P14012 (ARGI_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginase
EC=3.5.3.1

Gene names

Name:	arcA
Synonyms:	arc
Ordered Locus Names:	Atu6018
ORF Names:	AGR_pTi_56

Encoded on

Plasmid pTiC58

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Amino-acid degradation; nopaline degradation.
Subunit structure	Homohexamer Probable.
Induction	By nopaline. But not by L-arginine or L-ornithine.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Arginase		PRO_0000173712
Regions
	Region	144 – 148	5	Substrate binding By similarity
	Region	155 – 157	3	Substrate binding By similarity
Sites
	Metal binding	115	1	Manganese 1 By similarity
	Metal binding	142	1	Manganese 1 By similarity
	Metal binding	142	1	Manganese 2 By similarity
	Metal binding	144	1	Manganese 2 By similarity
	Metal binding	146	1	Manganese 1 By similarity
	Metal binding	244	1	Manganese 1 By similarity
	Metal binding	244	1	Manganese 2 By similarity
	Metal binding	246	1	Manganese 2 By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	289	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14012 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 6719BFF66B80571D	FASTA	324	34,695
	10 20 30 40 50 60 MNGAGEINAS RHRKENELKT CQILGAPVQS GASQPGCLMG PDAFRTAGLT QVLTELGWAV 70 80 90 100 110 120 TDLGDATPTV EPELSHPNSA VKNLDALVGW TRSLSQKALE MARSCDLPVF LGGDHSMSAG 130 140 150 160 170 180 TVSGVAQRTA ELGKEQFVLW LDAHTDLHTL HTTASGNLHG TPVAYYTGQS GFEGLPPLAA 190 200 210 220 230 240 PVNPRNVSMM GIRSVDPEER RRVAEIGVQV ADMRVLDEQG VVRPLEAFLD RVSKVSGRLH 250 260 270 280 290 300 VSLDVDFLDP AIAPAVGTTV PGGATFREAH LIMEMLHDSG LVTSLDLAEL NPFLDERGRT 310 320 ARLITDLASS LFGRRVFDRV TTAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arginase of Agrobacterium Ti plasmid C58. DNA sequence, properties, and comparison with eucaryotic enzymes." Schrell A., Alt-Moerbe J., Lanz T., Schroeder J. Eur. J. Biochem. 184:635-641(1989) [PubMed: 2806247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Noc region of Ti plasmid C58 codes for arginase and ornithine cyclodeaminase." Sans N., Schroeder G., Schroeder J. Eur. J. Biochem. 167:81-87(1987) [PubMed: 3040404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15884 Genomic DNA. Translation: CAA33894.1. Z30316 Genomic DNA. Translation: CAA82964.1. AE007871 Genomic DNA. Translation: AAK90976.2.
PIR	AH3229. S06118.
RefSeq	NP_053430.1. NC_002147.1. NP_396535.2. NC_003065.3.
3D structure databases
ProteinModelPortal	P14012.
ModBase	Search...
Protein-protein interaction databases
STRING	P14012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1137341. 1224186.
GenomeReviews	Gene locus Atu6018 in contig AE007871_GR.
KEGG	atu:Atu6018.
PATRIC	20820262. VBIAgrTum91616_5225.
Phylogenomic databases
eggNOG	COG0010.
HOGENOM	HBG391953.
OMA	EMARSCD.
PhylomeDB	P14012.
ProtClustDB	CLSK2459456.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU6018-MONOMER. MetaCyc:MONOMER-11552.
Family and domain databases
InterPro	IPR014033. Arginase_subgr. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
KO	K01476.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. RocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_AGRT5

Accession

Primary (citable) accession number: P14012

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program