P14069 (S10A6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A6

Alternative name(s):
5B10
Calcyclin
Prolactin receptor-associated protein
S100 calcium-binding protein A6

Gene names

Name:	S100a6
Synonyms:	Cacy

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as calcium sensor and contribute to cellular calcium signaling Potential. May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative By similarity. Interacts with FKBP4 By similarity.
Subunit structure	Homodimer; head to tail assembly of 2 subunits. Interacts with ANXA2 and ANXA11 (via N-terminus). Interacts with SUGT1 and CACYBP. Interacts with TP53. Interacts with tropomyosin By similarity. Interacts with CACYBP in a calcium-dependent manner. Ref.6 Ref.8
Subcellular location	Nucleus envelope By similarity. Cytoplasm By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB extrinsic to internal side of plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara ruffle Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from direct assay PubMed 2358072. Source: UniProtKB ion transmembrane transporter activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from direct assay PubMed 2358072. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cacybp	Q9CXW3	4	EBI-6478740,EBI-767146

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 89	89	Protein S100-A6		PRO_0000143985
Regions
	Domain	12 – 47	36	EF-hand 1
	Domain	48 – 83	36	EF-hand 2
	Calcium binding	20 – 33	14	1 By similarity
	Calcium binding	61 – 72	12	2 By similarity
Amino acid modifications
	Modified residue	40	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	51	1	A → R in CAA36521. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14069 [UniParc]. Last modified May 1, 1991. Version 3. Checksum: 2AA1A4018B8CA687	FASTA	89	10,051
	10 20 30 40 50 60 MACPLDQAIG LLVAIFHKYS GKEGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMDDL 70 80 DRNKDQEVNF QEYVAFLGAL ALIYNEALK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene encoding the calcium binding protein calcyclin is expressed at sites of exocytosis in the mouse." Timmons P.M., Chan C.T.J., Rigby P.W.J., Poirier F. J. Cell Sci. 104:187-196(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129.
[2]	"Identification of a serum-inducible messenger RNA (5B10) as the mouse homologue of calcyclin: tissue distribution and expression in metastatic, ras-transformed NIH 3T3 cells." Guo X., Chambers A.F., Parfett C.L., Waterhouse P., Murphy L.C., Reid R.E., Craig A.M., Edwards D.R., Denhardt D.T. Cell Growth Differ. 1:333-338(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of calcyclin, a calcium-binding protein, in the keratogenous region of growing hair follicles." Wood L., Carter D., Mills M., Hatzenbuhler N., Vogeli G. J. Invest. Dermatol. 96:383-387(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: TUC CF1.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Mammary tumor.
[5]	"Calcium-binding protein from mouse Ehrlich ascites-tumour cells is homologous to human calcyclin." Kuznicki J., Filipek A., Hunziker P.E., Huber S., Heizmann C.W. Biochem. J. 263:951-956(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 56-69.
[6]	"Characterization of the cell-cycle-regulated protein calcyclin from Ehrlich ascites tumor cells. Identification of two binding proteins obtained by Ca2(+)-dependent affinity chromatography." Filipek A., Gerke V., Weber K., Kuznicki J. Eur. J. Biochem. 195:795-800(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 6-89, INTERACTION WITH OTHER PROTEINS.
[7]	"Purification and characterization of mouse decidual calcyclin: a novel stimulator of mouse placental lactogen-II secretion." Thordarson G., Southard J.N., Talamantes F. Endocrinology 129:1257-1265(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-33 AND 37-89.
[8]	"CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family." Filipek A., Jastrzebska B., Nowotny M., Kuznicki J. J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CACYBP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37761 mRNA. Translation: AAA37358.1. X66449 mRNA. Translation: CAA47065.1. X52278 mRNA. Translation: CAA36521.1. BC003832 mRNA. Translation: AAH03832.1. BC010774 mRNA. Translation: AAH10774.1.
IPI	IPI00121427.
PIR	A54314.
RefSeq	NP_035443.1. NM_011313.2.
UniGene	Mm.100144.
3D structure databases
ProteinModelPortal	P14069.
SMR	P14069. Positions 2-89.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14069. 1 interaction.
STRING	10090.ENSMUSP00000001051.
PTM databases
PhosphoSite	P14069.
2D gel databases
DOSAC-COBS-2DPAGE	P14069.
Proteomic databases
PaxDb	P14069.
PRIDE	P14069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000001051; ENSMUSP00000001051; ENSMUSG00000001025.
GeneID	20200.
KEGG	mmu:20200.
Organism-specific databases
CTD	6277.
MGI	MGI:1339467. S100a6.
Phylogenomic databases
eggNOG	NOG40006.
HOGENOM	HOG000246968.
HOVERGEN	HBG001479.
InParanoid	P14069.
OMA	VVNFQEY.
OrthoDB	EOG4MSD0W.
Gene expression databases
ArrayExpress	P14069.
Bgee	P14069.
CleanEx	MM_S100A6.
Genevestigator	P14069.
GermOnline	ENSMUSG00000001025. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR001751. S100/CaBP-9k_CS. IPR013787. S100_Ca-bd_sub. [Graphical view]
Pfam	PF01023. S_100. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS00303. S100_CABP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	S100A6. mouse.
NextBio	297775.
SOURCE	Search...

Entry information

Entry name

S10A6_MOUSE

Accession

Primary (citable) accession number: P14069

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	May 1, 1991
Last modified:	May 1, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program