Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P13863

- CDK1_CHICK

UniProt

P13863 - CDK1_CHICK

Protein

Cyclin-dependent kinase 1

Gene

CDK1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Thr-14 and Tyr-15 are phosphorylated maximally during G2 phase, but dephosphorylated abruptly at the G2/M transition. Phosphorylation at Thr-14 and Tyr-15 inactivates the enzyme. During M phase it is also phosphorylated on Thr-161. Finally during G1 phase it is phosphorylated on Ser-277.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA repair Source: Reactome
    2. double-strand break repair Source: Reactome
    3. double-strand break repair via homologous recombination Source: Reactome
    4. mitotic nuclear division Source: UniProtKB-KW
    5. regulation of cell cycle Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 1306.
    ReactomeiREACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
    REACT_115626. Homologous DNA pairing and strand exchange.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2 homolog
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:CDK1
    Synonyms:CDC2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Cyclin-dependent kinase 1PRO_0000085728Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141Phosphothreonine1 Publication
    Modified residuei15 – 151Phosphotyrosine; by WEE1 and WEE21 Publication
    Modified residuei161 – 1611Phosphothreonine; by CAKBy similarity
    Modified residuei277 – 2771Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP13863.
    PRIDEiP13863.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.

    Protein-protein interaction databases

    BioGridi676512. 1 interaction.
    STRINGi9031.ENSGALP00000004867.

    Structurei

    3D structure databases

    ProteinModelPortaliP13863.
    SMRiP13863. Positions 1-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG014652.
    InParanoidiP13863.
    KOiK02087.
    PhylomeDBiP13863.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13863-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR    50
    EISLLKELHH PNIVCLQDVL MQDARLYLIF EFLSMDLKKY LDTIPSGQYL 100
    DRSRVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG VIKLADFGLA 150
    RAFGIPVRVY THEVVTLWYR SPEVLLGSAL YSTPVDIWSI GTIFAELATK 200
    KPLFHGDSEI DQLFRIFRAL GTPNNDVWPD VESLQDYKNT FPKWKPGSLG 250
    THVQNLDEDG LDLLSKMLIY DPAKRISGKM ALNHPYFDDL DKSTLPANLI 300
    KKF 303
    Length:303
    Mass (Da):34,688
    Last modified:January 1, 1990 - v1
    Checksum:i976740ECC4741D69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16881 mRNA. Translation: CAA34764.1.
    PIRiS06011.
    RefSeqiNP_990645.1. NM_205314.1.
    UniGeneiGga.726.

    Genome annotation databases

    GeneIDi396252.
    KEGGigga:396252.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16881 mRNA. Translation: CAA34764.1 .
    PIRi S06011.
    RefSeqi NP_990645.1. NM_205314.1.
    UniGenei Gga.726.

    3D structure databases

    ProteinModelPortali P13863.
    SMRi P13863. Positions 1-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 676512. 1 interaction.
    STRINGi 9031.ENSGALP00000004867.

    Proteomic databases

    PaxDbi P13863.
    PRIDEi P13863.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396252.
    KEGGi gga:396252.

    Organism-specific databases

    CTDi 983.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG014652.
    InParanoidi P13863.
    KOi K02087.
    PhylomeDBi P13863.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 1306.
    Reactomei REACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
    REACT_115626. Homologous DNA pairing and strand exchange.

    Miscellaneous databases

    NextBioi 20816304.
    PROi P13863.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and developmental expression of the chicken CDC2 kinase."
      Krek W., Nigg E.A.
      EMBO J. 8:3071-3078(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites."
      Krek W., Nigg E.A.
      EMBO J. 10:305-316(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.

    Entry informationi

    Entry nameiCDK1_CHICK
    AccessioniPrimary (citable) accession number: P13863
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3