P13858 (THEM_MALSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Thermomycolin EC=3.4.21.65 Alternative name(s): Thermomycolase
Organism	Malbranchea sulfurea
Taxonomic identifier	5041 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Malbranchea

Protein attributes

Sequence length	34 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is an extracellular proteinase with a general specificity for apolar residues.
Catalytic activity	Rather non-specific hydrolysis of proteins. Preferential cleavage: -Ala-|-Xaa-, -Tyr-|-Xaa-, -Phe-|-Xaa- in small molecular substrates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S8 family.
Biophysicochemical properties	Temperature dependence: Thermostable.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›34	›34	Thermomycolin		PRO_0000076420
Sites
	Active site	33	1	Charge relay system Ref.1
Experimental info
	Sequence uncertainty	6	1
	Sequence uncertainty	10	1
	Sequence uncertainty	17	1
	Sequence uncertainty	31	1
	Non-adjacent residues	28 – 29	2
	Non-terminal residue	34	1

Sequences

	Sequence		Length	Mass (Da)
	P13858 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 147F90B412F014E6	FASTA	34	3,708
	10 20 30 ALVTQSNAPS WGLGRISNRQ AGIRDYHYLS GTSM

References

[1]	"Thermomycolin." Gaucher G.M., Stevenson K.J. Methods Enzymol. 45:415-433(1976) [PubMed: 1012007] [Abstract] Cited for: PROTEIN SEQUENCE, ACTIVE SITE.
[2]	"Physiochemical properties of thermomycolase, the thermostable, extracellular, serine protease of the fungus Malbranchea pulchella." Voordouw G., Gaucher G.M., Roche R.S. Can. J. Biochem. 52:981-990(1974) [PubMed: 4429868] [Abstract] Cited for: CHARACTERIZATION.
[3]	"The substrate specificity of thermomycolase, an extracellular serine proteinase from the thermophilic fungus Malbranchea pulchella var. sulfurea." Stevenson K.J., Gaucher G.M. Biochem. J. 151:527-542(1975) [PubMed: 3173] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
PIR	A24174.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S08.057.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00136. SUBTILASE_ASP. Partial match. PS00137. SUBTILASE_HIS. Partial match. PS00138. SUBTILASE_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THEM_MALSU

Accession

Primary (citable) accession number: P13858

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program