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UniProtKB - P13984 (T2FB_HUMAN)

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Protein

General transcription factor IIF subunit 2

Gene

GTF2F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPSequence analysis8

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE during HIV infection.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-6803529. FGFR2 alternative splicing.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-8851708. Signaling by FGFR2 IIIa TM.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIF subunit 2 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase GTF2F2
General transcription factor IIF 30 kDa subunit
Transcription initiation factor IIF subunit beta
Short name:
TFIIF-beta
Transcription initiation factor RAP30
Gene namesi
Name:GTF2F2
Synonyms:RAP30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:4653. GTF2F2.

Subcellular locationi

GO - Cellular componenti

  • microtubule cytoskeleton Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • transcriptional preinitiation complex Source: CAFA
  • transcription factor TFIIF complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2963.
OpenTargetsiENSG00000188342.
PharmGKBiPA29039.

Polymorphism and mutation databases

BioMutaiGTF2F2.
DMDMi464519.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002112352 – 249General transcription factor IIF subunit 2Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei22N6-acetyllysineCombined sources1
Modified residuei33N6-acetyllysineCombined sources1
Modified residuei137N6-acetyllysineCombined sources1
Modified residuei142PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP13984.
MaxQBiP13984.
PaxDbiP13984.
PeptideAtlasiP13984.
PRIDEiP13984.

PTM databases

iPTMnetiP13984.
PhosphoSitePlusiP13984.

Expressioni

Gene expression databases

BgeeiENSG00000188342.
CleanExiHS_GTF2F2.
ExpressionAtlasiP13984. baseline and differential.
GenevisibleiP13984. HS.

Organism-specific databases

HPAiHPA006912.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1.By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi109218. 51 interactors.
DIPiDIP-41680N.
IntActiP13984. 38 interactors.
MINTiMINT-193667.
STRINGi9606.ENSP00000340823.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Helixi10 – 13Combined sources4
Beta strandi17 – 24Combined sources8
Helixi25 – 31Combined sources7
Beta strandi39 – 48Combined sources10
Beta strandi51 – 58Combined sources8
Helixi60 – 63Combined sources4
Beta strandi80 – 86Combined sources7
Beta strandi91 – 99Combined sources9
Beta strandi104 – 116Combined sources13
Helixi177 – 193Combined sources17
Helixi199 – 205Combined sources7
Helixi210 – 220Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BBYNMR-A175-243[»]
1F3UX-ray1.70A/C/E/G2-119[»]
2BBYNMR-A175-243[»]
5IY6electron microscopy7.20T1-249[»]
5IY7electron microscopy8.60T1-249[»]
5IY8electron microscopy7.90T1-249[»]
5IY9electron microscopy6.30T1-249[»]
5IYAelectron microscopy5.40T1-249[»]
5IYBelectron microscopy3.90T1-249[»]
5IYCelectron microscopy3.90T1-249[»]
5IYDelectron microscopy3.90T1-249[»]
ProteinModelPortaliP13984.
SMRiP13984.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13984.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIF beta subunit family.Curated

Phylogenomic databases

eggNOGiKOG2905. Eukaryota.
COG5090. LUCA.
GeneTreeiENSGT00390000016051.
HOGENOMiHOG000294179.
HOVERGENiHBG001606.
InParanoidiP13984.
KOiK03139.
OMAiNMWELKK.
OrthoDBiEOG091G0IN2.
PhylomeDBiP13984.
TreeFamiTF314290.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR10445. PTHR10445. 1 hit.
PfamiView protein in Pfam
PF02270. TFIIF_beta. 1 hit.
PIRSFiPIRSF015849. TFIIF-beta. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50916. SSF50916. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG 
        60         70         80         90        100
RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS 
       110        120        130        140        150
SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVR LSQQLDKVVT 
       160        170        180        190        200
TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK 
       210        220        230        240 
DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD
Length:249
Mass (Da):28,380
Last modified:February 1, 1994 - v2
Checksum:i05A1A9F8D31B749C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16901 mRNA. Translation: CAA34775.1.
X59745 mRNA. Translation: CAA42419.1.
AK291545 mRNA. Translation: BAF84234.1.
BT019525 mRNA. Translation: AAV38332.1.
AL138963, AL138693 Genomic DNA. Translation: CAH72032.1.
AL138693, AL138963 Genomic DNA. Translation: CAH72459.1.
CH471075 Genomic DNA. Translation: EAX08728.1.
BC001771 mRNA. Translation: AAH01771.1.
CCDSiCCDS9395.1.
PIRiS06141.
S18677.
RefSeqiNP_004119.1. NM_004128.2.
UniGeneiHs.654582.

Genome annotation databases

EnsembliENST00000340473; ENSP00000340823; ENSG00000188342.
GeneIDi2963.
KEGGihsa:2963.
UCSCiuc001uzw.4. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2FB_HUMAN
AccessioniPrimary (citable) accession number: P13984
Secondary accession number(s): A6NNS5, Q5W0H3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1994
Last modified: July 5, 2017
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families