSMUSP00000001051.

    Structurei

    3D structure databases

    ProteinModelPortaliP14069.
    SMRiP14069. Positions 2-89.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 4736EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini48 – 8336EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the S-100 family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG40006.
    HOGENOMiHOG000246968.
    HOVERGENiHBG001479.
    InParanoidiP14069.
    OMAiNIFERDY.
    OrthoDBiEOG78WKVD.
    PhylomeDBiP14069.
    TreeFamiTF332727.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view]
    PfamiPF01023. S_100. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14069-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACPLDQAIG LLVAIFHKYS GKEGDKHTLS KKELKELIQK ELTIGSKLQD   50
    AEIARLMDDL DRNKDQEVNF QEYVAFLGAL ALIYNEALK 89
    Length:89
    Mass (Da):10,051
    Last modified:May 1, 1991 - v3
    Checksum:i2AA1A4018B8CA687
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511A → R in CAA36521. (PubMed:2177633)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37761 mRNA. Translation: AAA37358.1.
    X66449 mRNA. Translation: CAA47065.1.
    X52278 mRNA. Translation: CAA36521.1.
    BC003832 mRNA. Translation: AAH03832.1.
    BC010774 mRNA. Translation: AAH10774.1.
    CCDSiCCDS38506.1.
    PIRiA54314.
    RefSeqiNP_035443.1. NM_011313.2.
    UniGeneiMm.100144.

    Genome annotation databases

    EnsembliENSMUST00000001051; ENSMUSP00000001051; ENSMUSG00000001025.
    GeneIDi20200.
    KEGGimmu:20200.
    UCSCiuc008qdb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37761 mRNA. Translation: AAA37358.1 .
    X66449 mRNA. Translation: CAA47065.1 .
    X52278 mRNA. Translation: CAA36521.1 .
    BC003832 mRNA. Translation: AAH03832.1 .
    BC010774 mRNA. Translation: AAH10774.1 .
    CCDSi CCDS38506.1.
    PIRi A54314.
    RefSeqi NP_035443.1. NM_011313.2.
    UniGenei Mm.100144.

    3D structure databases

    ProteinModelPortali P14069.
    SMRi P14069. Positions 2-89.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203055. 2 interactions.
    IntActi P14069. 4 interactions.
    MINTi MINT-239145.
    STRINGi 10090.ENSMUSP00000001051.

    PTM databases

    PhosphoSitei P14069.

    2D gel databases

    DOSAC-COBS-2DPAGE P14069.

    Proteomic databases

    MaxQBi P14069.
    PaxDbi P14069.
    PRIDEi P14069.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001051 ; ENSMUSP00000001051 ; ENSMUSG00000001025 .
    GeneIDi 20200.
    KEGGi mmu:20200.
    UCSCi uc008qdb.1. mouse.

    Organism-specific databases

    CTDi 6277.
    MGIi MGI:1339467. S100a6.

    Phylogenomic databases

    eggNOGi NOG40006.
    HOGENOMi HOG000246968.
    HOVERGENi HBG001479.
    InParanoidi P14069.
    OMAi NIFERDY.
    OrthoDBi EOG78WKVD.
    PhylomeDBi P14069.
    TreeFami TF332727.

    Miscellaneous databases

    ChiTaRSi S100A6. mouse.
    NextBioi 297775.
    PROi P14069.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P14069.
    Bgeei P14069.
    CleanExi MM_S100A6.
    Genevestigatori P14069.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view ]
    Pfami PF01023. S_100. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene encoding the calcium binding protein calcyclin is expressed at sites of exocytosis in the mouse."
      Timmons P.M., Chan C.T.J., Rigby P.W.J., Poirier F.
      J. Cell Sci. 104:187-196(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129.
    2. "Identification of a serum-inducible messenger RNA (5B10) as the mouse homologue of calcyclin: tissue distribution and expression in metastatic, ras-transformed NIH 3T3 cells."
      Guo X., Chambers A.F., Parfett C.L., Waterhouse P., Murphy L.C., Reid R.E., Craig A.M., Edwards D.R., Denhardt D.T.
      Cell Growth Differ. 1:333-338(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Expression of calcyclin, a calcium-binding protein, in the keratogenous region of growing hair follicles."
      Wood L., Carter D., Mills M., Hatzenbuhler N., Vogeli G.
      J. Invest. Dermatol. 96:383-387(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: TUC CF1.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon and Mammary tumor.
    5. "Calcium-binding protein from mouse Ehrlich ascites-tumour cells is homologous to human calcyclin."
      Kuznicki J., Filipek A., Hunziker P.E., Huber S., Heizmann C.W.
      Biochem. J. 263:951-956(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 56-69.
    6. "Characterization of the cell-cycle-regulated protein calcyclin from Ehrlich ascites tumor cells. Identification of two binding proteins obtained by Ca2(+)-dependent affinity chromatography."
      Filipek A., Gerke V., Weber K., Kuznicki J.
      Eur. J. Biochem. 195:795-800(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 6-89, INTERACTION WITH OTHER PROTEINS.
    7. "Purification and characterization of mouse decidual calcyclin: a novel stimulator of mouse placental lactogen-II secretion."
      Thordarson G., Southard J.N., Talamantes F.
      Endocrinology 129:1257-1265(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-33 AND 37-89.
    8. "CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family."
      Filipek A., Jastrzebska B., Nowotny M., Kuznicki J.
      J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CACYBP.
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-47, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiS10A6_MOUSE
    AccessioniPrimary (citable) accession number: P14069
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3