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P14002 (BGLB_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thermostable beta-glucosidase B

EC=3.2.1.21
Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase
Gene names
Name:bglB
Ordered Locus Names:Cthe_1256
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Pathway

Glycan metabolism; cellulose degradation.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 755755Thermostable beta-glucosidase B
PRO_0000210778

Sites

Active site2311 By similarity

Experimental info

Sequence conflict1251S → P in CAA33665. Ref.1
Sequence conflict2871F → I in CAA33665. Ref.1
Sequence conflict300 – 3012DK → EQ in CAA33665. Ref.1
Sequence conflict3711G → A in CAA33665. Ref.1
Sequence conflict481 – 49313ALADV…EVNPS → RWRMCYSVKSIV in CAA33665. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14002 [UniParc].

Last modified April 17, 2007. Version 2.
Checksum: 944A4D782E6AEAB8

FASTA75583,900
        10         20         30         40         50         60 
MAVDIKKIIK QMTLEEKAGL CSGLDFWHTK PVERLGIPSI MMTDGPHGLR KQREDAEIAD 

        70         80         90        100        110        120 
INNSVPATCF PSAAGLACSW DRELVERVGA ALGEECQAEN VSILLGPGAN IKRSPLCGRN 

       130        140        150        160        170        180 
FEYFSEDPYL SSELAASHIK GVQSQGVGAC LKHFAANNQE HRRMTVDTIV DERTLREIYF 

       190        200        210        220        230        240 
ASFENAVKKA RPWVVMCAYN KLNGEYCSEN RYLLTEVLKN EWMHDGFVVS DWGAVNDRVS 

       250        260        270        280        290        300 
GLDAGLDLEM PTSHGITDKK IVEAVKSGKL SENILNRAVE RILKVIFMAL ENKKENAQYD 

       310        320        330        340        350        360 
KDAHHRLARQ AAAESMVLLK NEDDVLPLKK SGTIALIGAF VKKPRYQGSG SSHITPTRLD 

       370        380        390        400        410        420 
DIYEEIKKAG GDKVNLVYSE GYRLENDGID EELINEAKKA ASSSDVAVVF AGLPDEYESE 

       430        440        450        460        470        480 
GFDRTHMSIP ENQNRLIEAV AEVQSNIVVV LLNGSPVEMP WIDKVKSVLE AYLGGQALGG 

       490        500        510        520        530        540 
ALADVLFGEV NPSGKLAETF PVKLSHNPSY LNFPGEDDRV EYKEGLFVGY RYYDTKGIEP 

       550        560        570        580        590        600 
LFPFGHGLSY TKFEYSDISV DKKDVSDNSI INVSVKVKNV GKMAGKEIVQ LYVKDVKSSV 

       610        620        630        640        650        660 
RRPEKELKGF EKVFLNPGEE KTVTFTLDKR AFAYYNTQIK DWHVESGEFL ILIGRSSRDI 

       670        680        690        700        710        720 
VLKESVRVNS TVKIRKRFTV NSAVEDVMSD SSAAAVLGPV LKEITDALQI DMDNAHDMMA 

       730        740        750 
ANIKNMPLRS LVGYSQGRLS EEMLEELVDK INNVE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the Clostridium thermocellum bgIB gene encoding thermostable beta-glucosidase B: homology to fungal beta-glucosidases."
Graebnitz F., Ruecknagel K.P., Seiss M., Staudenbauer W.L.
Mol. Gen. Genet. 217:70-76(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15644 Genomic DNA. Translation: CAA33665.1.
CP000568 Genomic DNA. Translation: ABN52488.1.
PIRS04381.
RefSeqYP_001037681.1. NC_009012.1.

3D structure databases

ProteinModelPortalP14002.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_1256.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN52488; ABN52488; Cthe_1256.
GeneID4809761.
KEGGcth:Cthe_1256.
PATRIC19516291. VBICloThe47081_1326.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000031215.
KOK05349.
OMAYYTEGIF.
OrthoDBEOG6N9468.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-1306-MONOMER.
UniPathwayUPA00696.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR019800. Glyco_hydro_3_AS.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR30620. PTHR30620. 1 hit.
PfamPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSPR00133. GLHYDRLASE3.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGLB_CLOTH
AccessionPrimary (citable) accession number: P14002
Secondary accession number(s): A3DEV9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries