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Protein

Plasminogen activator inhibitor 1

Gene

SERPINE1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei369 – 3702Reactive bond

GO - Molecular functioni

  • enzyme inhibitor activity Source: AgBase
  • serine-type endopeptidase inhibitor activity Source: GO_Central
  • zymogen binding Source: AgBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-BTA-114608. Platelet degranulation.
R-BTA-3000178. ECM proteoglycans.
R-BTA-75205. Dissolution of Fibrin Clot.

Protein family/group databases

MEROPSiI04.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:SERPINE1
Synonyms:PAI1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 402379Plasminogen activator inhibitor 1PRO_0000032498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP13909.
PRIDEiP13909.

Expressioni

Tissue specificityi

Vascular endothelial cells may be the primary site of synthesis of plasma PAI1.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation (By similarity).By similarity

GO - Molecular functioni

  • zymogen binding Source: AgBase

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019232.

Structurei

3D structure databases

ProteinModelPortaliP13909.
SMRiP13909. Positions 32-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00840000129734.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP13909.
KOiK03982.
OMAiIVNDWVK.
OrthoDBiEOG7327PB.
TreeFamiTF352620.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMSPVFACL ALGLALIFGE GSASYQPQSA AASLATDFGV KVFQQVVRAS
60 70 80 90 100
KDRNVVFSPY GVASVLAMLQ LTTGGETRQQ IQEAMQFKIE EKGMAPAFHR
110 120 130 140 150
LYKELMGPWN KDEISTADAI FVQRDLELVH GFMPNFFRLF RTTVKQVDFS
160 170 180 190 200
EVERARFIVN DWVKRHTKGM ISDLLGEGAV DQLTRLVLVN ALYFNGQWKM
210 220 230 240 250
PFPESNTHHR LFHKSDGSTI SVPMMAQTNK FNYTEFTTPD GRYYDILELP
260 270 280 290 300
YHGNTLSMLI AAPYEKEVPL SALTSILDAE LISQWKGNMT RLTRLLVLPK
310 320 330 340 350
FSLETEIDLR RPLENLGMTD MFRPSQADFS SFSDQEFLYV SQALQKVKIE
360 370 380 390 400
VNESGTLASS STALVVSARM APEEIIMDRP FLFVVRHNPT GTVLFMGQVM

EP
Length:402
Mass (Da):45,371
Last modified:January 1, 1990 - v1
Checksum:i9053617333C7D130
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501S → L (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16383 mRNA. Translation: CAA34419.1.
BT025406 mRNA. Translation: ABF57362.1.
BC103451 mRNA. Translation: AAI03452.1.
X52906 mRNA. Translation: CAA37094.1.
PIRiS06745.
RefSeqiNP_776562.1. NM_174137.2.
XP_010817781.1. XM_010819479.2.
UniGeneiBt.5229.

Genome annotation databases

EnsembliENSBTAT00000019232; ENSBTAP00000019232; ENSBTAG00000014465.
GeneIDi281375.
KEGGibta:281375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16383 mRNA. Translation: CAA34419.1.
BT025406 mRNA. Translation: ABF57362.1.
BC103451 mRNA. Translation: AAI03452.1.
X52906 mRNA. Translation: CAA37094.1.
PIRiS06745.
RefSeqiNP_776562.1. NM_174137.2.
XP_010817781.1. XM_010819479.2.
UniGeneiBt.5229.

3D structure databases

ProteinModelPortaliP13909.
SMRiP13909. Positions 32-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019232.

Protein family/group databases

MEROPSiI04.020.

Proteomic databases

PaxDbiP13909.
PRIDEiP13909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000019232; ENSBTAP00000019232; ENSBTAG00000014465.
GeneIDi281375.
KEGGibta:281375.

Organism-specific databases

CTDi5054.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00840000129734.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP13909.
KOiK03982.
OMAiIVNDWVK.
OrthoDBiEOG7327PB.
TreeFamiTF352620.

Enzyme and pathway databases

ReactomeiR-BTA-114608. Platelet degranulation.
R-BTA-3000178. ECM proteoglycans.
R-BTA-75205. Dissolution of Fibrin Clot.

Miscellaneous databases

NextBioi20805379.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA for bovine type 1 plasminogen activator inhibitor (PAI-1)."
    Mimuro J., Sawdey M., Hatiori M., Loskutoff D.J.
    Nucleic Acids Res. 17:8872-8872(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.
  4. "Bovine endothelial cell plasminogen activator inhibitor. Purification and heat activation."
    Katagiri K., Okada K., Hattori H., Yano M.
    Eur. J. Biochem. 176:81-87(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-63.
  5. "Transforming growth factor-beta 1 modulates basic fibroblast growth factor-induced proteolytic and angiogenic properties of endothelial cells in vitro."
    Pepper M.S., Belin D., Montesano R., Orci L., Vassalli J.-D.
    J. Cell Biol. 111:743-755(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 153-235.
    Tissue: Adrenal cortex.

Entry informationi

Entry nameiPAI1_BOVIN
AccessioniPrimary (citable) accession number: P13909
Secondary accession number(s): Q3ZBB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 11, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.