P13747 (HLAE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, alpha chain E

Alternative name(s):
MHC class I antigen E

Gene names

Name:	HLA-E
Synonyms:	HLA-6.2, HLAE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).
Subcellular location	Membrane; Single-pass type I membrane protein.
Polymorphism	The following alleles of E-1 are known: E*01:01, E*0102, E*01:03 and E*01:04. The sequence shown is that of E*01:01.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Caution	The existence of allele E*0102 (Ref.6) and allele E*0104 (Ref.10) is uncertain. The alleles could not be confirmed in further studies (Ref.14).

Ontologies

Keywords
Biological process	Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Traceable author statement. Source: Reactome integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Chain

22 – 358

337

HLA class I histocompatibility antigen, alpha chain E

PRO_0000018882

Regions

Topological domain

22 – 305

284

Extracellular Potential

Transmembrane

306 – 329

24

Helical; Potential

Topological domain

330 – 358

29

Cytoplasmic Potential

Domain

206 – 294

89

Ig-like C1-type

Region

22 – 111

90

Alpha-1

Region

112 – 203

92

Alpha-2

Region

204 – 295

92

Alpha-3

Region

296 – 305

10

Connecting peptide

Amino acid modifications

Glycosylation

107

1

N-linked (GlcNAc...) Ref.11

Disulfide bond

122 ↔ 185

Disulfide bond

224 ↔ 280

Natural variations

Natural variant

98

1

N → K.
Corresponds to variant rs1059510 [ dbSNP | Ensembl ].

VAR_059510

Natural variant

104

1

G → R in allele E*0102.

VAR_004395

Natural variant

128

1

R → G in allele E*01:03 and allele E*01:04.
Corresponds to variant rs1264457 [ dbSNP | Ensembl ].

VAR_016651

Natural variant

178

1

R → G in allele E*01:04.
Corresponds to variant rs41562314 [ dbSNP | Ensembl ].

VAR_016652

Experimental info

Sequence conflict

10

1

L → S in AAA52655. Ref.1

Secondary structure

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358

Helix Strand Turn

Details...

Beta strand

24 – 33

10

Beta strand

36 – 40

5

Beta strand

42 – 49

8

Beta strand

52 – 58

7

Beta strand

61 – 63

3

Beta strand

65 – 68

4

Helix

71 – 73

3

Helix

78 – 105

28

Beta strand

110 – 112

3

Beta strand

115 – 124

10

Beta strand

128 – 139

12

Beta strand

142 – 147

6

Beta strand

151 – 158

8

Helix

159 – 161

3

Helix

162 – 170

9

Turn

171 – 173

3

Helix

174 – 182

9

Helix

184 – 195

12

Helix

197 – 200

4

Beta strand

207 – 214

8

Beta strand

216 – 232

17

Beta strand

235 – 239

5

Turn

241 – 243

3

Beta strand

249 – 251

3

Beta strand

258 – 260

3

Beta strand

262 – 271

10

Helix

275 – 277

3

Beta strand

279 – 283

5

Beta strand

287 – 289

3

Beta strand

291 – 293

3

Sequences

	Sequence		Length	Mass (Da)
	P13747 [UniParc]. Last modified August 29, 2003. Version 3. Checksum: 2A211B603591D248	FASTA	358	40,157
	10 20 30 40 50 60 MVDGTLLLLL SEALALTQTW AGSHSLKYFH TSVSRPGRGE PRFISVGYVD DTQFVRFDND 70 80 90 100 110 120 AASPRMVPRA PWMEQEGSEY WDRETRSARD TAQIFRVNLR TLRGYYNQSE AGSHTLQWMH 130 140 150 160 170 180 GCELGPDRRF LRGYEQFAYD GKDYLTLNED LRSWTAVDTA AQISEQKSND ASEAEHQRAY 190 200 210 220 230 240 LEDTCVEWLH KYLEKGKETL LHLEPPKTHV THHPISDHEA TLRCWALGFY PAEITLTWQQ 250 260 270 280 290 300 DGEGHTQDTE LVETRPAGDG TFQKWAAVVV PSGEEQRYTC HVQHEGLPEP VTLRWKPASQ 310 320 330 340 350 PTIPIVGIIA GLVLLGSVVS GAVVAAVIWR KKSSGGKGGS YSKAEWSDSA QGSESHSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and nucleotide sequence of a cDNA clone encoding a novel HLA class I gene." Mizuno S., Trapani J.A., Koller B.H., Dupont B., Yang S.Y. J. Immunol. 140:4024-4030(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE E*01:01).
[2]	"Cell surface expression of HLA-E: interaction with human beta-2 microglobulin and allelic differences." Ulbrecht M., Courturier A., Martinozzi S., Pla M., Srivastava R., Peterson P.A., Weiss E.H. Eur. J. Immunol. 29:537-547(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELES E*01:01 AND E*01:03).
[3]	"HLA-E, F, and G polymorphism: genomic sequence defines new variation spanning the nonclassical class I genes." Ishitani A., Miki A., Williams L.M., Moore Y., Geraghty D.E. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELES E*01:01 AND E*01:03).
[4]	"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region." Shiina S., Tamiya G., Oka A., Inoko H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE E*01:01).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE E*01:01). Tissue: Ovarian adenocarcinoma and Pancreas.
[6]	"HLA-E. A novel HLA class I gene expressed in resting T lymphocytes." Koller B.H., Geraghty D.E., Shimizu Y., Demars R., Orr H.T. J. Immunol. 141:897-904(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE E*0102).
[7]	Ulbrecht M. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELE E*01:03).
[8]	"A new variant of HLA-E*010303 with three synonymous mutations." He X., Xu L., Liu Y., Zeng Y. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE E*01:03).
[9]	"Cloning of HLA-E cDNA from activated peripheral leukocytes." He X., Xu L., Liu Y., Zeng Y. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE E*01:03).
[10]	"Polymorphism in the human class I MHC locus HLA-E in Japanese." Ohya K., Kondo K., Mizuno S. Immunogenetics 32:205-209(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-203 (ALLELE E*01:04). Tissue: Peripheral blood.
[11]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-107, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Structural features impose tight peptide binding specificity in the nonclassical MHC molecule HLA-E." O'Callaghan C.A., Tormo J., Willcox B.E., Braud V.M., Jakobsen B.K., Stuart D.I., McMichael A.J., Bell J.I., Jones E.Y. Mol. Cell 1:531-541(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 22-295.
[14]	"Definitive high resolution typing of HLA-E allelic polymorphisms: identifying potential errors in existing allele data." Grimsley C., Kawasaki A., Gassner C., Sageshima N., Nose Y., Hatake K., Geraghty D.E., Ishitani A. Tissue Antigens 60:206-212(2002) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM, QUESTIONING ON ALLELES E*0102 AND E*01:04.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M20022 mRNA. Translation: AAA52655.1.
AJ293263 mRNA. Translation: CAC07212.1.
AJ293264 mRNA. Translation: CAC07213.1.
AF523274 Genomic DNA. Translation: AAM74969.1.
AF523275 Genomic DNA. Translation: AAM74970.1.
AF523276 Genomic DNA. Translation: AAM74971.1.
AF523277 Genomic DNA. Translation: AAM74972.1.
AF523278 Genomic DNA. Translation: AAM74973.1.
AF523279 Genomic DNA. Translation: AAM74974.1.
AF523280 Genomic DNA. Translation: AAM74975.1.
AF523281 Genomic DNA. Translation: AAM74976.1.
AF523282 Genomic DNA. Translation: AAM74977.1.
AF523283 Genomic DNA. Translation: AAM74978.1.
BA000025 Genomic DNA. Translation: BAB63328.1.
BC002578 mRNA. Translation: AAH02578.1.
BC004297 mRNA. Translation: AAH04297.1.
M21533 Genomic DNA. Translation: AAA59835.1.
AY221103 mRNA. Translation: AAO34408.1.
AY216681 mRNA. Translation: AAO37688.1.
M32508 Genomic DNA. Translation: AAA63225.1.

IPI

IPI00718879.

PIR

A28834.

RefSeq

NP_005507.3. NM_005516.5.

UniGene

Hs.650174.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KPR	X-ray	2.80	A/C	22-295	[»]
1KTL	X-ray	3.10	A/C	22-295	[»]
1MHE	X-ray	2.85	A/C	22-295	[»]
2ESV	X-ray	2.60	A	23-297	[»]
3AM8	X-ray	2.80	A/B	22-297	[»]
3BZE	X-ray	2.50	A/C/E/G	23-295	[»]
3BZF	X-ray	2.50	A/C	22-297	[»]
3CDG	X-ray	3.40	A/C	23-295	[»]
3CII	X-ray	4.41	A/D	23-295	[»]

ProteinModelPortal

P13747.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-32N.

IntAct

P13747. 18 interactions.

STRING

9606.ENSP00000373091.

PTM databases

PhosphoSite

P13747.

Polymorphism databases

DMDM

34395942.

Proteomic databases

PaxDb

P13747.

PRIDE

P13747.

Protocols and materials databases

DNASU

3133.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000383597; ENSP00000373091; ENSG00000206493.
ENST00000415289; ENSP00000409910; ENSG00000229252.
ENST00000415649; ENSP00000390707; ENSG00000233904.
ENST00000427936; ENSP00000397420; ENSG00000230254.

GeneID

3133.

KEGG

hsa:3133.

Organism-specific databases

CTD

3133.

GeneCards

GC06P030457.

HGNC

HGNC:4962. HLA-E.

HPA

CAB024589.
HPA031454.

MIM

143010. gene.

neXtProt

NX_P13747.

PharmGKB

PA35081.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG329718.

HOVERGEN

HBG016709.

InParanoid

P13747.

KO

K06751.

OrthoDB

EOG4F4SBG.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P13747.

CleanEx

HS_HLA-E.

Genevestigator

P13747.

GermOnline

ENSG00000204592. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.30.500.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]

PRINTS

PR01638. MHCCLASSI.

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

HLA-E. human.

EvolutionaryTrace

P13747.

GenomeRNAi

3133.

NextBio

12427.

SOURCE

Search...

Entry information

Entry name

HLAE_HUMAN

Accession

Primary (citable) accession number: P13747
Secondary accession number(s): Q30169 , Q9BT83, Q9GIY7, Q9GIY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	August 29, 2003
Last modified:	May 1, 2013
This is version 141 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.