P13872 (RDRP_INBAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase catalytic subunit
EC=2.7.7.48

Alternative name(s):
Polymerase basic protein 1
Short name=PB1
RNA-directed RNA polymerase subunit P1

Gene names

Name:

PB1

Organism

Influenza B virus (strain B/Ann Arbor/1/1966 [wild-type])

Taxonomic identifier

11523 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus B › Influenza B virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Sequence similarities	Belongs to the influenza viruses polymerase PB1 family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 752	752	RNA-directed RNA polymerase catalytic subunit		PRO_0000078773
Regions
	Domain	286 – 482	197	RdRp catalytic
Experimental info
	Sequence conflict	239	1	R → E in AAA43768. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P13872 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 18240F96457A7C00	FASTA	752	84,322
	10 20 30 40 50 60 MNINPYFLFI DVPIQAAIST TFPYTGVPPY SHGTGTGYTI DTVIRTHEYS NKGKQYISDV 70 80 90 100 110 120 TGCAMVDPTN GPLPEDNEPS AYAQLDCVLE ALDRMDEEHP GLFQAASQNA MEALMVTTVD 130 140 150 160 170 180 KLTQGRQTFD WTVCRNQPAA TALNTTITSF RLNDLNGADK GGLVPFCQDI IDSLDKPEMT 190 200 210 220 230 240 FFSVKNIKKK LPAKNRKGFL IKRIPMKVKD RITRVEYIKR ALSLNTMTKD AERGKLKRRA 250 260 270 280 290 300 IATAGIQIRG FVLVVENLAK NICENLEQSG LPVGGNEKKA KLSNAVAKML SNCPPGGISM 310 320 330 340 350 360 TVTGDNTKWN ECLNPRIFLA MTERITRDSP IWFRDFCSIA PVLFSNKIAR LGKGFMITSK 370 380 390 400 410 420 TKRLKAQIPC PDLFNIPLER YNEETRAKLK KLKPFFNEEG TASLSPGMMM GMFNMLSTVL 430 440 450 460 470 480 GVAALGIKNI GNREYLWDGL QSSDDFALFV NAKDEETCME GINDFYRTCK LLGINMSKKK 490 500 510 520 530 540 SYCNETGMFE FTSMFYRDGF VSNFAMELPS FGVAGVNESA DMAIGMTIIK NNMINNGMGP 550 560 570 580 590 600 ATAQTAIQLF IADYRYTYKC HRGDSKVEGK RMKIIKELWE NTKGRDGLLV ADGGPNIYNL 610 620 630 640 650 660 RNLHIPEIVL KYNLMDPEYK GRLLHPQNPF VGHLSIEGIK EADITPAHGP IKKMDYDAVS 670 680 690 700 710 720 GTHSWRTKRN RSILNTDQRN MILEEQCYAK CCNLFEACFN SASYRKPVGQ HSMLEAMAHR 730 740 750 LRMDARLDYE SGRMSKDDFE KAMAHLGEIG HI

References

[1]	"Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes." Deborde D.C., Donabedian A.M., Herlocher M.L., Naeve C.W., Maassab H.F. Virology 163:429-443(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Nucleotide sequences of the PA and PB1 genes of B/Ann Arbor/1/66 virus: comparison with genes of B/Lee/40 and type A influenza viruses." Deborde D.C., Herlocher M.L., Maassab H.F. Virus Res. 8:33-41(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20170 Genomic RNA. Translation: AAA43770.1. M20479 Genomic RNA. Translation: AAA43768.1.
PIR	P1IVBW. D28604.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007099. RNA-dir_pol_NSvirus. IPR001407. RNA_pol_PB1_influenza. [Graphical view]
Pfam	PF00602. Flu_PB1. 1 hit. [Graphical view]
PIRSF	PIRSF000827. RdRPol_OMV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_INBAD

Accession

Primary (citable) accession number: P13872
Secondary accession number(s): Q84079

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program