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Protein

Endoglucanase 1

Gene

casA

Organism
Streptomyces sp. (strain KSM-9)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CMCase I preferentially hydrolyzes carboxymethyl cellulose (CMC).

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 8.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541PROSITE-ProRule annotation
Active sitei192 – 1921Proton donorPROSITE-ProRule annotation
Active sitei339 – 3391NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH6. Glycoside Hydrolase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 1 (EC:3.2.1.4)
Alternative name(s):
CMCase I
Carboxymethyl cellulase
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:casA
OrganismiStreptomyces sp. (strain KSM-9)
Taxonomic identifieri74575 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 701 PublicationPRO_0000007907
Signal peptidei1 – ?
Chaini71 – 359289Endoglucanase 1PRO_0000007908Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi155 ↔ 199By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP13933.
SMRiP13933. Positions 89-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.40. 1 hit.
InterProiIPR016288. Beta_cellobiohydrolase.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
PfamiPF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSiPR00733. GLHYDRLASE6.
SUPFAMiSSF51989. SSF51989. 1 hit.
PROSITEiPS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENPRTTPTP TPLRRRRSER RARGGRVLTA LTGVTLLAGL AIAPAATGAS
60 70 80 90 100
PSPAPPASPA PSADSGTADA GTTALPSMEL YRAEAGVHAW LDANPGDHRA
110 120 130 140 150
PLIAERIGSQ PQAVWFAGAY NPGTITQQVA EVTSAAAAAG QLPVVVPYMI
160 170 180 190 200
PFRDCGNHSG GGAPSFAAYA EWSGLFAAGL GSEPVVVVLE PDAIPLIDCL
210 220 230 240 250
DNQQRAERLA ALAGLAEAVT DANPEARVYY DVGHSAWHAP AAIAPTLVEA
260 270 280 290 300
GILEHGAGIA TNISNYRTTT DETAYASAVI AELGGGLGAV VDTSRNGNGP
310 320 330 340 350
LGSEWCDPPG RLVGNNPTVN PGVPGVDAFL WIKLPGELDG CDGPVGSFSP

AKAYELAGG
Length:359
Mass (Da):36,515
Last modified:June 1, 1994 - v3
Checksum:iF1300238834E906C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03218 Genomic DNA. Translation: AAA26713.1.
X61008 Genomic DNA. Translation: CAA43330.1.
M20921 Genomic DNA. Translation: AAA26776.1. Sequence problems.
PIRiJN0544.
JT0308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03218 Genomic DNA. Translation: AAA26713.1.
X61008 Genomic DNA. Translation: CAA43330.1.
M20921 Genomic DNA. Translation: AAA26776.1. Sequence problems.
PIRiJN0544.
JT0308.

3D structure databases

ProteinModelPortaliP13933.
SMRiP13933. Positions 89-358.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH6. Glycoside Hydrolase Family 6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.40. 1 hit.
InterProiIPR016288. Beta_cellobiohydrolase.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
PfamiPF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSiPR00733. GLHYDRLASE6.
SUPFAMiSSF51989. SSF51989. 1 hit.
PROSITEiPS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain."
    Nakai R., Horinouchi S., Beppu T.
    Gene 65:229-238(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 71-95.
  2. "Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of beta-1,4-glucanases."
    Damude H.G., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.
    Gene 123:105-107(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
  3. Cited for: PROTEIN SEQUENCE OF 185-222, SEQUENCE REVISION.

Entry informationi

Entry nameiGUN1_STRSS
AccessioniPrimary (citable) accession number: P13933
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 1994
Last modified: June 24, 2015
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.