P13933 (GUN1_STRSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 1
EC=3.2.1.4

Alternative name(s):
CMCase I
Carboxymethyl cellulase
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:

casA

Organism

Streptomyces sp. (strain KSM-9)

Taxonomic identifier

74575 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CMCase I preferentially hydrolyzes carboxymethyl cellulose (CMC).
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 6 (cellulase B) family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.5.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?
	Propeptide	? – 70				PRO_0000007907
	Chain	71 – 359	289	Endoglucanase 1		PRO_0000007908
Sites
	Active site	154	1	By similarity
	Active site	192	1	Proton donor By similarity
	Active site	339	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	155 ↔ 199		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13933 [UniParc]. Last modified June 1, 1994. Version 3. Checksum: F1300238834E906C	FASTA	359	36,515
	10 20 30 40 50 60 MENPRTTPTP TPLRRRRSER RARGGRVLTA LTGVTLLAGL AIAPAATGAS PSPAPPASPA 70 80 90 100 110 120 PSADSGTADA GTTALPSMEL YRAEAGVHAW LDANPGDHRA PLIAERIGSQ PQAVWFAGAY 130 140 150 160 170 180 NPGTITQQVA EVTSAAAAAG QLPVVVPYMI PFRDCGNHSG GGAPSFAAYA EWSGLFAAGL 190 200 210 220 230 240 GSEPVVVVLE PDAIPLIDCL DNQQRAERLA ALAGLAEAVT DANPEARVYY DVGHSAWHAP 250 260 270 280 290 300 AAIAPTLVEA GILEHGAGIA TNISNYRTTT DETAYASAVI AELGGGLGAV VDTSRNGNGP 310 320 330 340 350 LGSEWCDPPG RLVGNNPTVN PGVPGVDAFL WIKLPGELDG CDGPVGSFSP AKAYELAGG

References

[1]	"Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain." Nakai R., Horinouchi S., Beppu T. Gene 65:229-238(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 71-95.
[2]	"Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of beta-1,4-glucanases." Damude H.G., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A. Gene 123:105-107(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
[3]	"Structural and functional relationships in two families of beta-1,4-glycanases." Gilkes N.R., Claeyssens M., Aebersold R., Henrissat B., Meinke A., Morrison H.D., Kilburn D.G., Warren R.A.J., Miller R.C. Jr. Eur. J. Biochem. 202:367-377(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 185-222, SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03218 Genomic DNA. Translation: AAA26713.1. X61008 Genomic DNA. Translation: CAA43330.1. M20921 Genomic DNA. Translation: AAA26776.1. Sequence problems.
PIR	JN0544. JT0308.
3D structure databases
ProteinModelPortal	P13933.
SMR	P13933. Positions 89-358.
ModBase	Search...
Protein family/group databases
CAZy	GH6. Glycoside Hydrolase Family 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.40. 1 hit.
InterPro	IPR016288. Beta_cellobiohydrolase. IPR001524. Glyco_hydro_6_CS. [Graphical view]
Pfam	PF01341. Glyco_hydro_6. 1 hit. [Graphical view]
PIRSF	PIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTS	PR00733. GLHYDRLASE6.
SUPFAM	SSF51989. Glyco_hydro_6. 1 hit.
PROSITE	PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit. PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_STRSS

Accession

Primary (citable) accession number: P13933

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program