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Protein

Transcriptional activator HAP4

Gene

HAP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP4 encodes a regulatory subunit of the DNA-bound complex and seems to provide the principal transcriptional activation domains. Does not bind DNA directly, but augments the binding of HAP2 and HAP3.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • RNA polymerase II activating transcription factor binding Source: SGD
  • transcription factor activity, RNA polymerase II transcription factor binding Source: SGD

GO - Biological processi

  • carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of cellular respiration Source: SGD
  • transcription by RNA polymerase II Source: GOC

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31895-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator HAP4
Gene namesi
Name:HAP4
Ordered Locus Names:YKL109W
ORF Names:YKL465
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL109W
SGDiS000001592 HAP4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148 – 151FLKF → SSKS: Greatly diminishes transcriptional activation. 1 Publication4
Mutagenesisi390 – 394IWNYL → SSNSS: Greatly diminishes transcriptional activation. 1 Publication5
Mutagenesisi427 – 430YLFL → SSSS: Greatly diminishes transcriptional activation. 1 Publication4
Mutagenesisi456 – 459FSYL → SSSS: Greatly diminishes transcriptional activation. 1 Publication4
Mutagenesisi466 – 467LM → SS: Abolishes transcriptional activation. 2
Mutagenesisi466L → A: Greatly diminishes transcriptional activation. 1 Publication1
Mutagenesisi467M → A: Abolishes transcriptional activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839001 – 554Transcriptional activator HAP4Add BLAST554

Proteomic databases

PaxDbiP14064
PRIDEiP14064

PTM databases

iPTMnetiP14064

Expressioni

Inductioni

Its expression is glucose-repressible.

Interactioni

Subunit structurei

Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.1 Publication

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi34024, 283 interactors
DIPiDIP-2360N
IntActiP14064, 3 interactors
MINTiP14064
STRINGi4932.YKL109W

Structurei

3D structure databases

ProteinModelPortaliP14064
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 327Probably encodes all the information necessary to anchor HAP4 to HAP2/3Add BLAST327
Regioni124 – 300Activation domain 1Add BLAST177
Regioni359 – 476Activation domain 2Add BLAST118

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi54 – 80Arg/Lys-rich (basic)Add BLAST27
Compositional biasi90 – 113Asn-richAdd BLAST24
Compositional biasi107 – 113Poly-Asn7
Compositional biasi424 – 471Asp/Glu-rich (acidic)Add BLAST48
Compositional biasi519 – 549Asp/Glu-rich (acidic)Add BLAST31

Phylogenomic databases

InParanoidiP14064
OrthoDBiEOG092C22W5

Family and domain databases

InterProiView protein in InterPro
IPR018287 Hap4_TF_heteromerisation
PfamiView protein in Pfam
PF10297 Hap4_Hap_bind, 1 hit

Sequencei

Sequence statusi: Complete.

P14064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKTFLLQA SASRPRSNHF KNEHNNIPLA PVPIAPNTNH HNNSSLEFEN
60 70 80 90 100
DGSKKKKKSS LVVRTSKHWV LPPRPRPGRR SSSHNTLPAN NTNNILNVGP
110 120 130 140 150
NSRNSSNNNN NNNIISNRKQ ASKEKRKIPR HIQTIDEKLI NDSNYLAFLK
160 170 180 190 200
FDDLENEKFH SSASSISSPS YSSPSFSSYR NRKKSEFMDD ESCTDVETIA
210 220 230 240 250
AHNSLLTKNH HIDSSSNVHA PPTKKSKLND FDLLSLSSTS SSATPVPQLT
260 270 280 290 300
KDLNMNLNFH KIPHKASFPD SPADFSPADS VSLIRNHSLP TNLQVKDKIE
310 320 330 340 350
DLNEIKFFND FEKLEFFNKY AKVNTNNDVN ENNDLWNSYL QSMDDTTGKN
360 370 380 390 400
SGNYQQVDND DNMSLLNLPI LEETVSSGQD DKVEPDEEDI WNYLPSSSSQ
410 420 430 440 450
QEDSSRALKK NTNSEKANIQ AKNDETYLFL QDQDESADSH HHDELGSEIT
460 470 480 490 500
LADNKFSYLP PTLEELMEEQ DCNNGRSFKN FMFSNDTGID GSAGTDDDYT
510 520 530 540 550
KVLKSKKIST SKSNANLYDL NDNNNDATAT NELDQSSFID DLDEDVDFLK

VQVF
Length:554
Mass (Da):62,412
Last modified:June 1, 1994 - v2
Checksum:iCF8A46219F8CA431
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160H → R in CAA34707 (PubMed:2676721).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16727 Genomic DNA Translation: CAA34707.1
X71133 Genomic DNA Translation: CAA50448.1
Z28109 Genomic DNA Translation: CAA81949.1
BK006944 Genomic DNA Translation: DAA09048.1
PIRiS37936
RefSeqiNP_012813.1, NM_001179675.1

Genome annotation databases

EnsemblFungiiYKL109W; YKL109W; YKL109W
GeneIDi853751
KEGGisce:YKL109W

Similar proteinsi

Entry informationi

Entry nameiHAP4_YEAST
AccessioniPrimary (citable) accession number: P14064
Secondary accession number(s): D6VXH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health