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P13928

- ANXA8_HUMAN

UniProt

P13928 - ANXA8_HUMAN

Protein

Annexin A8

Gene

ANXA8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 3 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi266 – 2661Calcium; via carbonyl oxygen
    Metal bindingi268 – 2681Calcium; via carbonyl oxygen
    Metal bindingi270 – 2701Calcium; via carbonyl oxygen
    Metal bindingi310 – 3101Calcium

    GO - Molecular functioni

    1. actin filament binding Source: UniProt
    2. calcium-dependent phospholipid binding Source: UniProt
    3. calcium ion binding Source: UniProt
    4. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProt
    5. phosphatidylinositol-3,4-bisphosphate binding Source: UniProt
    6. phosphatidylinositol-4,5-bisphosphate binding Source: UniProt

    GO - Biological processi

    1. blood coagulation Source: UniProtKB-KW
    2. endosomal transport Source: UniProt
    3. endosome organization Source: UniProt
    4. negative regulation of phospholipase A2 activity Source: UniProt
    5. negative regulation of serine-type endopeptidase activity Source: UniProt

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Keywords - Ligandi

    Calcium, Calcium/phospholipid-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Annexin A8
    Alternative name(s):
    Annexin VIII
    Annexin-8
    Vascular anticoagulant-beta
    Short name:
    VAC-beta
    Gene namesi
    Name:ANXA8
    Synonyms:ANX8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:546. ANXA8.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProt
    2. late endosome membrane Source: UniProt
    3. plasma membrane Source: UniProt

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24836.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Annexin A8PRO_0000067503Add
    BLAST

    Proteomic databases

    MaxQBiP13928.
    PaxDbiP13928.
    PRIDEiP13928.

    PTM databases

    PhosphoSiteiP13928.

    Expressioni

    Gene expression databases

    BgeeiP13928.
    CleanExiHS_ANXA8.
    GenevestigatoriP13928.

    Interactioni

    Protein-protein interaction databases

    BioGridi106745. 7 interactions.
    575558. 3 interactions.
    IntActiP13928. 3 interactions.
    MINTiMINT-1892975.
    STRINGi9606.ENSP00000341674.

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi24 – 3411
    Beta strandi35 – 384
    Helixi41 – 488
    Helixi53 – 6715
    Helixi71 – 788
    Helixi81 – 9111
    Helixi96 – 10712
    Beta strandi108 – 1103
    Helixi113 – 12210
    Helixi125 – 13915
    Helixi143 – 1508
    Helixi153 – 16311
    Beta strandi167 – 1704
    Helixi176 – 19116
    Beta strandi193 – 1953
    Helixi198 – 20710
    Helixi210 – 22415
    Helixi228 – 2358
    Helixi238 – 25215
    Helixi254 – 26613
    Beta strandi267 – 2704
    Helixi273 – 28311
    Turni284 – 2863
    Helixi288 – 29912
    Helixi303 – 3108
    Helixi313 – 32311

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W3WX-ray1.99A1-327[»]
    1W45X-ray2.51A/B1-327[»]
    ProteinModelPortaliP13928.
    SMRiP13928. Positions 8-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13928.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati30 – 9061Annexin 1Add
    BLAST
    Repeati102 – 16261Annexin 2Add
    BLAST
    Repeati187 – 24761Annexin 3Add
    BLAST
    Repeati262 – 32261Annexin 4Add
    BLAST

    Domaini

    A pair of annexin repeats may form one binding site for calcium and phospholipid.

    Sequence similaritiesi

    Belongs to the annexin family.Curated
    Contains 4 annexin repeats.Curated

    Keywords - Domaini

    Annexin, Repeat

    Phylogenomic databases

    eggNOGiNOG267770.
    HOGENOMiHOG000158803.
    HOVERGENiHBG061815.
    InParanoidiP13928.
    KOiK17096.
    OMAiMAPHYFA.
    PhylomeDBiP13928.
    TreeFamiTF105452.

    Family and domain databases

    Gene3Di1.10.220.10. 4 hits.
    InterProiIPR001464. Annexin.
    IPR018502. Annexin_repeat.
    IPR018252. Annexin_repeat_CS.
    IPR009115. AnnexinVIII.
    [Graphical view]
    PfamiPF00191. Annexin. 4 hits.
    [Graphical view]
    PRINTSiPR00196. ANNEXIN.
    PR01808. ANNEXINVIII.
    SMARTiSM00335. ANX. 4 hits.
    [Graphical view]
    PROSITEiPS00223. ANNEXIN. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P13928-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAWWKSWIEQ EGVTVKSSSH FNPDPDAETL YKAMKGIGTN EQAIIDVLTK    50
    RSNTQRQQIA KSFKAQFGKD LTETLKSELS GKFERLIVAL MYPPYRYEAK 100
    ELHDAMKGLG TKEGVIIEIL ASRTKNQLRE IMKAYEEDYG SSLEEDIQAD 150
    TSGYLERILV CLLQGSRDDV SSFVDPGLAL QDAQDLYAAG EKIRGTDEMK 200
    FITILCTRSA THLLRVFEEY EKIANKSIED SIKSETHGSL EEAMLTVVKC 250
    TQNLHSYFAE RLYYAMKGAG TRDGTLIRNI VSRSEIDLNL IKCHFKKMYG 300
    KTLSSMIMED TSGDYKNALL SLVGSDP 327
    Length:327
    Mass (Da):36,881
    Last modified:November 25, 2008 - v3
    Checksum:i5DBBDBB6E723C298
    GO
    Isoform 2 (identifier: P13928-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         138-141: DYGS → GQQG
         142-327: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:141
    Mass (Da):16,070
    Checksum:iD7D3F13E09C28D19
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581Q → T in AAB46383. (PubMed:1313714)Curated
    Sequence conflicti83 – 831F → L in AAB46383. (PubMed:1313714)Curated
    Sequence conflicti157 – 1571R → S in AAB46383. (PubMed:1313714)Curated
    Sequence conflicti313 – 3142GD → RY in AAB46383. (PubMed:1313714)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti6 – 61S → A.1 Publication
    VAR_000604
    Natural varianti177 – 1771G → A.2 Publications
    Corresponds to variant rs3013886 [ dbSNP | Ensembl ].
    VAR_030630

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei138 – 1414DYGS → GQQG in isoform 2. 1 PublicationVSP_056397
    Alternative sequencei142 – 327186Missing in isoform 2. 1 PublicationVSP_056398Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16662 mRNA. Translation: CAA34650.1.
    M81844 mRNA. Translation: AAB46383.1.
    AK300158 mRNA. Translation: BAG61941.1.
    AL591684 Genomic DNA. Translation: CAH72203.1.
    BC004376 mRNA. Translation: AAH04376.1.
    BC073755 mRNA. Translation: AAH73755.1.
    CCDSiCCDS31194.1.
    PIRiS06476. LUHU8.
    RefSeqiNP_001035173.1. NM_001040084.2.
    NP_001092315.2. NM_001098845.2.
    NP_001258631.1. NM_001271702.1.
    NP_001258632.1. NM_001271703.1.
    UniGeneiHs.535306.
    Hs.705389.
    Hs.744068.

    Genome annotation databases

    EnsembliENST00000535874; ENSP00000445192; ENSG00000165390.
    ENST00000585281; ENSP00000462880; ENSG00000265190.
    GeneIDi653145.
    728113.
    KEGGihsa:244.
    hsa:653145.
    hsa:728113.

    Polymorphism databases

    DMDMi215274181.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16662 mRNA. Translation: CAA34650.1 .
    M81844 mRNA. Translation: AAB46383.1 .
    AK300158 mRNA. Translation: BAG61941.1 .
    AL591684 Genomic DNA. Translation: CAH72203.1 .
    BC004376 mRNA. Translation: AAH04376.1 .
    BC073755 mRNA. Translation: AAH73755.1 .
    CCDSi CCDS31194.1.
    PIRi S06476. LUHU8.
    RefSeqi NP_001035173.1. NM_001040084.2.
    NP_001092315.2. NM_001098845.2.
    NP_001258631.1. NM_001271702.1.
    NP_001258632.1. NM_001271703.1.
    UniGenei Hs.535306.
    Hs.705389.
    Hs.744068.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W3W X-ray 1.99 A 1-327 [» ]
    1W45 X-ray 2.51 A/B 1-327 [» ]
    ProteinModelPortali P13928.
    SMRi P13928. Positions 8-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106745. 7 interactions.
    575558. 3 interactions.
    IntActi P13928. 3 interactions.
    MINTi MINT-1892975.
    STRINGi 9606.ENSP00000341674.

    PTM databases

    PhosphoSitei P13928.

    Polymorphism databases

    DMDMi 215274181.

    Proteomic databases

    MaxQBi P13928.
    PaxDbi P13928.
    PRIDEi P13928.

    Protocols and materials databases

    DNASUi 244.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000535874 ; ENSP00000445192 ; ENSG00000165390 .
    ENST00000585281 ; ENSP00000462880 ; ENSG00000265190 .
    GeneIDi 653145.
    728113.
    KEGGi hsa:244.
    hsa:653145.
    hsa:728113.

    Organism-specific databases

    CTDi 653145.
    728113.
    GeneCardsi GC10P048255.
    HGNCi HGNC:546. ANXA8.
    MIMi 602396. gene.
    neXtProti NX_P13928.
    PharmGKBi PA24836.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG267770.
    HOGENOMi HOG000158803.
    HOVERGENi HBG061815.
    InParanoidi P13928.
    KOi K17096.
    OMAi MAPHYFA.
    PhylomeDBi P13928.
    TreeFami TF105452.

    Miscellaneous databases

    EvolutionaryTracei P13928.
    NextBioi 972.
    PROi P13928.
    SOURCEi Search...

    Gene expression databases

    Bgeei P13928.
    CleanExi HS_ANXA8.
    Genevestigatori P13928.

    Family and domain databases

    Gene3Di 1.10.220.10. 4 hits.
    InterProi IPR001464. Annexin.
    IPR018502. Annexin_repeat.
    IPR018252. Annexin_repeat_CS.
    IPR009115. AnnexinVIII.
    [Graphical view ]
    Pfami PF00191. Annexin. 4 hits.
    [Graphical view ]
    PRINTSi PR00196. ANNEXIN.
    PR01808. ANNEXINVIII.
    SMARTi SM00335. ANX. 4 hits.
    [Graphical view ]
    PROSITEi PS00223. ANNEXIN. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vascular anticoagulant beta: a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning, expression and comparison with VAC-alpha."
      Hauptmann R., Maurer-Fogy I., Krystek E., Bodo G., Andree H., Reutelingsperger C.P.M.
      Eur. J. Biochem. 185:63-71(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ALA-6 AND ALA-177.
      Tissue: Placenta.
    2. "Specific expression of the annexin VIII gene in acute promyelocytic leukemia."
      Chang K.S., Wang G., Freireich E.J., Daly M., Naylor S.L., Trujillo J.M., Stass S.A.
      Blood 79:1802-1810(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Placenta.
    4. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-177.
      Tissue: Lung and Pancreas.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS), CALCIUM-BINDING SITES.

    Entry informationi

    Entry nameiANXA8_HUMAN
    AccessioniPrimary (citable) accession number: P13928
    Secondary accession number(s): A6NLM1
    , B4DTC9, Q5VTM4, Q6GMY3, Q9BT34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 150 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3