Reviewed, UniProtKB/Swiss-Prot P13858 (THEM_MALSU)

Names and origin

Protein names	Recommended name:     Thermomycolin     EC=3.4.21.65 Alternative name(s):     Thermomycolase
Organism	Malbranchea sulfurea
Taxonomic identifier	5041 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Malbranchea

Protein attributes

Sequence length	34 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is an extracellular proteinase with a general specificity for apolar residues.
Catalytic activity	Rather non-specific hydrolysis of proteins. Preferential cleavage: -Ala-|-Xaa-, -Tyr-|-Xaa-, -Phe-|-Xaa- in small molecular substrates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S8 family.
biophysicochemical properties	Temperature dependence: Thermostable.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›34	›34	Thermomycolin		PRO_0000076420
Sites
	Active site	33	1	Charge relay system Ref.1
Experimental info
	Sequence uncertainty	6	1
	Sequence uncertainty	10	1
	Sequence uncertainty	17	1
	Sequence uncertainty	31	1
	Non-adjacent residues	28 – 29	2
	Non-terminal residue	34	1

Sequences

	Sequence		Length	Mass (Da)
	P13858-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 147F90B412F014E6	FASTA	34	3,708
	10 20 30 ALVTQSNAPS WGLGRISNRQ AGIRDYHYLS GTSM

References

[1]	"Thermomycolin." Gaucher G.M., Stevenson K.J. Methods Enzymol. 45:415-433(1976) [PubMed: 1012007] [Abstract] Cited for: PROTEIN SEQUENCE, ACTIVE SITE.
[2]	"Physiochemical properties of thermomycolase, the thermostable, extracellular, serine protease of the fungus Malbranchea pulchella." Voordouw G., Gaucher G.M., Roche R.S. Can. J. Biochem. 52:981-990(1974) [PubMed: 4429868] [Abstract] Cited for: CHARACTERIZATION.
[3]	"The substrate specificity of thermomycolase, an extracellular serine proteinase from the thermophilic fungus Malbranchea pulchella var. sulfurea." Stevenson K.J., Gaucher G.M. Biochem. J. 151:527-542(1975) [PubMed: 3173] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
PIR	A24174.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S08.057.
Enzyme and pathway databases
BRENDA	3.4.21.65. 257729.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. [Graphical view]
PROSITE	PS00136. SUBTILASE_ASP. Partial match. PS00137. SUBTILASE_HIS. Partial match. PS00138. SUBTILASE_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THEM_MALSU

Accession

Primary (citable) accession number: P13858

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)