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P13863

- CDK1_CHICK

UniProt

P13863 - CDK1_CHICK

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Protein

Cyclin-dependent kinase 1

Gene

CDK1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Thr-14 and Tyr-15 are phosphorylated maximally during G2 phase, but dephosphorylated abruptly at the G2/M transition. Phosphorylation at Thr-14 and Tyr-15 inactivates the enzyme. During M phase it is also phosphorylated on Thr-161. Finally during G1 phase it is phosphorylated on Ser-277.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA repair Source: Reactome
  2. double-strand break repair Source: Reactome
  3. double-strand break repair via homologous recombination Source: Reactome
  4. mitotic nuclear division Source: UniProtKB-KW
  5. regulation of cell cycle Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1306.
ReactomeiREACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
REACT_115626. Homologous DNA pairing and strand exchange.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:CDK1
Synonyms:CDC2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
  3. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Cyclin-dependent kinase 1PRO_0000085728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141Phosphothreonine1 Publication
Modified residuei15 – 151Phosphotyrosine; by WEE1 and WEE21 Publication
Modified residuei161 – 1611Phosphothreonine; by CAKBy similarity
Modified residuei277 – 2771Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13863.
PRIDEiP13863.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.

Protein-protein interaction databases

BioGridi676512. 1 interaction.
STRINGi9031.ENSGALP00000004867.

Structurei

3D structure databases

ProteinModelPortaliP13863.
SMRiP13863. Positions 1-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG014652.
InParanoidiP13863.
KOiK02087.
PhylomeDBiP13863.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13863-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR
60 70 80 90 100
EISLLKELHH PNIVCLQDVL MQDARLYLIF EFLSMDLKKY LDTIPSGQYL
110 120 130 140 150
DRSRVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG VIKLADFGLA
160 170 180 190 200
RAFGIPVRVY THEVVTLWYR SPEVLLGSAL YSTPVDIWSI GTIFAELATK
210 220 230 240 250
KPLFHGDSEI DQLFRIFRAL GTPNNDVWPD VESLQDYKNT FPKWKPGSLG
260 270 280 290 300
THVQNLDEDG LDLLSKMLIY DPAKRISGKM ALNHPYFDDL DKSTLPANLI

KKF
Length:303
Mass (Da):34,688
Last modified:January 1, 1990 - v1
Checksum:i976740ECC4741D69
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16881 mRNA. Translation: CAA34764.1.
PIRiS06011.
RefSeqiNP_990645.1. NM_205314.1.
UniGeneiGga.726.

Genome annotation databases

GeneIDi396252.
KEGGigga:396252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16881 mRNA. Translation: CAA34764.1 .
PIRi S06011.
RefSeqi NP_990645.1. NM_205314.1.
UniGenei Gga.726.

3D structure databases

ProteinModelPortali P13863.
SMRi P13863. Positions 1-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 676512. 1 interaction.
STRINGi 9031.ENSGALP00000004867.

Proteomic databases

PaxDbi P13863.
PRIDEi P13863.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396252.
KEGGi gga:396252.

Organism-specific databases

CTDi 983.

Phylogenomic databases

eggNOGi COG0515.
HOVERGENi HBG014652.
InParanoidi P13863.
KOi K02087.
PhylomeDBi P13863.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 1306.
Reactomei REACT_115585. Homologous recombination repair of replication-dependent double-strand breaks.
REACT_115626. Homologous DNA pairing and strand exchange.

Miscellaneous databases

NextBioi 20816304.
PROi P13863.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and developmental expression of the chicken CDC2 kinase."
    Krek W., Nigg E.A.
    EMBO J. 8:3071-3078(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites."
    Krek W., Nigg E.A.
    EMBO J. 10:305-316(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiCDK1_CHICK
AccessioniPrimary (citable) accession number: P13863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3