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Protein

Outer membrane porin F

Gene

oprF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • complement component C3b binding Source: PseudoCAP
  • porin activity Source: PseudoCAP

GO - Biological processi

  • adhesion of symbiont to host Source: PseudoCAP
  • ion transport Source: UniProtKB-KW
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionPorin
Biological processCell shape, Ion transport, Transport

Protein family/group databases

TCDBi1.B.6.1.2. the ompa-ompf porin (oop) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane porin F
Gene namesi
Name:oprF
Ordered Locus Names:PA1777
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1777.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB-SubCell
  • outer membrane Source: PseudoCAP
  • pore complex Source: UniProtKB-KW

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000002011525 – 350Outer membrane porin FAdd BLAST326

Post-translational modificationi

Contains two disulfide bonds.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP13794.
PRIDEiP13794.

Interactioni

GO - Molecular functioni

  • complement component C3b binding Source: PseudoCAP

Protein-protein interaction databases

STRINGi208964.PA1777.

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 39Combined sources10
Beta strandi51 – 75Combined sources25
Beta strandi89 – 100Combined sources12
Beta strandi104 – 124Combined sources21
Beta strandi129 – 162Combined sources34
Beta strandi173 – 184Combined sources12
Beta strandi231 – 243Combined sources13
Helixi253 – 255Combined sources3
Helixi256 – 268Combined sources13
Beta strandi274 – 278Combined sources5
Beta strandi282 – 284Combined sources3
Helixi286 – 306Combined sources21
Helixi312 – 314Combined sources3
Beta strandi315 – 319Combined sources5
Helixi332 – 338Combined sources7
Beta strandi339 – 348Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RLCX-ray1.60A25-184[»]
5U1HX-ray1.50A/B/C/D231-350[»]
ProteinModelPortaliP13794.
SMRiP13794.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati190 – 19112
Repeati192 – 19322
Repeati194 – 19532
Domaini231 – 349OmpA-likePROSITE-ProRule annotationAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 1953 X 2 AA tandem repeats of X-P6

Sequence similaritiesi

Belongs to the OmpA family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CDV. Bacteria.
COG2885. LUCA.
HOGENOMiHOG000295618.
InParanoidiP13794.
KOiK03286.
OMAiADFMQQY.
PhylomeDBiP13794.

Family and domain databases

CDDicd07185. OmpA_C-like. 1 hit.
Gene3Di2.40.160.20. 1 hit.
3.30.1330.60. 1 hit.
InterProiView protein in InterPro
IPR011250. OMP/PagP_b-brl.
IPR006664. OMP_bac.
IPR006665. OmpA-like.
IPR006690. OMPA-like_CS.
IPR008722. OprF_membrane_N.
IPR028974. TSP_type-3_rpt.
PfamiView protein in Pfam
PF00691. OmpA. 1 hit.
PF05736. OprF. 1 hit.
PRINTSiPR01021. OMPADOMAIN.
SUPFAMiSSF103088. SSF103088. 1 hit.
SSF103647. SSF103647. 1 hit.
SSF56925. SSF56925. 1 hit.
PROSITEiView protein in PROSITE
PS01068. OMPA_1. 1 hit.
PS51123. OMPA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKNTLGVV IGSLVAASAM NAFAQGQNSV EIEAFGKRYF TDSVRNMKNA
60 70 80 90 100
DLYGGSIGYF LTDDVELALS YGEYHDVRGT YETGNKKVHG NLTSLDAIYH
110 120 130 140 150
FGTPGVGLRP YVSAGLAHQN ITNINSDSQG RQQMTMANIG AGLKYYFTEN
160 170 180 190 200
FFAKASLDGQ YGLEKRDNGH QGEWMAGLGV GFNFGGSKAA PAPEPVADVC
210 220 230 240 250
SDSDNDGVCD NVDKCPDTPA NVTVDANGCP AVAEVVRVQL DVKFDFDKSK
260 270 280 290 300
VKENSYADIK NLADFMKQYP STSTTVEGHT DSVGTDAYNQ KLSERRANAV
310 320 330 340 350
RDVLVNEYGV EGGRVNAVGY GESRPVADNA TAEGRAINRR VEAEVEAEAK
Length:350
Mass (Da):37,640
Last modified:January 1, 1990 - v1
Checksum:iD1F758F076874383
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027290 Genomic DNA. Translation: AAD11568.1.
M18975 Genomic DNA. Translation: AAA25973.1.
AE004091 Genomic DNA. Translation: AAG05166.1.
PIRiS39156.
RefSeqiNP_250468.1. NC_002516.2.
WP_003087843.1. NZ_ASJY01000250.1.

Genome annotation databases

EnsemblBacteriaiAAG05166; AAG05166; PA1777.
GeneIDi878442.
KEGGipae:PA1777.
PATRICi19837913. VBIPseAer58763_1842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027290 Genomic DNA. Translation: AAD11568.1.
M18975 Genomic DNA. Translation: AAA25973.1.
AE004091 Genomic DNA. Translation: AAG05166.1.
PIRiS39156.
RefSeqiNP_250468.1. NC_002516.2.
WP_003087843.1. NZ_ASJY01000250.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RLCX-ray1.60A25-184[»]
5U1HX-ray1.50A/B/C/D231-350[»]
ProteinModelPortaliP13794.
SMRiP13794.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1777.

Protein family/group databases

TCDBi1.B.6.1.2. the ompa-ompf porin (oop) family.

Proteomic databases

PaxDbiP13794.
PRIDEiP13794.

Protocols and materials databases

DNASUi878442.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG05166; AAG05166; PA1777.
GeneIDi878442.
KEGGipae:PA1777.
PATRICi19837913. VBIPseAer58763_1842.

Organism-specific databases

PseudoCAPiPA1777.

Phylogenomic databases

eggNOGiENOG4105CDV. Bacteria.
COG2885. LUCA.
HOGENOMiHOG000295618.
InParanoidiP13794.
KOiK03286.
OMAiADFMQQY.
PhylomeDBiP13794.

Family and domain databases

CDDicd07185. OmpA_C-like. 1 hit.
Gene3Di2.40.160.20. 1 hit.
3.30.1330.60. 1 hit.
InterProiView protein in InterPro
IPR011250. OMP/PagP_b-brl.
IPR006664. OMP_bac.
IPR006665. OmpA-like.
IPR006690. OMPA-like_CS.
IPR008722. OprF_membrane_N.
IPR028974. TSP_type-3_rpt.
PfamiView protein in Pfam
PF00691. OmpA. 1 hit.
PF05736. OprF. 1 hit.
PRINTSiPR01021. OMPADOMAIN.
SUPFAMiSSF103088. SSF103088. 1 hit.
SSF103647. SSF103647. 1 hit.
SSF56925. SSF56925. 1 hit.
PROSITEiView protein in PROSITE
PS01068. OMPA_1. 1 hit.
PS51123. OMPA_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPORF_PSEAE
AccessioniPrimary (citable) accession number: P13794
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 12, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.