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P14068

- XPO1_SCHPO

UniProt

P14068 - XPO1_SCHPO

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Protein
Exportin-1
Gene
xpo1, caf2, crm1, SPAC1805.17, SPAC1B2.01
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for the leucine-rich nuclear export signal (NES).1 Publication

GO - Molecular functioni

  1. GTP binding Source: PomBase
  2. nuclear export signal receptor activity Source: PomBase

GO - Biological processi

  1. cellular response to oxidative stress Source: PomBase
  2. negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  3. protein export from nucleus Source: PomBase
  4. protein targeting Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_199398. Cyclin A/B1 associated events during G2/M transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Exportin-1
Alternative name(s):
Caffeine resistance protein 2
Chromosome region maintenance protein 1
Gene namesi
Name:xpo1
Synonyms:caf2, crm1
ORF Names:SPAC1805.17, SPAC1B2.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC1805.17.

Subcellular locationi

Nucleus
Note: Localized in the nucleus and at its periphery.

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. nuclear envelope Source: PomBase
  3. nuclear periphery Source: PomBase
  4. nuclear pore Source: PomBase
  5. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi129 – 1291E → K in crm1-1R-9R. 1 Publication
Mutagenesisi299 – 2991E → K in crm1-14-R. 1 Publication
Mutagenesisi430 – 4301E → K in crm1-809; cold-sensitive and LMB hypersensitive. 1 Publication
Mutagenesisi503 – 5031G → D in crm1-N1. 1 Publication
Mutagenesisi529 – 5291C → S in crm1-K1; LMB resistant. 1 Publication
Mutagenesisi546 – 5461M → I in crm1-N1. 1 Publication
Mutagenesisi992 – 9921F → S in crm1-119; LMB resistant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10781078Exportin-1
PRO_0000204709Add
BLAST

Proteomic databases

MaxQBiP14068.

Interactioni

Subunit structurei

Interacts with php4.1 Publication

Protein-protein interaction databases

BioGridi280563. 11 interactions.
MINTiMINT-4687292.
STRINGi4896.SPAC1805.17-1.

Structurei

3D structure databases

ProteinModelPortaliP14068.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 10067Importin N-terminal
Add
BLAST

Sequence similaritiesi

Belongs to the exportin family.

Phylogenomic databases

eggNOGiCOG5101.
KOiK14290.
OMAiILKQAWP.
OrthoDBiEOG7MWH56.
PhylomeDBiP14068.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR014877. CRM1_C_dom.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF08767. CRM1_C. 1 hit.
PF03810. IBN_N. 1 hit.
PF08389. Xpo1. 1 hit.
[Graphical view]
SMARTiSM01102. CRM1_C. 1 hit.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14068-1 [UniParc]FASTAAdd to Basket

« Hide

MEGILAFDRE LDVALLDRVV QTFYQGVGAE QQQAQQVLTQ FQAHPDAWSQ     50
AYSILEKSEY PQTKYIALSV LDKLITTRWK MLPKEQRLGI RNYIVAVMIK 100
NSSDETVLQQ QKTFLNKLDL TLVQILKQEW PHNWPNFIPE IVQASKTNLS 150
LCENNMIVLR LLSEEIFDYS AEQMTQLKTK NLKNQMCGEF AEIFQLCSQI 200
LERAQKPSLI KATLGTLLRF LNWIPLGYIF ETNIVELITN RFLNVPDFRN 250
VTIECLTEIA SLTSQPQYND KFVTMFNLVM TSVNSMLPLQ TDFREAYEES 300
STNEQDFIQN LALFLCAFFS SHLRPLENPE NQEVLLNAHS YLLNISRINE 350
REIFKICLEY WSKLVAQLYE EMQQIPMSEM NPLLNLSSPT SLISSNPNML 400
ANLPLRKHIY KDILSTLRLV MIENMVKPEE VLIVENDEGE IVREFVKETD 450
TITLYKSMRE VLVYLTHLDV VDTEIVMTEK LARIVVGTEW SWQNLNTLCW 500
AIGSISGAMN EEMEKRFLVN VIKDLLGLCE MKRGKDNKAV VASNIMYVVG 550
QYPRFLKAHW KFLKTVVNKL FEFMHEYHEG VQDMACDTFI KIAQKCRRHF 600
VAQQLGETEP FINEIIRNLA KTTEDLTPQQ THTFYEACGY MISAQPQKHL 650
QERLIFDLMA LPNQAWENIV AQAAQNAQVL GDPQTVKILA NVLKTNVAAC 700
TSIGSGFYPQ IAKNYVDMLG LYKAVSGLIS EVVAAQGNIA TKTPHVRGLR 750
TIKKEILKLV DAYISRAEDL ELVGNTLIPA LFEAVLLDYL QNVPDARDAE 800
VLNLITTIVN QLSELLTDKI PLVLDAVFGC TLEMISKDFS EYPEHRAAFF 850
QLLRAINLNC FPALLNIPAP QFKLVINSIV WSFKHVSRDI QETGLNILLE 900
LINNMASMGP DVSNAFFQTY YISLLQDILY VLTDSDHKSG FKLQSLILAR 950
LFYLVESNQI TVPLYDPSQF PQEMNNQLFL RQYIMNLLVT AFPHLQPIQI 1000
QEFVQTVLAL NQDSIKFKLA LRDFLIQLKE FGGDNAELYL EEKEQELAAQ 1050
QKAQLEKAMT VPGMIKPVDM PTMEEEEL 1078
Length:1,078
Mass (Da):123,567
Last modified:August 14, 2001 - v3
Checksum:i6CFA6955F1DF9F6D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 20817EIFQL…AQKPS → RFFNYAHKFSNVRKNLA no nucleotide entry 1 Publication
Add
BLAST
Sequence conflicti192 – 20817EIFQL…AQKPS → RFFNYAHKFSNVRKNLA in BAA03858. 1 Publication
Add
BLAST
Sequence conflicti484 – 4852IV → VI in AAG35722. 1 Publication
Sequence conflicti989 – 9891Missing no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15482 Genomic DNA. Translation: CAB40824.2.
D16355 Genomic DNA. Translation: BAA03858.1.
AB027496 Genomic DNA. Translation: BAA83345.1.
AB027497 Genomic DNA. Translation: BAA83346.1.
AF208056 Genomic DNA. Translation: AAG35722.1.
CU329670 Genomic DNA. Translation: CAB55858.1.
PIRiD45029.
T43511.
T50137.
RefSeqiXP_001713070.1. XM_001713018.2.

Genome annotation databases

EnsemblFungiiSPAC1805.17.1; SPAC1805.17.1:pep; SPAC1805.17.
GeneIDi3361487.
KEGGispo:SPAC1805.17.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15482 Genomic DNA. Translation: CAB40824.2 .
D16355 Genomic DNA. Translation: BAA03858.1 .
AB027496 Genomic DNA. Translation: BAA83345.1 .
AB027497 Genomic DNA. Translation: BAA83346.1 .
AF208056 Genomic DNA. Translation: AAG35722.1 .
CU329670 Genomic DNA. Translation: CAB55858.1 .
PIRi D45029.
T43511.
T50137.
RefSeqi XP_001713070.1. XM_001713018.2.

3D structure databases

ProteinModelPortali P14068.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 280563. 11 interactions.
MINTi MINT-4687292.
STRINGi 4896.SPAC1805.17-1.

Proteomic databases

MaxQBi P14068.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC1805.17.1 ; SPAC1805.17.1:pep ; SPAC1805.17 .
GeneIDi 3361487.
KEGGi spo:SPAC1805.17.

Organism-specific databases

PomBasei SPAC1805.17.

Phylogenomic databases

eggNOGi COG5101.
KOi K14290.
OMAi ILKQAWP.
OrthoDBi EOG7MWH56.
PhylomeDBi P14068.

Enzyme and pathway databases

Reactomei REACT_199398. Cyclin A/B1 associated events during G2/M transition.

Miscellaneous databases

NextBioi 20811530.
PROi P14068.

Family and domain databases

Gene3Di 1.25.10.10. 5 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR014877. CRM1_C_dom.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view ]
Pfami PF08767. CRM1_C. 1 hit.
PF03810. IBN_N. 1 hit.
PF08389. Xpo1. 1 hit.
[Graphical view ]
SMARTi SM01102. CRM1_C. 1 hit.
SM00913. IBN_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 4 hits.
PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery."
    Adachi Y., Yanagida M.
    J. Cell Biol. 108:1195-1207(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Adachi Y.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1."
    Toda T., Shimanuki M., Saka Y., Yamano H., Adachi Y., Shirakawa M., Kyogoku Y., Yanagida M.
    Mol. Cell. Biol. 12:5474-5484(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression."
    Nishi K., Yoshida M., Fujiwara D., Nishikawa M., Horinouchi S., Beppu T.
    J. Biol. Chem. 269:6320-6324(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-503 AND MET-546.
    Strain: LM102.
  5. "Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region."
    Kudo N., Matsumori N., Taoka H., Fujiwara D., Schreiner E.P., Wolff B., Yoshida M., Horinouchi S.
    Proc. Natl. Acad. Sci. U.S.A. 96:9112-9117(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-529.
    Strain: AC1 and FN41.
  6. "Sequence of Crm1/exportin 1 mutant alleles reveals critical sites associated with multidrug resistance."
    Carobbio S., Realini C., Norbury C.J., Toda T., Cavalli F., Spataro V.
    Curr. Genet. 39:2-9(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-129; GLU-299; GLU-430 AND PHE-992.
    Strain: 972 / HM123.
  7. "Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: their involvement in multidrug resistance, UV and pH sensitivity."
    Benko Z., Sipiczki M., Carr A.M.
    Mol. Gen. Genet. 260:434-443(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  8. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  9. "Both Php4 function and subcellular localization are regulated by iron via a multistep mechanism involving the glutaredoxin Grx4 and the exportin Crm1."
    Mercier A., Labbe S.
    J. Biol. Chem. 284:20249-20262(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PHP4.

Entry informationi

Entry nameiXPO1_SCHPO
AccessioniPrimary (citable) accession number: P14068
Secondary accession number(s): Q09197
, Q9HF66, Q9URF4, Q9UTF9, Q9UTG0, Q9UUL0, Q9UUL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: August 14, 2001
Last modified: September 3, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Cellular target of leptomycin B (LMB), a nuclear export inhibitor. LMB alkylates Cys-529 leading to CRM1 inactivation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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