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P13906

- MTB1_LYSSH

UniProt

P13906 - MTB1_LYSSH

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Protein
Modification methylase BspRI
Gene
bspRIM
Organism
Lysinibacillus sphaericus (Bacillus sphaericus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BspRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei156 – 1561S-methylcysteine intermediate

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3322. M.BspRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BspRI (EC:2.1.1.37)
Short name:
M.BspRI
Alternative name(s):
Cytosine-specific methyltransferase BspRI
Gene namesi
Name:bspRIM
OrganismiLysinibacillus sphaericus (Bacillus sphaericus)
Taxonomic identifieri1421 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Modification methylase BspRI
PRO_0000087862Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811S-methylcysteine; by autocatalysis1 Publication

Post-translational modificationi

In the absence of DNA, can self-methylate two cysteine residues.

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP13906.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 408351SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 2 hits.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13906-1 [UniParc]FASTAAdd to Basket

« Hide

MAIKINEKGR GKFKPAPTYE KEEVRQLLME KINEEMEAVA TATSDISNDE    50
IQYKSDKFNV LSLFCGAGGL DLGFELAGLE QSLGTDKALE AFKDRDVYNA 100
IRHESVFHTV YANDIFSEAL QTYEKNMPNH VFIHEKDIRK IKEFPSANLV 150
IGGFPCPGFS EAGPRLVDDE RNFLYIHFIR CLMQVQPEIF VAENVKGMMT 200
LGGGEVFRQI VEDFGAAGYR VEARLLNARD YGVPQIRERV IIVGVRNDID 250
FNYEYPEITH GNEEGLKPYV TLEEAIGDLS LDPGPYFTGS YSTIFMSRNR 300
KKKWTDQSFT IQASGRQAPI HPGGLPMEKV DKNKWIFPDG EENHRRLSVK 350
EIKRIQTFPD WYEFSDGGNM KVSVNNRLDK QYKQIGNAVP VFLARAVAKS 400
IAQFAADYLK DNHPHEAPQM KLFI 424
Length:424
Mass (Da):48,212
Last modified:June 15, 2010 - v3
Checksum:i1DED6A45DB0A4FCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15758 Genomic DNA. Translation: CAA33764.2.
PIRiS07792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15758 Genomic DNA. Translation: CAA33764.2 .
PIRi S07792.

3D structure databases

ProteinModelPortali P13906.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3322. M.BspRI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 2 hits.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of the Bacillus sphaericus R modification methylase gene."
    Posfai G., Kiss A., Erdei S., Posfai J., Venetianer P.
    J. Mol. Biol. 170:597-610(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: SEQUENCE REVISION TO 322, PARTIAL PROTEIN SEQUENCE, AUTOMETHYLATION AT CYS-181.
  3. Rasko T., Der A., Klement E., Posfai E., Medzihradszky K.F., Marshak D.R., Roberts R.J., Kiss A.
    Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 394.

Entry informationi

Entry nameiMTB1_LYSSH
AccessioniPrimary (citable) accession number: P13906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 15, 2010
Last modified: June 11, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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