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Protein

Acylamino-acid-releasing enzyme

Gene

APEH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.2 Publications

Catalytic activityi

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei587 – 5871Charge relay systemPROSITE-ProRule annotation1 Publication
Active sitei675 – 6751Charge relay systemPROSITE-ProRule annotation
Active sitei707 – 7071Charge relay systemPROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • omega peptidase activity Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • beta-amyloid metabolic process Source: UniProtKB
  • proteolysis Source: UniProtKB
  • translational termination Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiR-HSA-72764. Eukaryotic Translation Termination.

Protein family/group databases

ESTHERihuman-APEH. ACPH_Peptidase_S9.
MEROPSiS09.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Acylamino-acid-releasing enzyme (EC:3.4.19.1)
Short name:
AARE
Alternative name(s):
Acyl-peptide hydrolase
Short name:
APH
Acylaminoacyl-peptidase
Oxidized protein hydrolase
Short name:
OPH
Gene namesi
Name:APEH
Synonyms:D3F15S2, D3S48E, DNF15S2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:586. APEH.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nuclear membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24878.

Chemistry

ChEMBLiCHEMBL1741174.
GuidetoPHARMACOLOGYi2328.

Polymorphism and mutation databases

BioMutaiAPEH.
DMDMi38258902.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Acylamino-acid-releasing enzymePRO_0000122430Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei185 – 1851PhosphoserineCombined sources
Modified residuei187 – 1871PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP13798.
MaxQBiP13798.
PaxDbiP13798.
PeptideAtlasiP13798.
PRIDEiP13798.

PTM databases

iPTMnetiP13798.
PhosphoSiteiP13798.

Expressioni

Gene expression databases

BgeeiENSG00000164062.
CleanExiHS_APEH.
ExpressionAtlasiP13798. baseline and differential.
GenevisibleiP13798. HS.

Organism-specific databases

HPAiHPA029700.
HPA029701.
HPA029702.
HPA029703.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
IST1P539904EBI-723792,EBI-945994
LGALS8O002143EBI-723792,EBI-740058

Protein-protein interaction databases

BioGridi106824. 39 interactions.
IntActiP13798. 4 interactions.
MINTiMINT-5004051.
STRINGi9606.ENSP00000296456.

Structurei

3D structure databases

ProteinModelPortaliP13798.
SMRiP13798. Positions 375-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9C family.Curated

Phylogenomic databases

eggNOGiKOG2100. Eukaryota.
COG1506. LUCA.
GeneTreeiENSGT00390000013172.
HOGENOMiHOG000007443.
HOVERGENiHBG000869.
InParanoidiP13798.
KOiK01303.
PhylomeDBiP13798.
TreeFamiTF312937.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
3.40.50.1820. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
[Graphical view]
PfamiPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERQVLLSEP EEAAALYRGL SRQPALSAAC LGPEVTTQYG GQYRTVHTEW
60 70 80 90 100
TQRDLERMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG
110 120 130 140 150
TMKAVLRKAG GTGPGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF
160 170 180 190 200
GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDVSASDDE IARLKKPDQA
210 220 230 240 250
IKGDQFVFYE DWGENMVSKS IPVLCVLDVE SGNISVLEGV PENVSPGQAF
260 270 280 290 300
WAPGDAGVVF VGWWHEPFRL GIRFCTNRRS ALYYVDLIGG KCELLSDDSL
310 320 330 340 350
AVSSPRLSPD QCRIVYLQYP SLIPHHQCSQ LCLYDWYTKV TSVVVDVVPR
360 370 380 390 400
QLGENFSGIY CSLLPLGCWS ADSQRVVFDS AQRSRQDLFA VDTQVGTVTS
410 420 430 440 450
LTAGGSGGSW KLLTIDQDLM VAQFSTPSLP PTLKVGFLPS AGKEQSVLWV
460 470 480 490 500
SLEEAEPIPD IHWGIRVLQP PPEQENVQYA GLDFEAILLQ PGSPPDKTQV
510 520 530 540 550
PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS TGFGQDSILS
560 570 580 590 600
LPGNVGHQDV KDVQFAVEQV LQEEHFDASH VALMGGSHGG FISCHLIGQY
610 620 630 640 650
PETYRACVAR NPVINIASML GSTDIPDWCV VEAGFPFSSD CLPDLSVWAE
660 670 680 690 700
MLDKSPIRYI PQVKTPLLLM LGQEDRRVPF KQGMEYYRAL KTRNVPVRLL
710 720 730
LYPKSTHALS EVEVESDSFM NAVLWLRTHL GS
Length:732
Mass (Da):81,225
Last modified:November 7, 2003 - v4
Checksum:iA2C370516324D851
GO

Sequence cautioni

The sequence AAA35769 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011T → S in BAA07476 (PubMed:8724851).Curated
Sequence conflicti137 – 1371A → V in BAA07476 (PubMed:8724851).Curated
Sequence conflicti168 – 1681K → R in BAA07476 (PubMed:8724851).Curated
Sequence conflicti200 – 2001A → P in BAA07476 (PubMed:8724851).Curated
Sequence conflicti403 – 4031A → V in AAF37321 (PubMed:10719179).Curated

Mass spectrometryi

Molecular mass is 81269.9±8.7 Da from positions 1 - 732. Determined by ESI. 1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti541 – 5411T → M.
Corresponds to variant rs3816877 [ dbSNP | Ensembl ].
VAR_051580

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38441 mRNA. Translation: BAA07476.1.
AF141383 mRNA. Translation: AAF37321.1.
BC000362 mRNA. Translation: AAH00362.1.
BC001499 mRNA. Translation: AAH01499.1.
BC001826 mRNA. Translation: AAH01826.1.
J03068 mRNA. Translation: AAA35769.1. Frameshift.
CCDSiCCDS2801.1.
PIRiJC4655.
RefSeqiNP_001631.3. NM_001640.3.
XP_011531962.1. XM_011533660.2.
UniGeneiHs.517969.

Genome annotation databases

EnsembliENST00000296456; ENSP00000296456; ENSG00000164062.
GeneIDi327.
KEGGihsa:327.
UCSCiuc003cxf.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38441 mRNA. Translation: BAA07476.1.
AF141383 mRNA. Translation: AAF37321.1.
BC000362 mRNA. Translation: AAH00362.1.
BC001499 mRNA. Translation: AAH01499.1.
BC001826 mRNA. Translation: AAH01826.1.
J03068 mRNA. Translation: AAA35769.1. Frameshift.
CCDSiCCDS2801.1.
PIRiJC4655.
RefSeqiNP_001631.3. NM_001640.3.
XP_011531962.1. XM_011533660.2.
UniGeneiHs.517969.

3D structure databases

ProteinModelPortaliP13798.
SMRiP13798. Positions 375-729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106824. 39 interactions.
IntActiP13798. 4 interactions.
MINTiMINT-5004051.
STRINGi9606.ENSP00000296456.

Chemistry

ChEMBLiCHEMBL1741174.
GuidetoPHARMACOLOGYi2328.

Protein family/group databases

ESTHERihuman-APEH. ACPH_Peptidase_S9.
MEROPSiS09.004.

PTM databases

iPTMnetiP13798.
PhosphoSiteiP13798.

Polymorphism and mutation databases

BioMutaiAPEH.
DMDMi38258902.

Proteomic databases

EPDiP13798.
MaxQBiP13798.
PaxDbiP13798.
PeptideAtlasiP13798.
PRIDEiP13798.

Protocols and materials databases

DNASUi327.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296456; ENSP00000296456; ENSG00000164062.
GeneIDi327.
KEGGihsa:327.
UCSCiuc003cxf.4. human.

Organism-specific databases

CTDi327.
GeneCardsiAPEH.
HGNCiHGNC:586. APEH.
HPAiHPA029700.
HPA029701.
HPA029702.
HPA029703.
MIMi102645. gene.
neXtProtiNX_P13798.
PharmGKBiPA24878.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2100. Eukaryota.
COG1506. LUCA.
GeneTreeiENSGT00390000013172.
HOGENOMiHOG000007443.
HOVERGENiHBG000869.
InParanoidiP13798.
KOiK01303.
PhylomeDBiP13798.
TreeFamiTF312937.

Enzyme and pathway databases

ReactomeiR-HSA-72764. Eukaryotic Translation Termination.

Miscellaneous databases

ChiTaRSiAPEH. human.
GeneWikiiAPEH_(gene).
GenomeRNAii327.
PROiP13798.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164062.
CleanExiHS_APEH.
ExpressionAtlasiP13798. baseline and differential.
GenevisibleiP13798. HS.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
3.40.50.1820. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
[Graphical view]
PfamiPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACPH_HUMAN
AccessioniPrimary (citable) accession number: P13798
Secondary accession number(s): Q9BQ33, Q9P0Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 7, 2003
Last modified: September 7, 2016
This is version 162 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.