Reviewed, UniProtKB/Swiss-Prot P13745 (GSTA1_MOUSE)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase A1
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Ya
    Ya1
    GST class-alpha member 1

Gene names

Name:	Gsta1
Synonyms:	Gsta, Gstya

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Induction	Induced in the liver by beta-naphthoflavone (BNF) and 23-t-butyl-4-hydroxyanisole (BHA). Ref.3
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 223

222

Glutathione S-transferase A1

PRO_0000185788

Regions

Domain

3 – 83

81

GST N-terminal

Domain

85 – 208

124

GST C-terminal

Secondary structure

1

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223

Helix Strand Turn

Details...

Beta strand

6 – 12

7

Turn

14 – 16

3

Helix

17 – 25

9

Beta strand

31 – 35

5

Helix

38 – 46

9

Beta strand

57 – 60

4

Beta strand

63 – 67

5

Helix

68 – 78

11

Helix

86 – 108

23

Helix

109 – 111

3

Turn

114 – 116

3

Helix

117 – 130

14

Helix

132 – 143

12

Beta strand

146 – 149

4

Helix

155 – 171

17

Helix

172 – 175

4

Helix

179 – 189

11

Helix

192 – 198

7

Helix

210 – 217

8

Sequences

	Sequence		Length	Mass (Da)
	P13745-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A348A07133FBEC1D	FASTA	223	25,608
	10 20 30 40 50 60 MAGKPVLHYF NARGRMECIR WLLAAAGVEF EEKFIQSPED LEKLKKDGNL MFDQVPMVEI 70 80 90 100 110 120 DGMKLAQTRA ILNYIATKYD LYGKDMKERA LIDMYSEGIL DLTEMIGQLV LCPPDQREAK 130 140 150 160 170 180 TALAKDRTKN RYLPAFEKVL KSHGQDYLVG NRLTRVDIHL LEVLLYVEEF DASLLTPFPL 190 200 210 220 LKAFKSRISS LPNVKKFLQP GSQRKPPMDA KQIQEARKAF KIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse glutathione S-transferase Ya subunit: gene structure and sequence." Daniel V., Sharon R., Tichauer Y., Sarid S. DNA 6:317-324(1987) [PubMed: 3652905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Regulation of mouse glutathione S-transferases by chemoprotectors. Molecular evidence for the existence of three distinct alpha-class glutathione S-transferase subunits, Ya1, Ya2, and Ya3, in mouse liver." McLellan L.I., Kerr L.A., Cronshaw A.D., Hayes J.D. Biochem. J. 276:461-469(1991) [PubMed: 2049074] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-36; 51-59; 63-165 AND 208-223, INDUCTION. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M19256 , M19251, M19252, M19253, M19254, M19255 Genomic DNA. Translation: AAA37750.1. Sequence problems.
BC132572 mRNA. Translation: AAI32573.1.
BC132576 mRNA. Translation: AAI32577.1.

IPI

IPI00554953.

PIR

A27848.

RefSeq

NP_032207.3.

UniGene

Mm.467426

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F3A	X-ray	1.90	A/B	2-222	[»]
1F3B	X-ray	2.00	A/B	2-222	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P13745.

Proteomic databases

PRIDE

P13745.

Genome annotation databases

Ensembl

ENSMUST00000098537; ENSMUSP00000096139; ENSMUSG00000074183; Mus musculus. [Genome view]

GeneID

14857.

KEGG

mmu:14857.

UCSC

uc009qtz.1. mouse.

Organism-specific databases

CTD

14857.

MGI

MGI:1095417. Gsta1.

Phylogenomic databases

HOGENOM

P13745.

HOVERGEN

P13745.

OMA

QSHGQDY.

Enzyme and pathway databases

BRENDA

2.5.1.18. 244.

Gene expression databases

Bgee

P13745.

CleanEx

MM_GSTA1.

Genevestigator

P13745.

GermOnline

ENSMUSG00000074183. Mus musculus.

Family and domain databases

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

PANTHER

PTHR11571:SF4. GST_alpha. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

PRINTS

PR01266. GSTRNSFRASEA.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00143. Glutathione.

NextBio

287087.

SOURCE

Search...

Entry information

Entry name

GSTA1_MOUSE

Accession

Primary (citable) accession number: P13745
Secondary accession number(s): A2RTN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)