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P14056 (ARAF_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase A-Raf
EC=2.7.11.1
Alternative name(s):
Proto-oncogene A-Raf
Proto-oncogene A-Raf-1
Gene names
Name:Araf
Synonyms:A-raf, Araf1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Interacts with TH1L/NELFD By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 protein kinase domain.

Contains 1 RBD (Ras-binding) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 604604Serine/threonine-protein kinase A-Raf
PRO_0000085625

Regions

Domain19 – 9173RBD
Domain308 – 568261Protein kinase
Zinc finger98 – 14447Phorbol-ester/DAG-type
Nucleotide binding314 – 3229ATP By similarity

Sites

Active site4271Proton acceptor By similarity
Metal binding991Zinc 1 By similarity
Metal binding1121Zinc 2 By similarity
Metal binding1151Zinc 2 By similarity
Metal binding1251Zinc 1 By similarity
Metal binding1281Zinc 1 By similarity
Metal binding1331Zinc 2 By similarity
Metal binding1361Zinc 2 By similarity
Metal binding1441Zinc 1 By similarity
Binding site3341ATP By similarity

Amino acid modifications

Modified residue1861Phosphoserine By similarity
Modified residue2131Phosphothreonine By similarity
Modified residue2151Phosphothreonine By similarity
Modified residue2551Phosphoserine By similarity
Modified residue3161Phosphothreonine By similarity
Modified residue5781Phosphoserine By similarity
Modified residue5801Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P14056 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: FF24FB2170B0B115

FASTA60467,551
        10         20         30         40         50         60 
MEPPRGPPAS GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV 

        70         80         90        100        110        120 
VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF 

       130        140        150        160        170        180 
HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RRQFYHSIQD LSGGSRQQEV PSNLSVNELL 

       190        200        210        220        230        240 
TPQGPSPFTQ QRDQEHFSFP APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS 

       250        260        270        280        290        300 
TDAAGRGGDG APRGSPSPAS VSSGRKSPHS KLPAEQRERK SLADEKKKVK NLGYRDSGYY 

       310        320        330        340        350        360 
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK NEMQVLRKTR 

       370        380        390        400        410        420 
HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD MVQLIDVARQ TAQGMDYLHA 

       430        440        450        460        470        480 
KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD 

       490        500        510        520        530        540 
PNPYSFQSDV YAYGVVLYEL MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM 

       550        560        570        580        590        600 
RRLLTDCLKF QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA 


RLVP

« Hide

References

[1]"The complete primary structure of the rat A-raf cDNA coding region: conservation of the putative regulatory regions present in rat c-raf."
Ishikawa F., Takaku F., Nagao M., Sugimura T.
Oncogene Res. 1:243-253(1987) [PubMed: 3449797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Fischer.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X06942 mRNA. Translation: CAA30023.1.
IPIIPI00563950.
PIRS00726.
RefSeqNP_071977.1. NM_022532.2.
UniGeneRn.1714.

3D structure databases

ProteinModelPortalP14056.
SMRP14056. Positions 20-91, 96-146, 301-574.
ModBaseSearch...

Protein-protein interaction databases

STRINGP14056.

PTM databases

PhosphoSiteP14056.

Proteomic databases

PRIDEP14056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000039911; ENSRNOP00000048890; ENSRNOG00000010838.
GeneID64363.
KEGGrno:64363.
UCSCNM_022532. rat.

Organism-specific databases

CTD369.
RGD2148. Araf.

Phylogenomic databases

eggNOGroNOG10943.
HOVERGENHBG001886.
OrthoDBEOG4CRKZT.

Enzyme and pathway databases

BRENDA2.7.10.2. 5301.

Gene expression databases

ArrayExpressP14056.
GenevestigatorP14056.
GermOnlineENSRNOG00000010838. Rattus norvegicus.

Family and domain databases

InterProIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras-bd.
IPR001245. Ser-Thr/Tyr_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
KOK08286.
PfamPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSPR00008. DAGPEDOMAIN.
SMARTSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613074.

Entry information

Entry nameARAF_RAT
AccessionPrimary (citable) accession number: P14056
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 16, 2011
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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