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Protein

Serine/threonine-protein kinase A-Raf

Gene

Araf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Zinc 1By similarity1
Metal bindingi112Zinc 2By similarity1
Metal bindingi115Zinc 2By similarity1
Metal bindingi125Zinc 1By similarity1
Metal bindingi128Zinc 1By similarity1
Metal bindingi133Zinc 2By similarity1
Metal bindingi136Zinc 2By similarity1
Metal bindingi144Zinc 1By similarity1
Binding sitei334ATPPROSITE-ProRule annotation1
Active sitei427Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi314 – 322ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2 5301
ReactomeiR-RNO-5673000 RAF activation
R-RNO-5674135 MAP2K and MAPK activation
R-RNO-5675221 Negative regulation of MAPK pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene A-Raf
Proto-oncogene A-Raf-1
Gene namesi
Name:Araf
Synonyms:A-raf, Araf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2148 Araf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856251 – 604Serine/threonine-protein kinase A-RafAdd BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei181PhosphothreonineCombined sources1
Modified residuei186PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei316PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP14056
PRIDEiP14056

PTM databases

iPTMnetiP14056
PhosphoSitePlusiP14056

Expressioni

Gene expression databases

BgeeiENSRNOG00000010838
GenevisibleiP14056 RN

Interactioni

Subunit structurei

Interacts with TH1L/NELFD.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249047, 3 interactors
STRINGi10116.ENSRNOP00000048890

Structurei

3D structure databases

ProteinModelPortaliP14056
SMRiP14056
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 91RBDPROSITE-ProRule annotationAdd BLAST73
Domaini308 – 568Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00900000140880
HOGENOMiHOG000252972
HOVERGENiHBG001886
InParanoidiP14056
KOiK08845
OMAiQHRDPEH
OrthoDBiEOG091G09SB
PhylomeDBiP14056
TreeFamiTF317006

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR003116 RBD_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF02196 RBD, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00455 RBD, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50898 RBD, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P14056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPRGPPAS GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK
60 70 80 90 100
VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN
110 120 130 140 150
FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN
160 170 180 190 200
RRQFYHSIQD LSGGSRQQEV PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP
210 220 230 240 250
APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG
260 270 280 290 300
APRGSPSPAS VSSGRKSPHS KLPAEQRERK SLADEKKKVK NLGYRDSGYY
310 320 330 340 350
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK
360 370 380 390 400
NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD
410 420 430 440 450
MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT
460 470 480 490 500
VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL
510 520 530 540 550
MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF
560 570 580 590 600
QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA

RLVP
Length:604
Mass (Da):67,551
Last modified:January 1, 1990 - v1
Checksum:iFF24FB2170B0B115
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06942 mRNA Translation: CAA30023.1
PIRiS00726
RefSeqiNP_071977.1, NM_022532.2
UniGeneiRn.1714

Genome annotation databases

EnsembliENSRNOT00000039911; ENSRNOP00000048890; ENSRNOG00000010838
GeneIDi64363
KEGGirno:64363
UCSCiRGD:2148 rat

Similar proteinsi

Entry informationi

Entry nameiARAF_RAT
AccessioniPrimary (citable) accession number: P14056
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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