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Protein

Dolichol-phosphate mannosyltransferase

Gene

DPM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.

Catalytic activityi

GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate.

Kineticsi

For the phosphorylated protein, the Vmax increases to 146.7 nmol/min/mg enzyme, whereas the KM stays at 1.1 µM for GDP-mannose, increasing the catalytic efficiency 6-fold.

  1. KM=1.2 µM for GDP-mannose1 Publication
  1. Vmax=25.1 nmol/min/mg enzyme for GDP-mannose1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate beta-D-mannosyltransferase activity Source: SGD

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: SGD
  • GPI anchor biosynthetic process Source: SGD
  • mannosylation Source: GOC
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7201.
YEAST:YPR183W-MONOMER.
BRENDAi2.4.1.83. 984.
SABIO-RKP14020.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichol-phosphate mannosyltransferase (EC:2.4.1.83)
Alternative name(s):
Dolichol-phosphate mannose synthase
Short name:
DPM synthase
Dolichyl-phosphate beta-D-mannosyltransferase
Mannose-P-dolichol synthase
Short name:
MPD synthase
Gene namesi
Name:DPM1
Synonyms:SED3
Ordered Locus Names:YPR183W
ORF Names:P9705.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR183W.
SGDiS000006387. DPM1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 238237CytoplasmicSequence analysisAdd
BLAST
Transmembranei239 – 25921Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini260 – 2678LumenalSequence analysis

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi141 – 1411S → A: Reduces specific activity 2-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 267266Dolichol-phosphate mannosyltransferasePRO_0000059175Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei141 – 1411Phosphoserine; by PKA1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP14020.
PeptideAtlasiP14020.

PTM databases

iPTMnetiP14020.

Interactioni

Subunit structurei

Interacts with the C-terminus of SAC1, thereby sequestering it to the endoplasmic reticulum in exponentially growing cells. Under nutrient limitation conditions, this interaction is rapidly abolished.1 Publication

Protein-protein interaction databases

BioGridi36355. 77 interactions.
DIPiDIP-4087N.
IntActiP14020. 11 interactions.
MINTiMINT-486015.

Structurei

3D structure databases

ProteinModelPortaliP14020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075000.
HOGENOMiHOG000283250.
InParanoidiP14020.
KOiK00721.
OMAiWANFYVN.
OrthoDBiEOG7W9S51.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEYSVIVP AYHEKLNIKP LTTRLFAGMS PEMAKKTELI FVDDNSQDGS
60 70 80 90 100
VEEVDALAHQ GYNVRIIVRT NERGLSSAVL KGFYEAKGQY LVCMDADLQH
110 120 130 140 150
PPETVPKLFE SLHDHAFTLG TRYAPGVGID KDWPMYRRVI SSTARMMARP
160 170 180 190 200
LTIASDPMSG FFGLQKKYLE NCNPRDINSQ GFKIALELLA KLPLPRDPRV
210 220 230 240 250
AIGEVPFTFG VRTEGESKLS GKVIIQYLQQ LKELYVFKFG ANNLILFITF
260
WSILFFYVCY QLYHLVF
Length:267
Mass (Da):30,362
Last modified:January 23, 2007 - v3
Checksum:i14F2F59F7B6A391A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04184 Genomic DNA. Translation: AAA34578.1.
U25842 Genomic DNA. Translation: AAB68116.1.
BK006949 Genomic DNA. Translation: DAA11599.1.
PIRiA32122.
RefSeqiNP_015509.1. NM_001184280.1.

Genome annotation databases

EnsemblFungiiYPR183W; YPR183W; YPR183W.
GeneIDi856313.
KEGGisce:YPR183W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04184 Genomic DNA. Translation: AAA34578.1.
U25842 Genomic DNA. Translation: AAB68116.1.
BK006949 Genomic DNA. Translation: DAA11599.1.
PIRiA32122.
RefSeqiNP_015509.1. NM_001184280.1.

3D structure databases

ProteinModelPortaliP14020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36355. 77 interactions.
DIPiDIP-4087N.
IntActiP14020. 11 interactions.
MINTiMINT-486015.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

PTM databases

iPTMnetiP14020.

Proteomic databases

MaxQBiP14020.
PeptideAtlasiP14020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR183W; YPR183W; YPR183W.
GeneIDi856313.
KEGGisce:YPR183W.

Organism-specific databases

EuPathDBiFungiDB:YPR183W.
SGDiS000006387. DPM1.

Phylogenomic databases

GeneTreeiENSGT00550000075000.
HOGENOMiHOG000283250.
InParanoidiP14020.
KOiK00721.
OMAiWANFYVN.
OrthoDBiEOG7W9S51.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER-7201.
YEAST:YPR183W-MONOMER.
BRENDAi2.4.1.83. 984.
SABIO-RKP14020.

Miscellaneous databases

PROiP14020.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein."
    Orlean P., Albright C., Robbins P.W.
    J. Biol. Chem. 263:17499-17507(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Bienvenut W.V., Peters C.
    Submitted (MAY-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-65; 88-137; 150-166; 184-191; 200-212 AND 223-232, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p."
    Faulhammer F., Konrad G., Brankatschk B., Tahirovic S., Knoedler A., Mayinger P.
    J. Cell Biol. 168:185-191(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SAC1, SUBCELLULAR LOCATION.
  8. "In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase."
    Banerjee D.K., Carrasquillo E.A., Hughey P., Schutzbach J.S., Martinez J.A., Baksi K.
    J. Biol. Chem. 280:4174-4181(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION AT SER-141 BY PKA, MUTAGENESIS OF SER-141.

Entry informationi

Entry nameiDPM1_YEAST
AccessioniPrimary (citable) accession number: P14020
Secondary accession number(s): D6W4I3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1885 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.