Beta-3 adrenergic receptor - Homo sapiens (Human)

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P13945 (ADRB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 134. History...

Clusters with 100%, 90%, 50% identity |

Documents (7) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Beta-3 adrenergic receptor

Alternative name(s):
Beta-3 adrenoreceptor
Short name=Beta-3 adrenoceptor

Gene names

Name:	ADRB3
Synonyms:	ADRB3R, B3AR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed mainly in adipose tissues.
Polymorphism	The variant Arg-64 seems to be associated with weight gain (obesity) and to is also associated with susceptibility to non-insulin-dependent diabetes mellitus (NIDDM).
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Disease	Diabetes mellitus Obesity
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Traceable author statement. Source: ProtInc energy reserve metabolic process Traceable author statement. Source: ProtInc positive regulation of MAPKKK cascade Inferred from direct assay. Source: HGNC
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc receptor complex Inferred from direct assay. Source: HGNC
Molecular function	beta3-adrenergic receptor activity Inferred from mutant phenotype. Source: HGNC protein homodimerization activity Inferred from direct assay. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 408

408

Beta-3 adrenergic receptor

PRO_0000069143

Regions

Topological domain

1 – 36

Extracellular By similarity

Transmembrane

37 – 63

Helical; Name=1; By similarity

Topological domain

64 – 72

Cytoplasmic By similarity

Transmembrane

73 – 91

Helical; Name=2; By similarity

Topological domain

92 – 111

Extracellular By similarity

Transmembrane

112 – 133

Helical; Name=3; By similarity

Topological domain

134 – 155

Cytoplasmic By similarity

Transmembrane

156 – 178

Helical; Name=4; By similarity

Topological domain

179 – 203

Extracellular By similarity

Transmembrane

204 – 225

Helical; Name=5; By similarity

Topological domain

226 – 292

Cytoplasmic By similarity

Transmembrane

293 – 314

Helical; Name=6; By similarity

Topological domain

315 – 326

Extracellular By similarity

Transmembrane

327 – 347

Helical; Name=7; By similarity

Topological domain

348 – 408

Cytoplasmic By similarity

Region

198 – 212

Agonist and antagonist binding By similarity

Region

305 – 312

Agonist and antagonist binding By similarity

Region

332 – 336

Agonist and antagonist binding By similarity

Sites

Binding site

117

Agonist or antagonist By similarity

Binding site

122

Agonist or antagonist By similarity

Amino acid modifications

Lipidation

361

S-palmitoyl cysteine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

110 ↔ 196

By similarity

Disulfide bond

189 ↔ 195

By similarity

Natural variations

Natural variant

W → R. Ref.5 Ref.8 Ref.9 Ref.10 Ref.11
Corresponds to variant rs4994 [ dbSNP | Ensembl ].

VAR_003456

Natural variant

249

E → K.
Corresponds to variant rs28364012 [ dbSNP | Ensembl ].

VAR_029205

Natural variant

265

T → M. Ref.11
Corresponds to variant rs4995 [ dbSNP | Ensembl ].

VAR_014166

Natural variant

353

R → C. Ref.5
Corresponds to variant rs36031925 [ dbSNP | Ensembl ].

VAR_025102

Sequences

Sequence

Length

Mass (Da)

Tools

P13945 [UniParc].

Last modified February 1, 1994. Version 3.
Checksum: E98BD6C130DD977B
Show »

FASTA

408

43,519

        10         20         30         40         50         60 
MAPWPHENSS LAPWPDLPTL APNTANTSGL PGVPWEAALA GALLALAVLA TVGGNLLVIV 

        70         80         90        100        110        120 
AIAWTPRLQT MTNVFVTSLA AADLVMGLLV VPPAATLALT GHWPLGATGC ELWTSVDVLC 

       130        140        150        160        170        180 
VTASIETLCA LAVDRYLAVT NPLRYGALVT KRCARTAVVL VWVVSAAVSF APIMSQWWRV 

       190        200        210        220        230        240 
GADAEAQRCH SNPRCCAFAS NMPYVLLSSS VSFYLPLLVM LFVYARVFVV ATRQLRLLRG 

       250        260        270        280        290        300 
ELGRFPPEES PPAPSRSLAP APVGTCAPPE GVPACGRRPA RLLPLREHRA LCTLGLIMGT 

       310        320        330        340        350        360 
FTLCWLPFFL ANVLRALGGP SLVPGPAFLA LNWLGYANSA FNPLIYCRSP DFRSAFRRLL 

       370        380        390        400 
CRCGRRLPPE PCAAARPALF PSGVPAARSS PAQPRLCQRL DGASWGVS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the human beta 3-adrenergic receptor." Emorine L.J., Marullo S., Briend-Sutren M.-M., Patey G., Tate K., Delavier-Klutchko C., Strosberg A.D. Science 245:1118-1121(1989) [PubMed: 2570461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The promoter and intron/exon structure of the human and mouse beta 3-adrenergic-receptor genes." van Spronsen A., Nahmias C., Krief S., Briend-Sutren M.-M., Strosberg A.D., Emorine L.J. Eur. J. Biochem. 213:1117-1124(1993) [PubMed: 8389293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
[3]	"Molecular cloning of a human beta 3-adrenergic receptor cDNA." Lelias J.M., Kaghad M., Rodriguez M., Chalon P., Bonnin J., Dupre I., Delpech B., Bensaid M., Lefur G., Ferrara P., Caput D. FEBS Lett. 324:127-130(1993) [PubMed: 8389717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	SeattleSNPs variation discovery resource Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-64 AND CYS-353.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Rodent and human beta 3-adrenergic receptor genes contain an intron within the protein-coding block." Granneman J.G., Lahners K.N., Rao D.D. Mol. Pharmacol. 42:964-970(1992) [PubMed: 1336117] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 392-408.
[8]	"Genetic variation in the beta 3-adrenergic receptor and an increased capacity to gain weight in patients with morbid obesity." Clement K., Vaisse C., Manning B.S.J., Basdevant A., Guy-Grand B., Ruiz J., Silver K.D., Shuldiner A.R., Froguel P., Strosberg A.D. N. Engl. J. Med. 333:352-354(1995) [PubMed: 7609752] [Abstract] Cited for: VARIANT ARG-64.
[9]	"Association of Trp64Arg mutation of the beta3-adrenergic-receptor with NIDDM and body weight gain." Fujisawa T., Ikegami H., Yamato E., Takekawa K., Nakagawa Y., Hamada Y., Oga T., Ueda H., Shintani M., Fukuda M., Ogihara T. Diabetologia 39:349-352(1996) [PubMed: 8721782] [Abstract] Cited for: VARIANT ARG-64.
[10]	"Pharmacological characterization of a recently described human beta 3-adrenergic receptor mutant." Candelore M.R., Deng L., Tota L.M., Kelly L.J., Cascieri M.A., Strader C.D. Endocrinology 137:2638-2641(1996) [PubMed: 8641219] [Abstract] Cited for: VARIANT ARG-64.
[11]	"Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis." Halushka M.K., Fan J.-B., Bentley K., Hsie L., Shen N., Weder A., Cooper R., Lipshutz R., Chakravarti A. Nat. Genet. 22:239-247(1999) [PubMed: 10391210] [Abstract] Cited for: VARIANTS ARG-64 AND MET-265.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M29932 Genomic DNA. Translation: AAA35550.1. Different termination.
X72861 Genomic DNA. Translation: CAA51383.1.
X70811 mRNA. Translation: CAA50141.1.
X70812 Genomic DNA. Translation: CAA50142.1. Sequence problems.
X70812 Genomic DNA. Translation: CAA50143.1. Sequence problems.
AY487247 mRNA. Translation: AAR37414.1.
DQ104441 Genomic DNA. Translation: AAY88743.1.
BC075017 mRNA. Translation: AAH75017.1.
S53291 mRNA. Translation: AAB24837.1.

IPI

IPI00011286.

PIR

I57941.
QRHUBE. S33751.

RefSeq

NP_000016.1. NM_000025.2.

UniGene

Hs.2549.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CDW	model	-	A	1-408	[»]

ProteinModelPortal

P13945.

SMR

P13945. Positions 32-361.

ModBase

Search...

Protein-protein interaction databases

STRING

P13945.

Protein family/group databases

GPCRDB

Search...

Polymorphism databases

DMDM

461604.

Proteomic databases

PRIDE

P13945.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000345060; ENSP00000343782; ENSG00000188778.

GeneID

155.

KEGG

hsa:155.

UCSC

uc003xkr.1. human.

Organism-specific databases

CTD

155.

GeneCards

GC08M037822.

H-InvDB

HIX0034321.

HGNC

HGNC:288. ADRB3.

MIM

109691. gene.

neXtProt

NX_P13945.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG11474.

GeneTree

ENSGT00590000082831.

HOGENOM

HBG445348.

HOVERGEN

HBG106962.

InParanoid

P13945.

OMA

TNPLRYG.

OrthoDB

EOG4NKBVZ.

PhylomeDB

P13945.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P13945.

Bgee

P13945.

CleanEx

HS_ADRB3.

Genevestigator

P13945.

GermOnline

ENSG00000188778. Homo sapiens.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR000681. Adrgc_rcpt_B3.
IPR002233. Adrnrgc_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]

K04143.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR01103. ADRENERGICR.
PR00563. ADRENRGCB3AR.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00368. Norepinephrine.
DB00960. Pindolol.
DB00571. Propranolol.

NextBio

617.

SOURCE

Search...

Entry information

Entry name

ADRB3_HUMAN

Accession

Primary (citable) accession number: P13945
Secondary accession number(s): Q4JFT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 134 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.