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Protein

Dolichol-phosphate mannosyltransferase

Gene

DPM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.

Miscellaneous

Present with 1885 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate.

Kineticsi

For the phosphorylated protein, the Vmax increases to 146.7 nmol/min/mg enzyme, whereas the KM stays at 1.1 µM for GDP-mannose, increasing the catalytic efficiency 6-fold.
  1. KM=1.2 µM for GDP-mannose1 Publication
  1. Vmax=25.1 nmol/min/mg enzyme for GDP-mannose1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate beta-D-mannosyltransferase activity Source: SGD

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: SGD
  • GPI anchor biosynthetic process Source: SGD

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YPR183W-MONOMER
YEAST:YPR183W-MONOMER
BRENDAi2.4.1.83 984
SABIO-RKiP14020
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT2 Glycosyltransferase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichol-phosphate mannosyltransferase (EC:2.4.1.83)
Alternative name(s):
Dolichol-phosphate mannose synthase
Short name:
DPM synthase
Dolichyl-phosphate beta-D-mannosyltransferase
Mannose-P-dolichol synthase
Short name:
MPD synthase
Gene namesi
Name:DPM1
Synonyms:SED3
Ordered Locus Names:YPR183W
ORF Names:P9705.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR183W
SGDiS000006387 DPM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 238CytoplasmicSequence analysisAdd BLAST237
Transmembranei239 – 259Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini260 – 267LumenalSequence analysis8

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi141S → A: Reduces specific activity 2-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000591752 – 267Dolichol-phosphate mannosyltransferaseAdd BLAST266

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei141Phosphoserine; by PKA1 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP14020
PaxDbiP14020
PRIDEiP14020

PTM databases

iPTMnetiP14020

Interactioni

Subunit structurei

Interacts with the C-terminus of SAC1, thereby sequestering it to the endoplasmic reticulum in exponentially growing cells. Under nutrient limitation conditions, this interaction is rapidly abolished.1 Publication

Protein-protein interaction databases

BioGridi36355463 interactors.
DIPiDIP-4087N
IntActiP14020 14 interactors.
STRINGi4932.YPR183W

Structurei

3D structure databases

ProteinModelPortaliP14020
SMRiP14020
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075000
HOGENOMiHOG000283250
InParanoidiP14020
KOiK00721
OMAiFQVEMNY
OrthoDBiEOG092C4DAK

Family and domain databases

Gene3Di3.90.550.101 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEYSVIVP AYHEKLNIKP LTTRLFAGMS PEMAKKTELI FVDDNSQDGS
60 70 80 90 100
VEEVDALAHQ GYNVRIIVRT NERGLSSAVL KGFYEAKGQY LVCMDADLQH
110 120 130 140 150
PPETVPKLFE SLHDHAFTLG TRYAPGVGID KDWPMYRRVI SSTARMMARP
160 170 180 190 200
LTIASDPMSG FFGLQKKYLE NCNPRDINSQ GFKIALELLA KLPLPRDPRV
210 220 230 240 250
AIGEVPFTFG VRTEGESKLS GKVIIQYLQQ LKELYVFKFG ANNLILFITF
260
WSILFFYVCY QLYHLVF
Length:267
Mass (Da):30,362
Last modified:January 23, 2007 - v3
Checksum:i14F2F59F7B6A391A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04184 Genomic DNA Translation: AAA34578.1
U25842 Genomic DNA Translation: AAB68116.1
BK006949 Genomic DNA Translation: DAA11599.1
PIRiA32122
RefSeqiNP_015509.1, NM_001184280.1

Genome annotation databases

EnsemblFungiiYPR183W; YPR183W; YPR183W
GeneIDi856313
KEGGisce:YPR183W

Similar proteinsi

Entry informationi

Entry nameiDPM1_YEAST
AccessioniPrimary (citable) accession number: P14020
Secondary accession number(s): D6W4I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome