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Protein

Glycerol 2-dehydrogenase (NADP(+))

Gene

GCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycerol dehydrogenase involved in glycerol catabolism under microaerobic conditions. Has mRNA binding activity.6 Publications

Catalytic activityi

Glycerol + NADP+ = glycerone + NADPH.4 Publications

Kineticsi

  1. KM=11.3 mM for D,L-glyceraldehyde4 Publications
  2. KM=0.007 mM for NADPH4 Publications
  3. KM=0.13 mM for p-nitrobenzaldehyde4 Publications
  4. KM=5.2 mM for benzaldehyde4 Publications
  5. KM=8.7 mM for phenylglyoxal4 Publications
  6. KM=50 mM for acrolein4 Publications
  7. KM=54 mM for butyraldehyde4 Publications
  8. KM=0.724 mM for ethyl-4-chloro-3-oxo-butanoate4 Publications

    pH dependencei

    Optimum pH is 6.5.4 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei56Proton donorBy similarity1
    Sitei81Lowers pKa of active site TyrBy similarity1
    Binding sitei112SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi220 – 274NADPBy similarityAdd BLAST55

    GO - Molecular functioni

    • alditol:NADP+ 1-oxidoreductase activity Source: SGD
    • aldo-keto reductase (NADP) activity Source: SGD
    • glycerol 2-dehydrogenase (NADP+) activity Source: UniProtKB-EC
    • glycerol dehydrogenase [NAD(P)+] activity Source: SGD
    • mRNA binding Source: SGD
    • oxidoreductase activity Source: SGD

    GO - Biological processi

    • arabinose catabolic process Source: SGD
    • cellular response to oxidative stress Source: SGD
    • D-xylose catabolic process Source: SGD
    • glycerol metabolic process Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycerol metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:YOR120W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol 2-dehydrogenase (NADP(+)) (EC:1.1.1.156)
    Alternative name(s):
    Galactose-inducible crystallin-like protein 1
    Gene namesi
    Name:GCY1
    Synonyms:GCY
    Ordered Locus Names:YOR120W
    ORF Names:O31567, YOR3269W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR120W.
    SGDiS000005646. GCY1.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi29Q → K: Decreases catalytic activity. 1 Publication1
    Mutagenesisi56Y → L: Loss of catalytic activity. 1 Publication1
    Mutagenesisi264K → R: Decreases catalytic activity. 1 Publication1
    Mutagenesisi267N → Q: Decreases catalytic activity. 1 Publication1
    Mutagenesisi270R → H, Y or K: Decreases catalytic activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001246111 – 312Glycerol 2-dehydrogenase (NADP(+))Add BLAST312

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei306PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP14065.
    PRIDEiP14065.
    TopDownProteomicsiP14065.

    PTM databases

    iPTMnetiP14065.

    Expressioni

    Inductioni

    Expression is under the control of GAL4 and REB1, and is both positively controlled by galactose and negatively by glucose. Also induced by salt stress and in response to DNA replication stress.5 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi34516. 27 interactors.
    DIPiDIP-6342N.
    IntActiP14065. 1 interactor.
    MINTiMINT-2493441.

    Structurei

    3D structure databases

    ProteinModelPortaliP14065.
    SMRiP14065.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00850000132445.
    HOGENOMiHOG000250272.
    InParanoidiP14065.
    KOiK18097.
    OMAiNEELNDY.
    OrthoDBiEOG092C324N.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 2 hits.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14065-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPATLHDSTK ILSLNTGAQI PQIGLGTWQS KENDAYKAVL TALKDGYRHI
    60 70 80 90 100
    DTAAIYRNED QVGQAIKDSG VPREEIFVTT KLWCTQHHEP EVALDQSLKR
    110 120 130 140 150
    LGLDYVDLYL MHWPARLDPA YIKNEDILSV PTKKDGSRAV DITNWNFIKT
    160 170 180 190 200
    WELMQELPKT GKTKAVGVSN FSINNLKDLL ASQGNKLTPA ANQVEIHPLL
    210 220 230 240 250
    PQDELINFCK SKGIVVEAYS PLGSTDAPLL KEPVILEIAK KNNVQPGHVV
    260 270 280 290 300
    ISWHVQRGYV VLPKSVNPDR IKTNRKIFTL STEDFEAINN ISKEKGEKRV
    310
    VHPNWSPFEV FK
    Length:312
    Mass (Da):35,079
    Last modified:January 1, 1990 - v1
    Checksum:i77B9D31C228334A4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X13228 Genomic DNA. Translation: CAA31615.1.
    X96740 Genomic DNA. Translation: CAA65512.1.
    X90518 Genomic DNA. Translation: CAA62107.1.
    X94335 Genomic DNA. Translation: CAA64040.1.
    Z75028 Genomic DNA. Translation: CAA99318.1.
    BK006948 Genomic DNA. Translation: DAA10895.1.
    PIRiS22846.
    RefSeqiNP_014763.1. NM_001183539.1.

    Genome annotation databases

    EnsemblFungiiYOR120W; YOR120W; YOR120W.
    GeneIDi854287.
    KEGGisce:YOR120W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X13228 Genomic DNA. Translation: CAA31615.1.
    X96740 Genomic DNA. Translation: CAA65512.1.
    X90518 Genomic DNA. Translation: CAA62107.1.
    X94335 Genomic DNA. Translation: CAA64040.1.
    Z75028 Genomic DNA. Translation: CAA99318.1.
    BK006948 Genomic DNA. Translation: DAA10895.1.
    PIRiS22846.
    RefSeqiNP_014763.1. NM_001183539.1.

    3D structure databases

    ProteinModelPortaliP14065.
    SMRiP14065.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi34516. 27 interactors.
    DIPiDIP-6342N.
    IntActiP14065. 1 interactor.
    MINTiMINT-2493441.

    PTM databases

    iPTMnetiP14065.

    Proteomic databases

    MaxQBiP14065.
    PRIDEiP14065.
    TopDownProteomicsiP14065.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYOR120W; YOR120W; YOR120W.
    GeneIDi854287.
    KEGGisce:YOR120W.

    Organism-specific databases

    EuPathDBiFungiDB:YOR120W.
    SGDiS000005646. GCY1.

    Phylogenomic databases

    GeneTreeiENSGT00850000132445.
    HOGENOMiHOG000250272.
    InParanoidiP14065.
    KOiK18097.
    OMAiNEELNDY.
    OrthoDBiEOG092C324N.

    Enzyme and pathway databases

    BioCyciYEAST:YOR120W-MONOMER.

    Miscellaneous databases

    PROiP14065.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 2 hits.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGCY1_YEAST
    AccessioniPrimary (citable) accession number: P14065
    Secondary accession number(s): D6W2H9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: November 30, 2016
    This is version 154 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.