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Protein

Electron transfer flavoprotein subunit alpha, mitochondrial

Gene

Etfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Cofactori

FADNote: Binds 1 FAD per dimer.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi274 – 30229FADSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-RNO-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein subunit alpha, mitochondrial
Short name:
Alpha-ETF
Gene namesi
Name:Etfa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi628747. Etfa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1919MitochondrionSequence analysisAdd
BLAST
Chaini20 – 333314Electron transfer flavoprotein subunit alpha, mitochondrialPRO_0000008654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 591N6-acetyllysine; alternateBy similarity
Modified residuei59 – 591N6-succinyllysine; alternateBy similarity
Modified residuei62 – 621N6-acetyllysineBy similarity
Modified residuei69 – 691N6-acetyllysine; alternateBy similarity
Modified residuei69 – 691N6-succinyllysine; alternateBy similarity
Modified residuei75 – 751N6-acetyllysineBy similarity
Modified residuei85 – 851N6-acetyllysine; alternateBy similarity
Modified residuei85 – 851N6-succinyllysine; alternateBy similarity
Modified residuei93 – 931PhosphothreonineBy similarity
Modified residuei101 – 1011N6-acetyllysineBy similarity
Modified residuei139 – 1391N6-acetyllysineBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei158 – 1581N6-acetyllysine; alternateBy similarity
Modified residuei158 – 1581N6-succinyllysine; alternateBy similarity
Modified residuei164 – 1641N6-acetyllysineBy similarity
Modified residuei187 – 1871N6-succinyllysineBy similarity
Modified residuei203 – 2031N6-acetyllysine; alternateBy similarity
Modified residuei203 – 2031N6-succinyllysine; alternateBy similarity
Modified residuei216 – 2161N6-succinyllysineBy similarity
Modified residuei226 – 2261N6-acetyllysine; alternateBy similarity
Modified residuei226 – 2261N6-succinyllysine; alternateBy similarity
Modified residuei232 – 2321N6-acetyllysine; alternateBy similarity
Modified residuei232 – 2321N6-succinyllysine; alternateBy similarity
Modified residuei301 – 3011N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP13803.
PRIDEiP13803.

PTM databases

iPTMnetiP13803.

Expressioni

Gene expression databases

GenevisibleiP13803. RN.

Interactioni

Subunit structurei

Electron transfer flavoprotein is a heterodimer composed of ETFA and ETFB.By similarity

Protein-protein interaction databases

IntActiP13803. 1 interaction.
STRINGi10116.ENSRNOP00000020544.

Structurei

3D structure databases

ProteinModelPortaliP13803.
SMRiP13803. Positions 19-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 204185Domain IBy similarityAdd
BLAST
Regioni205 – 333129Domain IIBy similarityAdd
BLAST

Domaini

Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.By similarity

Sequence similaritiesi

Belongs to the ETF alpha-subunit/FixB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3954. Eukaryota.
COG2025. LUCA.
GeneTreeiENSGT00390000013422.
HOGENOMiHOG000247865.
HOVERGENiHBG002317.
InParanoidiP13803.
KOiK03522.
OMAiCCIESID.
OrthoDBiEOG76MK8R.
PhylomeDBiP13803.
TreeFamiTF105763.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR014730. ETF_a/b_N.
IPR001308. ETF_a/FixB.
IPR014731. ETF_asu_C.
IPR018206. ETF_asu_C_CS.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01012. ETF. 1 hit.
PF00766. ETF_alpha. 1 hit.
[Graphical view]
PIRSFiPIRSF000089. Electra_flavoP_a. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS00696. ETF_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRAAAPGQL RRAASLLRFQ STLVIAEHAN DSLAPITLNT ITAAGRLGGE
60 70 80 90 100
VSCLVAGTKC DKVVQDLCKV AGVAKVLVAQ HDAYKGLLPE ELTPLILETQ
110 120 130 140 150
KQFSYTHICA GASAFGKNLL PRVAAKLNVA PVSDIIEIKS PDTFVRTIYA
160 170 180 190 200
GNALCTVKCD EKVKVFSVRG TSFEAAAASG GSASSEKAPS SSSAGISEWL
210 220 230 240 250
DQKLTKSDRP ELTGAKVVVS GGRGLKSGEN FKLLYDLADQ LHAAVGASRA
260 270 280 290 300
AVDAGFVPND MQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKTIVAIN
310 320 330
KDPEAPIFQV ADYGIVADLF KVVPEMTEIL KKK
Length:333
Mass (Da):34,951
Last modified:January 9, 2007 - v4
Checksum:i635A3525666AFE85
GO

Sequence cautioni

The sequence AAA41130.1 differs from that shown. Reason: Frameshift at position 290. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091C → V in AAA41130 (PubMed:3415685).Curated
Sequence conflicti151 – 1511G → A in AAA41130 (PubMed:3415685).Curated
Sequence conflicti234 – 2341L → Q in AAA41130 (PubMed:3415685).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22030 Genomic DNA. Translation: AAA41130.1. Frameshift.
BC088412 mRNA. Translation: AAH88412.1.
PIRiA31568.
RefSeqiNP_001009668.1. NM_001009668.1.
UniGeneiRn.32496.

Genome annotation databases

EnsembliENSRNOT00000020544; ENSRNOP00000020544; ENSRNOG00000015233.
GeneIDi300726.
KEGGirno:300726.
UCSCiRGD:628747. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22030 Genomic DNA. Translation: AAA41130.1. Frameshift.
BC088412 mRNA. Translation: AAH88412.1.
PIRiA31568.
RefSeqiNP_001009668.1. NM_001009668.1.
UniGeneiRn.32496.

3D structure databases

ProteinModelPortaliP13803.
SMRiP13803. Positions 19-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13803. 1 interaction.
STRINGi10116.ENSRNOP00000020544.

PTM databases

iPTMnetiP13803.

Proteomic databases

PaxDbiP13803.
PRIDEiP13803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020544; ENSRNOP00000020544; ENSRNOG00000015233.
GeneIDi300726.
KEGGirno:300726.
UCSCiRGD:628747. rat.

Organism-specific databases

CTDi2108.
RGDi628747. Etfa.

Phylogenomic databases

eggNOGiKOG3954. Eukaryota.
COG2025. LUCA.
GeneTreeiENSGT00390000013422.
HOGENOMiHOG000247865.
HOVERGENiHBG002317.
InParanoidiP13803.
KOiK03522.
OMAiCCIESID.
OrthoDBiEOG76MK8R.
PhylomeDBiP13803.
TreeFamiTF105763.

Enzyme and pathway databases

ReactomeiR-RNO-611105. Respiratory electron transport.

Miscellaneous databases

PROiP13803.

Gene expression databases

GenevisibleiP13803. RN.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR014730. ETF_a/b_N.
IPR001308. ETF_a/FixB.
IPR014731. ETF_asu_C.
IPR018206. ETF_asu_C_CS.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01012. ETF. 1 hit.
PF00766. ETF_alpha. 1 hit.
[Graphical view]
PIRSFiPIRSF000089. Electra_flavoP_a. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS00696. ETF_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a cDNA for alpha-subunit of rat liver electron transfer flavoprotein."
    Shinzawa K., Inagaki T., Ohishi N., Ichihara C., Tsukagoshi N., Udaka S., Yagi K.
    Biochem. Biophys. Res. Commun. 155:300-304(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 86-117; 127-139 AND 233-249, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiETFA_RAT
AccessioniPrimary (citable) accession number: P13803
Secondary accession number(s): Q5M7W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 9, 2007
Last modified: July 6, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.