ID   PHOP_BACSU              Reviewed;         240 AA.
AC   P13792; O34804;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 4.
DT   27-MAR-2024, entry version 172.
DE   RecName: Full=Alkaline phosphatase synthesis transcriptional regulatory protein PhoP;
GN   Name=phoP; OrderedLocusNames=BSU29110;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=1454550; DOI=10.1093/nar/20.21.5848;
RA   Lee J.W., Hulett F.M.;
RT   "Nucleotide sequence of the phoP gene encoding PhoP, the response regulator
RT   of the phosphate regulon of Bacillus subtilis.";
RL   Nucleic Acids Res. 20:5848-5848(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3036763; DOI=10.1128/jb.169.7.2913-2916.1987;
RA   Seki T., Yoshikawa H., Takahashi H., Saito H.;
RT   "Cloning and nucleotide sequence of phoP, the regulatory gene for alkaline
RT   phosphatase and phosphodiesterase in Bacillus subtilis.";
RL   J. Bacteriol. 169:2913-2916(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   SEQUENCE REVISION TO 124.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
RC   STRAIN=168 / SMY;
RX   PubMed=8045898; DOI=10.1128/jb.176.15.4669-4679.1994;
RA   Jin S., Sonenshein A.L.;
RT   "Identification of two distinct Bacillus subtilis citrate synthase genes.";
RL   J. Bacteriol. 176:4669-4679(1994).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 212-240.
RX   PubMed=3142862; DOI=10.1128/jb.170.12.5935-5938.1988;
RA   Seki T., Yoshikawa H., Takahashi H., Saito H.;
RT   "Nucleotide sequence of the Bacillus subtilis phoR gene.";
RL   J. Bacteriol. 170:5935-5938(1988).
CC   -!- FUNCTION: Member of the two-component regulatory system PhoP/PhoR
CC       involved in the regulation of alkaline phosphatase genes phoA and phoB
CC       and of phosphodiesterase.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by PhoR. {ECO:0000305}.
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DR   EMBL; X67676; CAA47908.1; -; Genomic_DNA.
DR   EMBL; M16775; AAA22661.1; -; Genomic_DNA.
DR   EMBL; AF008220; AAC00348.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14871.2; -; Genomic_DNA.
DR   EMBL; U05257; AAA96344.1; -; Genomic_DNA.
DR   EMBL; M23549; AAA22662.1; -; Genomic_DNA.
DR   PIR; F69676; RGBSAP.
DR   RefSeq; NP_390789.2; NC_000964.3.
DR   RefSeq; WP_003229442.1; NZ_JNCM01000036.1.
DR   PDB; 1MVO; X-ray; 1.60 A; A=1-135.
DR   PDBsum; 1MVO; -.
DR   AlphaFoldDB; P13792; -.
DR   SMR; P13792; -.
DR   STRING; 224308.BSU29110; -.
DR   jPOST; P13792; -.
DR   PaxDb; 224308-BSU29110; -.
DR   EnsemblBacteria; CAB14871; CAB14871; BSU_29110.
DR   GeneID; 936644; -.
DR   KEGG; bsu:BSU29110; -.
DR   PATRIC; fig|224308.179.peg.3160; -.
DR   eggNOG; COG0745; Bacteria.
DR   InParanoid; P13792; -.
DR   OrthoDB; 9790442at2; -.
DR   PhylomeDB; P13792; -.
DR   BioCyc; BSUB:BSU29110-MONOMER; -.
DR   EvolutionaryTrace; P13792; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   CDD; cd19937; REC_OmpR_BsPhoP-like; 1.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 3.40.50.2300; -; 1.
DR   Gene3D; 6.10.250.690; -; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; REGULATOR OF RPOS; 1.
DR   PANTHER; PTHR48111:SF40; SENSORY TRANSDUCTION PROTEIN REGX3; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; C-terminal effector domain of the bipartite response regulators; 1.
DR   SUPFAM; SSF52172; CheY-like; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cytoplasm; DNA-binding; Phosphate transport;
KW   Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Transport; Two-component regulatory system.
FT   CHAIN           1..240
FT                   /note="Alkaline phosphatase synthesis transcriptional
FT                   regulatory protein PhoP"
FT                   /id="PRO_0000081194"
FT   DOMAIN          4..118
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        136..235
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   CONFLICT        124
FT                   /note="A -> R (in Ref. 2; AAA22661 and 3; AAC00348)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        154
FT                   /note="Y -> I (in Ref. 2; AAA22661)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        211
FT                   /note="D -> PT (in Ref. 2; AAA22661)"
FT                   /evidence="ECO:0000305"
FT   STRAND          4..8
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   HELIX           12..24
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   STRAND          28..34
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   HELIX           35..45
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   STRAND          48..55
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   STRAND          57..59
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   HELIX           61..70
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   STRAND          77..81
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   HELIX           89..94
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   STRAND          99..104
FT                   /evidence="ECO:0007829|PDB:1MVO"
FT   HELIX           107..119
FT                   /evidence="ECO:0007829|PDB:1MVO"
SQ   SEQUENCE   240 AA;  27598 MW;  E61A6FADBE513D97 CRC64;
     MNKKILVVDD EESIVTLLQY NLERSGYDVI TASDGEEALK KAETEKPDLI VLDVMLPKLD
     GIEVCKQLRQ QKLMFPILML TAKDEEFDKV LGLELGADDY MTKPFSPREV NARVKAILRR
     SEIAAPSSEM KNDEMEGQIV IGDLKILPDH YEAYFKESQL ELTPKEFELL LYLGRHKGRV
     LTRDLLLSAV WNYDFAGDTR IVDVHISHLR DKIENNTKKP IYIKTIRGLG YKLEEPKMNE
//