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Protein

Protein S100-A6

Gene

S100a6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi20 – 33141PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi61 – 72122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent protein binding Source: MGI
  2. calcium ion binding Source: UniProtKB
  3. ion transmembrane transporter activity Source: Ensembl
  4. protein homodimerization activity Source: MGI
  5. S100 protein binding Source: MGI
  6. tropomyosin binding Source: MGI
  7. zinc ion binding Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-A6
Alternative name(s):
5B10
Calcyclin
Prolactin receptor-associated protein
S100 calcium-binding protein A6
Gene namesi
Name:S100a6
Synonyms:Cacy
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1339467. S100a6.

Subcellular locationi

Nucleus envelope By similarity. Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: UniProtKB
  3. extracellular vesicular exosome Source: MGI
  4. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  5. nuclear envelope Source: MGI
  6. nucleus Source: MGI
  7. perinuclear region of cytoplasm Source: MGI
  8. ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8989Protein S100-A6PRO_0000143985Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401N6-acetyllysineBy similarity
Modified residuei47 – 471N6-acetyllysine; alternate1 Publication
Modified residuei47 – 471N6-succinyllysine; alternate1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP14069.
PaxDbiP14069.
PRIDEiP14069.

2D gel databases

DOSAC-COBS-2DPAGEP14069.

PTM databases

PhosphoSiteiP14069.

Expressioni

Gene expression databases

BgeeiP14069.
CleanExiMM_S100A6.
ExpressionAtlasiP14069. baseline and differential.
GenevestigatoriP14069.

Interactioni

Subunit structurei

Homodimer; head to tail assembly of 2 subunits. Interacts with CACYBP in a calcium-dependent manner. Interacts with ANXA2 and ANXA11 (via N-terminus). Interacts with SUGT1. Interacts with TP53; has higher affinity for TP53 that is phosphorylated on its N-terminal domain, and lower affinity for TP53 that is phosphorylated on its C-terminal domain. Interacts with tropomyosin. Interacts with FKBP4. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CacybpQ9CXW34EBI-6478740,EBI-767146

Protein-protein interaction databases

BioGridi203055. 2 interactions.
IntActiP14069. 4 interactions.
MINTiMINT-239145.
STRINGi10090.ENSMUSP00000001051.

Structurei

3D structure databases

ProteinModelPortaliP14069.
SMRiP14069. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 4736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini48 – 8336EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the S-100 family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG40006.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP14069.
OMAiQVVNFQE.
OrthoDBiEOG78WKVD.
PhylomeDBiP14069.
TreeFamiTF332727.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PfamiPF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14069-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACPLDQAIG LLVAIFHKYS GKEGDKHTLS KKELKELIQK ELTIGSKLQD
60 70 80
AEIARLMDDL DRNKDQEVNF QEYVAFLGAL ALIYNEALK
Length:89
Mass (Da):10,051
Last modified:May 1, 1991 - v3
Checksum:i2AA1A4018B8CA687
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511A → R in CAA36521. (PubMed:2177633)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37761 mRNA. Translation: AAA37358.1.
X66449 mRNA. Translation: CAA47065.1.
X52278 mRNA. Translation: CAA36521.1.
BC003832 mRNA. Translation: AAH03832.1.
BC010774 mRNA. Translation: AAH10774.1.
CCDSiCCDS38506.1.
PIRiA54314.
RefSeqiNP_035443.1. NM_011313.2.
UniGeneiMm.100144.

Genome annotation databases

EnsembliENSMUST00000001051; ENSMUSP00000001051; ENSMUSG00000001025.
GeneIDi20200.
KEGGimmu:20200.
UCSCiuc008qdb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37761 mRNA. Translation: AAA37358.1.
X66449 mRNA. Translation: CAA47065.1.
X52278 mRNA. Translation: CAA36521.1.
BC003832 mRNA. Translation: AAH03832.1.
BC010774 mRNA. Translation: AAH10774.1.
CCDSiCCDS38506.1.
PIRiA54314.
RefSeqiNP_035443.1. NM_011313.2.
UniGeneiMm.100144.

3D structure databases

ProteinModelPortaliP14069.
SMRiP14069. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203055. 2 interactions.
IntActiP14069. 4 interactions.
MINTiMINT-239145.
STRINGi10090.ENSMUSP00000001051.

PTM databases

PhosphoSiteiP14069.

2D gel databases

DOSAC-COBS-2DPAGEP14069.

Proteomic databases

MaxQBiP14069.
PaxDbiP14069.
PRIDEiP14069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001051; ENSMUSP00000001051; ENSMUSG00000001025.
GeneIDi20200.
KEGGimmu:20200.
UCSCiuc008qdb.1. mouse.

Organism-specific databases

CTDi6277.
MGIiMGI:1339467. S100a6.

Phylogenomic databases

eggNOGiNOG40006.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP14069.
OMAiQVVNFQE.
OrthoDBiEOG78WKVD.
PhylomeDBiP14069.
TreeFamiTF332727.

Miscellaneous databases

ChiTaRSiS100a6. mouse.
NextBioi297775.
PROiP14069.
SOURCEiSearch...

Gene expression databases

BgeeiP14069.
CleanExiMM_S100A6.
ExpressionAtlasiP14069. baseline and differential.
GenevestigatoriP14069.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PfamiPF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding the calcium binding protein calcyclin is expressed at sites of exocytosis in the mouse."
    Timmons P.M., Chan C.T.J., Rigby P.W.J., Poirier F.
    J. Cell Sci. 104:187-196(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129.
  2. "Identification of a serum-inducible messenger RNA (5B10) as the mouse homologue of calcyclin: tissue distribution and expression in metastatic, ras-transformed NIH 3T3 cells."
    Guo X., Chambers A.F., Parfett C.L., Waterhouse P., Murphy L.C., Reid R.E., Craig A.M., Edwards D.R., Denhardt D.T.
    Cell Growth Differ. 1:333-338(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression of calcyclin, a calcium-binding protein, in the keratogenous region of growing hair follicles."
    Wood L., Carter D., Mills M., Hatzenbuhler N., Vogeli G.
    J. Invest. Dermatol. 96:383-387(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: TUC CF1.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon and Mammary tumor.
  5. "Calcium-binding protein from mouse Ehrlich ascites-tumour cells is homologous to human calcyclin."
    Kuznicki J., Filipek A., Hunziker P.E., Huber S., Heizmann C.W.
    Biochem. J. 263:951-956(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 56-69.
  6. "Characterization of the cell-cycle-regulated protein calcyclin from Ehrlich ascites tumor cells. Identification of two binding proteins obtained by Ca2(+)-dependent affinity chromatography."
    Filipek A., Gerke V., Weber K., Kuznicki J.
    Eur. J. Biochem. 195:795-800(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 6-89, INTERACTION WITH OTHER PROTEINS.
  7. "Purification and characterization of mouse decidual calcyclin: a novel stimulator of mouse placental lactogen-II secretion."
    Thordarson G., Southard J.N., Talamantes F.
    Endocrinology 129:1257-1265(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-33 AND 37-89.
  8. "CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family."
    Filipek A., Jastrzebska B., Nowotny M., Kuznicki J.
    J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CACYBP.
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-47, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiS10A6_MOUSE
AccessioniPrimary (citable) accession number: P14069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 1, 1991
Last modified: February 4, 2015
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.