Reviewed, UniProtKB/Swiss-Prot P14005 (PELP_PECCC)

Names and origin

Protein names

Recommended name:
    Periplasmic pectate lyase
    EC=4.2.2.2

Gene names

Name:

pelB

Organism

Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)

Taxonomic identifier

555 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconic acid from pectin: step 2/5.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the polysaccharide lyase 2 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Gene Ontology (GO)
Biological process	pectin catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 568	549	Periplasmic pectate lyase		PRO_0000024910

Sequences

	Sequence		Length	Mass (Da)
	P14005-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 23B88CB34528F8CB	FASTA	568	63,524
	10 20 30 40 50 60 MKRFALSLLA GLVALQASAA TPDRLTIVNQ YVDNVLTKAG DHYHGQSPTP LLADGVDPRT 70 80 90 100 110 120 GKQMEWIFPD GRHAVLSNFS AQQNLMRVLV GLSNLSGNPS YKQRAEAIVK YHFQHYQDES 130 140 150 160 170 180 GLLIWGGHRF VDLKTLQPEG PSEKEMVHEL KNAYPYYDLM FSVDKDATAR FIRGFWNAHV 190 200 210 220 230 240 YDWKIMETSR HGKYGQKIGA LWQSPFEQQP PFFATKGLSF LNAGNDLIYS ASLLYKYNKE 250 260 270 280 290 300 DGALVWAKRL AQQYVLPRDK ATGLGVYQFT QALKRDETTD DADTHSKYGD RAQRQFGPEF 310 320 330 340 350 360 GPTALEGNMM LKGRTSTIYS ENALMQLQLG KDLGAEGKEL LTWTTDGLKA FAKYAYNESD 370 380 390 400 410 420 NTFRPMLANG KDLSNYVLPR DGYYGKKGTV IKPYPADNSF LLSYARAYTV LPDAELWRVA 430 440 450 460 470 480 RGIARAQGLG ELGSAPGKDV KVDLATKNND PYALFALLDL YQASKVKDYL SLAEKVGDNI 490 500 510 520 530 540 ISTRYQNGFF MAEPNRQYAD VDTIEPYALL ALEAAVRNQP QSVAPFLNGA GFTEGGYRME 550 560 DGSTRVSTRD NEIFLLNVGE TLKPNNKK

References

[1]

"Extracellular and periplasmic isoenzymes of pectate lyase from Erwinia carotovora subspecies carotovora belong to different gene families." Hinton J.C.D., Sidebotham J.M., Gill D.R., Salmond G.P.C. Mol. Microbiol. 3:1785-1795(1989) [PubMed: 2695748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SCRI 193.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16397 Genomic DNA. Translation: CAA34432.1.
PIR	WZWCCC. S07653.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL2. Polysaccharide Lyase Family 2.
Enzyme and pathway databases
BRENDA	4.2.2.2. 290410.
Family and domain databases
InterPro	IPR010702. Pectate_lyase_2. [Graphical view]
Pfam	PF06917. Pectate_lyase_2. 1 hit. [Graphical view]
PIRSF	PIRSF001432. Pect_lyase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PELP_PECCC

Accession

Primary (citable) accession number: P14005

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)