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P13927 (EFTU_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor Tu

Short name=EF-Tu
Gene names
Name:tuf
Ordered Locus Names:MG451
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00118

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00118.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionElongation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: InterPro

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Elongation factor Tu HAMAP-Rule MF_00118
PRO_0000091346

Regions

Nucleotide binding19 – 268GTP By similarity
Nucleotide binding81 – 855GTP By similarity
Nucleotide binding136 – 1394GTP By similarity

Experimental info

Sequence conflict2201T → N in AAD12520. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P13927 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 3F62C644A40E49DA

FASTA39442,990
        10         20         30         40         50         60 
MAREKFDRSK PHVNVGTIGH IDHGKTTLTA AICTVLAKEG KSAATRYDEI DKAPEEKARG 

        70         80         90        100        110        120 
ITINSAHVEY SSDKRHYAHV DCPGHADYIK NMITGAAQMD GAILVVSATD SVMPQTREHI 

       130        140        150        160        170        180 
LLARQVGVPK MVVFLNKCDI ASDEEVQELV AEEVRDLLTS YGFDGKNTPI IYGSALKALE 

       190        200        210        220        230        240 
GDPKWEAKIH DLIKAVDEWI PTPTREVDKP FLLAIEDTMT ITGRGTVVTG RVERGELKVG 

       250        260        270        280        290        300 
QEVEIVGLKP IRKAVVTGIE MFKKELDSAM AGDNAGVLLR GVERKEVERG QVLAKPGSIK 

       310        320        330        340        350        360 
PHKKFKAEIY ALKKEEGGRH TGFLNGYRPQ FYFRTTDVTG SIALAENTEM VLPGDNASIT 

       370        380        390 
VELIAPIACE KGSKFSIREG GRTVGAGTVT EVLE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the tuf gene from Mycoplasma genitalium."
Inamine J.M., Loechel S., Hu P.-C.
Nucleic Acids Res. 17:10127-10127(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]"The minimal gene complement of Mycoplasma genitalium."
Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. expand/collapse author list , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.
Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]"A survey of the Mycoplasma genitalium genome by using random sequencing."
Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 196-309.
Strain: ATCC 33530 / G-37 / NCTC 10195.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16463 Genomic DNA. Translation: CAA34483.1.
L43967 Genomic DNA. Translation: AAC72471.1.
U02255 Genomic DNA. Translation: AAD12520.1.
PIREFYMTG. S14909.
RefSeqNP_073121.1. NC_000908.2.

3D structure databases

ProteinModelPortalP13927.
SMRP13927. Positions 2-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_451.

Proteomic databases

PRIDEP13927.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC72471; AAC72471; MG_451.
GeneID875605.
KEGGmge:MG_451.
PATRIC20010492. VBIMycGen98045_0519.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0050.
KOK02358.
OMAGTEMCMP.
OrthoDBEOG6R5C6X.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-504-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00118_B. EF_Tu_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEFTU_MYCGE
AccessionPrimary (citable) accession number: P13927
Secondary accession number(s): Q49360
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names