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Protein

Thermostable beta-glucosidase B

Gene

bglB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Pathway: cellulose degradation

This protein is involved in the pathway cellulose degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway cellulose degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei231 – 2311By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-1306-MONOMER.
UniPathwayiUPA00696.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable beta-glucosidase B (EC:3.2.1.21)
Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase
Gene namesi
Name:bglB
Ordered Locus Names:Cthe_1256
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 755755Thermostable beta-glucosidase BPRO_0000210778Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_1256.

Structurei

3D structure databases

ProteinModelPortaliP14002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family.Curated

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000031215.
KOiK05349.
OMAiAMVNGVE.
OrthoDBiEOG6N9468.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR019800. Glyco_hydro_3_AS.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDIKKIIK QMTLEEKAGL CSGLDFWHTK PVERLGIPSI MMTDGPHGLR
60 70 80 90 100
KQREDAEIAD INNSVPATCF PSAAGLACSW DRELVERVGA ALGEECQAEN
110 120 130 140 150
VSILLGPGAN IKRSPLCGRN FEYFSEDPYL SSELAASHIK GVQSQGVGAC
160 170 180 190 200
LKHFAANNQE HRRMTVDTIV DERTLREIYF ASFENAVKKA RPWVVMCAYN
210 220 230 240 250
KLNGEYCSEN RYLLTEVLKN EWMHDGFVVS DWGAVNDRVS GLDAGLDLEM
260 270 280 290 300
PTSHGITDKK IVEAVKSGKL SENILNRAVE RILKVIFMAL ENKKENAQYD
310 320 330 340 350
KDAHHRLARQ AAAESMVLLK NEDDVLPLKK SGTIALIGAF VKKPRYQGSG
360 370 380 390 400
SSHITPTRLD DIYEEIKKAG GDKVNLVYSE GYRLENDGID EELINEAKKA
410 420 430 440 450
ASSSDVAVVF AGLPDEYESE GFDRTHMSIP ENQNRLIEAV AEVQSNIVVV
460 470 480 490 500
LLNGSPVEMP WIDKVKSVLE AYLGGQALGG ALADVLFGEV NPSGKLAETF
510 520 530 540 550
PVKLSHNPSY LNFPGEDDRV EYKEGLFVGY RYYDTKGIEP LFPFGHGLSY
560 570 580 590 600
TKFEYSDISV DKKDVSDNSI INVSVKVKNV GKMAGKEIVQ LYVKDVKSSV
610 620 630 640 650
RRPEKELKGF EKVFLNPGEE KTVTFTLDKR AFAYYNTQIK DWHVESGEFL
660 670 680 690 700
ILIGRSSRDI VLKESVRVNS TVKIRKRFTV NSAVEDVMSD SSAAAVLGPV
710 720 730 740 750
LKEITDALQI DMDNAHDMMA ANIKNMPLRS LVGYSQGRLS EEMLEELVDK

INNVE
Length:755
Mass (Da):83,900
Last modified:April 17, 2007 - v2
Checksum:i944A4D782E6AEAB8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251S → P in CAA33665 (PubMed:2505054).Curated
Sequence conflicti287 – 2871F → I in CAA33665 (PubMed:2505054).Curated
Sequence conflicti300 – 3012DK → EQ in CAA33665 (PubMed:2505054).Curated
Sequence conflicti371 – 3711G → A in CAA33665 (PubMed:2505054).Curated
Sequence conflicti481 – 49313ALADV…EVNPS → RWRMCYSVKSIV in CAA33665 (PubMed:2505054).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15644 Genomic DNA. Translation: CAA33665.1.
CP000568 Genomic DNA. Translation: ABN52488.1.
PIRiS04381.
RefSeqiWP_003517480.1. NC_009012.1.
YP_001037681.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN52488; ABN52488; Cthe_1256.
KEGGicth:Cthe_1256.
PATRICi19516291. VBICloThe47081_1326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15644 Genomic DNA. Translation: CAA33665.1.
CP000568 Genomic DNA. Translation: ABN52488.1.
PIRiS04381.
RefSeqiWP_003517480.1. NC_009012.1.
YP_001037681.1. NC_009012.1.

3D structure databases

ProteinModelPortaliP14002.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_1256.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN52488; ABN52488; Cthe_1256.
KEGGicth:Cthe_1256.
PATRICi19516291. VBICloThe47081_1326.

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000031215.
KOiK05349.
OMAiAMVNGVE.
OrthoDBiEOG6N9468.

Enzyme and pathway databases

UniPathwayiUPA00696.
BioCyciCTHE203119:GIW8-1306-MONOMER.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR019800. Glyco_hydro_3_AS.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Clostridium thermocellum bgIB gene encoding thermostable beta-glucosidase B: homology to fungal beta-glucosidases."
    Graebnitz F., Ruecknagel K.P., Seiss M., Staudenbauer W.L.
    Mol. Gen. Genet. 217:70-76(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiBGLB_CLOTH
AccessioniPrimary (citable) accession number: P14002
Secondary accession number(s): A3DEV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 17, 2007
Last modified: May 27, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.