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Protein

Protein hairy

Gene

h

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pair-rule protein that regulates embryonic segmentation and adult bristle patterning. Transcriptional repressor of genes that require a bHLH protein for their transcription (e.g. the Fushi tarazu gene).1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • E-box binding Source: FlyBase
  • protein dimerization activity Source: InterPro
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • cell morphogenesis Source: FlyBase
  • membrane organization Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • nervous system development Source: FlyBase
  • open tracheal system development Source: FlyBase
  • periodic partitioning by pair rule gene Source: FlyBase
  • posterior head segmentation Source: FlyBase
  • R8 cell fate commitment Source: FlyBase
  • regulation of cellular metabolic process Source: FlyBase
  • response to hypoxia Source: FlyBase
  • salivary gland development Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • trunk segmentation Source: FlyBase
  • tube morphogenesis Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Pair-rule protein, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiP14003

Names & Taxonomyi

Protein namesi
Recommended name:
Protein hairy
Gene namesi
Name:h
ORF Names:CG6494
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001168 h

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271811 – 337Protein hairyAdd BLAST337

Post-translational modificationi

Ubiquitinated by Topors.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP14003
PRIDEiP14003

Expressioni

Gene expression databases

BgeeiFBgn0001168
GenevisibleiP14003 DM

Interactioni

Subunit structurei

Transcription repression requires formation of a complex with a corepressor protein (Groucho). Interacts with gro (via WPRW motif) and Topors.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi64402, 34 interactors
DIPiDIP-637N
ELMiP14003
IntActiP14003, 8 interactors
MINTiP14003
STRINGi7227.FBpp0099504

Structurei

3D structure databases

ProteinModelPortaliP14003
SMRiP14003
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 88bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini107 – 136OrangePROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 48Interaction with ToporsAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi334 – 337WRPW motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 157Gln-rich9
Compositional biasi222 – 237Gln-richAdd BLAST16
Compositional biasi241 – 250Poly-Ala10

Domaini

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.

Phylogenomic databases

eggNOGiKOG4304 Eukaryota
ENOG4111F0X LUCA
GeneTreeiENSGT00700000104168
InParanoidiP14003
KOiK09090
OMAiEQPWRPW
OrthoDBiEOG091G0GBN
PhylomeDBiP14003

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003650 Orange_dom
PfamiView protein in Pfam
PF07527 Hairy_orange, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00511 ORANGE, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS51054 ORANGE, 1 hit

Sequencei

Sequence statusi: Complete.

P14003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTGVTAANM TNVLGTAVVP AQLKETPLKS DRRSNKPIME KRRRARINNC
60 70 80 90 100
LNELKTLILD ATKKDPARHS KLEKADILEK TVKHLQELQR QQAAMQQAAD
110 120 130 140 150
PKIVNKFKAG FADCVNEVSR FPGIEPAQRR RLLQHLSNCI NGVKTELHQQ
160 170 180 190 200
QRQQQQQSIH AQMLPSPPSS PEQDSQQGAA APYLFGIQQT ASGYFLPNGM
210 220 230 240 250
QVIPTKLPNG SIALVLPQSL PQQQQQQLLQ HQQQQQQLAV AAAAAAAAAA
260 270 280 290 300
QQQPMLVSMP QRTASTGSAS SHSSAGYESA PGSSSSCSYA PPSPANSSYE
310 320 330
PMDIKPSVIQ RVPMEQQPLS LVIKKQIKEE EQPWRPW
Length:337
Mass (Da):37,005
Last modified:April 18, 2006 - v2
Checksum:i49BECAF7F2D69FC4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti21A → S in strain: R3-6, R3-105 and R3-107. 1 Publication1
Natural varianti292P → S1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15904 Genomic DNA Translation: CAA34018.1
X15905 mRNA Translation: CAA34019.1
AY055833 Genomic DNA Translation: AAL17767.1
AY055834 Genomic DNA Translation: AAL17768.1
AY055835 Genomic DNA Translation: AAL17769.1
AY055836 Genomic DNA Translation: AAL17770.1
AY055837 Genomic DNA Translation: AAL17771.1
AY055838 Genomic DNA Translation: AAL17772.1
AY055839 Genomic DNA Translation: AAL17773.1
AY055840 Genomic DNA Translation: AAL17774.1
AY055841 Genomic DNA Translation: AAL17775.1
AY055842 Genomic DNA Translation: AAL17776.1
AE014296 Genomic DNA Translation: AAF50378.1
AE014296 Genomic DNA Translation: AAX52752.1
AY119633 mRNA Translation: AAM50287.1
PIRiS06956
RefSeqiNP_001014577.1, NM_001014577.2
NP_523977.2, NM_079253.4
UniGeneiDm.2554

Genome annotation databases

EnsemblMetazoaiFBtr0076569; FBpp0076296; FBgn0001168
FBtr0100153; FBpp0099504; FBgn0001168
GeneIDi38995
KEGGidme:Dmel_CG6494
UCSCiCG6494-RA d. melanogaster

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHAIR_DROME
AccessioniPrimary (citable) accession number: P14003
Secondary accession number(s): A4V1N7
, Q95NH3, Q95NU9, Q9VSN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 18, 2006
Last modified: May 23, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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