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Protein

Signal transduction histidine-protein kinase/phosphatase DegS

Gene

degS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase. Involved in the control of expression of different cellular functions, including production of degradative enzymes such as the neutral and alkaline proteases, flagellum formation and biofilm formation. Acts as both a protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to DegU, and a protein phosphatase that dephosphorylates phospho-DegU.6 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Regulated via serine phosphorylation of its input domain. Phosphotransfer from DegS to DegU is stimulated by phosphorylation on Ser-76 and by DegQ.2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU35500-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase/phosphatase DegS (EC:2.7.13.3, EC:3.1.3.-)
Gene namesi
Name:degS
Synonyms:sacU
Ordered Locus Names:BSU35500
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76S → A: Lack of phosphorylation by YbdM. 1 Publication1
Mutagenesisi76S → D: Shows increased phosphorylation, as well as increased autophosphorylation and phosphotransfer to DegU. Negatively affects competence development. 1 Publication1
Mutagenesisi193A → V: Retains its autophosphorylation activity, but cannot phosphorylate DegU. 1 Publication1
Mutagenesisi218G → E: Retains its autophosphorylation activity, but shows a decrease in DegU phosphorylation activity. Decreases the rate of dephosphorylation of DegU. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747561 – 385Signal transduction histidine-protein kinase/phosphatase DegSAdd BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76Phosphoserine2 Publications1
Modified residuei189Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated. Phosphorylated in vitro at Ser-76 by the serine/threonine-protein kinase YbdM, which stimulates the phosphate transfer to DegU.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP13799.
PRIDEiP13799.

PTM databases

iPTMnetiP13799.

Interactioni

Protein-protein interaction databases

IntActiP13799. 6 interactors.
STRINGi224308.Bsubs1_010100019201.

Structurei

3D structure databases

ProteinModelPortaliP13799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini183 – 385Histidine kinasePROSITE-ProRule annotationAdd BLAST203

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili31 – 141Sequence analysisAdd BLAST111

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000235655.
InParanoidiP13799.
KOiK07777.
OMAiPAQMMAN.
PhylomeDBiP13799.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR008595. DegS.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR016381. Sig_transdc_His_kinase_DegS.
[Graphical view]
PfamiPF05384. DegS. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF003169. STHK_DegS. 1 hit.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTKMDSKV LDSILMKMLK TVDGSKDEVF QIGEQSRQQY EQLVEELKQI
60 70 80 90 100
KQQVYEVIEL GDKLEVQTRH ARNRLSEVSR NFHRFSEEEI RNAYEKAHKL
110 120 130 140 150
QVELTMIQQR EKQLRERRDD LERRLLGLQE IIERSESLVS QITVVLNYLN
160 170 180 190 200
QDLREVGLLL ADAQAKQDFG LRIIEAQEEE RKRVSREIHD GPAQMLANVM
210 220 230 240 250
MRSELIERIF RDRGAEDGFQ EIKNLRQNVR NALYEVRRII YDLRPMALDD
260 270 280 290 300
LGLIPTLRKY LYTTEEYNGK VKIHFQCIGE TEDQRLAPQF EVALFRLAQE
310 320 330 340 350
AVSNALKHSE SEEITVKVEI TKDFVILMIK DNGKGFDLKE AKEKKNKSFG
360 370 380
LLGMKERVDL LEGTMTIDSK IGLGTFIMIK VPLSL
Length:385
Mass (Da):44,958
Last modified:December 1, 1992 - v2
Checksum:i871109565A7E78F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23558 Genomic DNA. Translation: AAA22732.1.
M23649 Genomic DNA. Translation: AAA22734.1.
U56901 Genomic DNA. Translation: AAC44937.1.
AL009126 Genomic DNA. Translation: CAB15567.1.
M21658 Genomic DNA. Translation: AAA22544.1.
PIRiB30191. RGBSDS.
RefSeqiNP_391430.1. NC_000964.3.
WP_003227983.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15567; CAB15567; BSU35500.
GeneIDi936752.
KEGGibsu:BSU35500.
PATRICi18979108. VBIBacSub10457_3716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23558 Genomic DNA. Translation: AAA22732.1.
M23649 Genomic DNA. Translation: AAA22734.1.
U56901 Genomic DNA. Translation: AAC44937.1.
AL009126 Genomic DNA. Translation: CAB15567.1.
M21658 Genomic DNA. Translation: AAA22544.1.
PIRiB30191. RGBSDS.
RefSeqiNP_391430.1. NC_000964.3.
WP_003227983.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliP13799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13799. 6 interactors.
STRINGi224308.Bsubs1_010100019201.

PTM databases

iPTMnetiP13799.

Proteomic databases

PaxDbiP13799.
PRIDEiP13799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15567; CAB15567; BSU35500.
GeneIDi936752.
KEGGibsu:BSU35500.
PATRICi18979108. VBIBacSub10457_3716.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000235655.
InParanoidiP13799.
KOiK07777.
OMAiPAQMMAN.
PhylomeDBiP13799.

Enzyme and pathway databases

BioCyciBSUB:BSU35500-MONOMER.
BRENDAi2.7.13.3. 658.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR008595. DegS.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR016381. Sig_transdc_His_kinase_DegS.
[Graphical view]
PfamiPF05384. DegS. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF003169. STHK_DegS. 1 hit.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEGS_BACSU
AccessioniPrimary (citable) accession number: P13799
Secondary accession number(s): P19590
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 1992
Last modified: October 5, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.