P13876 (PB2_INBAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polymerase basic protein 2

Alternative name(s):
RNA-directed RNA polymerase subunit P3

Gene names

Name:

PB2

Organism

Influenza B virus (strain B/Ann Arbor/1/1966 [wild-type])

Taxonomic identifier

11523 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus B › Influenza B virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	770 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication By similarity.
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Subcellular location	Virion. Host nucleus. Host mitochondrion By similarity.
Sequence similarities	Belongs to the influenza viruses PB2 family.

Ontologies

Keywords
Biological process	Cap snatching mRNA capping mRNA processing
Cellular component	Host mitochondrion Host nucleus Virion
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 770	770	Polymerase basic protein 2		PRO_0000078845
Regions
	Motif	740 – 743	4	Nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13876 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: E07E91EC92744EA5	FASTA	770	88,025
	10 20 30 40 50 60 MTLAKIELLK QLLRDNEAKT VLKQTTVDQY NIIRKFNTSR IERNPSLRMK WAMCSNFPLA 70 80 90 100 110 120 LTKGDMANRI PLEYKGIRLK TNAEDIGTKG QMCSIAAVTW WNTYGPIGDT EGFEKVYESF 130 140 150 160 170 180 FLRKMRLDNA TWGRITFGPV ERVRKRVLLN PLTKEMPPDE ASNVIMEILF PKEAGIPRES 190 200 210 220 230 240 TWMHRELIKE KREKLKGTMI TPIVLAYMLE RELVARRRFL PVAGATSAEF IEMLHCLQGE 250 260 270 280 290 300 NWRQIYHPGG NKLTESRSQS MIVACRKIVR RSIVASNPLE LAVEIANKTV IDTEPLKSCL 310 320 330 340 350 360 AAIDGGDVAC DIIRAALGLK IRQRQRFGRL ELKRISGRGF KNDEEILIGN GTIQKIGIWD 370 380 390 400 410 420 GEEEFHVRCG ECRGILKKSK MRMEKLLINS AKKEDMKDLI ILCMVFSQDT RMFQGVRGEI 430 440 450 460 470 480 NFLNRAGQLL SPMYQLQRYF LNRSNDLFDQ WGYEEPPKAS ELHGINELMN ASDYTLKGVV 490 500 510 520 530 540 VTKNVIDDFS STETEKVSIT KNLSLIKRTG EVIMGANDVS ELESQAQLMI TYDTPKMWEM 550 560 570 580 590 600 GTTKELVQNT YQWVLKNLVT LKAQFLLGKE DMFQWDAFEA FESIIPQKMA GQYSGFARAV 610 620 630 640 650 660 LKQMRDQEVM KTDQFIKLLP FCFSPPKLRS NGEPYQFLRL MLKGGGENFI EVRKGSPLFS 670 680 690 700 710 720 YNPQTEVLTI CGRMMSLKGK IEDEERNRSM GNAVLAGFLV SGKYDPDLGD FKTIEELEKL 730 740 750 760 770 KPGEKANILL YQGKPVKVVK RKRYSALSND ISQGIKRQRM TVESMGWALS

References

[1]	"Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes." Deborde D.C., Donabedian A.M., Herlocher M.L., Naeve C.W., Maassab H.F. Virology 163:429-443(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20168 Genomic RNA. Translation: AAA43771.1.
PIR	P3IVBW. B28604.
3D structure databases
ProteinModelPortal	P13876.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001591. RNA_pol_PB2_orthomyxovir. [Graphical view]
Pfam	PF00604. Flu_PB2. 1 hit. [Graphical view]
ProDom	PD001667. RNA_pol_PB2_orthomyxovir. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PB2_INBAD

Accession

Primary (citable) accession number: P13876

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program