UniProtKB - P13984 (T2FB_HUMAN)
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Protein
General transcription factor IIF subunit 2
Gene
GTF2F2
Organism
Homo sapiens (Human)
Status
Functioni
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.1 Publication
Catalytic activityi
ATP + H2O = ADP + phosphate.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- helicase activity Source: UniProtKB-KW
- transcription factor activity, core RNA polymerase II binding Source: GO_Central
GO - Biological processi
- 7-methylguanosine mRNA capping Source: Reactome
- fibroblast growth factor receptor signaling pathway Source: Reactome
- mRNA splicing, via spliceosome Source: Reactome
- positive regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
- positive regulation of transcription initiation from RNA polymerase II promoter Source: GO_Central
- positive regulation of viral transcription Source: Reactome
- RNA metabolic process Source: Reactome
- snRNA transcription by RNA polymerase II Source: Reactome
- transcription by RNA polymerase II Source: Reactome
- transcription elongation from RNA polymerase II promoter Source: Reactome
- transcription initiation from RNA polymerase II promoter Source: Reactome
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-HSA-112382. Formation of RNA Pol II elongation complex. R-HSA-113418. Formation of the Early Elongation Complex. R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat. R-HSA-167158. Formation of the HIV-1 Early Elongation Complex. R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE during HIV infection. R-HSA-167161. HIV Transcription Initiation. R-HSA-167162. RNA Polymerase II HIV Promoter Escape. R-HSA-167172. Transcription of the HIV genome. R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat. R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation. R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat. R-HSA-167243. Tat-mediated HIV elongation arrest and recovery. R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript. R-HSA-167287. HIV elongation arrest and recovery. R-HSA-167290. Pausing and recovery of HIV elongation. R-HSA-168325. Viral Messenger RNA Synthesis. R-HSA-674695. RNA Polymerase II Pre-transcription Events. R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes. R-HSA-6803529. FGFR2 alternative splicing. R-HSA-6807505. RNA polymerase II transcribes snRNA genes. R-HSA-72086. mRNA Capping. R-HSA-72163. mRNA Splicing - Major Pathway. R-HSA-72165. mRNA Splicing - Minor Pathway. R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA. R-HSA-73776. RNA Polymerase II Promoter Escape. R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening. R-HSA-75953. RNA Polymerase II Transcription Initiation. R-HSA-75955. RNA Polymerase II Transcription Elongation. R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance. R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE. R-HSA-8851708. Signaling by FGFR2 IIIa TM. |
Names & Taxonomyi
Protein namesi | Recommended name: General transcription factor IIF subunit 2 (EC:3.6.4.12)Alternative name(s): ATP-dependent helicase GTF2F2 General transcription factor IIF 30 kDa subunit Transcription initiation factor IIF subunit beta Short name: TFIIF-beta Transcription initiation factor RAP30 |
Gene namesi | Name:GTF2F2 Synonyms:RAP30 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000188342.11. |
HGNCi | HGNC:4653. GTF2F2. |
MIMi | 189969. gene. |
neXtProti | NX_P13984. |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 2963. |
OpenTargetsi | ENSG00000188342. |
PharmGKBi | PA29039. |
Polymorphism and mutation databases
BioMutai | GTF2F2. |
DMDMi | 464519. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000211235 | 2 – 249 | General transcription factor IIF subunit 2Add BLAST | 248 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 22 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 33 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 137 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 142 | PhosphoserineCombined sources | 1 | |
Modified residuei | 248 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P13984. |
MaxQBi | P13984. |
PaxDbi | P13984. |
PeptideAtlasi | P13984. |
PRIDEi | P13984. |
PTM databases
iPTMneti | P13984. |
PhosphoSitePlusi | P13984. |
Expressioni
Gene expression databases
Bgeei | ENSG00000188342. |
CleanExi | HS_GTF2F2. |
ExpressionAtlasi | P13984. baseline and differential. |
Genevisiblei | P13984. HS. |
Organism-specific databases
HPAi | HPA006912. |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1.By similarity2 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 109218. 51 interactors. |
CORUMi | P13984. |
DIPi | DIP-41680N. |
IntActi | P13984. 41 interactors. |
MINTi | P13984. |
STRINGi | 9606.ENSP00000340823. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 6 – 8 | Combined sources | 3 | |
Helixi | 10 – 13 | Combined sources | 4 | |
Beta strandi | 17 – 24 | Combined sources | 8 | |
Helixi | 25 – 31 | Combined sources | 7 | |
Beta strandi | 39 – 48 | Combined sources | 10 | |
Beta strandi | 51 – 58 | Combined sources | 8 | |
Helixi | 60 – 63 | Combined sources | 4 | |
Beta strandi | 80 – 86 | Combined sources | 7 | |
Beta strandi | 91 – 99 | Combined sources | 9 | |
Beta strandi | 104 – 116 | Combined sources | 13 | |
Helixi | 177 – 193 | Combined sources | 17 | |
Helixi | 199 – 205 | Combined sources | 7 | |
Helixi | 210 – 220 | Combined sources | 11 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BBY | NMR | - | A | 175-243 | [»] | |
1F3U | X-ray | 1.70 | A/C/E/G | 2-119 | [»] | |
2BBY | NMR | - | A | 175-243 | [»] | |
5IY6 | electron microscopy | 7.20 | T | 1-249 | [»] | |
5IY7 | electron microscopy | 8.60 | T | 1-249 | [»] | |
5IY8 | electron microscopy | 7.90 | T | 1-249 | [»] | |
5IY9 | electron microscopy | 6.30 | T | 1-249 | [»] | |
5IYA | electron microscopy | 5.40 | T | 1-249 | [»] | |
5IYB | electron microscopy | 3.90 | T | 1-249 | [»] | |
5IYC | electron microscopy | 3.90 | T | 1-249 | [»] | |
5IYD | electron microscopy | 3.90 | T | 1-249 | [»] | |
ProteinModelPortali | P13984. | |||||
SMRi | P13984. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P13984. |
Family & Domainsi
Sequence similaritiesi
Belongs to the TFIIF beta subunit family.Curated
Phylogenomic databases
eggNOGi | KOG2905. Eukaryota. COG5090. LUCA. |
GeneTreei | ENSGT00390000016051. |
HOGENOMi | HOG000294179. |
HOVERGENi | HBG001606. |
InParanoidi | P13984. |
KOi | K03139. |
OMAi | NMWELKK. |
OrthoDBi | EOG091G0IN2. |
PhylomeDBi | P13984. |
TreeFami | TF314290. |
Family and domain databases
Gene3Di | 1.10.10.10. 1 hit. |
InterProi | View protein in InterPro IPR003196. TFIIF_beta. IPR011039. TFIIF_interaction. IPR036388. WH-like_DNA-bd_sf. IPR036390. WH_DNA-bd_sf. |
PANTHERi | PTHR10445. PTHR10445. 1 hit. |
Pfami | View protein in Pfam PF02270. TFIIF_beta. 1 hit. |
PIRSFi | PIRSF015849. TFIIF-beta. 1 hit. |
SUPFAMi | SSF46785. SSF46785. 1 hit. SSF50916. SSF50916. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P13984-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG
60 70 80 90 100
RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS
110 120 130 140 150
SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVR LSQQLDKVVT
160 170 180 190 200
TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK
210 220 230 240
DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16901 mRNA. Translation: CAA34775.1. X59745 mRNA. Translation: CAA42419.1. AK291545 mRNA. Translation: BAF84234.1. BT019525 mRNA. Translation: AAV38332.1. AL138963 Genomic DNA. No translation available. AL138693 Genomic DNA. No translation available. CH471075 Genomic DNA. Translation: EAX08728.1. BC001771 mRNA. Translation: AAH01771.1. |
CCDSi | CCDS9395.1. |
PIRi | S06141. S18677. |
RefSeqi | NP_004119.1. NM_004128.2. |
UniGenei | Hs.654582. |
Genome annotation databases
Ensembli | ENST00000340473; ENSP00000340823; ENSG00000188342. |
GeneIDi | 2963. |
KEGGi | hsa:2963. |
UCSCi | uc001uzw.4. human. |
Similar proteinsi
Entry informationi
Entry namei | T2FB_HUMAN | |
Accessioni | P13984Primary (citable) accession number: P13984 Secondary accession number(s): A6NNS5, Q5W0H3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | February 1, 1994 | |
Last modified: | March 28, 2018 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |