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Protein

Glutathione S-transferase A1

Gene

Gsta1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9Glutathione1 Publication1
Binding sitei45Glutathione1 Publication1

GO - Molecular functioni

GO - Biological processi

  • glutathione metabolic process Source: UniProtKB
  • response to stilbenoid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A1 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 1
Glutathione S-transferase Ya
Glutathione S-transferase Ya1
Cleaved into the following chain:
Gene namesi
Name:Gsta1
Synonyms:Gsta, Gstya
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1095417. Gsta1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857881 – 223Glutathione S-transferase A1Add BLAST223
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004232042 – 223Glutathione S-transferase A1, N-terminally processedAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylalanine; in Glutathione S-transferase A1, N-terminally processedBy similarity1
Modified residuei4N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP13745.
PaxDbiP13745.
PeptideAtlasiP13745.
PRIDEiP13745.

PTM databases

iPTMnetiP13745.
PhosphoSitePlusiP13745.

Expressioni

Inductioni

Induced in the liver by beta-naphthoflavone (BNF) and 23-t-butyl-4-hydroxyanisole (BHA).1 Publication

Gene expression databases

BgeeiENSMUSG00000074183.
CleanExiMM_GSTA1.
GenevisibleiP13745. MM.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi200090. 1 interactor.
IntActiP13745. 3 interactors.
MINTiMINT-4096961.
STRINGi10090.ENSMUSP00000096139.

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Turni14 – 16Combined sources3
Helixi17 – 25Combined sources9
Beta strandi31 – 35Combined sources5
Helixi38 – 46Combined sources9
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Helixi86 – 108Combined sources23
Helixi109 – 111Combined sources3
Turni114 – 116Combined sources3
Helixi117 – 130Combined sources14
Helixi132 – 143Combined sources12
Beta strandi146 – 149Combined sources4
Helixi155 – 171Combined sources17
Helixi172 – 175Combined sources4
Helixi179 – 189Combined sources11
Helixi192 – 198Combined sources7
Helixi210 – 217Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F3AX-ray1.90A/B2-223[»]
1F3BX-ray2.00A/B2-223[»]
ProteinModelPortaliP13745.
SMRiP13745.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13745.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 208GST C-terminalAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione binding1 Publication2
Regioni67 – 68Glutathione binding1 Publication2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP13745.
KOiK00799.
OMAiAICKYIA.
OrthoDBiEOG091G0O3D.
PhylomeDBiP13745.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPVLHYF NARGRMECIR WLLAAAGVEF EEKFIQSPED LEKLKKDGNL
60 70 80 90 100
MFDQVPMVEI DGMKLAQTRA ILNYIATKYD LYGKDMKERA LIDMYSEGIL
110 120 130 140 150
DLTEMIGQLV LCPPDQREAK TALAKDRTKN RYLPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLTRVDIHL LEVLLYVEEF DASLLTPFPL LKAFKSRISS LPNVKKFLQP
210 220
GSQRKPPMDA KQIQEARKAF KIQ
Length:223
Mass (Da):25,608
Last modified:January 23, 2007 - v2
Checksum:iA348A07133FBEC1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19256
, M19251, M19252, M19253, M19254, M19255 Genomic DNA. Translation: AAA37750.1. Sequence problems.
BC132572 mRNA. Translation: AAI32573.1.
BC132576 mRNA. Translation: AAI32577.1.
CCDSiCCDS23358.1.
PIRiA27848.
RefSeqiNP_032207.3. NM_008181.3.
UniGeneiMm.467426.

Genome annotation databases

EnsembliENSMUST00000098537; ENSMUSP00000096139; ENSMUSG00000074183.
GeneIDi14857.
KEGGimmu:14857.
UCSCiuc009qtz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19256
, M19251, M19252, M19253, M19254, M19255 Genomic DNA. Translation: AAA37750.1. Sequence problems.
BC132572 mRNA. Translation: AAI32573.1.
BC132576 mRNA. Translation: AAI32577.1.
CCDSiCCDS23358.1.
PIRiA27848.
RefSeqiNP_032207.3. NM_008181.3.
UniGeneiMm.467426.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F3AX-ray1.90A/B2-223[»]
1F3BX-ray2.00A/B2-223[»]
ProteinModelPortaliP13745.
SMRiP13745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200090. 1 interactor.
IntActiP13745. 3 interactors.
MINTiMINT-4096961.
STRINGi10090.ENSMUSP00000096139.

PTM databases

iPTMnetiP13745.
PhosphoSitePlusiP13745.

Proteomic databases

MaxQBiP13745.
PaxDbiP13745.
PeptideAtlasiP13745.
PRIDEiP13745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098537; ENSMUSP00000096139; ENSMUSG00000074183.
GeneIDi14857.
KEGGimmu:14857.
UCSCiuc009qtz.1. mouse.

Organism-specific databases

CTDi2938.
MGIiMGI:1095417. Gsta1.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP13745.
KOiK00799.
OMAiAICKYIA.
OrthoDBiEOG091G0O3D.
PhylomeDBiP13745.
TreeFamiTF105321.

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.

Miscellaneous databases

EvolutionaryTraceiP13745.
PROiP13745.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074183.
CleanExiMM_GSTA1.
GenevisibleiP13745. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA1_MOUSE
AccessioniPrimary (citable) accession number: P13745
Secondary accession number(s): A2RTN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.