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Protein

Transposon Tn7 transposition protein TnsA

Gene

tnsA

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and TnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas TnsABC and TnsD promote low-frequency insertion into many different sites.2 Publications

Cofactori

a divalent metal cation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi90 – 108H-T-H motifBy similarityAdd BLAST19

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • nuclease activity Source: InterPro
  • transposase activity Source: UniProtKB

GO - Biological processi

  • transposition, DNA-mediated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA recombination, Transposition

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Tn7 transposition protein TnsA
Gene namesi
Name:tnsA
Encoded oniPlasmid R7211 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic chromosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28D → A: No effect on recombination. 1 Publication1
Mutagenesisi34T → A: No effect on recombination. 1 Publication1
Mutagenesisi37E → A: Modest decrease in 5'-cleavage. 1 Publication1
Mutagenesisi57H → A: Prevents recombination, may effect protein structure. 1 Publication1
Mutagenesisi60S → A: Prevents recombination, may effect protein structure. 1 Publication1
Mutagenesisi108D → A: No effect on recombination. 1 Publication1
Mutagenesisi114D → A: Prevents 5'-cleavage. 1 Publication1
Mutagenesisi144E → A: No effect on recombination. 1 Publication1
Mutagenesisi147E → A: No effect on recombination. 1 Publication1
Mutagenesisi149E → A: Decrease in 5' cleavage. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726111 – 273Transposon Tn7 transposition protein TnsAAdd BLAST273

Interactioni

Protein-protein interaction databases

IntActiP13988. 1 interactor.

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 18Combined sources9
Turni19 – 22Combined sources4
Helixi26 – 28Combined sources3
Helixi35 – 37Combined sources3
Beta strandi45 – 48Combined sources4
Turni50 – 52Combined sources3
Beta strandi53 – 58Combined sources6
Helixi61 – 72Combined sources12
Beta strandi76 – 84Combined sources9
Helixi87 – 97Combined sources11
Beta strandi112 – 123Combined sources12
Beta strandi125 – 131Combined sources7
Helixi134 – 138Combined sources5
Helixi140 – 156Combined sources17
Beta strandi160 – 163Combined sources4
Helixi165 – 167Combined sources3
Helixi170 – 180Combined sources11
Helixi189 – 192Combined sources4
Helixi195 – 204Combined sources10
Turni205 – 207Combined sources3
Helixi210 – 220Combined sources11
Helixi227 – 237Combined sources11
Beta strandi240 – 242Combined sources3
Helixi249 – 251Combined sources3
Helixi254 – 256Combined sources3
Beta strandi262 – 264Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F1ZX-ray2.40A/B1-273[»]
1T0FX-ray1.85A/B1-273[»]
ProteinModelPortaliP13988.
SMRiP13988.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13988.

Family & Domainsi

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.1350.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR014832. TnsA_C.
IPR014833. TnsA_N.
IPR011856. tRNA_endonuc-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08721. Tn7_Tnp_TnsA_C. 1 hit.
PF08722. Tn7_Tnp_TnsA_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P13988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKANSSFSE VQIARRIKEG RGQGHGKDYI PWLTVQEVPS SGRSHRIYSH
60 70 80 90 100
KTGRVHHLLS DLELAVFLSL EWESSVLDIR EQFPLLPSDT RQIAIDSGIK
110 120 130 140 150
HPVIRGVDQV MSTDFLVDCK DGPFEQFAIQ VKPAAALQDE RTLEKLELER
160 170 180 190 200
RYWQQKQIPW FIFTDKEINP VVKENIEWLY SVKTEEVSAE LLAQLSPLAH
210 220 230 240 250
ILQEKGDENI INVCKQVDIA YDLELGKTLS EIRALTANGF IKFNIYKSFR
260 270
ANKCADLCIS QVVNMEELRY VAN
Length:273
Mass (Da):31,257
Last modified:January 1, 1990 - v1
Checksum:i2C2EFDE9A7D9C477
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139D → G in AAA24680 (Ref. 2) Curated1
Sequence conflicti227K → Q in AAA24680 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17693 Genomic DNA. Translation: CAA35683.1.
M37391 Genomic DNA. Translation: AAA24680.1.
AP002527 Genomic DNA. Translation: BAB12613.1.
X03474 Genomic DNA. Translation: CAA27192.1.
V00620 Genomic DNA. Translation: CAA23895.1.
PIRiS12637.
RefSeqiNP_065320.1. NC_002525.1.
WP_001029679.1. NZ_LXIU01000334.1.

Genome annotation databases

GeneIDi1238710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17693 Genomic DNA. Translation: CAA35683.1.
M37391 Genomic DNA. Translation: AAA24680.1.
AP002527 Genomic DNA. Translation: BAB12613.1.
X03474 Genomic DNA. Translation: CAA27192.1.
V00620 Genomic DNA. Translation: CAA23895.1.
PIRiS12637.
RefSeqiNP_065320.1. NC_002525.1.
WP_001029679.1. NZ_LXIU01000334.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F1ZX-ray2.40A/B1-273[»]
1T0FX-ray1.85A/B1-273[»]
ProteinModelPortaliP13988.
SMRiP13988.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13988. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1238710.

Miscellaneous databases

EvolutionaryTraceiP13988.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.1350.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR014832. TnsA_C.
IPR014833. TnsA_N.
IPR011856. tRNA_endonuc-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08721. Tn7_Tnp_TnsA_C. 1 hit.
PF08722. Tn7_Tnp_TnsA_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTNSA_ECOLX
AccessioniPrimary (citable) accession number: P13988
Secondary accession number(s): Q47662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.