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Protein

Capsid assembly scaffolding protein

Gene

7

Organism
Bacillus phage phi29 (Bacteriophage phi-29)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Scaffolding protein involved in the icosahedric procapsid assembly. Coassembles with the capsid proteins to form the procapsid. The scaffolding protein is found within the capsid as a serie of concentric shells. During DNA packaging, the scaffolding protein molecules are released from the procapsid.2 Publications2 Publications

GO - Molecular functioni

Keywordsi

Molecular functionDNA-binding
Biological processViral capsid assembly, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid assembly scaffolding protein1 Publication
Alternative name(s):
Gene product 7Curated
Short name:
gp7Curated
Head morphogenesis proteinCurated
Protein p7Curated
Scaffold proteinCurated
Gene namesi
Name:7
OrganismiBacillus phage phi29 (Bacteriophage phi-29)
Taxonomic identifieri10756 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaePicovirinaePhi29virus
Virus hostiBacillus subtilis [TaxID: 1423]
Proteomesi
  • UP000001207 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • viral scaffold Source: UniProtKB

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56E → K: Forms procapsids which have incorporated the connector. Defective in producing infectious virions. 1 Publication1
Mutagenesisi69R → E: Fails to incorporate the connector. Defective in producing infectious virions. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065711 – 98Capsid assembly scaffolding proteinAdd BLAST98

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer. Interacts non-specifically with DNA; probably binds DNA in the early stages of DNA packaging.1 Publication

Structurei

Secondary structure

198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 14Combined sources9
Helixi23 – 46Combined sources24
Helixi51 – 59Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NO4X-ray2.20A/B/C/D2-98[»]
1NOHX-ray2.80A/B/C/D2-98[»]
3MTUX-ray2.10E/F2-46[»]
3OA7X-ray2.30A2-50[»]
4IFFX-ray2.30A/B/C/D2-48[»]
4XA1X-ray3.20A/B/C/D1-49[»]
4XA3X-ray2.55A/B1-49[»]
4XA6X-ray3.42A/B/C/D2-50[»]
5CJ1X-ray2.10A/B/C/D/E/F/G/H2-52[»]
5WJBX-ray2.90A/B/C/D2-48[»]
5WLQX-ray3.10A2-48[»]
5WMEX-ray2.30A/B/C/D2-52[»]
ProteinModelPortaliP13848
SMRiP13848
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13848

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili40 – 62Sequence analysisAdd BLAST23

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

OrthoDBiVOG090001HT

Family and domain databases

InterProiView protein in InterPro
IPR038032 Gp7
IPR024374 Phage_phi-29_Gp7
PfamiView protein in Pfam
PF11418 Scaffolding_pro, 1 hit
SUPFAMiSSF90246 SSF90246, 1 hit

Sequencei

Sequence statusi: Complete.

P13848-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLKPEEHED ILNKLLDPEL AQSERTEALQ QLRVNYGSFV SEYNDLTKSH
60 70 80 90
EKLAAEKDDL IVSNSKLFRQ IGLTDKQEED HKKADISETI TIEDLEAK
Length:98
Mass (Da):11,267
Last modified:January 1, 1990 - v1
Checksum:iFF2E7985D2266E14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14782 Genomic DNA Translation: AAA32279.1
X04386 Genomic DNA Translation: CAA27974.1
EU771092 Genomic DNA Translation: ACE96029.1
AJ606486 Genomic DNA Translation: CAE54613.1
PIRiA28923 WMBPF9
RefSeqiYP_002004535.1, NC_011048.1

Genome annotation databases

GeneIDi6446525
KEGGivg:6446525

Similar proteinsi

Entry informationi

Entry nameiSCAF_BPPH2
AccessioniPrimary (citable) accession number: P13848
Secondary accession number(s): B3VMP2, Q70AA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health