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Protein

Electron transfer flavoprotein subunit alpha, mitochondrial

Gene

Etfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:7334008). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.By similarityCurated1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223FADBy similarity1
Binding sitei248FADBy similarity1
Binding sitei300FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi263 – 266FADBy similarity4
Nucleotide bindingi281 – 286FADBy similarity6
Nucleotide bindingi318 – 319FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-RNO-611105 Respiratory electron transport

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein subunit alpha, mitochondrial
Short name:
Alpha-ETF
Gene namesi
Name:Etfa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi628747 Etfa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionSequence analysisAdd BLAST19
ChainiPRO_000000865420 – 333Electron transfer flavoprotein subunit alpha, mitochondrialAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59N6-acetyllysine; alternateBy similarity1
Modified residuei59N6-succinyllysine; alternateBy similarity1
Modified residuei62N6-acetyllysineBy similarity1
Modified residuei69N6-acetyllysine; alternateBy similarity1
Modified residuei69N6-succinyllysine; alternateBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei85N6-acetyllysine; alternateBy similarity1
Modified residuei85N6-succinyllysine; alternateBy similarity1
Modified residuei93PhosphothreonineBy similarity1
Modified residuei101N6-acetyllysineBy similarity1
Modified residuei139N6-acetyllysineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei158N6-acetyllysine; alternateBy similarity1
Modified residuei158N6-succinyllysine; alternateBy similarity1
Modified residuei164N6-acetyllysineBy similarity1
Modified residuei187N6-succinyllysineBy similarity1
Modified residuei203N6-acetyllysine; alternateBy similarity1
Modified residuei203N6-succinyllysine; alternateBy similarity1
Modified residuei216N6-succinyllysineBy similarity1
Modified residuei226N6-acetyllysine; alternateBy similarity1
Modified residuei226N6-succinyllysine; alternateBy similarity1
Modified residuei232N6-acetyllysine; alternateBy similarity1
Modified residuei232N6-succinyllysine; alternateBy similarity1
Modified residuei301N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP13803
PRIDEiP13803

PTM databases

iPTMnetiP13803
PhosphoSitePlusiP13803

Expressioni

Gene expression databases

BgeeiENSRNOG00000015233
GenevisibleiP13803 RN

Interactioni

Subunit structurei

Heterodimer composed of ETFA and ETFB(PubMed:7334008). Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity (By similarity).By similarity1 Publication

Protein-protein interaction databases

IntActiP13803, 1 interactor
STRINGi10116.ENSRNOP00000020544

Structurei

3D structure databases

ProteinModelPortaliP13803
SMRiP13803
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 204Domain IBy similarityAdd BLAST185
Regioni205 – 333Domain IIBy similarityAdd BLAST129

Domaini

Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.By similarity

Sequence similaritiesi

Belongs to the ETF alpha-subunit/FixB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3954 Eukaryota
COG2025 LUCA
GeneTreeiENSGT00390000013422
HOGENOMiHOG000247865
HOVERGENiHBG002317
InParanoidiP13803
KOiK03522
OMAiSQFKFTH
OrthoDBiEOG091G0EQM
PhylomeDBiP13803
TreeFamiTF105763

Family and domain databases

CDDicd01715 ETF_alpha, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR014730 ETF_a/b_N
IPR001308 ETF_a/FixB
IPR033947 ETF_alpha_N
IPR014731 ETF_asu_C
IPR018206 ETF_asu_C_CS
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01012 ETF, 1 hit
PF00766 ETF_alpha, 1 hit
PIRSFiPIRSF000089 Electra_flavoP_a, 1 hit
SMARTiView protein in SMART
SM00893 ETF, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
PROSITEiView protein in PROSITE
PS00696 ETF_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRAAAPGQL RRAASLLRFQ STLVIAEHAN DSLAPITLNT ITAAGRLGGE
60 70 80 90 100
VSCLVAGTKC DKVVQDLCKV AGVAKVLVAQ HDAYKGLLPE ELTPLILETQ
110 120 130 140 150
KQFSYTHICA GASAFGKNLL PRVAAKLNVA PVSDIIEIKS PDTFVRTIYA
160 170 180 190 200
GNALCTVKCD EKVKVFSVRG TSFEAAAASG GSASSEKAPS SSSAGISEWL
210 220 230 240 250
DQKLTKSDRP ELTGAKVVVS GGRGLKSGEN FKLLYDLADQ LHAAVGASRA
260 270 280 290 300
AVDAGFVPND MQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKTIVAIN
310 320 330
KDPEAPIFQV ADYGIVADLF KVVPEMTEIL KKK
Length:333
Mass (Da):34,951
Last modified:January 9, 2007 - v4
Checksum:i635A3525666AFE85
GO

Sequence cautioni

The sequence AAA41130 differs from that shown. Reason: Frameshift at position 290.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109C → V in AAA41130 (PubMed:3415685).Curated1
Sequence conflicti151G → A in AAA41130 (PubMed:3415685).Curated1
Sequence conflicti234L → Q in AAA41130 (PubMed:3415685).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22030 Genomic DNA Translation: AAA41130.1 Frameshift.
BC088412 mRNA Translation: AAH88412.1
PIRiA31568
RefSeqiNP_001009668.1, NM_001009668.1
UniGeneiRn.32496

Genome annotation databases

EnsembliENSRNOT00000020544; ENSRNOP00000020544; ENSRNOG00000015233
GeneIDi300726
KEGGirno:300726
UCSCiRGD:628747 rat

Entry informationi

Entry nameiETFA_RAT
AccessioniPrimary (citable) accession number: P13803
Secondary accession number(s): Q5M7W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 143 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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