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P13933

- GUN1_STRSS

UniProt

P13933 - GUN1_STRSS

Protein

Endoglucanase 1

Gene

casA

Organism
Streptomyces sp. (strain KSM-9)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 3 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    CMCase I preferentially hydrolyzes carboxymethyl cellulose (CMC).

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    pH dependencei

    Optimum pH is 8.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei154 – 1541PROSITE-ProRule annotation
    Active sitei192 – 1921Proton donorPROSITE-ProRule annotation
    Active sitei339 – 3391NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH6. Glycoside Hydrolase Family 6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 1 (EC:3.2.1.4)
    Alternative name(s):
    CMCase I
    Carboxymethyl cellulase
    Cellulase
    Endo-1,4-beta-glucanase
    Gene namesi
    Name:casA
    OrganismiStreptomyces sp. (strain KSM-9)
    Taxonomic identifieri74575 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei? – 701 PublicationPRO_0000007907
    Signal peptidei1 – ?
    Chaini71 – 359289Endoglucanase 1PRO_0000007908Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi155 ↔ 199By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP13933.
    SMRiP13933. Positions 89-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.40. 1 hit.
    InterProiIPR016288. Beta_cellobiohydrolase.
    IPR001524. Glyco_hydro_6_CS.
    [Graphical view]
    PfamiPF01341. Glyco_hydro_6. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
    PRINTSiPR00733. GLHYDRLASE6.
    SUPFAMiSSF51989. SSF51989. 1 hit.
    PROSITEiPS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
    PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13933-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENPRTTPTP TPLRRRRSER RARGGRVLTA LTGVTLLAGL AIAPAATGAS    50
    PSPAPPASPA PSADSGTADA GTTALPSMEL YRAEAGVHAW LDANPGDHRA 100
    PLIAERIGSQ PQAVWFAGAY NPGTITQQVA EVTSAAAAAG QLPVVVPYMI 150
    PFRDCGNHSG GGAPSFAAYA EWSGLFAAGL GSEPVVVVLE PDAIPLIDCL 200
    DNQQRAERLA ALAGLAEAVT DANPEARVYY DVGHSAWHAP AAIAPTLVEA 250
    GILEHGAGIA TNISNYRTTT DETAYASAVI AELGGGLGAV VDTSRNGNGP 300
    LGSEWCDPPG RLVGNNPTVN PGVPGVDAFL WIKLPGELDG CDGPVGSFSP 350
    AKAYELAGG 359
    Length:359
    Mass (Da):36,515
    Last modified:June 1, 1994 - v3
    Checksum:iF1300238834E906C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03218 Genomic DNA. Translation: AAA26713.1.
    X61008 Genomic DNA. Translation: CAA43330.1.
    M20921 Genomic DNA. Translation: AAA26776.1. Sequence problems.
    PIRiJN0544.
    JT0308.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03218 Genomic DNA. Translation: AAA26713.1 .
    X61008 Genomic DNA. Translation: CAA43330.1 .
    M20921 Genomic DNA. Translation: AAA26776.1 . Sequence problems.
    PIRi JN0544.
    JT0308.

    3D structure databases

    ProteinModelPortali P13933.
    SMRi P13933. Positions 89-358.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH6. Glycoside Hydrolase Family 6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.40. 1 hit.
    InterProi IPR016288. Beta_cellobiohydrolase.
    IPR001524. Glyco_hydro_6_CS.
    [Graphical view ]
    Pfami PF01341. Glyco_hydro_6. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001100. Beta_cellobiohydrolase. 1 hit.
    PRINTSi PR00733. GLHYDRLASE6.
    SUPFAMi SSF51989. SSF51989. 1 hit.
    PROSITEi PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
    PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain."
      Nakai R., Horinouchi S., Beppu T.
      Gene 65:229-238(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 71-95.
    2. "Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of beta-1,4-glucanases."
      Damude H.G., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.
      Gene 123:105-107(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
    3. Cited for: PROTEIN SEQUENCE OF 185-222, SEQUENCE REVISION.

    Entry informationi

    Entry nameiGUN1_STRSS
    AccessioniPrimary (citable) accession number: P13933
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 82 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3