Cyclic AMP receptor 1 - Dictyostelium discoideum (Slime mold)

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P13773 (CAR1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclic AMP receptor 1
Short name=cAMP receptor 1

Gene names

Name:	carA-1
Synonyms:	car1
ORF Names:	DDB_G0273397
AND
Name:	carA-2
Synonyms:	car1
ORF Names:	DDB_G0273533

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for cAMP. Coordinates the aggregation of individual cells into a multicellular organism and regulates the expression of a large number of developmentally regulated genes. The activity of this receptor is mediated by G proteins. Ref.1 Ref.4 Ref.6 Ref.8 Ref.14 Ref.15 Ref.17 Ref.19
Subcellular location	Membrane; Multi-pass membrane protein Ref.11 Ref.12.
Developmental stage	Expressed predominantly during early aggregation and at low levels during later stage. Ref.4 Ref.5 Ref.6 Ref.9 Ref.18
Post-translational modification	C-terminal Ser or Thr residues may be phosphorylated. Ref.10 Ref.13 Ref.16
Sequence similarities	Belongs to the G-protein coupled receptor 5 family.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of Rap GTPase activity Inferred from mutant phenotype PubMed 21103944. Source: dictyBase activation of adenylate cyclase activity Inferred from genetic interaction Ref.15. Source: dictyBase activation of protein kinase B activity Inferred from mutant phenotype Ref.25. Source: dictyBase aggregation involved in sorocarp development Inferred from mutant phenotype Ref.6. Source: UniProtKB chemotaxis Inferred from mutant phenotype PubMed 15507682. Source: dictyBase desensitization of G-protein coupled receptor protein signaling pathway Inferred from mutant phenotype Ref.24. Source: UniProtKB regulation of phagocytosis Inferred from mutant phenotype PubMed 17905919. Source: dictyBase
Cellular_component	cell surface Inferred from direct assay PubMed 15507682. Source: dictyBase integral to plasma membrane Traceable author statement PubMed 15473840. Source: dictyBase
Molecular_function	G-protein coupled chemoattractant receptor activity Non-traceable author statement PubMed 9670017. Source: dictyBase cAMP binding Inferred from direct assay PubMed 15507682. Source: dictyBase cAMP receptor activity Inferred from direct assay Ref.15. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 392

392

Cyclic AMP receptor 1

PRO_0000195080

Regions

Topological domain

1 – 13

Extracellular Potential

Transmembrane

14 – 33

Helical; Name=1; Potential

Topological domain

34 – 47

Cytoplasmic Potential

Transmembrane

48 – 68

Helical; Name=2; Potential

Topological domain

69 – 83

Extracellular Potential

Transmembrane

84 – 109

Helical; Name=3; Potential

Topological domain

110 – 120

Cytoplasmic Potential

Transmembrane

121 – 139

Helical; Name=4; Potential

Topological domain

140 – 162

Extracellular Potential

Transmembrane

163 – 181

Helical; Name=5; Potential

Topological domain

182 – 205

Cytoplasmic Potential

Transmembrane

206 – 224

Helical; Name=6; Potential

Topological domain

225 – 235

Extracellular Potential

Transmembrane

236 – 260

Helical; Name=7; Potential

Topological domain

261 – 392

132

Cytoplasmic Potential

Compositional bias

337 – 356

Asn-rich

Compositional bias

360 – 368

Ser-rich

Amino acid modifications

Modified residue

299

Phosphoserine Potential

Modified residue

302

Phosphoserine Ref.13

Modified residue

303

Phosphoserine Ref.13

Modified residue

304

Phosphoserine Ref.13

Modified residue

308

Phosphoserine Potential

Modified residue

360

Phosphoserine Potential

Modified residue

361

Phosphoserine Potential

Modified residue

362

Phosphoserine Potential

Modified residue

363

Phosphoserine Potential

Modified residue

364

Phosphoserine Potential

Modified residue

366

Phosphoserine Potential

Modified residue

367

Phosphoserine Potential

Modified residue

368

Phosphoserine Potential

Glycosylation

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

100

L → H in DN3; constitutive phosphorylation and greatly increased affinity for cAMP. Ref.24

Mutagenesis

102

I → T in DN2; constitutive phosphorylation and greatly increased affinity for cAMP; when associated with D-225. Ref.24

Mutagenesis

104

I → N in DN4; constitutive phosphorylation and greatly increased affinity for cAMP. In DN1; Constitutive phosphorylation and greatly increased affinity for cAMP; when associated with A-136. Ref.24 Ref.25

Mutagenesis

104

I → X: Most substitutions increase affinity for cAMP and the most polar substitutions block development. Ref.24 Ref.25

Mutagenesis

136

V → A in DN1; constitutive phosphorylation and greatly increased affinity for cAMP; when associated with N-104. Ref.24

Mutagenesis

148

N → G: Reduces the affinity for cAMP by 100 to 1000-fold when associated with D-154; N-155; Y-156 and D-157. Ref.15 Ref.24

Mutagenesis

154

V → D: Reduces the affinity for cAMP by 100 to 1000-fold when associated with G-148; N-155; Y-156 and D-157. Ref.15 Ref.24

Mutagenesis

155

S → N: Reduces the affinity for cAMP by 100 to 1000-fold when associated with G-148; D-154; Y-156 and D-157. Ref.15 Ref.24

Mutagenesis

156

F → Y: Reduces the affinity for cAMP by 100 to 1000-fold when associated with G-148; D-154; N-155 and D-157. Ref.15 Ref.24

Mutagenesis

157

T → D: Reduces the affinity for cAMP by 100 to 1000-fold when associated with G-148; D-154; N-155 and Y-156. Ref.15 Ref.24

Mutagenesis

225

N → D in DN2; constitutive phosphorylation and greatly increased affinity for cAMP; when associated with T-102. Ref.24

Mutagenesis

288 – 392

105

Missing: No effect on cAMP-dependent gene expression, chemotaxis, aggregation and differentiation. Ref.14

Mutagenesis

299

S → A: Greatly reduces ligand induced phosphorylation; when associated with G-302; A-303; G-304 and A-308. Ref.10 Ref.13

Mutagenesis

302

S → G: Greatly reduces ligand induced phosphorylation; when associated with A-299; A-303; G-304 and A-308. Ref.10 Ref.13

Mutagenesis

303

S → A: Greatly reduces ligand induced phosphorylation; when associated with A-299; G-302; G-304 and A-308. Ref.10 Ref.13

Mutagenesis

304

S → G: Greatly reduces ligand induced phosphorylation; when associated with A-299; G-302; A-303 and A-308. Ref.10 Ref.13

Mutagenesis

308

S → A: Greatly reduces ligand induced phosphorylation; when associated with A-299; G-302; A-303 and G-304. Ref.10

Mutagenesis

324

S → G: Slightly reduces ligand induced and basal phosphorylation; when associated with A-325 and A-331. Ref.10

Mutagenesis

325

S → A: Slightly reduces ligand induced and basal phosphorylation; when associated with G-324 and A-331. Ref.10

Mutagenesis

331

S → A: Slightly reduces ligand induced and basal phosphorylation; when associated with G-324 and A-325. Ref.10

Mutagenesis

360 – 368

Missing: Greatly reduces basal phosphorylation. Ref.10

Mutagenesis

383

S → A: No effect on phosphorylation. Ref.10

Mutagenesis

385

S → A: No effect on phosphorylation. Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P13773 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 38C4561EE1CC9A69
Show »

FASTA

392

44,312

        10         20         30         40         50         60 
MGLLDGNPAN ETSLVLLLFA DFSSMLGCMA VLIGFWRLKL LRNHVTKVIA CFCATSFCKD 

        70         80         90        100        110        120 
FPSTILTLTN TAVNGGFPCY LYAIVITYGS FACWLWTLCL AISIYMLIVK REPEPERFEK 

       130        140        150        160        170        180 
YYYLLCWGLP LISTIVMLAK NTVQFVGNWC WIGVSFTGYR FGLFYGPFLF IWAISAVLVG 

       190        200        210        220        230        240 
LTSRYTYVVI HNGVSDNKEK HLTYQFKLIN YIIVFLVCWV FAVVNRIVNG LNMFPPALNI 

       250        260        270        280        290        300 
LHTYLSVSHG FWASVTFIYN NPLMWRYFGA KILTVFTFFG YFTDVQKKLE KNKNNNNPSP 

       310        320        330        340        350        360 
YSSSRGTSGK TMGGHPTGDD VQCSSDMEQC SLERHPNMVN NQQNLNNNYG LQQNYNDEGS 

       370        380        390 
SSSSLSSSDE EKQTVEMQNI QISTSTNGQG NN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A chemoattractant receptor controls development in Dictyostelium discoideum." Klein P.S., Sun T.J., Saxe C.L. III, Kimmel A.R., Johnson R.L., Devreotes P.N. Science 241:1467-1472(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Surface cAMP receptors mediate multiple responses during development in Dictyostelium: evidenced by antisense mutagenesis." Sun T.J., Van Haastert P.J.M., Devreotes P.N. J. Cell Biol. 110:1549-1554(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[5]	"Expression of a cAMP receptor gene of Dictyostelium and evidence for a multigene family." Saxe C.L. III, Johnson R.L., Devreotes P.N., Kimmel A.R. Genes Dev. 5:1-8(1991) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[6]	"Gene targeting of the aggregation stage cAMP receptor cAR1 in Dictyostelium." Sun T.J., Devreotes P.N. Genes Dev. 5:572-582(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[7]	"Two transmembrane signaling mechanisms control expression of the cAMP receptor gene CAR1 during Dictyostelium development." Louis J.M., Saxe C.L. III, Kimmel A.R. Proc. Natl. Acad. Sci. U.S.A. 90:5969-5973(1993) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"Extracellular cAMP can restore development in Dictyostelium cells lacking one, but not two subtypes of early cAMP receptors (cARs). Evidence for involvement of cAR1 in aggregative gene expression." Soede R.D.M., Insall R.H., Devreotes P.N., Schaap P. Development 120:1997-2002(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Precise expression of the cAMP receptor gene, CAR1, during transition from growth to differentiation in Dictyostelium discoideum." Abe F., Maeda Y. FEBS Lett. 342:239-241(1994) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[10]	"Localization of ligand-induced phosphorylation sites to serine clusters in the C-terminal domain of the Dictyostelium cAMP receptor, cAR1." Hereld D., Vaughan R., Kim J.-Y., Borleis J.A., Devreotes P.N. J. Biol. Chem. 269:7036-7044(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, MUTAGENESIS OF SER-299; SER-302; SER-303; SER-304; SER-308; SER-324; SER-325; SER-331; 360-SER--SER-368; SER-383 AND SER-385.
[11]	"Occupancy of the Dictyostelium cAMP receptor, cAR1, induces a reduction in affinity which depends upon COOH-terminal serine residues." Caterina M.J., Hereld D., Devreotes P.N. J. Biol. Chem. 270:4418-4423(1995) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[12]	"Dynamic distribution of chemoattractant receptors in living cells during chemotaxis and persistent stimulation." Xiao Z., Zhang N., Murphy D.B., Devreotes P.N. J. Cell Biol. 139:365-374(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[13]	"Agonist-induced loss of ligand binding is correlated with phosphorylation of cAR1, a G protein-coupled chemoattractant receptor from Dictyostelium." Caterina M.J., Devreotes P.N., Borleis J.A., Hereld D. J. Biol. Chem. 270:8667-8672(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-302; SER-303 AND SER-304, MUTAGENESIS OF SER-299; SER-302; SER-303 AND SER-304.
[14]	"Phosphorylation of chemoattractant receptors is not essential for chemotaxis or termination of G-protein-mediated responses." Kim J.-Y., Soede R.D.M., Schaap P., Valkema R., Borleis J.A., Van Haastert P.J.M., Devreotes P.N., Hereld D. J. Biol. Chem. 272:27313-27318(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF 288-LYS--ASN-392.
[15]	"Switching of chemoattractant receptors programs development and morphogenesis in Dictyostelium: receptor subtypes activate common responses at different agonist concentrations." Kim J.-Y., Borleis J.A., Devreotes P.N. Dev. Biol. 197:117-128(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASN-148; VAL-154; SER-155; PHE-156 AND THR-157.
[16]	"Desensitization of G-protein-coupled receptors. agonist-induced phosphorylation of the chemoattractant receptor cAR1 lowers its intrinsic affinity for cAMP." Xiao Z., Yao Y., Long Y., Devreotes P.N. J. Biol. Chem. 274:1440-1448(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[17]	"The phosphorylated C-terminus of cAR1 plays a role in cell-type-specific gene expression and STATa tyrosine phosphorylation." Briscoe C., Moniakis J., Kim J.-Y., Brown J.M., Hereld D., Devreotes P.N., Firtel R.A. Dev. Biol. 233:225-236(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[18]	"Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum development." Tsujioka M., Yokoyama M., Nishio K., Kuwayama H., Morio T., Katoh M., Urushihara H., Saito T., Ochiai H., Tanaka Y., Takeuchi I., Maeda M. Dev. Growth Differ. 43:275-283(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[19]	"cAMP receptor affinity controls wave dynamics, geometry and morphogenesis in Dictyostelium." Dormann D., Kim J.-Y., Devreotes P.N., Weijer C.J. J. Cell Sci. 114:2513-2523(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[20]	"A transcriptional profile of multicellular development in Dictyostelium discoideum." Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G. Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE [LARGE SCALE ANALYSIS].
[21]	"Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses." Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S., Morio T., Urushihara H., Tanaka Y., Loomis W.F. Eukaryot. Cell 2:627-637(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE [LARGE SCALE ANALYSIS].
[22]	"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum." Iranfar N., Fuller D., Loomis W.F. Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE [LARGE SCALE ANALYSIS].
[23]	"Control of cell type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization." Maruo T., Sakamoto H., Iranfar N., Fuller D., Morio T., Urushihara H., Tanaka Y., Maeda M., Loomis W.F. Eukaryot. Cell 3:1241-1248(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE [LARGE SCALE ANALYSIS].
[24]	"Constitutively active G protein-coupled receptor mutants block dictyostelium development." Zhang M., Goswami M., Hereld D. Mol. Biol. Cell 16:562-572(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF LEU-100; ILE-102; ILE-104; VAL-136 AND ASN-225.
[25]	"Regulation of G protein-coupled cAMP receptor activation by a hydrophobic residue in transmembrane helix 3." Zhang M., Goswami M., Sawai S., Cox E.C., Hereld D. Mol. Microbiol. 65:508-520(2007) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ILE-104.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21824 mRNA. Translation: AAA33177.1. AAFI02000010 Genomic DNA. Translation: EAL70653.1. AAFI02000010 Genomic DNA. Translation: EAL70721.1.
PIR	A41238.
RefSeq	XP_644603.1. XM_639511.1. XP_644648.1. XM_639556.1.
3D structure databases
ProteinModelPortal	P13773.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	P13773.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185024; DDB0185024; DDB_G0273397. DDB0238333; DDB0238333; DDB_G0273533.
GeneID	8618967. 8619010.
KEGG	ddi:DDB_G0273397. ddi:DDB_G0273533.
Organism-specific databases
dictyBase	DDB_G0273397. carA-1. DDB_G0273533. carA-2.
Phylogenomic databases
eggNOG	euNOG01404.
KO	K04629.
OMA	CLAISIY.
ProtClustDB	CLSZ2429917.
Family and domain databases
InterPro	IPR022343. GCR1-cAMP_receptor. IPR017981. GPCR_2-like. IPR000848. GPCR_cAMP. [Graphical view]
PRINTS	PR02001. GCR1CAMPR. PR00247. GPCRCAMP.
PROSITE	PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAR1_DICDI

Accession

Primary (citable) accession number: P13773
Secondary accession number(s): Q557I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents