P13810 (E2AA_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Heat-labile enterotoxin IIA, A chain Short name=LT-IIA
Organism	Escherichia coli
Taxonomic identifier	562 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
Subunit structure	Heterohexamer of one A chain and of five B chains.
Sequence similarities	Belongs to the enterotoxin A family.

Ontologies

Keywords
Domain	Signal
Molecular function	Enterotoxin Toxin
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 259	241	Heat-labile enterotoxin IIA, A chain		PRO_0000019353
Regions
	Nucleotide binding	23 – 37	15	NAD By similarity
Sites
	Active site	128	1	By similarity
Amino acid modifications
	Disulfide bond	203 ↔ 215		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13810 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 996F311A32CABEAA	FASTA	259	29,242
	10 20 30 40 50 60 MIKHVLLFFV FISFSVSAND FFRADSRTPD EIRRAGGLLP RGQQEAYERG TPININLYEH 70 80 90 100 110 120 ARGTVTGNTR YNDGYVSTTV TLRQAHLIGQ NILGSYNEYY IYVVAPAPNL FDVNGVLGRY 130 140 150 160 170 180 SPYPSENEFA ALGGIPLSQI IGWYRVSFGA IEGGMQRNRD YRGDLFRGLT VAPNEDGYQL 190 200 210 220 230 240 AGFPSNFPAW REMPWSTFAP EQCVPNNKEF KGGVCISATN VLSKYDLMNF KKLLKRRLAL 250 TFFMSEDDFI GVHGERDEL

References

[1]

"Genetics of type IIa heat-labile enterotoxin of Escherichia coli: operon fusions, nucleotide sequence, and hybridization studies." Pickett C.L., Weinstein D.L., Holmes R.K. J. Bacteriol. 169:5180-5187(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SA53.

Cross-references

Sequence databases
EMBL GenBank DDBJ	JQ031711 Genomic DNA. Translation: AAA24093.1.
PIR	A29831.
3D structure databases
ProteinModelPortal	P13810.
SMR	P13810. Positions 19-205.
ModBase	Search...
Proteomic databases
PRIDE	P13810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001144. Enterotoxin_A. [Graphical view]
Pfam	PF01375. Enterotoxin_a. 1 hit. [Graphical view]
PRINTS	PR00771. ENTEROTOXINA.
ProtoNet	Search...

Entry information

Entry name

E2AA_ECOLX

Accession

Primary (citable) accession number: P13810

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program