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P13765

- DOB_HUMAN

UniProt

P13765 - DOB_HUMAN

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Protein

HLA class II histocompatibility antigen, DO beta chain

Gene

HLA-DOB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

GO - Molecular functioni

  1. MHC class II protein complex binding Source: UniProt
  2. MHC class II receptor activity Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  2. immune response Source: InterPro
  3. negative regulation of antigen processing and presentation of peptide antigen via MHC class II Source: UniProt
  4. signal transduction Source: GOC
Complete GO annotation...

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiREACT_121399. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
HLA class II histocompatibility antigen, DO beta chain
Alternative name(s):
MHC class II antigen DOB
Gene namesi
Name:HLA-DOB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:4937. HLA-DOB.

Subcellular locationi

Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein
Note: Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosomal membrane Source: Reactome
  4. lysosome Source: MGI
  5. MHC class II protein complex Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane, MHC II

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35061.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Add
BLAST
Chaini27 – 273247HLA class II histocompatibility antigen, DO beta chainPRO_0000018963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 105PROSITE-ProRule annotation
Glycosylationi45 – 451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi143 ↔ 199PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP13765.
PaxDbiP13765.
PRIDEiP13765.

PTM databases

PhosphoSiteiP13765.

Expressioni

Gene expression databases

BgeeiP13765.
CleanExiHS_HLA-DOB.
ExpressionAtlasiP13765. baseline.
GenevestigatoriP13765.

Organism-specific databases

HPAiHPA013846.

Interactioni

Subunit structurei

Heterodimer of an alpha chain (DOA) and a beta chain (DOB). Forms a heterotetrameric complex with an HLA-DM molecule during intracellular transport in endosomal/lysosomal compartments in B-cells.

Protein-protein interaction databases

BioGridi109357. 2 interactions.
DIPiDIP-60117N.
MINTiMINT-6630170.
STRINGi9606.ENSP00000395780.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 4411
Turni45 – 484
Beta strandi49 – 5810
Beta strandi61 – 677
Turni68 – 703
Beta strandi72 – 754
Helixi78 – 803
Helixi81 – 899
Helixi91 – 10313
Helixi105 – 1128
Helixi113 – 1153
Turni116 – 1183
Beta strandi124 – 1318
Beta strandi139 – 15113
Beta strandi154 – 1596
Beta strandi166 – 1694
Beta strandi177 – 1793
Beta strandi181 – 1899
Beta strandi197 – 2026
Beta strandi210 – 2145

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4I0PX-ray3.20D/H30-218[»]
ProteinModelPortaliP13765.
SMRiP13765. Positions 30-218.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 224198ExtracellularSequence AnalysisAdd
BLAST
Topological domaini246 – 27328CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei225 – 24521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini123 – 21391Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 12094Beta-1Add
BLAST
Regioni121 – 21494Beta-2Add
BLAST
Regioni215 – 22410Connecting peptide

Sequence similaritiesi

Belongs to the MHC class II family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG45668.
HOGENOMiHOG000100909.
HOVERGENiHBG012730.
InParanoidiP13765.
KOiK06752.
OMAiDAERWNN.
PhylomeDBiP13765.
TreeFamiTF336626.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view]
ProDomiPD000328. MHC_II_b_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13765-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSGWVPWVV ALLVNLTRLD SSMTQGTDSP EDFVIQAKAD CYFTNGTEKV
60 70 80 90 100
QFVVRFIFNL EEYVRFDSDV GMFVALTKLG QPDAEQWNSR LDLLERSRQA
110 120 130 140 150
VDGVCRHNYR LGAPFTVGRK VQPEVTVYPE RTPLLHQHNL LHCSVTGFYP
160 170 180 190 200
GDIKIKWFLN GQEERAGVMS TGPIRNGDWT FQTVVMLEMT PELGHVYTCL
210 220 230 240 250
VDHSSLLSPV SVEWRAQSEY SWRKMLSGIA AFLLGLIFLL VGIVIQLRAQ
260 270
KGYVRTQMSG NEVSRAVLLP QSC
Length:273
Mass (Da):30,822
Last modified:January 1, 1990 - v1
Checksum:iC06A1360DCC4AD26
GO

Polymorphismi

The following alleles of DOB are known: DOB*01:01, DOB*01:02, DOB*01:03 and DOB*01:04. The sequence shown is that of DOB*01:01.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti18 – 181R → Q in allele DOB*01:02.
Corresponds to variant rs2071554 [ dbSNP | Ensembl ].
VAR_016743
Natural varianti210 – 2101V → I.2 Publications
Corresponds to variant rs11575907 [ dbSNP | Ensembl ].
VAR_050363
Natural varianti234 – 2341L → F in allele DOB*01:04.
Corresponds to variant rs2070121 [ dbSNP | Ensembl ].
VAR_016745
Natural varianti244 – 2441V → I in allele DOB*01:03.
Corresponds to variant rs2621330 [ dbSNP | Ensembl ].
VAR_016744

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03066 mRNA. Translation: CAA26870.1.
M26040 mRNA. Translation: AAA59718.1.
X66401 Genomic DNA. Translation: CAA47028.1.
CR541832 mRNA. Translation: CAG46631.1.
CR788227 Genomic DNA. Translation: CAQ08588.1.
BC006097 mRNA. Translation: AAH06097.1.
L29472 Genomic DNA. Translation: AAA59717.1.
X87344 Genomic DNA. Translation: CAA60789.1.
AB035252 Genomic DNA. No translation available.
CCDSiCCDS4754.1.
PIRiS27335. A24669.
RefSeqiNP_002111.1. NM_002120.3.
UniGeneiHs.1802.

Genome annotation databases

EnsembliENST00000426644; ENSP00000395780; ENSG00000243612.
ENST00000438763; ENSP00000390020; ENSG00000241106.
ENST00000447178; ENSP00000405108; ENSG00000241386.
ENST00000454286; ENSP00000397268; ENSG00000243496.
ENST00000454969; ENSP00000410390; ENSG00000241910.
ENST00000456406; ENSP00000394783; ENSG00000239457.
GeneIDi3112.
KEGGihsa:3112.
UCSCiuc003oca.3. human.

Polymorphism databases

DMDMi122261.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03066 mRNA. Translation: CAA26870.1 .
M26040 mRNA. Translation: AAA59718.1 .
X66401 Genomic DNA. Translation: CAA47028.1 .
CR541832 mRNA. Translation: CAG46631.1 .
CR788227 Genomic DNA. Translation: CAQ08588.1 .
BC006097 mRNA. Translation: AAH06097.1 .
L29472 Genomic DNA. Translation: AAA59717.1 .
X87344 Genomic DNA. Translation: CAA60789.1 .
AB035252 Genomic DNA. No translation available.
CCDSi CCDS4754.1.
PIRi S27335. A24669.
RefSeqi NP_002111.1. NM_002120.3.
UniGenei Hs.1802.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4I0P X-ray 3.20 D/H 30-218 [» ]
ProteinModelPortali P13765.
SMRi P13765. Positions 30-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109357. 2 interactions.
DIPi DIP-60117N.
MINTi MINT-6630170.
STRINGi 9606.ENSP00000395780.

PTM databases

PhosphoSitei P13765.

Polymorphism databases

DMDMi 122261.

Proteomic databases

MaxQBi P13765.
PaxDbi P13765.
PRIDEi P13765.

Protocols and materials databases

DNASUi 3112.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000426644 ; ENSP00000395780 ; ENSG00000243612 .
ENST00000438763 ; ENSP00000390020 ; ENSG00000241106 .
ENST00000447178 ; ENSP00000405108 ; ENSG00000241386 .
ENST00000454286 ; ENSP00000397268 ; ENSG00000243496 .
ENST00000454969 ; ENSP00000410390 ; ENSG00000241910 .
ENST00000456406 ; ENSP00000394783 ; ENSG00000239457 .
GeneIDi 3112.
KEGGi hsa:3112.
UCSCi uc003oca.3. human.

Organism-specific databases

CTDi 3112.
GeneCardsi GC06M032780.
GC06Mi32764.
GC06Mj32702.
GC06Mk32758.
GC06Mm32814.
GC06Mo32870.
H-InvDB HIX0207728.
HIX0207758.
HGNCi HGNC:4937. HLA-DOB.
HPAi HPA013846.
MIMi 142920. gene.
600629. gene.
neXtProti NX_P13765.
PharmGKBi PA35061.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG45668.
HOGENOMi HOG000100909.
HOVERGENi HBG012730.
InParanoidi P13765.
KOi K06752.
OMAi DAERWNN.
PhylomeDBi P13765.
TreeFami TF336626.

Enzyme and pathway databases

Reactomei REACT_121399. MHC class II antigen presentation.

Miscellaneous databases

GeneWikii HLA-DOB.
GenomeRNAii 3112.
NextBioi 12349.
PROi P13765.
SOURCEi Search...

Gene expression databases

Bgeei P13765.
CleanExi HS_HLA-DOB.
ExpressionAtlasi P13765. baseline.
Genevestigatori P13765.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view ]
Pfami PF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view ]
ProDomi PD000328. MHC_II_b_N. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view ]
SUPFAMi SSF54452. SSF54452. 1 hit.
PROSITEi PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DO beta: a new beta chain gene in HLA-D with a distinct regulation of expression."
    Tonnelle C., Demars R., Long E.O.
    EMBO J. 4:2839-2847(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DOB*01:01).
  2. "Human class II DNA and DOB genes display low sequence variability."
    Jonsson A.-K., Rask L.
    Immunogenetics 29:411-413(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DOB*01:01).
  3. "DNA sequence analysis of 66 kb of the human MHC class II region encoding a cluster of genes for antigen processing."
    Beck S., Kelly A., Radley E., Khurshid F., Alderton R.P., Trowsdale J.
    J. Mol. Biol. 228:433-441(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE DOB*01:01).
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-210.
  5. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-210.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE DOB*01:01).
    Tissue: B-cell.
  7. "Class II genes of the human major histocompatibility complex. The DO beta gene is a divergent member of the class II beta gene family."
    Servenius B., Rask L., Peterson P.A.
    J. Biol. Chem. 262:8759-8766(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE DOB*01:02).
  8. "Evolutionary dynamics of non-coding sequences within the class II region of the human MHC."
    Beck S., Abdulla S., Alderton R.P., Glynne R.J., Gut I.G., Hosking L.K., Jackson A., Kelly A., Newell W.R., Sanseau P., Radley E., Thorpe K.L., Trowsdale J.
    J. Mol. Biol. 255:1-13(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE DOB*01:03).
  9. "The HLA-DOB gene displays limited polymorphism with only one amino acid substitution."
    Naruse T.K., Kawata H., Inoko H., Isshiki G., Yamano K., Hino M., Tatsumi N.
    Tissue Antigens 59:512-519(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-273 (ALLELE DOB*01:04).
  10. Cited for: REVIEW.

Entry informationi

Entry nameiDOB_HUMAN
AccessioniPrimary (citable) accession number: P13765
Secondary accession number(s): B0V0Y0
, Q29746, Q29825, Q6FHC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3