P13906 (MTB1_LYSSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BspRI
Short name=M.BspRI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase BspRI

Gene names

Name:

bspRIM

Organism

Lysinibacillus sphaericus (Bacillus sphaericus)

Taxonomic identifier

1421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Lysinibacillus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BspRI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Monomer.
Post-translational modification	In the absence of DNA, can self-methylate two cysteine residues.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Methylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Modification methylase BspRI		PRO_0000087862
Sites
	Active site	156	1	S-methylcysteine intermediate
Amino acid modifications
	Modified residue	181	1	S-methylcysteine; by autocatalysis

Sequences

	Sequence		Length	Mass (Da)
	P13906 [UniParc]. Last modified June 15, 2010. Version 3. Checksum: 1DED6A45DB0A4FCF	FASTA	424	48,212
	10 20 30 40 50 60 MAIKINEKGR GKFKPAPTYE KEEVRQLLME KINEEMEAVA TATSDISNDE IQYKSDKFNV 70 80 90 100 110 120 LSLFCGAGGL DLGFELAGLE QSLGTDKALE AFKDRDVYNA IRHESVFHTV YANDIFSEAL 130 140 150 160 170 180 QTYEKNMPNH VFIHEKDIRK IKEFPSANLV IGGFPCPGFS EAGPRLVDDE RNFLYIHFIR 190 200 210 220 230 240 CLMQVQPEIF VAENVKGMMT LGGGEVFRQI VEDFGAAGYR VEARLLNARD YGVPQIRERV 250 260 270 280 290 300 IIVGVRNDID FNYEYPEITH GNEEGLKPYV TLEEAIGDLS LDPGPYFTGS YSTIFMSRNR 310 320 330 340 350 360 KKKWTDQSFT IQASGRQAPI HPGGLPMEKV DKNKWIFPDG EENHRRLSVK EIKRIQTFPD 370 380 390 400 410 420 WYEFSDGGNM KVSVNNRLDK QYKQIGNAVP VFLARAVAKS IAQFAADYLK DNHPHEAPQM KLFI

References

[1]	"Structure of the Bacillus sphaericus R modification methylase gene." Posfai G., Kiss A., Erdei S., Posfai J., Venetianer P. J. Mol. Biol. 170:597-610(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Self-methylation of BspRI DNA-methyltransferase." Szilak L., Finta C., Patthy A., Venetianer P., Kiss A. Nucleic Acids Res. 22:2876-2881(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 322, PARTIAL PROTEIN SEQUENCE, AUTOMETHYLATION.
[3]	Rasko T., Der A., Klement E., Posfai E., Medzihradszky K.F., Marshak D.R., Roberts R.J., Kiss A. Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 394.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15758 Genomic DNA. Translation: CAA33764.2.
PIR	S07792.
3D structure databases
ProteinModelPortal	P13906.
ModBase	Search...
Protein family/group databases
REBASE	3322. M.BspRI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_LYSSH

Accession

Primary (citable) accession number: P13906

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 15, 2010
Last modified:	April 3, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program