P14010 (GABAT_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-aminobutyrate aminotransferase
EC=2.6.1.19

Alternative name(s):
GABA aminotransferase
Short name=GABA-AT
Gamma-amino-N-butyrate transaminase
Short name=GABA transaminase

Gene names

Name:	gatA
ORF Names:	AN2248

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Deaminates gamma-aminobutyric acid (GABA) to succinate-semialdehyde, which in turn is converted to succinate by the succinate semialdehyde dehydrogenase By similarity. Required for the degradation of GABA, which is important for utilization of GABA as nitrogen source.
Catalytic activity	4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gamma-aminobutyric acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-aminobutyrate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	4-aminobutyrate aminotransferase		PRO_0000120379
Amino acid modifications
	Modified residue	356	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14010 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 97013EF39D66D5E0	FASTA	498	55,489
	10 20 30 40 50 60 MASAFRSSLK LRASARLPAV RTITTTPRLR AAEKPYFPNE PTAPKLATAI PGPKNKAASE 70 80 90 100 110 120 QLNEVFDVRS LNMLADYTKS VGNYIADLDG NMLLDVYAQI ASIPVGYNNP HLLKVAASPE 130 140 150 160 170 180 MATSLINRPA LGNFPSADWA HILKTGILKV APKGLDQVFT AMAGSDANET AYKAAFMYYR 190 200 210 220 230 240 QQQRGGPEKE FTEEEIQSSM LNQTPGSPQL SIMSFKAGFH GRLFGSLSTT RSKPIHKLDI 250 260 270 280 290 300 PAFDWPQAPF PSLKYPLEEH AKENAEEEQR CLQEAERLIK EWHNPVAAII VEPIQSEGGD 310 320 330 340 350 360 NHASPAFFRG LREITKRNNV LFIVDEVQTG VGATGKFWAH DHWNLETPPD MVTFSKKAQT 370 380 390 400 410 420 AGYYFGNPAL RPNKPYRQFN TWMGDPSRAL IFRGIIEEIE RLFLVENTAA TGDYLYSGLE 430 440 450 460 470 480 RLAKQYPEHL QNLRGKGQGT FIAWDTPKRD EFLVKGKGVG INIGGSGQNA VRLRPMLIFQ 490 KHHADILLES IEKIIKQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and molecular characterisation of the amdR controlled gatA gene of Aspergillus nidulans." Richardson I.B., Hurley S.K., Hynes M.J. Mol. Gen. Genet. 217:118-125(1989) [PubMed: 2505051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MH 3010.
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed: 19146970] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15647 Genomic DNA. Translation: CAA33674.1. AACD01000036 Genomic DNA. Translation: EAA63933.1. BN001307 Genomic DNA. Translation: CBF86462.1.
PIR	JQ0197.
RefSeq	XP_659852.1. XM_654760.1.
3D structure databases
ProteinModelPortal	P14010.
ModBase	Search...
Protein-protein interaction databases
STRING	P14010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00008938; CADANIAP00008938; CADANIAG00008938.
GeneID	2875488.
KEGG	ani:AN2248.2.
Phylogenomic databases
OMA	ATGKFWA.
OrthoDB	EOG4HX88H.
PhylomeDB	P14010.
Family and domain databases
InterPro	IPR004631. 4NH2But_aminotransferase_euk. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K13524.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF6. GABAtrns_euk. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00699. GABAtrns_euk. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GABAT_EMENI

Accession

Primary (citable) accession number: P14010
Secondary accession number(s): C8VMU4, Q5BB32

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program