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Protein

Transposon Tn7 transposition protein TnsA

Gene

tnsA

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and TnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas TnsABC and TnsD promote low-frequency insertion into many different sites.2 Publications

Cofactori

a divalent metal cation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi90 – 10819H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • nuclease activity Source: InterPro
  • transposase activity Source: UniProtKB

GO - Biological processi

  • transposition, DNA-mediated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA recombination, Transposition

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Tn7 transposition protein TnsA
Gene namesi
Name:tnsA
Encoded oniPlasmid R7211 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic chromosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281D → A: No effect on recombination. 1 Publication
Mutagenesisi34 – 341T → A: No effect on recombination. 1 Publication
Mutagenesisi37 – 371E → A: Modest decrease in 5'-cleavage. 1 Publication
Mutagenesisi57 – 571H → A: Prevents recombination, may effect protein structure. 1 Publication
Mutagenesisi60 – 601S → A: Prevents recombination, may effect protein structure. 1 Publication
Mutagenesisi108 – 1081D → A: No effect on recombination. 1 Publication
Mutagenesisi114 – 1141D → A: Prevents 5'-cleavage. 1 Publication
Mutagenesisi144 – 1441E → A: No effect on recombination. 1 Publication
Mutagenesisi147 – 1471E → A: No effect on recombination. 1 Publication
Mutagenesisi149 – 1491E → A: Decrease in 5' cleavage. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Transposon Tn7 transposition protein TnsAPRO_0000072611Add
BLAST

Proteomic databases

PRIDEiP13988.

Interactioni

Protein-protein interaction databases

IntActiP13988. 1 interaction.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 189Combined sources
Turni19 – 224Combined sources
Helixi26 – 283Combined sources
Helixi35 – 373Combined sources
Beta strandi45 – 484Combined sources
Turni50 – 523Combined sources
Beta strandi53 – 586Combined sources
Helixi61 – 7212Combined sources
Beta strandi76 – 849Combined sources
Helixi87 – 9711Combined sources
Beta strandi112 – 12312Combined sources
Beta strandi125 – 1317Combined sources
Helixi134 – 1385Combined sources
Helixi140 – 15617Combined sources
Beta strandi160 – 1634Combined sources
Helixi165 – 1673Combined sources
Helixi170 – 18011Combined sources
Helixi189 – 1924Combined sources
Helixi195 – 20410Combined sources
Turni205 – 2073Combined sources
Helixi210 – 22011Combined sources
Helixi227 – 23711Combined sources
Beta strandi240 – 2423Combined sources
Helixi249 – 2513Combined sources
Helixi254 – 2563Combined sources
Beta strandi262 – 2643Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F1ZX-ray2.40A/B1-273[»]
1T0FX-ray1.85A/B1-273[»]
ProteinModelPortaliP13988.
SMRiP13988. Positions 5-268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13988.

Family & Domainsi

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.1350.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR014832. TnsA_C.
IPR014833. TnsA_N.
IPR011856. tRNA_endonuc-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08721. Tn7_Tnp_TnsA_C. 1 hit.
PF08722. Tn7_Tnp_TnsA_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P13988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKANSSFSE VQIARRIKEG RGQGHGKDYI PWLTVQEVPS SGRSHRIYSH
60 70 80 90 100
KTGRVHHLLS DLELAVFLSL EWESSVLDIR EQFPLLPSDT RQIAIDSGIK
110 120 130 140 150
HPVIRGVDQV MSTDFLVDCK DGPFEQFAIQ VKPAAALQDE RTLEKLELER
160 170 180 190 200
RYWQQKQIPW FIFTDKEINP VVKENIEWLY SVKTEEVSAE LLAQLSPLAH
210 220 230 240 250
ILQEKGDENI INVCKQVDIA YDLELGKTLS EIRALTANGF IKFNIYKSFR
260 270
ANKCADLCIS QVVNMEELRY VAN
Length:273
Mass (Da):31,257
Last modified:January 1, 1990 - v1
Checksum:i2C2EFDE9A7D9C477
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391D → G in AAA24680 (Ref. 2) Curated
Sequence conflicti227 – 2271K → Q in AAA24680 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17693 Genomic DNA. Translation: CAA35683.1.
M37391 Genomic DNA. Translation: AAA24680.1.
AP002527 Genomic DNA. Translation: BAB12613.1.
X03474 Genomic DNA. Translation: CAA27192.1.
V00620 Genomic DNA. Translation: CAA23895.1.
PIRiS12637.
RefSeqiNP_065320.1. NC_002525.1.
WP_001029679.1. NZ_LRXU01000049.1.

Genome annotation databases

GeneIDi1238710.
KEGGipg:1238710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17693 Genomic DNA. Translation: CAA35683.1.
M37391 Genomic DNA. Translation: AAA24680.1.
AP002527 Genomic DNA. Translation: BAB12613.1.
X03474 Genomic DNA. Translation: CAA27192.1.
V00620 Genomic DNA. Translation: CAA23895.1.
PIRiS12637.
RefSeqiNP_065320.1. NC_002525.1.
WP_001029679.1. NZ_LRXU01000049.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F1ZX-ray2.40A/B1-273[»]
1T0FX-ray1.85A/B1-273[»]
ProteinModelPortaliP13988.
SMRiP13988. Positions 5-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13988. 1 interaction.

Proteomic databases

PRIDEiP13988.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1238710.
KEGGipg:1238710.

Miscellaneous databases

EvolutionaryTraceiP13988.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.1350.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR014832. TnsA_C.
IPR014833. TnsA_N.
IPR011856. tRNA_endonuc-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08721. Tn7_Tnp_TnsA_C. 1 hit.
PF08722. Tn7_Tnp_TnsA_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition."
    Flores C., Qadri M.I., Lichtenstein C.
    Nucleic Acids Res. 18:901-911(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of transposition proteins encoded by the bacterial transposon Tn7."
    Orle K.A., Craig N.L.
    Gene 96:1-7(1990)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Organization and diversification of plasmid genomes: complete nucleotide sequence of the R721 genome."
    Sampei G., Motomura K., Masuda S., Yamaguchi T., Ando K., Oishi T., Furuya N., Komano T., Mizobuchi K.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: R721
  4. "Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator."
    Gay N.J., Tybulewicz V.L.J., Walker J.E.
    Biochem. J. 234:111-117(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-134.
  5. "The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products."
    Sarnovsky R.J., May E.W., Craig N.L.
    EMBO J. 15:6348-6361(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-28; THR-34; GLU-37; HIS-57; SER-60; ASP-108; ASP-114; GLU-144; GLU-147 AND GLU-149, COFACTOR.
  6. "A minimal system for Tn7 transposition: the transposon-encoded proteins TnsA and TnsB can execute DNA breakage and joining reactions that generate circularized Tn7 species."
    Biery M.C., Lopata M., Craig N.L.
    J. Mol. Biol. 297:25-37(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTNSA_ECOLX
AccessioniPrimary (citable) accession number: P13988
Secondary accession number(s): Q47662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.