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Protein

Protein hairy

Gene

h

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pair-rule protein that regulates embryonic segmentation and adult bristle patterning. Transcriptional repressor of genes that require a bHLH protein for their transcription (e.g. the Fushi tarazu gene).1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • E-box binding Source: FlyBase
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: FlyBase
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • cell morphogenesis Source: FlyBase
  • membrane organization Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • nervous system development Source: FlyBase
  • open tracheal system development Source: FlyBase
  • periodic partitioning by pair rule gene Source: FlyBase
  • posterior head segmentation Source: FlyBase
  • R8 cell fate commitment Source: FlyBase
  • regulation of cellular metabolic process Source: FlyBase
  • response to hypoxia Source: FlyBase
  • salivary gland development Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • trunk segmentation Source: FlyBase
  • tube morphogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Pair-rule protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP14003.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein hairy
Gene namesi
Name:h
ORF Names:CG6494
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001168. h.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271811 – 337Protein hairyAdd BLAST337

Post-translational modificationi

Ubiquitinated by Topors.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP14003.
PRIDEiP14003.

Expressioni

Gene expression databases

BgeeiFBgn0001168.
GenevisibleiP14003. DM.

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein (Groucho). Interacts with gro (via WPRW motif) and Topors.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CtBPO460364EBI-123011,EBI-159330
dgrnQ9VNJ05EBI-123011,EBI-186615
groP163713EBI-123011,EBI-153866
ToporsQ9V8P94EBI-123011,EBI-147805
UbxP839493EBI-123011,EBI-202590

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi64402. 11 interactors.
DIPiDIP-637N.
IntActiP14003. 8 interactors.
MINTiMINT-1542874.
STRINGi7227.FBpp0099504.

Structurei

3D structure databases

ProteinModelPortaliP14003.
SMRiP14003.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 88bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini107 – 136OrangePROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 48Interaction with ToporsAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi334 – 337WRPW motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 157Gln-rich9
Compositional biasi222 – 237Gln-richAdd BLAST16
Compositional biasi241 – 250Poly-Ala10

Domaini

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00700000104168.
InParanoidiP14003.
KOiK09090.
OMAiEEQPWRP.
OrthoDBiEOG091G0GBN.
PhylomeDBiP14003.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTGVTAANM TNVLGTAVVP AQLKETPLKS DRRSNKPIME KRRRARINNC
60 70 80 90 100
LNELKTLILD ATKKDPARHS KLEKADILEK TVKHLQELQR QQAAMQQAAD
110 120 130 140 150
PKIVNKFKAG FADCVNEVSR FPGIEPAQRR RLLQHLSNCI NGVKTELHQQ
160 170 180 190 200
QRQQQQQSIH AQMLPSPPSS PEQDSQQGAA APYLFGIQQT ASGYFLPNGM
210 220 230 240 250
QVIPTKLPNG SIALVLPQSL PQQQQQQLLQ HQQQQQQLAV AAAAAAAAAA
260 270 280 290 300
QQQPMLVSMP QRTASTGSAS SHSSAGYESA PGSSSSCSYA PPSPANSSYE
310 320 330
PMDIKPSVIQ RVPMEQQPLS LVIKKQIKEE EQPWRPW
Length:337
Mass (Da):37,005
Last modified:April 18, 2006 - v2
Checksum:i49BECAF7F2D69FC4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti21A → S in strain: R3-6, R3-105 and R3-107. 1 Publication1
Natural varianti292P → S.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15904 Genomic DNA. Translation: CAA34018.1.
X15905 mRNA. Translation: CAA34019.1.
AY055833 Genomic DNA. Translation: AAL17767.1.
AY055834 Genomic DNA. Translation: AAL17768.1.
AY055835 Genomic DNA. Translation: AAL17769.1.
AY055836 Genomic DNA. Translation: AAL17770.1.
AY055837 Genomic DNA. Translation: AAL17771.1.
AY055838 Genomic DNA. Translation: AAL17772.1.
AY055839 Genomic DNA. Translation: AAL17773.1.
AY055840 Genomic DNA. Translation: AAL17774.1.
AY055841 Genomic DNA. Translation: AAL17775.1.
AY055842 Genomic DNA. Translation: AAL17776.1.
AE014296 Genomic DNA. Translation: AAF50378.1.
AE014296 Genomic DNA. Translation: AAX52752.1.
AY119633 mRNA. Translation: AAM50287.1.
PIRiS06956.
RefSeqiNP_001014577.1. NM_001014577.2.
NP_523977.2. NM_079253.4.
UniGeneiDm.2554.

Genome annotation databases

EnsemblMetazoaiFBtr0076569; FBpp0076296; FBgn0001168.
FBtr0100153; FBpp0099504; FBgn0001168.
GeneIDi38995.
KEGGidme:Dmel_CG6494.
UCSCiCG6494-RA. d. melanogaster.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15904 Genomic DNA. Translation: CAA34018.1.
X15905 mRNA. Translation: CAA34019.1.
AY055833 Genomic DNA. Translation: AAL17767.1.
AY055834 Genomic DNA. Translation: AAL17768.1.
AY055835 Genomic DNA. Translation: AAL17769.1.
AY055836 Genomic DNA. Translation: AAL17770.1.
AY055837 Genomic DNA. Translation: AAL17771.1.
AY055838 Genomic DNA. Translation: AAL17772.1.
AY055839 Genomic DNA. Translation: AAL17773.1.
AY055840 Genomic DNA. Translation: AAL17774.1.
AY055841 Genomic DNA. Translation: AAL17775.1.
AY055842 Genomic DNA. Translation: AAL17776.1.
AE014296 Genomic DNA. Translation: AAF50378.1.
AE014296 Genomic DNA. Translation: AAX52752.1.
AY119633 mRNA. Translation: AAM50287.1.
PIRiS06956.
RefSeqiNP_001014577.1. NM_001014577.2.
NP_523977.2. NM_079253.4.
UniGeneiDm.2554.

3D structure databases

ProteinModelPortaliP14003.
SMRiP14003.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64402. 11 interactors.
DIPiDIP-637N.
IntActiP14003. 8 interactors.
MINTiMINT-1542874.
STRINGi7227.FBpp0099504.

Proteomic databases

PaxDbiP14003.
PRIDEiP14003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076569; FBpp0076296; FBgn0001168.
FBtr0100153; FBpp0099504; FBgn0001168.
GeneIDi38995.
KEGGidme:Dmel_CG6494.
UCSCiCG6494-RA. d. melanogaster.

Organism-specific databases

CTDi38995.
FlyBaseiFBgn0001168. h.

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00700000104168.
InParanoidiP14003.
KOiK09090.
OMAiEEQPWRP.
OrthoDBiEOG091G0GBN.
PhylomeDBiP14003.

Enzyme and pathway databases

SignaLinkiP14003.

Miscellaneous databases

GenomeRNAii38995.
PROiP14003.

Gene expression databases

BgeeiFBgn0001168.
GenevisibleiP14003. DM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHAIR_DROME
AccessioniPrimary (citable) accession number: P14003
Secondary accession number(s): A4V1N7
, Q95NH3, Q95NU9, Q9VSN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 18, 2006
Last modified: November 2, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.