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Protein

Serine/threonine-protein kinase A-Raf

Gene

Araf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Zinc 1By similarity
Metal bindingi112 – 1121Zinc 2By similarity
Metal bindingi115 – 1151Zinc 2By similarity
Metal bindingi125 – 1251Zinc 1By similarity
Metal bindingi128 – 1281Zinc 1By similarity
Metal bindingi133 – 1331Zinc 2By similarity
Metal bindingi136 – 1361Zinc 2By similarity
Metal bindingi144 – 1441Zinc 1By similarity
Binding sitei334 – 3341ATPPROSITE-ProRule annotation
Active sitei427 – 4271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 14447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi314 – 3229ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase kinase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2. 5301.
ReactomeiR-RNO-187706. Signalling to p38 via RIT and RIN.
R-RNO-5673000. RAF activation.
R-RNO-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene A-Raf
Proto-oncogene A-Raf-1
Gene namesi
Name:Araf
Synonyms:A-raf, Araf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2148. Araf.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Serine/threonine-protein kinase A-RafPRO_0000085625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571PhosphoserineBy similarity
Modified residuei162 – 1621PhosphoserineBy similarity
Modified residuei181 – 1811PhosphothreonineCombined sources
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei255 – 2551PhosphoserineBy similarity
Modified residuei267 – 2671PhosphoserineBy similarity
Modified residuei316 – 3161PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP14056.

PTM databases

iPTMnetiP14056.
PhosphoSiteiP14056.

Expressioni

Gene expression databases

ExpressionAtlasiP14056. baseline and differential.
GenevisibleiP14056. RN.

Interactioni

Subunit structurei

Interacts with TH1L/NELFD.By similarity

Protein-protein interaction databases

BioGridi249047. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP14056.
SMRiP14056. Positions 20-91, 96-146, 301-574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9173RBDPROSITE-ProRule annotationAdd
BLAST
Domaini308 – 568261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 14447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP14056.
KOiK08845.
OMAiLMQLTAQ.
OrthoDBiEOG091G09SB.
PhylomeDBiP14056.
TreeFamiTF317006.

Family and domain databases

InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPRGPPAS GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK
60 70 80 90 100
VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN
110 120 130 140 150
FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN
160 170 180 190 200
RRQFYHSIQD LSGGSRQQEV PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP
210 220 230 240 250
APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG
260 270 280 290 300
APRGSPSPAS VSSGRKSPHS KLPAEQRERK SLADEKKKVK NLGYRDSGYY
310 320 330 340 350
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK
360 370 380 390 400
NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD
410 420 430 440 450
MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT
460 470 480 490 500
VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL
510 520 530 540 550
MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF
560 570 580 590 600
QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA

RLVP
Length:604
Mass (Da):67,551
Last modified:January 1, 1990 - v1
Checksum:iFF24FB2170B0B115
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06942 mRNA. Translation: CAA30023.1.
PIRiS00726.
RefSeqiNP_071977.1. NM_022532.2.
UniGeneiRn.1714.

Genome annotation databases

EnsembliENSRNOT00000039911; ENSRNOP00000048890; ENSRNOG00000010838.
GeneIDi64363.
KEGGirno:64363.
UCSCiRGD:2148. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06942 mRNA. Translation: CAA30023.1.
PIRiS00726.
RefSeqiNP_071977.1. NM_022532.2.
UniGeneiRn.1714.

3D structure databases

ProteinModelPortaliP14056.
SMRiP14056. Positions 20-91, 96-146, 301-574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249047. 3 interactions.

PTM databases

iPTMnetiP14056.
PhosphoSiteiP14056.

Proteomic databases

PRIDEiP14056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000039911; ENSRNOP00000048890; ENSRNOG00000010838.
GeneIDi64363.
KEGGirno:64363.
UCSCiRGD:2148. rat.

Organism-specific databases

CTDi369.
RGDi2148. Araf.

Phylogenomic databases

GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP14056.
KOiK08845.
OMAiLMQLTAQ.
OrthoDBiEOG091G09SB.
PhylomeDBiP14056.
TreeFamiTF317006.

Enzyme and pathway databases

BRENDAi2.7.10.2. 5301.
ReactomeiR-RNO-187706. Signalling to p38 via RIT and RIN.
R-RNO-5673000. RAF activation.
R-RNO-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

PROiP14056.

Gene expression databases

ExpressionAtlasiP14056. baseline and differential.
GenevisibleiP14056. RN.

Family and domain databases

InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARAF_RAT
AccessioniPrimary (citable) accession number: P14056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 7, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.