Reviewed, UniProtKB/Swiss-Prot P14065 (GCY_YEAST)

Names and origin

Protein names

Recommended name:
    Protein GCY
    EC=1.1.1.-

Gene names

Name:	GCY1
Synonyms:	GCY
Ordered Locus Names:	YOR120W
ORF Names:	O31567, YOR3269W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Unknown function which seems to be not essential.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose catabolic process Inferred from direct assay. Source: SGD arabinose catabolic process Inferred from direct assay. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from genetic interaction. Source: SGD response to salt stress Inferred from expression pattern. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	aldehyde reductase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
INO1	P11986	1	EBI-7505,EBI-9257
RNR2	P09938	1	EBI-7505,EBI-15240

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Protein GCY		PRO_0000124611
Regions
	Nucleotide binding	220 – 274	55	NADP By similarity
Sites
	Active site	56	1	Proton donor By similarity
	Binding site	112	1	Substrate By similarity
	Site	81	1	Lowers pKa of active site Tyr By similarity
Amino acid modifications
	Modified residue	4	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P14065-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 77B9D31C228334A4	FASTA	312	35,079
	10 20 30 40 50 60 MPATLHDSTK ILSLNTGAQI PQIGLGTWQS KENDAYKAVL TALKDGYRHI DTAAIYRNED 70 80 90 100 110 120 QVGQAIKDSG VPREEIFVTT KLWCTQHHEP EVALDQSLKR LGLDYVDLYL MHWPARLDPA 130 140 150 160 170 180 YIKNEDILSV PTKKDGSRAV DITNWNFIKT WELMQELPKT GKTKAVGVSN FSINNLKDLL 190 200 210 220 230 240 ASQGNKLTPA ANQVEIHPLL PQDELINFCK SKGIVVEAYS PLGSTDAPLL KEPVILEIAK 250 260 270 280 290 300 KNNVQPGHVV ISWHVQRGYV VLPKSVNPDR IKTNRKIFTL STEDFEAINN ISKEKGEKRV 310 VHPNWSPFEV FK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A nuclear yeast gene (GCY) encodes a polypeptide with high homology to a vertebrate eye lens protein." Oechsner U., Magdolen V., Bandlow W. FEBS Lett. 238:123-128(1988) [PubMed: 2901985] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"The type of basal promoter determines the regulated or constitutive mode of transcription in the common control region of the yeast gene pair GCY1/RIO1." Angermayr M., Bandlow W. J. Biol. Chem. 272:31630-31635(1997) [PubMed: 9395503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25657.
[3]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed: 8904341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-4, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13228 Genomic DNA. Translation: CAA31615.1. X96740 Genomic DNA. Translation: CAA65512.1. X90518 Genomic DNA. Translation: CAA62107.1. X94335 Genomic DNA. Translation: CAA64040.1. Z75028 Genomic DNA. Translation: CAA99318.1.
PIR	S22846.
RefSeq	NP_014763.1.
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6342N.
IntAct	P14065. 5 interactions.
Proteomic databases
PeptideAtlas	P14065.
Genome annotation databases
Ensembl	YOR120W. Saccharomyces cerevisiae. [Contig view]
GeneID	854287.
GenomeReviews	Gene locus YOR120W in contig Y13140_GR.
KEGG	sce:YOR120W.
NMPDR	fig|4932.3.peg.5866.
Organism-specific databases
CYGD	YOR120w.
SGD	S000005646. GCY1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P14065.
OMA	P14065. ANILISW.
Gene expression databases
ArrayExpress	P14065.
GermOnline	YOR120W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976269.

Entry information

Entry name

GCY_YEAST

Accession

Primary (citable) accession number: P14065

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)