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Protein

Cyclin-dependent kinase 1

Gene

CDK1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Thr-14 and Tyr-15 are phosphorylated maximally during G2 phase, but dephosphorylated abruptly at the G2/M transition. Phosphorylation at Thr-14 and Tyr-15 inactivates the enzyme. During M phase it is also phosphorylated on Thr-161. Finally during G1 phase it is phosphorylated on Ser-277.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to heat Source: AgBase
  • double-strand break repair via homologous recombination Source: Reactome
  • G2/M transition of mitotic cell cycle Source: AgBase
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1306.
ReactomeiR-GGA-265976. Homologous DNA pairing and strand exchange.
R-GGA-351451. Homologous recombination repair of replication-dependent double-strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:CDK1
Synonyms:CDC2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857281 – 303Cyclin-dependent kinase 1Add BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14Phosphothreonine1 Publication1
Modified residuei15Phosphotyrosine; by WEE1 and WEE21 Publication1
Modified residuei161Phosphothreonine; by CAKBy similarity1
Modified residuei277Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13863.
PRIDEiP13863.

PTM databases

iPTMnetiP13863.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.

Protein-protein interaction databases

BioGridi676512. 1 interactor.
STRINGi9031.ENSGALP00000004867.

Structurei

3D structure databases

ProteinModelPortaliP13863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 287Protein kinasePROSITE-ProRule annotationAdd BLAST284

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOVERGENiHBG014652.
InParanoidiP13863.
KOiK02087.
PhylomeDBiP13863.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR
60 70 80 90 100
EISLLKELHH PNIVCLQDVL MQDARLYLIF EFLSMDLKKY LDTIPSGQYL
110 120 130 140 150
DRSRVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG VIKLADFGLA
160 170 180 190 200
RAFGIPVRVY THEVVTLWYR SPEVLLGSAL YSTPVDIWSI GTIFAELATK
210 220 230 240 250
KPLFHGDSEI DQLFRIFRAL GTPNNDVWPD VESLQDYKNT FPKWKPGSLG
260 270 280 290 300
THVQNLDEDG LDLLSKMLIY DPAKRISGKM ALNHPYFDDL DKSTLPANLI

KKF
Length:303
Mass (Da):34,688
Last modified:January 1, 1990 - v1
Checksum:i976740ECC4741D69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16881 mRNA. Translation: CAA34764.1.
PIRiS06011.
RefSeqiNP_990645.1. NM_205314.1.
UniGeneiGga.726.

Genome annotation databases

GeneIDi396252.
KEGGigga:396252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16881 mRNA. Translation: CAA34764.1.
PIRiS06011.
RefSeqiNP_990645.1. NM_205314.1.
UniGeneiGga.726.

3D structure databases

ProteinModelPortaliP13863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676512. 1 interactor.
STRINGi9031.ENSGALP00000004867.

PTM databases

iPTMnetiP13863.

Proteomic databases

PaxDbiP13863.
PRIDEiP13863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396252.
KEGGigga:396252.

Organism-specific databases

CTDi983.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOVERGENiHBG014652.
InParanoidiP13863.
KOiK02087.
PhylomeDBiP13863.

Enzyme and pathway databases

BRENDAi2.7.11.22. 1306.
ReactomeiR-GGA-265976. Homologous DNA pairing and strand exchange.
R-GGA-351451. Homologous recombination repair of replication-dependent double-strand breaks.

Miscellaneous databases

PROiP13863.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK1_CHICK
AccessioniPrimary (citable) accession number: P13863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 5, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.