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P14068

- XPO1_SCHPO

UniProt

P14068 - XPO1_SCHPO

Protein

Exportin-1

Gene

xpo1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Receptor for the leucine-rich nuclear export signal (NES).1 Publication

    GO - Molecular functioni

    1. GTP binding Source: PomBase
    2. nuclear export signal receptor activity Source: PomBase

    GO - Biological processi

    1. cellular response to oxidative stress Source: PomBase
    2. negative regulation of transcription from RNA polymerase II promoter Source: PomBase
    3. nuclear export Source: PomBase
    4. protein export from nucleus Source: PomBase
    5. protein targeting Source: PomBase

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199398. Cyclin A/B1 associated events during G2/M transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exportin-1
    Alternative name(s):
    Caffeine resistance protein 2
    Chromosome region maintenance protein 1
    Gene namesi
    Name:xpo1
    Synonyms:caf2, crm1
    ORF Names:SPAC1805.17, SPAC1B2.01
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC1805.17.

    Subcellular locationi

    Nucleus
    Note: Localized in the nucleus and at its periphery.

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nuclear envelope Source: PomBase
    3. nuclear periphery Source: PomBase
    4. nuclear pore Source: PomBase
    5. nucleus Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi129 – 1291E → K in crm1-1R-9R. 1 Publication
    Mutagenesisi299 – 2991E → K in crm1-14-R. 1 Publication
    Mutagenesisi430 – 4301E → K in crm1-809; cold-sensitive and LMB hypersensitive. 1 Publication
    Mutagenesisi503 – 5031G → D in crm1-N1. 1 Publication
    Mutagenesisi529 – 5291C → S in crm1-K1; LMB resistant. 1 Publication
    Mutagenesisi546 – 5461M → I in crm1-N1. 1 Publication
    Mutagenesisi992 – 9921F → S in crm1-119; LMB resistant. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10781078Exportin-1PRO_0000204709Add
    BLAST

    Proteomic databases

    MaxQBiP14068.

    Interactioni

    Subunit structurei

    Interacts with php4.1 Publication

    Protein-protein interaction databases

    BioGridi280563. 11 interactions.
    MINTiMINT-4687292.
    STRINGi4896.SPAC1805.17-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP14068.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 10067Importin N-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the exportin family.Curated
    Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5101.
    KOiK14290.
    OMAiILKQAWP.
    OrthoDBiEOG7MWH56.
    PhylomeDBiP14068.

    Family and domain databases

    Gene3Di1.25.10.10. 5 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR014877. CRM1_C_dom.
    IPR013598. Exportin-1/Importin-b-like.
    IPR001494. Importin-beta_N.
    [Graphical view]
    PfamiPF08767. CRM1_C. 1 hit.
    PF03810. IBN_N. 1 hit.
    PF08389. Xpo1. 1 hit.
    [Graphical view]
    SMARTiSM01102. CRM1_C. 1 hit.
    SM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 4 hits.
    PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14068-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGILAFDRE LDVALLDRVV QTFYQGVGAE QQQAQQVLTQ FQAHPDAWSQ     50
    AYSILEKSEY PQTKYIALSV LDKLITTRWK MLPKEQRLGI RNYIVAVMIK 100
    NSSDETVLQQ QKTFLNKLDL TLVQILKQEW PHNWPNFIPE IVQASKTNLS 150
    LCENNMIVLR LLSEEIFDYS AEQMTQLKTK NLKNQMCGEF AEIFQLCSQI 200
    LERAQKPSLI KATLGTLLRF LNWIPLGYIF ETNIVELITN RFLNVPDFRN 250
    VTIECLTEIA SLTSQPQYND KFVTMFNLVM TSVNSMLPLQ TDFREAYEES 300
    STNEQDFIQN LALFLCAFFS SHLRPLENPE NQEVLLNAHS YLLNISRINE 350
    REIFKICLEY WSKLVAQLYE EMQQIPMSEM NPLLNLSSPT SLISSNPNML 400
    ANLPLRKHIY KDILSTLRLV MIENMVKPEE VLIVENDEGE IVREFVKETD 450
    TITLYKSMRE VLVYLTHLDV VDTEIVMTEK LARIVVGTEW SWQNLNTLCW 500
    AIGSISGAMN EEMEKRFLVN VIKDLLGLCE MKRGKDNKAV VASNIMYVVG 550
    QYPRFLKAHW KFLKTVVNKL FEFMHEYHEG VQDMACDTFI KIAQKCRRHF 600
    VAQQLGETEP FINEIIRNLA KTTEDLTPQQ THTFYEACGY MISAQPQKHL 650
    QERLIFDLMA LPNQAWENIV AQAAQNAQVL GDPQTVKILA NVLKTNVAAC 700
    TSIGSGFYPQ IAKNYVDMLG LYKAVSGLIS EVVAAQGNIA TKTPHVRGLR 750
    TIKKEILKLV DAYISRAEDL ELVGNTLIPA LFEAVLLDYL QNVPDARDAE 800
    VLNLITTIVN QLSELLTDKI PLVLDAVFGC TLEMISKDFS EYPEHRAAFF 850
    QLLRAINLNC FPALLNIPAP QFKLVINSIV WSFKHVSRDI QETGLNILLE 900
    LINNMASMGP DVSNAFFQTY YISLLQDILY VLTDSDHKSG FKLQSLILAR 950
    LFYLVESNQI TVPLYDPSQF PQEMNNQLFL RQYIMNLLVT AFPHLQPIQI 1000
    QEFVQTVLAL NQDSIKFKLA LRDFLIQLKE FGGDNAELYL EEKEQELAAQ 1050
    QKAQLEKAMT VPGMIKPVDM PTMEEEEL 1078
    Length:1,078
    Mass (Da):123,567
    Last modified:August 14, 2001 - v3
    Checksum:i6CFA6955F1DF9F6D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti192 – 20817EIFQL…AQKPS → RFFNYAHKFSNVRKNLA no nucleotide entry (PubMed:1448080)CuratedAdd
    BLAST
    Sequence conflicti192 – 20817EIFQL…AQKPS → RFFNYAHKFSNVRKNLA in BAA03858. (PubMed:8119981)CuratedAdd
    BLAST
    Sequence conflicti484 – 4852IV → VI in AAG35722. (PubMed:11318103)Curated
    Sequence conflicti989 – 9891Missing no nucleotide entry (PubMed:1448080)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15482 Genomic DNA. Translation: CAB40824.2.
    D16355 Genomic DNA. Translation: BAA03858.1.
    AB027496 Genomic DNA. Translation: BAA83345.1.
    AB027497 Genomic DNA. Translation: BAA83346.1.
    AF208056 Genomic DNA. Translation: AAG35722.1.
    CU329670 Genomic DNA. Translation: CAB55858.1.
    PIRiD45029.
    T43511.
    T50137.
    RefSeqiXP_001713070.1. XM_001713018.2.

    Genome annotation databases

    EnsemblFungiiSPAC1805.17.1; SPAC1805.17.1:pep; SPAC1805.17.
    GeneIDi3361487.
    KEGGispo:SPAC1805.17.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15482 Genomic DNA. Translation: CAB40824.2 .
    D16355 Genomic DNA. Translation: BAA03858.1 .
    AB027496 Genomic DNA. Translation: BAA83345.1 .
    AB027497 Genomic DNA. Translation: BAA83346.1 .
    AF208056 Genomic DNA. Translation: AAG35722.1 .
    CU329670 Genomic DNA. Translation: CAB55858.1 .
    PIRi D45029.
    T43511.
    T50137.
    RefSeqi XP_001713070.1. XM_001713018.2.

    3D structure databases

    ProteinModelPortali P14068.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 280563. 11 interactions.
    MINTi MINT-4687292.
    STRINGi 4896.SPAC1805.17-1.

    Proteomic databases

    MaxQBi P14068.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC1805.17.1 ; SPAC1805.17.1:pep ; SPAC1805.17 .
    GeneIDi 3361487.
    KEGGi spo:SPAC1805.17.

    Organism-specific databases

    PomBasei SPAC1805.17.

    Phylogenomic databases

    eggNOGi COG5101.
    KOi K14290.
    OMAi ILKQAWP.
    OrthoDBi EOG7MWH56.
    PhylomeDBi P14068.

    Enzyme and pathway databases

    Reactomei REACT_199398. Cyclin A/B1 associated events during G2/M transition.

    Miscellaneous databases

    NextBioi 20811530.
    PROi P14068.

    Family and domain databases

    Gene3Di 1.25.10.10. 5 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR014877. CRM1_C_dom.
    IPR013598. Exportin-1/Importin-b-like.
    IPR001494. Importin-beta_N.
    [Graphical view ]
    Pfami PF08767. CRM1_C. 1 hit.
    PF03810. IBN_N. 1 hit.
    PF08389. Xpo1. 1 hit.
    [Graphical view ]
    SMARTi SM01102. CRM1_C. 1 hit.
    SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 4 hits.
    PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery."
      Adachi Y., Yanagida M.
      J. Cell Biol. 108:1195-1207(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Adachi Y.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1."
      Toda T., Shimanuki M., Saka Y., Yamano H., Adachi Y., Shirakawa M., Kyogoku Y., Yanagida M.
      Mol. Cell. Biol. 12:5474-5484(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression."
      Nishi K., Yoshida M., Fujiwara D., Nishikawa M., Horinouchi S., Beppu T.
      J. Biol. Chem. 269:6320-6324(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-503 AND MET-546.
      Strain: LM102.
    5. "Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region."
      Kudo N., Matsumori N., Taoka H., Fujiwara D., Schreiner E.P., Wolff B., Yoshida M., Horinouchi S.
      Proc. Natl. Acad. Sci. U.S.A. 96:9112-9117(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-529.
      Strain: AC1 and FN41.
    6. "Sequence of Crm1/exportin 1 mutant alleles reveals critical sites associated with multidrug resistance."
      Carobbio S., Realini C., Norbury C.J., Toda T., Cavalli F., Spataro V.
      Curr. Genet. 39:2-9(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-129; GLU-299; GLU-430 AND PHE-992.
      Strain: 972 / HM123.
    7. "Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: their involvement in multidrug resistance, UV and pH sensitivity."
      Benko Z., Sipiczki M., Carr A.M.
      Mol. Gen. Genet. 260:434-443(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    8. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    9. "Both Php4 function and subcellular localization are regulated by iron via a multistep mechanism involving the glutaredoxin Grx4 and the exportin Crm1."
      Mercier A., Labbe S.
      J. Biol. Chem. 284:20249-20262(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PHP4.

    Entry informationi

    Entry nameiXPO1_SCHPO
    AccessioniPrimary (citable) accession number: P14068
    Secondary accession number(s): Q09197
    , Q9HF66, Q9URF4, Q9UTF9, Q9UTG0, Q9UUL0, Q9UUL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Cellular target of leptomycin B (LMB), a nuclear export inhibitor. LMB alkylates Cys-529 leading to CRM1 inactivation.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3