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P13838 (LEUK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Leukosialin
Alternative name(s):
Leukocyte sialoglycoprotein
Sialophorin
W3/13 antigen
CD_antigen=CD43
Gene names
Name:Spn
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length378 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response By similarity.

Subunit structure

Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX By similarity. Ref.2

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Post-translational modification

Has a high content of sialic acid and O-linked carbohydrate structures.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide‹1 – 7›7
Chain8 – 378371Leukosialin
PRO_0000021590

Regions

Topological domain8 – 231224Extracellular Potential
Transmembrane232 – 25423Helical; Potential
Topological domain255 – 378124Cytoplasmic Potential

Amino acid modifications

Modified residue2681Phosphoserine By similarity
Modified residue3261Phosphoserine By similarity
Modified residue3301Phosphoserine By similarity
Glycosylation131O-linked (GalNAc...)
Glycosylation151O-linked (GalNAc...)
Glycosylation201O-linked (GalNAc...)
Glycosylation231O-linked (GalNAc...)
Glycosylation251O-linked (GalNAc...)
Glycosylation271O-linked (GalNAc...)
Glycosylation281O-linked (GalNAc...)
Glycosylation291O-linked (GalNAc...)
Glycosylation331O-linked (GalNAc...)
Glycosylation341O-linked (GalNAc...)
Glycosylation361O-linked (GalNAc...)
Glycosylation371O-linked (GalNAc...)
Glycosylation401O-linked (GalNAc...)
Glycosylation1081O-linked (GalNAc...)
Glycosylation1131O-linked (GalNAc...)
Glycosylation1181O-linked (GalNAc...) Potential
Glycosylation1201O-linked (GalNAc...)
Glycosylation1241O-linked (GalNAc...)
Glycosylation1251O-linked (GalNAc...)
Glycosylation1261O-linked (GalNAc...)
Glycosylation1741O-linked (GalNAc...)
Glycosylation1761O-linked (GalNAc...)
Glycosylation1801O-linked (GalNAc...)
Glycosylation1831O-linked (GalNAc...) Potential
Glycosylation1871O-linked (GalNAc...)
Glycosylation1891O-linked (GalNAc...)

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P13838 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 231CC80E8A8A257C

FASTA37838,426
        10         20         30         40         50         60 
WAQVVSQENL PNTMTMLPFT PNSESPSTSE ALSTYSSIAT VPVTEDPKES ISPWGQTTAP 

        70         80         90        100        110        120 
ASSIPLGTPE LSSFFFTSAG ASGNTPVPEL TTSQEVSTEA SLVLFPKSSG VASDPPVTIT 

       130        140        150        160        170        180 
NPATSSAVAS TSLETFKGTS APPVTVTSST MTSGPFVATT VSSETSGPPV TMATGSLGPS 

       190        200        210        220        230        240 
KETHGLSATI ATSSGESSSV AGGTPVFSTK ISTTSTPNPI TTVPPRPGSS GMLLVSMLIA 

       250        260        270        280        290        300 
LTVVLVLVAL LLLWRQRQKR RTGALTLSRG GKRNGTVDAW AGPARVPDEE ATTASGSGGN 

       310        320        330        340        350        360 
KSSGAPETDG SGQRPTLTTF FSRRKSRQGS VALEELKPGT GPNLKGEEEP LVGSEDEAVE 

       370 
TPTSDGPQAK DGAAPQSL 

« Hide

References

[1]"The sequence of rat leukosialin (W3/13 antigen) reveals a molecule with O-linked glycosylation of one third of its extracellular amino acids."
Killeen N., Barclay A.N., Willis A.C., Williams A.F.
EMBO J. 6:4029-4034(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Thymocyte.
[2]"CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor sialoadhesin (Siglec-1)."
van den Berg T.K., Nath D., Ziltener H.J., Vestweber D., Fukuda M., van Die I., Crocker P.R.
J. Immunol. 166:3637-3640(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00090 mRNA. Translation: CAA68281.1.
PIRS00842.
UniGeneRn.11144.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000051894.

Proteomic databases

PaxDbP13838.
PRIDEP13838.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3750. rat.

Organism-specific databases

RGD3750. Spn.

Phylogenomic databases

eggNOGNOG47487.
HOGENOMHOG000294199.
HOVERGENHBG006258.
InParanoidP13838.
OrthoDBEOG7HXCVB.
PhylomeDBP13838.
TreeFamTF337688.

Gene expression databases

GenevestigatorP13838.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameLEUK_RAT
AccessionPrimary (citable) accession number: P13838
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program