P14002 (BGLB_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thermostable beta-glucosidase B
EC=3.2.1.21

Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase

Gene names

Name:	bglB
Ordered Locus Names:	Cthe_1256

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 755	755	Thermostable beta-glucosidase B		PRO_0000210778
Sites
	Active site	231	1	By similarity
Experimental info
	Sequence conflict	125	1	S → P in CAA33665. Ref.1
	Sequence conflict	287	1	F → I in CAA33665. Ref.1
	Sequence conflict	300 – 301	2	DK → EQ in CAA33665. Ref.1
	Sequence conflict	371	1	G → A in CAA33665. Ref.1
	Sequence conflict	481 – 493	13	ALADV…EVNPS → RWRMCYSVKSIV in CAA33665. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14002 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: 944A4D782E6AEAB8	FASTA	755	83,900
	10 20 30 40 50 60 MAVDIKKIIK QMTLEEKAGL CSGLDFWHTK PVERLGIPSI MMTDGPHGLR KQREDAEIAD 70 80 90 100 110 120 INNSVPATCF PSAAGLACSW DRELVERVGA ALGEECQAEN VSILLGPGAN IKRSPLCGRN 130 140 150 160 170 180 FEYFSEDPYL SSELAASHIK GVQSQGVGAC LKHFAANNQE HRRMTVDTIV DERTLREIYF 190 200 210 220 230 240 ASFENAVKKA RPWVVMCAYN KLNGEYCSEN RYLLTEVLKN EWMHDGFVVS DWGAVNDRVS 250 260 270 280 290 300 GLDAGLDLEM PTSHGITDKK IVEAVKSGKL SENILNRAVE RILKVIFMAL ENKKENAQYD 310 320 330 340 350 360 KDAHHRLARQ AAAESMVLLK NEDDVLPLKK SGTIALIGAF VKKPRYQGSG SSHITPTRLD 370 380 390 400 410 420 DIYEEIKKAG GDKVNLVYSE GYRLENDGID EELINEAKKA ASSSDVAVVF AGLPDEYESE 430 440 450 460 470 480 GFDRTHMSIP ENQNRLIEAV AEVQSNIVVV LLNGSPVEMP WIDKVKSVLE AYLGGQALGG 490 500 510 520 530 540 ALADVLFGEV NPSGKLAETF PVKLSHNPSY LNFPGEDDRV EYKEGLFVGY RYYDTKGIEP 550 560 570 580 590 600 LFPFGHGLSY TKFEYSDISV DKKDVSDNSI INVSVKVKNV GKMAGKEIVQ LYVKDVKSSV 610 620 630 640 650 660 RRPEKELKGF EKVFLNPGEE KTVTFTLDKR AFAYYNTQIK DWHVESGEFL ILIGRSSRDI 670 680 690 700 710 720 VLKESVRVNS TVKIRKRFTV NSAVEDVMSD SSAAAVLGPV LKEITDALQI DMDNAHDMMA 730 740 750 ANIKNMPLRS LVGYSQGRLS EEMLEELVDK INNVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Clostridium thermocellum bgIB gene encoding thermostable beta-glucosidase B: homology to fungal beta-glucosidases." Graebnitz F., Ruecknagel K.P., Seiss M., Staudenbauer W.L. Mol. Gen. Genet. 217:70-76(1989) [PubMed: 2505054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15644 Genomic DNA. Translation: CAA33665.1. CP000568 Genomic DNA. Translation: ABN52488.1.
PIR	S04381.
RefSeq	YP_001037681.1. NC_009012.1.
3D structure databases
ProteinModelPortal	P14002.
ModBase	Search...
Protein-protein interaction databases
STRING	P14002.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4809761.
GenomeReviews	Gene locus Cthe_1256 in contig CP000568_GR.
KEGG	cth:Cthe_1256.
NMPDR	fig|203119.1.peg.2216.
PATRIC	19516291. VBICloThe47081_1326.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HBG749096.
OMA	QEHRRMT.
ProtClustDB	CLSK965056.
Family and domain databases
InterPro	IPR019800. Glyco_hydro_3_AS. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.40.50.1700. G3DSA:3.40.50.1700. 1 hit. G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
KO	K05349.
Pfam	PF00933. Glyco_hydro_3. 1 hit. PF01915. Glyco_hydro_3_C. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
SUPFAM	SSF52279. Glyco_hydro_3_C. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLB_CLOTH

Accession

Primary (citable) accession number: P14002
Secondary accession number(s): A3DEV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program