P14003 (HAIR_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein hairy

Gene names

Name:	h
ORF Names:	CG6494

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pair-rule protein that regulates embryonic segmentation and adult bristle patterning. Transcriptional repressor of genes that require a bHLH protein for their transcription (e.g. the Fushi tarazu gene). Ref.7
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein (Groucho). Interacts with gro (via WPRW motif) and Topors. Ref.7 Ref.8
Subcellular location	Nucleus.
Domain	Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). Ref.6 Ref.7 The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins. Ref.6 Ref.7
Post-translational modification	Ubiquitinated by Topors. Ref.8
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Developmental protein Pair-rule protein Repressor
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	R8 cell fate commitment Non-traceable author statement. Source: FlyBase cell morphogenesis Inferred from mutant phenotype. Source: FlyBase cellular membrane organization Traceable author statement. Source: FlyBase open tracheal system development Inferred from mutant phenotype. Source: FlyBase periodic partitioning by pair rule gene Non-traceable author statement. Source: FlyBase posterior head segmentation Traceable author statement. Source: FlyBase response to hypoxia Inferred from mutant phenotype. Source: FlyBase salivary gland morphogenesis Inferred from mutant phenotype. Source: FlyBase trunk segmentation Traceable author statement. Source: FlyBase tube morphogenesis Inferred from mutant phenotype. Source: FlyBase
Cellular component	nucleus Inferred by curator Ref.7. Source: UniProtKB
Molecular function	E-box binding Inferred from direct assay. Source: FlyBase RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay. Source: FlyBase RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction Ref.8Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CtBP	O46036	2	EBI-123011,EBI-159330
Topors	Q9V8P9	4	EBI-123011,EBI-147805
Ubx	P83949	3	EBI-123011,EBI-202590

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Protein hairy		PRO_0000127181
Regions
	Domain	45 – 89	45	Helix-loop-helix motif
	Domain	107 – 136	30	Orange
	DNA binding	32 – 44	13	Basic motif Ref.8
	Region	29 – 48	20	Interaction with Topors
	Motif	334 – 337	4	WRPW motif
	Compositional bias	149 – 157	9	Gln-rich
	Compositional bias	222 – 237	16	Gln-rich
	Compositional bias	241 – 250	10	Poly-Ala
Natural variations
	Natural variant	21	1	A → S in strain: R3-6, R3-105 and R3-107. Ref.2
	Natural variant	292	1	P → S. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14003 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 49BECAF7F2D69FC4	FASTA	337	37,005
	10 20 30 40 50 60 MVTGVTAANM TNVLGTAVVP AQLKETPLKS DRRSNKPIME KRRRARINNC LNELKTLILD 70 80 90 100 110 120 ATKKDPARHS KLEKADILEK TVKHLQELQR QQAAMQQAAD PKIVNKFKAG FADCVNEVSR 130 140 150 160 170 180 FPGIEPAQRR RLLQHLSNCI NGVKTELHQQ QRQQQQQSIH AQMLPSPPSS PEQDSQQGAA 190 200 210 220 230 240 APYLFGIQQT ASGYFLPNGM QVIPTKLPNG SIALVLPQSL PQQQQQQLLQ HQQQQQQLAV 250 260 270 280 290 300 AAAAAAAAAA QQQPMLVSMP QRTASTGSAS SHSSAGYESA PGSSSSCSYA PPSPANSSYE 310 320 330 PMDIKPSVIQ RVPMEQQPLS LVIKKQIKEE EQPWRPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila hairy protein acts in both segmentation and bristle patterning and shows homology to N-myc." Rushlow C.A., Hogan A., Pierchin S.M., Howe K.M., Lardelli M., Ish-Horowicz D. EMBO J. 8:3095-3103(1989) [PubMed: 2479541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT SER-292. Strain: Oregon-R.
[2]	"hairy. A quantitative trait locus for Drosophila sensory bristle number." Robin C., Lyman R.F., Long A.D., Langley C.H., Mackay T.F.C. Genetics 162:155-164(2002) [PubMed: 12242230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-21. Strain: R3-105, R3-107, R3-19, R3-2, R3-24, R3-48, R3-53, R3-6, R3-74 and R3-95.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins." Paroush Z., Finley R.L. Jr., Kidd T., Wainwright S.M., Ingham P.W., Brent R., Ish-Horowicz D. Cell 79:805-815(1994) [PubMed: 8001118] [Abstract] Cited for: DOMAIN WRPW MOTIF.
[7]	"The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain." Fisher A.L., Ohsako S., Caudy M. Mol. Cell. Biol. 16:2670-2677(1996) [PubMed: 8649374] [Abstract] Cited for: FUNCTION, INTERACTION WITH GRO, DOMAIN WRPW MOTIF.
[8]	"Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein." Secombe J., Parkhurst S.M. J. Biol. Chem. 279:17126-17133(2004) [PubMed: 14871887] [Abstract] Cited for: DNA-BINDING, INTERACTION WITH TOPORS, UBIQUITINATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15904 Genomic DNA. Translation: CAA34018.1. X15905 mRNA. Translation: CAA34019.1. AY055833 Genomic DNA. Translation: AAL17767.1. AY055834 Genomic DNA. Translation: AAL17768.1. AY055835 Genomic DNA. Translation: AAL17769.1. AY055836 Genomic DNA. Translation: AAL17770.1. AY055837 Genomic DNA. Translation: AAL17771.1. AY055838 Genomic DNA. Translation: AAL17772.1. AY055839 Genomic DNA. Translation: AAL17773.1. AY055840 Genomic DNA. Translation: AAL17774.1. AY055841 Genomic DNA. Translation: AAL17775.1. AY055842 Genomic DNA. Translation: AAL17776.1. AE014296 Genomic DNA. Translation: AAF50378.1. AE014296 Genomic DNA. Translation: AAX52752.1. AY119633 mRNA. Translation: AAM50287.1.
PIR	S06956.
RefSeq	NP_001014577.1. NM_001014577.1. NP_523977.2. NM_079253.3.
UniGene	Dm.2554.
3D structure databases
ProteinModelPortal	P14003.
SMR	P14003. Positions 29-92, 98-143.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-32713N. DIP-637N.
IntAct	P14003. 9 interactions.
STRING	P14003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076569; FBpp0076296; FBgn0001168. FBtr0100153; FBpp0099504; FBgn0001168.
GeneID	38995.
KEGG	dme:Dmel_CG6494.
NMPDR	fig|7227.3.peg.8919.
UCSC	CG6494-RA. d. melanogaster.
Organism-specific databases
CTD	38995.
FlyBase	FBgn0001168. h.
Phylogenomic databases
eggNOG	inNOG09379.
GeneTree	EMGT00050000004786.
InParanoid	P14003.
OMA	NGMQVIP.
OrthoDB	EOG4M37RH.
PhylomeDB	P14003.
Gene expression databases
Bgee	P14003.
GermOnline	CG6494. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. IPR003650. Orange. IPR018352. Orange_subgr. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
KO	K09090.
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	811370.

Entry information

Entry name

HAIR_DROME

Accession

Primary (citable) accession number: P14003
Secondary accession number(s): A4V1N7 , Q95NH3, Q95NU9, Q9VSN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project