P13792 (PHOP_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline phosphatase synthesis transcriptional regulatory protein PhoP

Gene names

Name:	phoP
Ordered Locus Names:	BSU29110

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system PhoP/PhoR involved in the regulation of alkaline phosphatase genes phoA and phoB and of phosphodiesterase.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by PhoR Probable.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Phosphate transport Transcription Transcription regulation Transport Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC phosphate ion transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

Alkaline phosphatase synthesis transcriptional regulatory protein PhoP

PRO_0000081194

Regions

Domain

4 – 118

115

Response regulatory

Amino acid modifications

Modified residue

53

1

4-aspartylphosphate By similarity

Experimental info

Sequence conflict

124

1

A → R in AAA22661. Ref.2

Sequence conflict

124

1

A → R in AAC00348. Ref.3

Sequence conflict

154

1

Y → I in AAA22661. Ref.2

Sequence conflict

211

1

D → PT in AAA22661. Ref.2

Secondary structure

1

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240

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

12 – 24

13

Beta strand

28 – 34

7

Helix

35 – 45

11

Beta strand

48 – 55

8

Beta strand

57 – 59

3

Helix

61 – 70

10

Beta strand

77 – 81

5

Helix

89 – 94

6

Beta strand

99 – 104

6

Helix

107 – 119

13

Sequences

	Sequence		Length	Mass (Da)
	P13792 [UniParc]. Last modified July 7, 2009. Version 4. Checksum: E61A6FADBE513D97	FASTA	240	27,598
	10 20 30 40 50 60 MNKKILVVDD EESIVTLLQY NLERSGYDVI TASDGEEALK KAETEKPDLI VLDVMLPKLD 70 80 90 100 110 120 GIEVCKQLRQ QKLMFPILML TAKDEEFDKV LGLELGADDY MTKPFSPREV NARVKAILRR 130 140 150 160 170 180 SEIAAPSSEM KNDEMEGQIV IGDLKILPDH YEAYFKESQL ELTPKEFELL LYLGRHKGRV 190 200 210 220 230 240 LTRDLLLSAV WNYDFAGDTR IVDVHISHLR DKIENNTKKP IYIKTIRGLG YKLEEPKMNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the phoP gene encoding PhoP, the response regulator of the phosphate regulon of Bacillus subtilis." Lee J.W., Hulett F.M. Nucleic Acids Res. 20:5848-5848(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"Cloning and nucleotide sequence of phoP, the regulatory gene for alkaline phosphatase and phosphodiesterase in Bacillus subtilis." Seki T., Yoshikawa H., Takahashi H., Saito H. J. Bacteriol. 169:2913-2916(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 124.
[6]	"Identification of two distinct Bacillus subtilis citrate synthase genes." Jin S., Sonenshein A.L. J. Bacteriol. 176:4669-4679(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6. Strain: 168 / SMY.
[7]	"Nucleotide sequence of the Bacillus subtilis phoR gene." Seki T., Yoshikawa H., Takahashi H., Saito H. J. Bacteriol. 170:5935-5938(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 212-240.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X67676 Genomic DNA. Translation: CAA47908.1.
M16775 Genomic DNA. Translation: AAA22661.1.
AF008220 Genomic DNA. Translation: AAC00348.1.
AL009126 Genomic DNA. Translation: CAB14871.2.
U05257 Genomic DNA. Translation: AAA96344.1.
M23549 Genomic DNA. Translation: AAA22662.1.

PIR

RGBSAP. F69676.

RefSeq

NP_390789.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MVO	X-ray	1.60	A	1-135	[»]

ProteinModelPortal

P13792.

SMR

P13792. Positions 1-237.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU29110.

Proteomic databases

PaxDb

P13792.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14871; CAB14871; BSU29110.

GeneID

936644.

KEGG

bsu:BSU29110.

PATRIC

18977714. VBIBacSub10457_3045.

Organism-specific databases

GenoList

BSU29110. [Micado]

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034819.

KO

K07658.

ProtClustDB

CLSK2393393.

Enzyme and pathway databases

BioCyc

BSUB:BSU29110-MONOMER.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P13792.

Entry information

Entry name

PHOP_BACSU

Accession

Primary (citable) accession number: P13792
Secondary accession number(s): O34804

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 122 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program