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Protein

Dihydrofolate reductase type 1 from Tn4003

Gene

dfrA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dhfrXII), Dihydrofolate reductase (dfrK), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase type 1 from Tn4003 (dfrA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrK), Dihydrofolate reductase (RK97_06330), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrK), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrK), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrA), Dihydrofolate reductase (dfrG)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28Substrate1 Publication1
Binding sitei58Substrate1 Publication1
Binding sitei112Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 8NADP1 Publication2
Nucleotide bindingi15 – 20NADP1 Publication6
Nucleotide bindingi44 – 47NADP1 Publication4
Nucleotide bindingi63 – 66NADP1 Publication4
Nucleotide bindingi93 – 98NADP1 Publication6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.5.1.3. 3352.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type 1 from Tn4003 (EC:1.5.1.3)
Gene namesi
Name:dfrA
Encoded oniPlasmid pSK10 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44A → G: Increases trimethoprim sensitivity; when associated with F-99. 1 Publication1
Mutagenesisi99Y → F: Increases trimethoprim sensitivity; when associated with G-44. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001864052 – 161Dihydrofolate reductase type 1 from Tn4003Add BLAST160

Proteomic databases

PRIDEiP13955.

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi14 – 17Combined sources4
Helixi26 – 36Combined sources11
Beta strandi39 – 44Combined sources6
Helixi45 – 51Combined sources7
Beta strandi58 – 63Combined sources6
Beta strandi75 – 77Combined sources3
Helixi81 – 84Combined sources4
Beta strandi90 – 94Combined sources5
Helixi96 – 102Combined sources7
Turni103 – 105Combined sources3
Beta strandi107 – 116Combined sources10
Beta strandi120 – 123Combined sources4
Turni129 – 131Combined sources3
Beta strandi132 – 139Combined sources8
Beta strandi143 – 145Combined sources3
Beta strandi150 – 157Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W9SX-ray1.80A/B/C/D/E/F1-161[»]
2W9TX-ray2.35A/B1-161[»]
ProteinModelPortaliP13955.
SMRiP13955.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13955.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 157DHFRPROSITE-ProRule annotationAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 8Substrate binding1 Publication3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSIIVAHD KQRVIGYQNQ LPWHLPNDLK HIKQLTTGNT LVMARKTFNS
60 70 80 90 100
IGKPLPNRRN VVLTNQASFH HEGVDVINSL DEIKELSGHV FIFGGQTLYE
110 120 130 140 150
AMIDQVDDMY ITVIDGKFQG DTFFPPYTFE NWEVESSVEG QLDEKNTIPH
160
TFLHLVRRKG K
Length:161
Mass (Da):18,461
Last modified:January 23, 2007 - v3
Checksum:iF829D6109A3AF3A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13290 Genomic DNA. Translation: CAA31649.1.
Y07536 Genomic DNA. Translation: CAA68824.1.
PIRiS04164.
RefSeqiNP_877983.1. NC_005054.1.
WP_000175735.1. NZ_LWBY01000050.1.
YP_002790943.1. NC_012547.1.
YP_003813115.1. NC_014369.1.
YP_006937652.1. NC_013320.1.
YP_006937707.1. NC_013321.1.
YP_006938354.1. NC_013338.1.
YP_006938562.1. NC_013343.1.

Genome annotation databases

GeneIDi13874802.
13874858.
13875495.
13875735.
2598376.
7751508.
9487075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13290 Genomic DNA. Translation: CAA31649.1.
Y07536 Genomic DNA. Translation: CAA68824.1.
PIRiS04164.
RefSeqiNP_877983.1. NC_005054.1.
WP_000175735.1. NZ_LWBY01000050.1.
YP_002790943.1. NC_012547.1.
YP_003813115.1. NC_014369.1.
YP_006937652.1. NC_013320.1.
YP_006937707.1. NC_013321.1.
YP_006938354.1. NC_013338.1.
YP_006938562.1. NC_013343.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W9SX-ray1.80A/B/C/D/E/F1-161[»]
2W9TX-ray2.35A/B1-161[»]
ProteinModelPortaliP13955.
SMRiP13955.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP13955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13874802.
13874858.
13875495.
13875735.
2598376.
7751508.
9487075.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.
BRENDAi1.5.1.3. 3352.

Miscellaneous databases

EvolutionaryTraceiP13955.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYRA_STAAU
AccessioniPrimary (citable) accession number: P13955
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two DhfR isozymes in S.aureus, this one is plasmid-encoded and is resistant to trimethoprim.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.