Reviewed, UniProtKB/Swiss-Prot P13978 (ERMC3_STAAU)

Names and origin

Protein names

Recommended name:
    rRNA adenine N-6-methyltransferase
    EC=2.1.1.48
Alternative name(s):
    Macrolide-lincosamide-streptogramin B resistance protein
    Erythromycin resistance protein

Gene names

Name:

ermC

Encoded on

Plasmid pT48

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	rRNA adenine N-6-methyltransferase		PRO_0000101684
Sites
	Binding site	11	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	13	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	38	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	84	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13978-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 765C245C40DAD6C0	FASTA	244	28,873
	10 20 30 40 50 60 MNEKNIKHSQ NFITSKHNID KIMTNIRLNE HDNIFEIGSG KGHFTLELVQ RCNFVTAIEI 70 80 90 100 110 120 DHKLCKTTEN KLVDHDNFQV LNKDILQFKF PKNQSYKIFG NIPYNISTDI IRKIVFDSIA 130 140 150 160 170 180 DEIYLIVEYG FAKRLLNTKR SLALLLMAEV DISILSMVPR EYFHPKPKVN SSLIRLNRKK 190 200 210 220 230 240 SRISHKDKQK YNYFVMKWVN KEYKKIFTKN QFNNSLKHAG IDDLNNISFE QFLSLFNSYK LFNK

References

[1]

"The nucleotide sequence of Staphylococcus aureus plasmid pT48 conferring inducible macrolide-lincosamide-streptogramin B resistance and comparison with similar plasmids expressing constitutive resistance." Catchpole I., Thomas C., Davies A., Dyke K.G.H. J. Gen. Microbiol. 134:697-709(1988) [PubMed: 3141573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19652 Unassigned DNA. Translation: AAA20192.1.
PIR	B46568.
3D structure databases
HSSP	HSSP built from PDB template 1QAN based on UniProtKB P13956.
SMR	P13978. Positions 10-244.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.48. 95.
Family and domain databases
InterPro	IPR001737. RRNA_Ade_Mease_Trfase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERMC3_STAAU

Accession

Primary (citable) accession number: P13978

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	March 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)