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Protein

Modification methylase BspRI

Gene

bspRIM

Organism
Lysinibacillus sphaericus (Bacillus sphaericus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BspRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156S-methylcysteine intermediate1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3322 M.BspRI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BspRI (EC:2.1.1.37)
Short name:
M.BspRI
Alternative name(s):
Cytosine-specific methyltransferase BspRI
Gene namesi
Name:bspRIM
OrganismiLysinibacillus sphaericus (Bacillus sphaericus)
Taxonomic identifieri1421 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878621 – 424Modification methylase BspRIAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181S-methylcysteine; by autocatalysis1 Publication1

Post-translational modificationi

In the absence of DNA, can self-methylate two cysteine residues.

Keywords - PTMi

Methylation

PTM databases

iPTMnetiP13906

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP13906
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 408SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST351

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 2 hits
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P13906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKINEKGR GKFKPAPTYE KEEVRQLLME KINEEMEAVA TATSDISNDE
60 70 80 90 100
IQYKSDKFNV LSLFCGAGGL DLGFELAGLE QSLGTDKALE AFKDRDVYNA
110 120 130 140 150
IRHESVFHTV YANDIFSEAL QTYEKNMPNH VFIHEKDIRK IKEFPSANLV
160 170 180 190 200
IGGFPCPGFS EAGPRLVDDE RNFLYIHFIR CLMQVQPEIF VAENVKGMMT
210 220 230 240 250
LGGGEVFRQI VEDFGAAGYR VEARLLNARD YGVPQIRERV IIVGVRNDID
260 270 280 290 300
FNYEYPEITH GNEEGLKPYV TLEEAIGDLS LDPGPYFTGS YSTIFMSRNR
310 320 330 340 350
KKKWTDQSFT IQASGRQAPI HPGGLPMEKV DKNKWIFPDG EENHRRLSVK
360 370 380 390 400
EIKRIQTFPD WYEFSDGGNM KVSVNNRLDK QYKQIGNAVP VFLARAVAKS
410 420
IAQFAADYLK DNHPHEAPQM KLFI
Length:424
Mass (Da):48,212
Last modified:June 15, 2010 - v3
Checksum:i1DED6A45DB0A4FCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15758 Genomic DNA Translation: CAA33764.2
PIRiS07792

Similar proteinsi

Entry informationi

Entry nameiMTB1_LYSSH
AccessioniPrimary (citable) accession number: P13906
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 15, 2010
Last modified: March 28, 2018
This is version 97 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
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Main funding by: National Institutes of Health